Rh3AG213600

Pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0009701
Physical Location & Seq
Reverse (-)
21542205 .. 21548165
5961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG213600.1

Sequence Viewer

Length: 672 bp
ATGGGTAAACCAAACCAAAGAGTTTGGGAACATGAATGGAGAAAACACGGTTCATGTACCACAGATTTATTACCAACTTCTAATGACTACCTACTGAAAGGAGTTTACCTGTGGGAGCAAATCAAGTTTCATGATTTGATGGGTGTGGGTCCGGGTGCCCAATTTAAGCCAAATACTCAATATGATGCGAACACGGTGCTGGCGGCCATCACAAGCAAATACAAGGTCAAAACAATGCTGACCTGTCAGGCTGGGGAAATATTGGATGTTAGGTTTTGTTATTTAGGACCTTGGGTATTGGTAGATTGCCTGGACACCCAACCACCTCCCCATACAGTTTGTACAGGTCTTCTTGACAAGGCCGAAGCTATTATTGAAAGGGTTACACTGAAGGGTGCGAAACCTGATGCAAAGTCATGGTATTCTTTGGCAAAAGTGCATCCTGATCATGATCAAACTGAGAAGGCAATTGAGTTGACGAGAAAAGCACTTTTGGCTGTGCAGCCTGGTTGGAAGACCAACACGGATGTTTGGGCTGCCTGTCTCAACTACTTGATAATGAAACCTGATCCAGAGGGTGCCAAGGAATATATAAAGCTCCTCACCGATGAGAATGTTATCACCGTGATTACACAAGAGAGACTGTTGAATAAGATGAATAAGACAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

223

Amino Acids

25.24

Weight (kDa)

6.99

Isoelectric Point (pI)

18.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 5 - 94 1.9e-11 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 155, 578
AciI CCGC 1 cut(s) 203
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 204
AcuI CTGAAG 1 cut(s) 410
AfaI GTAC 2 cut(s) 58, 343
AgsI TTSAA 2 cut(s) 377, 649
AjnI CCWGG 2 cut(s) 309, 505
AluBI AGCT 2 cut(s) 368, 598
AluI AGCT 2 cut(s) 368, 598
Alw26I GTCTC 2 cut(s) 548, 634
AlwI GGATC 1 cut(s) 563
AoxI GGCC 2 cut(s) 204, 360
ApeKI GCWGC 2 cut(s) 502, 536
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
AspS9I GGNCC 2 cut(s) 149, 287
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 2 cut(s) 595, 613
AvaII GGWCC 2 cut(s) 149, 287
BaeGI GKGCMC 1 cut(s) 160
BanI GGYRCC 2 cut(s) 155, 578
BbsI GAAGAC 2 cut(s) 341, 521
BbvI GCAGC 2 cut(s) 514, 523
BccI CCATC 2 cut(s) 133, 215
BcgI CGANNNNNNTGC 2 cut(s) 178, 212
BciT130I CCWGG 2 cut(s) 311, 507
BclI TGATCA 2 cut(s) 445, 451
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 2 cut(s) 548, 634
BisI GCNGC 3 cut(s) 204, 503, 537
BlsI GCNGC 3 cut(s) 205, 504, 538
Bme1390I CCNGG 3 cut(s) 153, 311, 507
Bme18I GGWCC 2 cut(s) 149, 287
BmgT120I GGNCC 2 cut(s) 149, 287
BmiI GGNNCC 3 cut(s) 150, 157, 580
BmrFI CCNGG 3 cut(s) 153, 311, 507
BmsI GCATC 3 cut(s) 175, 397, 448
BpiI GAAGAC 2 cut(s) 341, 521
BpuMI CCSGG 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 290, 582
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 2 cut(s) 311, 507
BseDI CCNNGG 2 cut(s) 290, 582
BseGI GGATG 3 cut(s) 271, 439, 532
BseJI GATNNNNATC 1 cut(s) 450
BseMII CTCAG 1 cut(s) 450
BseRI GAGGAG 1 cut(s) 590
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 2 cut(s) 514, 523
BseYI CCCAGC 1 cut(s) 251
BsgI GTGCAG 1 cut(s) 521
BshFI GGCC 2 cut(s) 206, 362
BshNI GGYRCC 2 cut(s) 155, 578
BsiSI CCGG 1 cut(s) 152
BsmAI GTCTC 2 cut(s) 548, 634
BsnI GGCC 2 cut(s) 206, 362
Bsp1286I GDGCHC 1 cut(s) 160
Bsp1407I TGTACA 1 cut(s) 341
Bsp143I GATC 3 cut(s) 445, 451, 568
BspACI CCGC 1 cut(s) 203
BspANI GGCC 2 cut(s) 206, 362
BspCNI CTCAG 1 cut(s) 451
BspHI TCATGA 2 cut(s) 130, 448
BspLI GGNNCC 3 cut(s) 150, 157, 580
BspPI GGATC 1 cut(s) 563
BspT107I GGYRCC 2 cut(s) 155, 578
BsrGI TGTACA 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 290, 582
BssMI GATC 3 cut(s) 445, 451, 568
BssT1I CCWWGG 2 cut(s) 290, 582
Bst2UI CCWGG 2 cut(s) 311, 507
Bst4CI ACNGT 5 cut(s) 50, 196, 337, 625, 645
BstAUI TGTACA 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 459
BstF5I GGATG 3 cut(s) 271, 439, 532
BstKTI GATC 3 cut(s) 448, 454, 571
BstMAI GTCTC 2 cut(s) 548, 634
BstMBI GATC 3 cut(s) 445, 451, 568
BstMWI GCNNNNNNNGC 1 cut(s) 494
BstNI CCWGG 2 cut(s) 311, 507
BstSCI CCNGG 3 cut(s) 151, 309, 505
BstSLI GKGCMC 1 cut(s) 160
BstV1I GCAGC 2 cut(s) 514, 523
BstV2I GAAGAC 2 cut(s) 341, 521
BsuRI GGCC 2 cut(s) 206, 362
BtsCI GGATG 3 cut(s) 271, 439, 532
BtsIMutI CAGTG 1 cut(s) 386
Cac8I GCNNGC 1 cut(s) 201
CciI TCATGA 2 cut(s) 130, 448
Cfr13I GGNCC 2 cut(s) 149, 287
Csp6I GTAC 2 cut(s) 57, 342
CviAII CATG 5 cut(s) 32, 54, 131, 417, 449
CviJI RGCY 9 cut(s) 169, 206, 251, 362, 368, 497, 505, 536, 598
CviKI_1 RGCY 9 cut(s) 169, 206, 251, 362, 368, 497, 505, 536, 598
CviQI GTAC 2 cut(s) 57, 342
DdeI CTNAG 1 cut(s) 459
DpnI GATC 3 cut(s) 447, 453, 570
DpnII GATC 3 cut(s) 445, 451, 568
EaeI YGGCCR 1 cut(s) 204
Eco130I CCWWGG 2 cut(s) 290, 582
Eco47I GGWCC 2 cut(s) 149, 287
Eco57I CTGAAG 1 cut(s) 410
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 309, 505
EcoT14I CCWWGG 2 cut(s) 290, 582
ErhI CCWWGG 2 cut(s) 290, 582
FaeI CATG 5 cut(s) 35, 57, 134, 420, 452
FaiI YATR 9 cut(s) 33, 55, 132, 183, 333, 418, 450, 591, 593
FatI CATG 5 cut(s) 31, 53, 130, 416, 448
FbaI TGATCA 2 cut(s) 445, 451
Fnu4HI GCNGC 3 cut(s) 204, 503, 537
FokI GGATG 3 cut(s) 278, 426, 539
Fsp4HI GCNGC 3 cut(s) 204, 503, 537
GluI GCNGC 3 cut(s) 204, 503, 537
GsaI CCCAGC 1 cut(s) 255
HaeIII GGCC 2 cut(s) 206, 362
HapII CCGG 1 cut(s) 152
Hin1II CATG 5 cut(s) 35, 57, 134, 420, 452
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HpaII CCGG 1 cut(s) 152
HphI GGTGA 2 cut(s) 595, 613
Hpy166II GTNNAC 3 cut(s) 8, 106, 477
Hpy188III TCNNGA 5 cut(s) 131, 353, 443, 449, 572
Hpy8I GTNNAC 3 cut(s) 8, 106, 477
HpyAV CCTTC 2 cut(s) 385, 457
HpyCH4III ACNGT 5 cut(s) 50, 196, 337, 625, 645
HpyCH4V TGCA 3 cut(s) 410, 439, 502
HpyF10VI GCNNNNNNNGC 1 cut(s) 494
HpyF3I CTNAG 1 cut(s) 459
Hsp92II CATG 5 cut(s) 35, 57, 134, 420, 452
Ksp22I TGATCA 2 cut(s) 445, 451
Kzo9I GATC 3 cut(s) 445, 451, 568
LmnI GCTCC 2 cut(s) 115, 603
Lsp1109I GCAGC 2 cut(s) 514, 523
LweI GCATC 3 cut(s) 175, 397, 448
MaeIII GTNAC 1 cut(s) 382
MalI GATC 3 cut(s) 447, 453, 570
MboI GATC 3 cut(s) 445, 451, 568
MboII GAAGA 2 cut(s) 341, 526
MfeI CAATTG 1 cut(s) 468
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 2 cut(s) 161, 468
MmeI TCCRAC 1 cut(s) 491
MnlI CCTC 3 cut(s) 336, 568, 611
MseI TTAA 1 cut(s) 165
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 3 cut(s) 153, 311, 507
MunI CAATTG 1 cut(s) 468
MvaI CCWGG 2 cut(s) 311, 507
MwoI GCNNNNNNNGC 1 cut(s) 494
NciI CCSGG 1 cut(s) 153
NdeII GATC 3 cut(s) 445, 451, 568
NlaIII CATG 5 cut(s) 35, 57, 134, 420, 452
NlaIV GGNNCC 3 cut(s) 150, 157, 580
PagI TCATGA 2 cut(s) 130, 448
PkrI GCNGC 3 cut(s) 205, 504, 538
PpuMI RGGWCCY 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 309, 505
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 2 cut(s) 309, 505
PspN4I GGNNCC 3 cut(s) 150, 157, 580
PspPI GGNCC 2 cut(s) 149, 287
PspPPI RGGWCCY 1 cut(s) 287
RsaI GTAC 2 cut(s) 58, 343
RsaNI GTAC 2 cut(s) 57, 342
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 3 cut(s) 204, 503, 537
Sau3AI GATC 3 cut(s) 445, 451, 568
Sau96I GGNCC 2 cut(s) 149, 287
ScrFI CCNGG 3 cut(s) 153, 311, 507
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 3 cut(s) 175, 397, 448
SinI GGWCC 2 cut(s) 149, 287
Sse9I AATT 2 cut(s) 161, 468
SsiI CCGC 1 cut(s) 203
SspI AATATT 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 151, 309, 505
StyI CCWWGG 2 cut(s) 290, 582
TaaI ACNGT 5 cut(s) 50, 196, 337, 625, 645
TasI AATT 2 cut(s) 161, 468
TatI WGTACW 1 cut(s) 341
TauI GCSGC 1 cut(s) 206
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 1 cut(s) 393
TseI GCWGC 2 cut(s) 502, 536
TspDTI ATGAA 5 cut(s) 42, 48, 119, 575, 671
TspGWI ACGGA 1 cut(s) 539
TspRI CASTG 1 cut(s) 393
VpaK11BI GGWCC 2 cut(s) 149, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.