Rh4DG266500

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008860
Physical Location & Seq
Forward (+)
48583738 .. 48608100
24363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG266500.1

Sequence Viewer

Length: 1146 bp
ATGTCTCATTCTCTGCTCTTGCTCGCCAGCACTGTCGGAGACTATGCATCCGTCATGTTGAGGCTTCGTGACGGCCTGAAGCACAAGCCTAGTGGCTGGTCAACTAGTGACCACTGCATGTGGGATGGGTTGGAGTGTGACTCTAATCATAGAGTTGTGTCCATCAACATGGCCTCTAAGGCTCTGTCCGACTCTCTCCCTTCTGTCCGACTCTCTCACTCCAAGCCTAAACGCCCTGATGAAGAACTTGCCATCCATTGCACTGAAAGGGAATCCAACAGGAGGCCGCACATGTCACATGCTGTGATGGAACTTTCAAAATTAGCTGAGAAACCGTGGAAACCATCAGAGCCAATAGTTGATGACAATCCTTTGATTGCTAAGTCTATGCTTGAAGCCGTACATGAGTGGAAGGAGGATGAGGAGGAAACTGGTAACAAAGTTGATGAAGTACTTGATGAGACTCTTATGCTTGAGGTTCATGCCAGTGGGGAAAGGCATACACCTGATGAAAGTTCTGCTGTTATGGATACTTCTGAGGGTCTCGTTAACACTCTGAGTCTTGCAAATGTGACTGTGAGGAGTGTAGATACTTTTTACTTCAGCAAGCTTTCTCAAGCAAGCTCGGATTTTTTTCTTGAACGTTCTGCTGGTGTGGATGCTAGATCCAAGAAGCTTCTGGATGCACTGGTTGAGATTGTTATAGAGGACTTCCACACTGTGCCTGAAGAGAGGGACCAAATTGCTAAACTTGTTTCAGCAATGCCAACTTACCGACGAGCTGTGAGCACACAGGAAGGGGAGCAATTTGCAAAGGAACATGGATTGATCTTCATGGAGGCCTCTGCTAAAACTGCTCAGAATGTTGAGGAGGCATTCATAAAAACTGCTGCAACCATATACAAGAAGATTCAAGATGGCGTTTTTGATGTATTAAATGAGGTACTACTAATTGTGCGTTTTCTCCAGCATGAGTATAATCTTGGCCGACCTTTCAACCAAGTTCTGAAAACCCCAACTACTCTCATGTCCATTGATAGAAGTCCAAGGATTAAACTTTTCATTGCCTGGAAATACCAGATGTTTCCAGCACAATGGATTGAGGGTCGCTTTGTGCCACGACTTGCACACAAAAGGGACATCTAA

Protein Analysis

381

Amino Acids

43.14

Weight (kDa)

5.71

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 259 - 301 9.4e-09 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 287
AclI AACGTT 1 cut(s) 643
AclWI GGATC 1 cut(s) 660
AcoI YGGCCR 1 cut(s) 985
AcuI CTGAAG 3 cut(s) 98, 586, 747
AdeI CACNNNGTG 1 cut(s) 721
AfaI GTAC 3 cut(s) 402, 453, 945
AfiI CCNNNNNNNGG 1 cut(s) 282
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 5 cut(s) 318, 395, 641, 914, 997
AhlI ACTAGT 1 cut(s) 104
AjnI CCWGG 1 cut(s) 1067
AloI GAACNNNNNNTCC 2 cut(s) 237, 269
AluBI AGCT 5 cut(s) 326, 610, 624, 676, 782
AluI AGCT 5 cut(s) 326, 610, 624, 676, 782
Alw21I GWGCWC 1 cut(s) 791
Alw26I GTCTC 4 cut(s) 9, 33, 455, 548
AlwI GGATC 1 cut(s) 660
AoxI GGCC 5 cut(s) 73, 171, 284, 840, 985
ApeKI GCWGC 1 cut(s) 890
AspS9I GGNCC 1 cut(s) 736
AvaII GGWCC 1 cut(s) 736
Bbv12I GWGCWC 1 cut(s) 791
BbvI GCAGC 1 cut(s) 877
BccI CCATC 6 cut(s) 119, 170, 260, 301, 352, 911
BceAI ACGGC 2 cut(s) 88, 383
BciT130I CCWGG 1 cut(s) 1069
BciVI GTATCC 1 cut(s) 523
BcoDI GTCTC 4 cut(s) 9, 33, 455, 548
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 3 cut(s) 90, 105, 663
BfuI GTATCC 1 cut(s) 523
BglI GCCNNNNNGGC 1 cut(s) 179
BisI GCNGC 2 cut(s) 287, 891
BlsI GCNGC 2 cut(s) 288, 892
BmcAI AGTACT 1 cut(s) 453
Bme1390I CCNGG 1 cut(s) 1069
Bme18I GGWCC 1 cut(s) 736
BmgT120I GGNCC 1 cut(s) 736
BmiI GGNNCC 1 cut(s) 737
BmrFI CCNGG 1 cut(s) 1069
BmsI GCATC 3 cut(s) 56, 649, 673
BplI GAGNNNNNCTC 2 cut(s) 125, 157
BpmI CTGGAG 1 cut(s) 950
BpuEI CTTGAG 2 cut(s) 494, 600
BsaBI GATNNNNATC 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 2 cut(s) 335, 1046
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse1I ACTGG 3 cut(s) 436, 486, 693
Bse3DI GCAATG 3 cut(s) 256, 768, 1062
Bse8I GATNNNNATC 1 cut(s) 366
BseBI CCWGG 1 cut(s) 1069
BseDI CCNNGG 2 cut(s) 335, 1046
BseGI GGATG 6 cut(s) 47, 130, 252, 424, 664, 688
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMI GCAATG 3 cut(s) 256, 768, 1062
BseMII CTCAG 4 cut(s) 318, 528, 548, 872
BseNI ACTGG 3 cut(s) 436, 486, 693
BseRI GAGGAG 3 cut(s) 437, 595, 884
BseXI GCAGC 1 cut(s) 877
BshFI GGCC 5 cut(s) 75, 173, 286, 842, 987
BsiHKAI GWGCWC 1 cut(s) 791
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 4 cut(s) 9, 33, 455, 548
BsmFI GGGAC 1 cut(s) 749
BsmI GAATGC 1 cut(s) 875
BsnI GGCC 5 cut(s) 75, 173, 286, 842, 987
Bso31I GGTCTC 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 791
Bsp143I GATC 2 cut(s) 665, 828
BspACI CCGC 1 cut(s) 287
BspANI GGCC 5 cut(s) 75, 173, 286, 842, 987
BspCNI CTCAG 4 cut(s) 319, 529, 549, 871
BspLI GGNNCC 1 cut(s) 737
BspPI GGATC 1 cut(s) 660
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 3 cut(s) 256, 768, 1062
BsrI ACTGG 3 cut(s) 436, 486, 693
BssECI CCNNGG 2 cut(s) 335, 1046
BssMI GATC 2 cut(s) 665, 828
BssT1I CCWWGG 1 cut(s) 1046
Bst2UI CCWGG 1 cut(s) 1069
Bst4CI ACNGT 4 cut(s) 34, 336, 577, 721
Bst6I CTCTTC 1 cut(s) 723
BstC8I GCNNGC 4 cut(s) 24, 28, 608, 622
BstDEI CTNAG 6 cut(s) 177, 327, 381, 537, 557, 858
BstDSI CCRYGG 1 cut(s) 335
BstF5I GGATG 6 cut(s) 47, 130, 252, 424, 664, 688
BstKTI GATC 2 cut(s) 668, 831
BstMAI GTCTC 4 cut(s) 9, 33, 455, 548
BstMBI GATC 2 cut(s) 665, 828
BstMWI GCNNNNNNNGC 2 cut(s) 179, 854
BstNI CCWGG 1 cut(s) 1069
BstNSI RCATGY 3 cut(s) 121, 295, 302
BstSCI CCNGG 1 cut(s) 1067
BstV1I GCAGC 1 cut(s) 877
BstX2I RGATCY 1 cut(s) 665
BstXI CCANNNNNNTGG 2 cut(s) 169, 1095
BstYI RGATCY 1 cut(s) 665
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 5 cut(s) 75, 173, 286, 842, 987
BtgI CCRYGG 1 cut(s) 335
BtsCI GGATG 6 cut(s) 47, 130, 252, 424, 664, 688
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 6 cut(s) 30, 112, 261, 493, 686, 717
Cac8I GCNNGC 4 cut(s) 24, 28, 608, 622
Cfr13I GGNCC 1 cut(s) 736
Csp6I GTAC 3 cut(s) 401, 452, 944
CspCI CAANNNNNGTGG 2 cut(s) 73, 108
CviQI GTAC 3 cut(s) 401, 452, 944
DdeI CTNAG 6 cut(s) 177, 327, 381, 537, 557, 858
DpnI GATC 2 cut(s) 667, 830
DpnII GATC 2 cut(s) 665, 828
DraIII CACNNNGTG 1 cut(s) 721
EaeI YGGCCR 1 cut(s) 985
Eam1104I CTCTTC 1 cut(s) 723
EarI CTCTTC 1 cut(s) 723
Eco130I CCWWGG 1 cut(s) 1046
Eco147I AGGCCT 1 cut(s) 842
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 736
Eco57I CTGAAG 3 cut(s) 98, 586, 747
EcoRII CCWGG 1 cut(s) 1067
EcoT14I CCWWGG 1 cut(s) 1046
EcoT22I ATGCAT 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 1046
FaqI GGGAC 1 cut(s) 749
Fnu4HI GCNGC 2 cut(s) 287, 891
FokI GGATG 6 cut(s) 34, 137, 239, 431, 671, 695
Fsp4HI GCNGC 2 cut(s) 287, 891
FspBI CTAG 3 cut(s) 90, 105, 663
GluI GCNGC 2 cut(s) 287, 891
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 5 cut(s) 75, 173, 286, 842, 987
HincII GTYRAC 2 cut(s) 102, 550
HindII GTYRAC 2 cut(s) 102, 550
HindIII AAGCTT 2 cut(s) 608, 674
HinfI GANTC 7 cut(s) 140, 191, 210, 272, 463, 559, 910
HpaI GTTAAC 1 cut(s) 550
Hpy166II GTNNAC 2 cut(s) 102, 550
Hpy188I TCNGA 9 cut(s) 38, 190, 209, 349, 538, 558, 628, 861, 1008
Hpy188III TCNNGA 4 cut(s) 68, 638, 680, 914
Hpy8I GTNNAC 2 cut(s) 102, 550
Hpy99I CGWCG 1 cut(s) 780
HpyAV CCTTC 3 cut(s) 210, 406, 791
HpyCH4III ACNGT 4 cut(s) 34, 336, 577, 721
HpyCH4IV ACGT 1 cut(s) 643
HpyCH4V TGCA 8 cut(s) 47, 117, 261, 566, 686, 812, 893, 1127
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 854
HpyF3I CTNAG 6 cut(s) 177, 327, 381, 537, 557, 858
HpySE526I ACGT 1 cut(s) 643
KspAI GTTAAC 1 cut(s) 550
Kzo9I GATC 2 cut(s) 665, 828
LmnI GCTCC 1 cut(s) 802
Lsp1109I GCAGC 1 cut(s) 877
LweI GCATC 3 cut(s) 56, 649, 673
MaeI CTAG 3 cut(s) 90, 105, 663
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 6 cut(s) 68, 107, 137, 294, 434, 571
MalI GATC 2 cut(s) 667, 830
MboI GATC 2 cut(s) 665, 828
MboII GAAGA 4 cut(s) 254, 740, 823, 919
MflI RGATCY 1 cut(s) 665
MhlI GDGCHC 1 cut(s) 791
MluCI AATT 4 cut(s) 320, 741, 806, 951
MlyI GAGTC 5 cut(s) 134, 185, 204, 457, 568
MmeI TCCRAC 5 cut(s) 16, 111, 213, 232, 300
Mph1103I ATGCAT 1 cut(s) 49
MseI TTAA 3 cut(s) 549, 935, 1053
MslI CAYNNNNRTG 2 cut(s) 167, 486
MspR9I CCNGG 1 cut(s) 1069
Mva1269I GAATGC 1 cut(s) 875
MvaI CCWGG 1 cut(s) 1069
MwoI GCNNNNNNNGC 2 cut(s) 179, 854
NdeII GATC 2 cut(s) 665, 828
NlaIV GGNNCC 1 cut(s) 737
NmuCI GTSAC 5 cut(s) 68, 107, 137, 294, 571
NsiI ATGCAT 1 cut(s) 49
NspI RCATGY 3 cut(s) 121, 295, 302
PceI AGGCCT 1 cut(s) 842
PciI ACATGT 1 cut(s) 291
PctI GAATGC 1 cut(s) 875
PfeI GAWTC 2 cut(s) 272, 910
PkrI GCNGC 2 cut(s) 288, 892
PleI GAGTC 5 cut(s) 134, 185, 204, 457, 567
PpsI GAGTC 5 cut(s) 134, 185, 204, 457, 567
PscI ACATGT 1 cut(s) 291
Psp1406I AACGTT 1 cut(s) 643
Psp6I CCWGG 1 cut(s) 1067
PspGI CCWGG 1 cut(s) 1067
PspN4I GGNNCC 1 cut(s) 737
PspPI GGNCC 1 cut(s) 736
PsuI RGATCY 1 cut(s) 665
RsaI GTAC 3 cut(s) 402, 453, 945
RsaNI GTAC 3 cut(s) 401, 452, 944
RseI CAYNNNNRTG 2 cut(s) 167, 486
SaqAI TTAA 3 cut(s) 549, 935, 1053
SatI GCNGC 2 cut(s) 287, 891
Sau3AI GATC 2 cut(s) 665, 828
Sau96I GGNCC 1 cut(s) 736
ScaI AGTACT 1 cut(s) 453
SchI GAGTC 5 cut(s) 134, 185, 204, 457, 568
ScrFI CCNGG 1 cut(s) 1069
SduI GDGCHC 1 cut(s) 791
SfaNI GCATC 3 cut(s) 56, 649, 673
SinI GGWCC 1 cut(s) 736
SmiMI CAYNNNNRTG 2 cut(s) 167, 486
SmlI CTYRAG 2 cut(s) 473, 615
SmoI CTYRAG 2 cut(s) 473, 615
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 4 cut(s) 320, 741, 806, 951
SseBI AGGCCT 1 cut(s) 842
SsiI CCGC 1 cut(s) 287
SspMI CTAG 3 cut(s) 90, 105, 663
StuI AGGCCT 1 cut(s) 842
StyD4I CCNGG 1 cut(s) 1067
StyI CCWWGG 1 cut(s) 1046
TaaI ACNGT 4 cut(s) 34, 336, 577, 721
TaiI ACGT 1 cut(s) 646
TasI AATT 4 cut(s) 320, 741, 806, 951
TatI WGTACW 1 cut(s) 451
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 2 cut(s) 272, 910
Tru1I TTAA 3 cut(s) 549, 935, 1053
Tru9I TTAA 3 cut(s) 549, 935, 1053
TscAI CASTG 6 cut(s) 37, 119, 268, 493, 693, 724
TseFI GTSAC 5 cut(s) 68, 107, 137, 294, 571
TseI GCWGC 1 cut(s) 890
Tsp45I GTSAC 5 cut(s) 68, 107, 137, 294, 571
TspDTI ATGAA 7 cut(s) 255, 462, 470, 525, 823, 868, 1051
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 6 cut(s) 37, 119, 268, 493, 693, 724
VpaK11BI GGWCC 1 cut(s) 736
XceI RCATGY 3 cut(s) 121, 295, 302
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XspI CTAG 3 cut(s) 90, 105, 663
ZrmI AGTACT 1 cut(s) 453
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.