RLG00000023532

Ribonuclease T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009701
Physical Location & Seq
Reverse (-)
24980252 .. 24984136
3885 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023532

Sequence Viewer

Length: 828 bp
ATGCTGCTGCTGAAACAATCCAAGCCTGAGGGAAAAGAGGAGAGTGAAGAGCTGAAAAGCCTGGGCCTATTATATCTAAGTATGTATCATATTTGGATATCTTGTATCACGTCTTCTTCCTTTCCGAGCTTTGTTGCCAAGGCCTGGGAAGTAGGTTTTCTCTGGGACTCTGGAAGTGGTGGATCTGAGGCTGATCTGGGCAAAGGTATCCAACATGGATATATTGCAGGAGCTGTTGAGATTGTTAATGATGGGTCTGAGCCTCTGAGTCGAACTAGTGACCAGATGACTTTGAGAGCCACCAAGGCACATGGGGACCGGCACAGTGAACCTATGCTAAATCCTTCGACCTTTCGTGGCGACGCTCCTTGGGGCATACATGGTATCTGGGCAGAAAAACTTGAACCACTTCCAGGCCCTCTACGAATGTATACGTATGTGCCGGTTGCATGGTGTCAATCATTTCCTCCATTTGACCCGAAGCTTAGTCAAAACCTTATAAGTGTACTCGATACAGCTTGGCCGAGTATTTCAACGCAGTGGACAGATCTTCAAATTTGGGAATATGAATATAATGAACACGGTTCATGTACCGTAGATTTATTACCAACTTCTACGGATTACATAATGAAAGCACTTTACCTGTGGGAGGAACTCAAGTTTGACAAGGTGATGGGTGAGGGTAACCAATTTGAGCCAAATACCCTATACGAAACCGAGGAGATTCTTAAGGGCATCAAAGGTTCATATAAAGATTCGAGCCCCCACAATCGTCGGATTAGAAGTAAGACGCCTTATCCACTAGGCCACGAGCTTCCTTGCTTGTAG

Protein Analysis

276

Amino Acids

31.02

Weight (kDa)

5.13

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 117 - 251 3.5e-15 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AccB7I CCANNNNNTGG 1 cut(s) 144
AccI GTMKAC 1 cut(s) 431
AclWI GGATC 1 cut(s) 190
AcoI YGGCCR 1 cut(s) 521
AcsI RAATTY 1 cut(s) 555
AcyI GRCGYC 1 cut(s) 791
AfaI GTAC 2 cut(s) 507, 592
AfiI CCNNNNNNNGG 3 cut(s) 144, 413, 649
AflII CTTAAG 1 cut(s) 728
AgsI TTSAA 3 cut(s) 404, 534, 554
AhlI ACTAGT 1 cut(s) 275
AjiI CACGTC 1 cut(s) 111
AjnI CCWGG 3 cut(s) 60, 143, 412
AluBI AGCT 6 cut(s) 52, 129, 233, 484, 518, 814
AluI AGCT 6 cut(s) 52, 129, 233, 484, 518, 814
AlwI GGATC 1 cut(s) 190
AlwNI CAGNNNCTG 1 cut(s) 233
AoxI GGCC 5 cut(s) 64, 141, 415, 521, 805
ApeKI GCWGC 2 cut(s) 4, 7
ApoI RAATTY 1 cut(s) 555
Asp700I GAANNNNTTC 1 cut(s) 408
AspS9I GGNCC 3 cut(s) 64, 316, 416
AsuHPI GGTGA 2 cut(s) 682, 689
AvaII GGWCC 1 cut(s) 316
AxyI CCTNAGG 1 cut(s) 27
BanII GRGCYC 1 cut(s) 764
BarI GAAGNNNNNNTAC 2 cut(s) 97, 129
BauI CACGAG 1 cut(s) 809
BbsI GAAGAC 1 cut(s) 105
BccI CCATC 2 cut(s) 245, 667
BciT130I CCWGG 3 cut(s) 62, 145, 414
BciVI GTATCC 1 cut(s) 218
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 2 cut(s) 276, 803
BfrI CTTAAG 1 cut(s) 728
BfuI GTATCC 1 cut(s) 218
BglI GCCNNNNNGGC 1 cut(s) 305
BglII AGATCT 1 cut(s) 547
BisI GCNGC 2 cut(s) 5, 8
BlsI GCNGC 2 cut(s) 6, 9
Bme1390I CCNGG 3 cut(s) 62, 145, 414
Bme18I GGWCC 1 cut(s) 316
BmgBI CACGTC 1 cut(s) 111
BmgT120I GGNCC 3 cut(s) 64, 316, 416
BmiI GGNNCC 1 cut(s) 317
BmrFI CCNGG 3 cut(s) 62, 145, 414
BmsI GCATC 1 cut(s) 744
BpiI GAAGAC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 641
BsaAI YACGTR 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 791
BsaJI CCNNGG 6 cut(s) 61, 138, 144, 303, 368, 717
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 413, 649
Bse118I RCCGGY 2 cut(s) 318, 442
Bse21I CCTNAGG 1 cut(s) 27
BseBI CCWGG 3 cut(s) 62, 145, 414
BseDI CCNNGG 6 cut(s) 61, 138, 144, 303, 368, 717
BseLI CCNNNNNNNGG 3 cut(s) 144, 413, 649
BseMII CTCAG 4 cut(s) 18, 177, 249, 257
BseRI GAGGAG 2 cut(s) 53, 734
BshFI GGCC 5 cut(s) 66, 143, 417, 523, 807
BsiSI CCGG 2 cut(s) 319, 443
BslFI GGGAC 2 cut(s) 179, 329
BslI CCNNNNNNNGG 3 cut(s) 144, 413, 649
BsmFI GGGAC 2 cut(s) 179, 329
BsnI GGCC 5 cut(s) 66, 143, 417, 523, 807
Bsp1286I GDGCHC 1 cut(s) 764
Bsp143I GATC 3 cut(s) 182, 193, 547
BspANI GGCC 5 cut(s) 66, 143, 417, 523, 807
BspCNI CTCAG 4 cut(s) 19, 178, 250, 258
BspLI GGNNCC 1 cut(s) 317
BspPI GGATC 1 cut(s) 190
BspQI GCTCTTC 1 cut(s) 42
BspTI CTTAAG 1 cut(s) 728
BsrFI RCCGGY 2 cut(s) 318, 442
BssAI RCCGGY 2 cut(s) 318, 442
BssECI CCNNGG 6 cut(s) 61, 138, 144, 303, 368, 717
BssMI GATC 3 cut(s) 182, 193, 547
BssNAI GTATAC 1 cut(s) 432
BssNI GRCGYC 1 cut(s) 791
BssSI CACGAG 1 cut(s) 809
BssT1I CCWWGG 3 cut(s) 138, 303, 368
Bst1107I GTATAC 1 cut(s) 432
Bst2BI CACGAG 1 cut(s) 809
Bst2UI CCWGG 3 cut(s) 62, 145, 414
Bst4CI ACNGT 3 cut(s) 326, 584, 595
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 791
BstAFI CTTAAG 1 cut(s) 728
BstBAI YACGTR 1 cut(s) 435
BstDEI CTNAG 6 cut(s) 27, 77, 186, 258, 266, 485
BstEII GGTNACC 1 cut(s) 683
BstENI CCTNNNNNAGG 1 cut(s) 647
BstKTI GATC 3 cut(s) 185, 196, 550
BstMBI GATC 3 cut(s) 182, 193, 547
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 3 cut(s) 62, 145, 414
BstPI GGTNACC 1 cut(s) 683
BstSCI CCNGG 3 cut(s) 60, 143, 412
BstSNI TACGTA 1 cut(s) 435
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 2 cut(s) 182, 547
BstYI RGATCY 2 cut(s) 182, 547
BstZ17I GTATAC 1 cut(s) 432
Bsu36I CCTNAGG 1 cut(s) 27
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 5 cut(s) 66, 143, 417, 523, 807
BtrI CACGTC 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 545
BtsIMutI CAGTG 2 cut(s) 331, 545
CaiI CAGNNNCTG 1 cut(s) 233
Cfr10I RCCGGY 2 cut(s) 318, 442
Cfr13I GGNCC 3 cut(s) 64, 316, 416
CseI GACGC 2 cut(s) 371, 799
Csp6I GTAC 2 cut(s) 506, 591
CviAII CATG 5 cut(s) 215, 311, 380, 450, 588
CviQI GTAC 2 cut(s) 506, 591
DdeI CTNAG 6 cut(s) 27, 77, 186, 258, 266, 485
DpnI GATC 3 cut(s) 184, 195, 549
DpnII GATC 3 cut(s) 182, 193, 547
EaeI YGGCCR 1 cut(s) 521
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco105I TACGTA 1 cut(s) 435
Eco130I CCWWGG 3 cut(s) 138, 303, 368
Eco147I AGGCCT 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 764
Eco32I GATATC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 316
Eco81I CCTNAGG 1 cut(s) 27
Eco91I GGTNACC 1 cut(s) 683
EcoNI CCTNNNNNAGG 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 416
EcoO65I GGTNACC 1 cut(s) 683
EcoRII CCWGG 3 cut(s) 60, 143, 412
EcoRV GATATC 1 cut(s) 99
EcoT14I CCWWGG 3 cut(s) 138, 303, 368
EcoT38I GRGCYC 1 cut(s) 764
ErhI CCWWGG 3 cut(s) 138, 303, 368
FaeI CATG 5 cut(s) 218, 314, 383, 453, 591
FaqI GGGAC 2 cut(s) 179, 329
FatI CATG 5 cut(s) 214, 310, 379, 449, 587
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 2 cut(s) 5, 8
FriOI GRGCYC 1 cut(s) 764
Fsp4HI GCNGC 2 cut(s) 5, 8
FspBI CTAG 2 cut(s) 276, 803
GluI GCNGC 2 cut(s) 5, 8
HaeIII GGCC 5 cut(s) 66, 143, 417, 523, 807
HapII CCGG 2 cut(s) 319, 443
HgaI GACGC 2 cut(s) 371, 799
Hin1I GRCGYC 1 cut(s) 791
Hin1II CATG 5 cut(s) 218, 314, 383, 453, 591
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 4 cut(s) 167, 268, 724, 755
HpaII CCGG 2 cut(s) 319, 443
HphI GGTGA 2 cut(s) 682, 689
Hpy166II GTNNAC 4 cut(s) 329, 432, 506, 543
Hpy188I TCNGA 5 cut(s) 126, 187, 259, 267, 777
Hpy188III TCNNGA 1 cut(s) 171
Hpy8I GTNNAC 4 cut(s) 329, 432, 506, 543
Hpy99I CGWCG 2 cut(s) 365, 777
HpyAV CCTTC 1 cut(s) 354
HpyCH4III ACNGT 3 cut(s) 326, 584, 595
HpyCH4IV ACGT 2 cut(s) 110, 434
HpyCH4V TGCA 2 cut(s) 227, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 6 cut(s) 27, 77, 186, 258, 266, 485
HpySE526I ACGT 2 cut(s) 110, 434
Hsp92I GRCGYC 1 cut(s) 791
Hsp92II CATG 5 cut(s) 218, 314, 383, 453, 591
Kzo9I GATC 3 cut(s) 182, 193, 547
LguI GCTCTTC 1 cut(s) 42
LmnI GCTCC 2 cut(s) 230, 370
LweI GCATC 1 cut(s) 744
MaeI CTAG 2 cut(s) 276, 803
MaeII ACGT 2 cut(s) 110, 434
MaeIII GTNAC 2 cut(s) 278, 683
MalI GATC 3 cut(s) 184, 195, 549
MboI GATC 3 cut(s) 182, 193, 547
MboII GAAGA 4 cut(s) 59, 105, 108, 542
MflI RGATCY 2 cut(s) 182, 547
MhlI GDGCHC 1 cut(s) 764
MluCI AATT 2 cut(s) 555, 689
MlyI GAGTC 2 cut(s) 161, 277
MmeI TCCRAC 2 cut(s) 235, 755
MnlI CCTC 9 cut(s) 22, 31, 181, 273, 429, 477, 643, 673, 712
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 2 cut(s) 246, 729
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 2 cut(s) 319, 443
MspR9I CCNGG 3 cut(s) 62, 145, 414
MvaI CCWGG 3 cut(s) 62, 145, 414
MwoI GCNNNNNNNGC 1 cut(s) 305
NdeII GATC 3 cut(s) 182, 193, 547
NlaIII CATG 5 cut(s) 218, 314, 383, 453, 591
NlaIV GGNNCC 1 cut(s) 317
NmeAIII GCCGAG 1 cut(s) 549
NmuCI GTSAC 1 cut(s) 278
PceI AGGCCT 1 cut(s) 143
PciSI GCTCTTC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 2 cut(s) 724, 755
PflMI CCANNNNNTGG 1 cut(s) 144
PkrI GCNGC 2 cut(s) 6, 9
PleI GAGTC 2 cut(s) 161, 276
PpsI GAGTC 2 cut(s) 161, 276
Ppu21I YACGTR 1 cut(s) 435
PsiI TTATAA 1 cut(s) 500
Psp6I CCWGG 3 cut(s) 60, 143, 412
PspEI GGTNACC 1 cut(s) 683
PspGI CCWGG 3 cut(s) 60, 143, 412
PspN4I GGNNCC 1 cut(s) 317
PspPI GGNCC 3 cut(s) 64, 316, 416
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 2 cut(s) 182, 547
RsaI GTAC 2 cut(s) 507, 592
RsaNI GTAC 2 cut(s) 506, 591
SapI GCTCTTC 1 cut(s) 42
SaqAI TTAA 2 cut(s) 246, 729
SatI GCNGC 2 cut(s) 5, 8
Sau3AI GATC 3 cut(s) 182, 193, 547
Sau96I GGNCC 3 cut(s) 64, 316, 416
SchI GAGTC 2 cut(s) 161, 277
ScrFI CCNGG 3 cut(s) 62, 145, 414
SduI GDGCHC 1 cut(s) 764
SfaNI GCATC 1 cut(s) 744
SinI GGWCC 1 cut(s) 316
SmlI CTYRAG 2 cut(s) 656, 728
SmoI CTYRAG 2 cut(s) 656, 728
SnaBI TACGTA 1 cut(s) 435
SpeI ACTAGT 1 cut(s) 275
Sse9I AATT 2 cut(s) 555, 689
SseBI AGGCCT 1 cut(s) 143
SspMI CTAG 2 cut(s) 276, 803
StuI AGGCCT 1 cut(s) 143
StyD4I CCNGG 3 cut(s) 60, 143, 412
StyI CCWWGG 3 cut(s) 138, 303, 368
TaaI ACNGT 3 cut(s) 326, 584, 595
TaiI ACGT 2 cut(s) 113, 437
TaqI TCGA 4 cut(s) 271, 347, 510, 758
TasI AATT 2 cut(s) 555, 689
TatI WGTACW 1 cut(s) 505
TfiI GAWTC 2 cut(s) 724, 755
Tru1I TTAA 2 cut(s) 246, 729
Tru9I TTAA 2 cut(s) 246, 729
TscAI CASTG 2 cut(s) 331, 545
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 2 cut(s) 4, 7
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 5 cut(s) 576, 582, 591, 644, 735
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 2 cut(s) 331, 545
Van91I CCANNNNNTGG 1 cut(s) 144
Vha464I CTTAAG 1 cut(s) 728
VpaK11BI GGWCC 1 cut(s) 316
XagI CCTNNNNNAGG 1 cut(s) 647
XapI RAATTY 1 cut(s) 555
XmiI GTMKAC 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 2 cut(s) 276, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.