Rh3AG213900

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0009701
Physical Location & Seq
Reverse (-)
21571133 .. 21571822
690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG213900.1

Sequence Viewer

Length: 540 bp
ATGTTCTCAACCAGCGCTAGTCCTTGGACGACAAATGGCATCTGGGCAGTCAACCTTGCGGGGGAATCTCCTCCAGAGTATAAGTATGTGGTACTTGATAACTGTCCATCAGCCCCTCCATTTGACCATCAGCTTGCTGACGGTGTTATAAGTGCAATCTCTAAAGCTTGGCCTAATCCTTTTTGGAATATCACGACAGCCACGGAAATTTGGAGACATAAATATGAGATACACGGTTCATGTACCATAGATGTATTACCAAGTTCCACTAGTTATTTACTGCAAGCACTTTACTTGTGGGAGAAACTCAAGTTTCACGAGTTGATGGGTGAGAATGGCAATTATAAGCCAAATACTCAATATCAAACTGAGGACGTGCTGAACGGCATCAAACGTTTCTATTATGTCCAACCAATATTGATATGTTCTGGTAGTGAATTAGTGGATGTTAGGTTTTGTCATACAGGACATTGGGTACTAATAAATTGCATGGACACCCAATCAACTTGTACTGGTCTTATTCAATATAGTCGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

179

Amino Acids

20.33

Weight (kDa)

5.6

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 6 - 163 1.5e-16 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 149, 345
AciI CCGC 1 cut(s) 59
AclI AACGTT 1 cut(s) 394
AcsI RAATTY 1 cut(s) 207
AfaI GTAC 4 cut(s) 93, 244, 477, 511
AfeI AGCGCT 1 cut(s) 16
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 1 cut(s) 524
AhlI ACTAGT 1 cut(s) 269
AjiI CACGTC 1 cut(s) 376
AluBI AGCT 3 cut(s) 133, 167, 537
AluI AGCT 3 cut(s) 133, 167, 537
Alw26I GTCTC 1 cut(s) 208
Aor51HI AGCGCT 1 cut(s) 16
AoxI GGCC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 207
AspLEI GCGC 1 cut(s) 17
AsuHPI GGTGA 1 cut(s) 341
BauI CACGAG 1 cut(s) 317
BccI CCATC 3 cut(s) 115, 135, 319
BceAI ACGGC 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 269
BfaI CTAG 3 cut(s) 18, 270, 538
BfoI RGCGCY 1 cut(s) 18
BmgBI CACGTC 1 cut(s) 376
BmsI GCATC 2 cut(s) 48, 396
BpmI CTGGAG 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 23, 201
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 517
BseDI CCNNGG 2 cut(s) 23, 201
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 1 cut(s) 60
BshFI GGCC 1 cut(s) 172
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 208
BsnI GGCC 1 cut(s) 172
BspACI CCGC 1 cut(s) 59
BspANI GGCC 1 cut(s) 172
BspCNI CTCAG 1 cut(s) 361
BsrI ACTGG 1 cut(s) 517
BssECI CCNNGG 2 cut(s) 23, 201
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 317
Bst4CI ACNGT 3 cut(s) 104, 143, 236
BstC8I GCNNGC 2 cut(s) 135, 285
BstDEI CTNAG 1 cut(s) 369
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 451
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 1 cut(s) 17
BstMAI GTCTC 1 cut(s) 208
BsuRI GGCC 1 cut(s) 172
BtgI CCRYGG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 1 cut(s) 451
Cac8I GCNNGC 2 cut(s) 135, 285
CfoI GCGC 1 cut(s) 17
Csp6I GTAC 4 cut(s) 92, 243, 476, 510
CviAII CATG 2 cut(s) 240, 490
CviJI RGCY 7 cut(s) 113, 133, 167, 172, 200, 349, 537
CviKI_1 RGCY 7 cut(s) 113, 133, 167, 172, 200, 349, 537
CviQI GTAC 4 cut(s) 92, 243, 476, 510
DdeI CTNAG 1 cut(s) 369
Eco130I CCWWGG 1 cut(s) 23
Eco47III AGCGCT 1 cut(s) 16
EcoT14I CCWWGG 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 2 cut(s) 243, 493
FatI CATG 2 cut(s) 239, 489
FauI CCCGC 1 cut(s) 52
FokI GGATG 1 cut(s) 458
FspBI CTAG 3 cut(s) 18, 270, 538
GlaI GCGC 1 cut(s) 16
GsuI CTGGAG 1 cut(s) 57
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 1 cut(s) 172
HhaI GCGC 1 cut(s) 17
Hin1II CATG 2 cut(s) 243, 493
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 165
HinfI GANTC 1 cut(s) 65
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 534
Hpy188III TCNNGA 3 cut(s) 74, 193, 317
Hpy8I GTNNAC 1 cut(s) 52
HpyCH4III ACNGT 3 cut(s) 104, 143, 236
HpyCH4IV ACGT 2 cut(s) 375, 394
HpyCH4V TGCA 3 cut(s) 155, 283, 489
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 2 cut(s) 375, 394
Hsp92II CATG 2 cut(s) 243, 493
HspAI GCGC 1 cut(s) 15
LmnI GCTCC 1 cut(s) 534
LpnPI CCDG 6 cut(s) 25, 28, 87, 414, 450, 498
LweI GCATC 2 cut(s) 48, 396
MaeI CTAG 3 cut(s) 18, 270, 538
MaeII ACGT 2 cut(s) 375, 394
MluCI AATT 4 cut(s) 207, 340, 437, 484
MmeI TCCRAC 2 cut(s) 433, 512
MnlI CCTC 3 cut(s) 81, 126, 364
MslI CAYNNNNRTG 1 cut(s) 222
NlaIII CATG 2 cut(s) 243, 493
PfeI GAWTC 1 cut(s) 65
PsiI TTATAA 2 cut(s) 149, 345
Psp1406I AACGTT 1 cut(s) 394
RsaI GTAC 4 cut(s) 93, 244, 477, 511
RsaNI GTAC 4 cut(s) 92, 243, 476, 510
RseI CAYNNNNRTG 1 cut(s) 222
SetI ASST 7 cut(s) 57, 135, 169, 378, 397, 455, 539
SfaNI GCATC 2 cut(s) 48, 396
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SpeI ACTAGT 1 cut(s) 269
Sse9I AATT 4 cut(s) 207, 340, 437, 484
SsiI CCGC 1 cut(s) 59
SspI AATATT 1 cut(s) 417
SspMI CTAG 3 cut(s) 18, 270, 538
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 3 cut(s) 104, 143, 236
TaiI ACGT 2 cut(s) 378, 397
TasI AATT 4 cut(s) 207, 340, 437, 484
TatI WGTACW 1 cut(s) 509
TfiI GAWTC 1 cut(s) 65
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 1 cut(s) 218
XapI RAATTY 1 cut(s) 207
XspI CTAG 3 cut(s) 18, 270, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.