RchiOBHm_Chr4g0425881
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008860
Physical Location & Seq
Forward (+)
51468977 .. 51470842
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39498

Sequence Viewer

Length: 942 bp
ATGCCTCATTCTCTGCTCTTTCTCGCCAGCACTGCCGGAGACTATGCATTCGTCATGTTGAGGCTTCGTGACGGCCTGAAGCATAAGCCTAGTGGCTGGTCAGCCAGTGACCACTGCATGTGGGATGGGGTGGAGTGTGACTCTAATCATAGAGTTGTGTCCATCAACATGGCCTCTAAGGCTCTGTCCGGCTCTCTCCCTCCAAGCCTAAACGCCTTGATGAAGGTCACTAGGGGCGGATCCAAGCCTTTCTTGGTAGAGCTCAAGCCATACCAAGAATTACTCTTTGTTTTGGGTTCAAAAAAATCCAGCAAAAGGCCCATTCATAAATATGCCAATCAAAAGCTTGTCCGCAGAGATTTCAAGCCCGCAAACGTTCTACTTGGAGAGGACTTTACTGCCGAAGTTGCTGACTTCGGTCTTGTCCTTCTGTGTTCTTCAAGAAATAGGTCATCGTCTCCGCTTTCACAGCCAGCTAGAACTCCTGGATATTGGGCTCCAGAATGTTTGGCTGGAAGCGTAATTGATAAAGCAGATGTTTACAGCTTTGGGGTAATATTAATGGAGTTGATCACGGGAAAGAGAATTGATAAAGCAACACTAGCCAAGGCGATTGATGGGGAGATTAATTCCATGGAAGATGAGATTGTTTCTTGCTTTTGTTCTGTGGCAGAACTTGCCATCCACTGCACCGAAAGGGAACTCAACAGGAGGCCGCACATGTCACATGCTGTGATGGAACTTTCAAAACTAGCTGAGAAACCGTGGAAACCATCTGAGCCAATAGTTGACGACAATCCTTTGATTGCTAAGTCTATGCTTGAAGCCGTACGTGAGTGGAATGAGGAGGAGGAGGAGGAAGTAAGTACTTTGGAGTCTCGTACTACACGAAAGGCCGGTGGAGGGAAAGATGAGGGGAAAATTCGTGAAGAGGATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

313

Amino Acids

34.63

Weight (kDa)

6.11

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 112 - 247 3.3e-15 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 113 - 207 6.2e-15 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 237, 352, 369, 461, 716
AclI AACGTT 1 cut(s) 375
AclWI GGATC 2 cut(s) 234, 247
AcsI RAATTY 1 cut(s) 921
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 3 cut(s) 831, 868, 883
AfiI CCNNNNNNNGG 2 cut(s) 315, 903
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 5 cut(s) 300, 364, 441, 747, 824
AjnI CCWGG 1 cut(s) 484
AloI GAACNNNNNNTCC 2 cut(s) 666, 698
AluBI AGCT 5 cut(s) 262, 346, 476, 546, 755
AluI AGCT 5 cut(s) 262, 346, 476, 546, 755
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 3 cut(s) 33, 462, 882
AlwI GGATC 2 cut(s) 234, 247
AoxI GGCC 5 cut(s) 73, 171, 317, 713, 894
ApoI RAATTY 1 cut(s) 921
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AseI ATTAAT 2 cut(s) 560, 627
AspS9I GGNCC 1 cut(s) 318
BamHI GGATCC 1 cut(s) 239
BanII GRGCYC 2 cut(s) 264, 499
Bbv12I GWGCWC 1 cut(s) 264
BccI CCATC 7 cut(s) 119, 170, 611, 689, 730, 781, 929
BceAI ACGGC 2 cut(s) 88, 812
BciT130I CCWGG 1 cut(s) 486
BclI TGATCA 1 cut(s) 570
BcoDI GTCTC 3 cut(s) 33, 462, 882
BfaI CTAG 5 cut(s) 90, 231, 477, 602, 752
BglI GCCNNNNNGGC 1 cut(s) 179
BisI GCNGC 1 cut(s) 716
BlsI GCNGC 1 cut(s) 717
BmcAI AGTACT 1 cut(s) 868
Bme1390I CCNGG 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 318
BmiI GGNNCC 2 cut(s) 241, 498
BmrFI CCNGG 1 cut(s) 486
BoxI GACNNNNGTC 1 cut(s) 417
BplI GAGNNNNNCTC 2 cut(s) 125, 157
BpmI CTGGAG 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 833
BsaJI CCNNGG 3 cut(s) 606, 633, 764
Bsc4I CCNNNNNNNGG 2 cut(s) 315, 903
Bse118I RCCGGY 1 cut(s) 896
Bse1I ACTGG 1 cut(s) 105
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 3 cut(s) 606, 633, 764
BseGI GGATG 3 cut(s) 130, 681, 940
BseLI CCNNNNNNNGG 2 cut(s) 315, 903
BseMII CTCAG 2 cut(s) 747, 768
BseNI ACTGG 1 cut(s) 105
BseRI GAGGAG 4 cut(s) 860, 863, 866, 869
BsgI GTGCAG 1 cut(s) 673
BshFI GGCC 5 cut(s) 75, 173, 319, 715, 896
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 3 cut(s) 36, 189, 897
BsiWI CGTACG 1 cut(s) 829
BslI CCNNNNNNNGG 2 cut(s) 315, 903
BsmAI GTCTC 3 cut(s) 33, 462, 882
BsmBI CGTCTC 1 cut(s) 462
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 5 cut(s) 75, 173, 319, 715, 896
Bsp1286I GDGCHC 2 cut(s) 264, 499
Bsp143I GATC 2 cut(s) 239, 570
Bsp19I CCATGG 1 cut(s) 633
BspACI CCGC 5 cut(s) 237, 352, 369, 461, 716
BspANI GGCC 5 cut(s) 75, 173, 319, 715, 896
BspCNI CTCAG 2 cut(s) 748, 769
BspLI GGNNCC 2 cut(s) 241, 498
BspPI GGATC 2 cut(s) 234, 247
BsrFI RCCGGY 1 cut(s) 896
BsrI ACTGG 1 cut(s) 105
BssAI RCCGGY 1 cut(s) 896
BssECI CCNNGG 3 cut(s) 606, 633, 764
BssMI GATC 2 cut(s) 239, 570
BssT1I CCWWGG 2 cut(s) 606, 633
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 1 cut(s) 765
Bst6I CTCTTC 1 cut(s) 924
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBAI YACGTR 1 cut(s) 833
BstC8I GCNNGC 3 cut(s) 28, 369, 474
BstDEI CTNAG 4 cut(s) 177, 756, 777, 810
BstDSI CCRYGG 2 cut(s) 633, 764
BstF5I GGATG 3 cut(s) 130, 681, 940
BstKTI GATC 2 cut(s) 242, 573
BstMAI GTCTC 3 cut(s) 33, 462, 882
BstMBI GATC 2 cut(s) 239, 570
BstMWI GCNNNNNNNGC 6 cut(s) 32, 179, 407, 469, 602, 677
BstNI CCWGG 1 cut(s) 486
BstNSI RCATGY 3 cut(s) 121, 724, 731
BstPAI GACNNNNGTC 1 cut(s) 417
BstSCI CCNGG 1 cut(s) 484
BstX2I RGATCY 1 cut(s) 239
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 1 cut(s) 239
BsuRI GGCC 5 cut(s) 75, 173, 319, 715, 896
BtgI CCRYGG 2 cut(s) 633, 764
BtsCI GGATG 3 cut(s) 130, 681, 940
BtsI GCAGTG 3 cut(s) 30, 112, 685
BtsIMutI CAGTG 4 cut(s) 30, 112, 112, 685
Cac8I GCNNGC 3 cut(s) 28, 369, 474
Cfr10I RCCGGY 1 cut(s) 896
Cfr13I GGNCC 1 cut(s) 318
Csp6I GTAC 3 cut(s) 830, 867, 882
CviAII CATG 6 cut(s) 55, 118, 169, 634, 721, 728
CviQI GTAC 3 cut(s) 830, 867, 882
DdeI CTNAG 4 cut(s) 177, 756, 777, 810
DpnI GATC 2 cut(s) 241, 572
DpnII GATC 2 cut(s) 239, 570
Eam1104I CTCTTC 1 cut(s) 924
EarI CTCTTC 1 cut(s) 924
EciI GGCGGA 1 cut(s) 252
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 2 cut(s) 606, 633
Eco24I GRGCYC 2 cut(s) 264, 499
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 484
EcoT14I CCWWGG 2 cut(s) 606, 633
EcoT22I ATGCAT 1 cut(s) 49
EcoT38I GRGCYC 2 cut(s) 264, 499
ErhI CCWWGG 2 cut(s) 606, 633
Esp3I CGTCTC 1 cut(s) 462
FaeI CATG 6 cut(s) 58, 121, 172, 637, 724, 731
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FatI CATG 6 cut(s) 54, 117, 168, 633, 720, 727
FauI CCCGC 1 cut(s) 376
FbaI TGATCA 1 cut(s) 570
Fnu4HI GCNGC 1 cut(s) 716
FokI GGATG 2 cut(s) 137, 668
FriOI GRGCYC 2 cut(s) 264, 499
Fsp4HI GCNGC 1 cut(s) 716
FspBI CTAG 5 cut(s) 90, 231, 477, 602, 752
GluI GCNGC 1 cut(s) 716
GsuI CTGGAG 1 cut(s) 483
HaeIII GGCC 5 cut(s) 75, 173, 319, 715, 896
HapII CCGG 3 cut(s) 36, 189, 897
Hin1II CATG 6 cut(s) 58, 121, 172, 637, 724, 731
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 2 cut(s) 140, 875
HpaII CCGG 3 cut(s) 36, 189, 897
Hpy166II GTNNAC 2 cut(s) 541, 790
Hpy188I TCNGA 1 cut(s) 778
Hpy188III TCNNGA 4 cut(s) 68, 441, 500, 926
Hpy8I GTNNAC 2 cut(s) 541, 790
HpyAV CCTTC 2 cut(s) 217, 437
HpyCH4III ACNGT 1 cut(s) 765
HpyCH4IV ACGT 2 cut(s) 375, 832
HpyCH4V TGCA 3 cut(s) 47, 117, 690
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 179, 407, 469, 602, 677
HpyF3I CTNAG 4 cut(s) 177, 756, 777, 810
HpySE526I ACGT 2 cut(s) 375, 832
Hsp92II CATG 6 cut(s) 58, 121, 172, 637, 724, 731
Ksp22I TGATCA 1 cut(s) 570
Kzo9I GATC 2 cut(s) 239, 570
LmnI GCTCC 1 cut(s) 502
MaeI CTAG 5 cut(s) 90, 231, 477, 602, 752
MaeII ACGT 2 cut(s) 375, 832
MaeIII GTNAC 5 cut(s) 68, 107, 137, 226, 723
MalI GATC 2 cut(s) 241, 572
MboI GATC 2 cut(s) 239, 570
MboII GAAGA 3 cut(s) 429, 650, 941
MflI RGATCY 1 cut(s) 239
MhlI GDGCHC 2 cut(s) 264, 499
MluCI AATT 5 cut(s) 278, 522, 585, 628, 921
MlyI GAGTC 2 cut(s) 134, 884
Mph1103I ATGCAT 1 cut(s) 49
MseI TTAA 2 cut(s) 560, 627
MslI CAYNNNNRTG 2 cut(s) 167, 330
MspI CCGG 3 cut(s) 36, 189, 897
MspR9I CCNGG 1 cut(s) 486
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 1 cut(s) 486
MwoI GCNNNNNNNGC 6 cut(s) 32, 179, 407, 469, 602, 677
NcoI CCATGG 1 cut(s) 633
NdeII GATC 2 cut(s) 239, 570
NlaIII CATG 6 cut(s) 58, 121, 172, 637, 724, 731
NlaIV GGNNCC 2 cut(s) 241, 498
NmuCI GTSAC 5 cut(s) 68, 107, 137, 226, 723
NsiI ATGCAT 1 cut(s) 49
NspI RCATGY 3 cut(s) 121, 724, 731
PciI ACATGT 1 cut(s) 720
PcsI WCGNNNNNNNCGW 1 cut(s) 886
PctI GAATGC 1 cut(s) 47
Pfl23II CGTACG 1 cut(s) 829
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 1 cut(s) 717
PleI GAGTC 2 cut(s) 134, 883
PpsI GAGTC 2 cut(s) 134, 883
Ppu21I YACGTR 1 cut(s) 833
PscI ACATGT 1 cut(s) 720
PshAI GACNNNNGTC 1 cut(s) 417
PshBI ATTAAT 2 cut(s) 560, 627
Psp124BI GAGCTC 1 cut(s) 264
Psp1406I AACGTT 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 484
PspGI CCWGG 1 cut(s) 484
PspLI CGTACG 1 cut(s) 829
PspN4I GGNNCC 2 cut(s) 241, 498
PspPI GGNCC 1 cut(s) 318
PsuI RGATCY 1 cut(s) 239
RsaI GTAC 3 cut(s) 831, 868, 883
RsaNI GTAC 3 cut(s) 830, 867, 882
RseI CAYNNNNRTG 2 cut(s) 167, 330
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 2 cut(s) 560, 627
SatI GCNGC 1 cut(s) 716
Sau3AI GATC 2 cut(s) 239, 570
Sau96I GGNCC 1 cut(s) 318
ScaI AGTACT 1 cut(s) 868
SchI GAGTC 2 cut(s) 134, 884
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 2 cut(s) 264, 499
SetI ASST 9 cut(s) 228, 264, 348, 378, 452, 478, 548, 757, 835
SmiMI CAYNNNNRTG 2 cut(s) 167, 330
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 5 cut(s) 278, 522, 585, 628, 921
SsiI CCGC 5 cut(s) 237, 352, 369, 461, 716
SspI AATATT 1 cut(s) 558
SspMI CTAG 5 cut(s) 90, 231, 477, 602, 752
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 484
StyI CCWWGG 2 cut(s) 606, 633
TaaI ACNGT 1 cut(s) 765
TaiI ACGT 2 cut(s) 378, 835
TaqII GACCGA 1 cut(s) 407
TasI AATT 5 cut(s) 278, 522, 585, 628, 921
TatI WGTACW 1 cut(s) 866
TauI GCSGC 1 cut(s) 718
Tru1I TTAA 2 cut(s) 560, 627
Tru9I TTAA 2 cut(s) 560, 627
TscAI CASTG 4 cut(s) 37, 112, 119, 692
TseFI GTSAC 5 cut(s) 68, 107, 137, 226, 723
Tsp45I GTSAC 5 cut(s) 68, 107, 137, 226, 723
TspDTI ATGAA 2 cut(s) 236, 314
TspRI CASTG 4 cut(s) 37, 112, 119, 692
VspI ATTAAT 2 cut(s) 560, 627
XapI RAATTY 1 cut(s) 921
XceI RCATGY 3 cut(s) 121, 724, 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XspI CTAG 5 cut(s) 90, 231, 477, 602, 752
ZrmI AGTACT 1 cut(s) 868
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.