RchiOBHm_Chr7g0198411

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001824
Physical Location & Seq
Forward (+)
16288214 .. 16290238
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17752

Sequence Viewer

Length: 660 bp
ATGTCGTTCATTGGAACCCAGCAGAAATGCAAGGCTTGTGATAAGACTGTTCATTTCATTGAACAAGTATCTGCAGATGGTGTTCCCTATCACAAAACCTGCTTTAAATGCAGTCACTGCAATGGACAATTGTCGATGAGCAGCTACTCCGCCATGGAGGGAGTTCTATACTGCAAGCCTCATTTCGAGCAACTCTACAAGGATAGCGCTAGTAAGAAAATTCCGAGTGGGAAACCAAACGAAGTGCTGACTAAGACCCCTAGCAAATTGTCCTTTATGTTCTCTGGGACTCAAGACAAATGTGCCGTCTGCACCAAAACTGTTTATCCATTAGAGAAGGTTACGTTGGAAGGAACGTTTTACCACAAGAACTGCTTTAGGTGTAATCATGGGGGTTGCGCTCTCTCACCCTCAAACTGTGCTGCTCTAGATGGCATTCTATACTGCAAGCACCACTTTTCCCAGTTGTTCAAGGAGAAGGGCAGCTACAACCACCTCACCAAGACTGCTTCTCTGAGGAAAAATGTAGCTCCATTACCCGAGCCAAAAACCGAGGCAGCTGAGGGAGAAGAACCAAAGGCAGACGCAGGAGTGGAAGCAGGTGAAGCAGAGGCCGCAGCACAGGAGTCACAAGATGCAGCCCAGACGCAAGAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIM

Protein Analysis

219

Amino Acids

23.95

Weight (kDa)

7.51

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIM PF00412 10 - 65 8.3e-12 LIM domain
LIM PF00412 101 - 157 1.2e-09 LIM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 590
Acc36I ACCTGC 2 cut(s) 107, 590
AciI CCGC 2 cut(s) 150, 615
AclI AACGTT 1 cut(s) 356
AcsI RAATTY 1 cut(s) 219
AfeI AGCGCT 1 cut(s) 208
AgsI TTSAA 2 cut(s) 62, 472
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 4 cut(s) 144, 486, 530, 560
AluI AGCT 4 cut(s) 144, 486, 530, 560
AlwNI CAGNNNCTG 1 cut(s) 117
Ama87I CYCGRG 1 cut(s) 539
Aor51HI AGCGCT 1 cut(s) 208
AoxI GGCC 1 cut(s) 612
ApeKI GCWGC 6 cut(s) 141, 422, 483, 557, 617, 638
ApoI RAATTY 1 cut(s) 219
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
AspLEI GCGC 2 cut(s) 209, 401
AsuHPI GGTGA 3 cut(s) 399, 490, 614
AvaI CYCGRG 1 cut(s) 539
BarI GAAGNNNNNNTAC 2 cut(s) 470, 502
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 6 cut(s) 153, 409, 495, 569, 629, 650
BccI CCATC 2 cut(s) 71, 425
BceAI ACGGC 1 cut(s) 290
BfaI CTAG 3 cut(s) 210, 261, 428
BfmI CTRYAG 1 cut(s) 72
BfoI RGCGCY 1 cut(s) 210
BfuAI ACCTGC 2 cut(s) 107, 590
BisI GCNGC 7 cut(s) 142, 423, 484, 558, 615, 618, 639
BlsI GCNGC 7 cut(s) 143, 424, 485, 559, 616, 619, 640
BmeT110I CYCGRG 1 cut(s) 539
BmiI GGNNCC 1 cut(s) 16
BmrI ACTGGG 1 cut(s) 457
BmsI GCATC 1 cut(s) 625
BmuI ACTGGG 1 cut(s) 457
BoxI GACNNNNGTC 1 cut(s) 130
Bpu10I CCTNAGC 1 cut(s) 561
BpuEI CTTGAG 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 153, 552
Bse1I ACTGG 1 cut(s) 463
Bse3DI GCAATG 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 153, 552
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 2 cut(s) 506, 552
BseNI ACTGG 1 cut(s) 463
BseXI GCAGC 6 cut(s) 153, 409, 495, 569, 629, 650
BseYI CCCAGC 1 cut(s) 18
BsgI GTGCAG 1 cut(s) 295
BshFI GGCC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 539
BslFI GGGAC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 301
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 1 cut(s) 614
BsoBI CYCGRG 1 cut(s) 539
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 2 cut(s) 150, 615
BspANI GGCC 1 cut(s) 614
BspCNI CTCAG 2 cut(s) 507, 553
BspLI GGNNCC 1 cut(s) 16
BspMAI CTGCAG 1 cut(s) 76
BspMI ACCTGC 2 cut(s) 107, 590
BsrDI GCAATG 1 cut(s) 127
BsrI ACTGG 1 cut(s) 463
BssECI CCNNGG 2 cut(s) 153, 552
BssT1I CCWWGG 1 cut(s) 153
Bst4CI ACNGT 3 cut(s) 49, 322, 419
BstAPI GCANNNNNTGC 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 176, 449
BstDEI CTNAG 3 cut(s) 252, 515, 561
BstDSI CCRYGG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 2 cut(s) 209, 401
BstMWI GCNNNNNNNGC 4 cut(s) 108, 117, 605, 614
BstPAI GACNNNNGTC 1 cut(s) 130
BstSFI CTRYAG 1 cut(s) 72
BstV1I GCAGC 6 cut(s) 153, 409, 495, 569, 629, 650
BsuRI GGCC 1 cut(s) 614
BtgI CCRYGG 1 cut(s) 153
BtsI GCAGTG 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 115
BveI ACCTGC 2 cut(s) 107, 590
Cac8I GCNNGC 2 cut(s) 176, 449
CaiI CAGNNNCTG 1 cut(s) 117
CfoI GCGC 2 cut(s) 209, 401
CseI GACGC 2 cut(s) 593, 655
CviAII CATG 2 cut(s) 154, 389
CviJI RGCY 9 cut(s) 35, 144, 178, 486, 530, 544, 560, 614, 641
CviKI_1 RGCY 9 cut(s) 35, 144, 178, 486, 530, 544, 560, 614, 641
DdeI CTNAG 3 cut(s) 252, 515, 561
DraI TTTAAA 1 cut(s) 106
EciI GGCGGA 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 153
Eco47III AGCGCT 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 539
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 2 cut(s) 157, 392
FaiI YATR 5 cut(s) 155, 169, 278, 390, 442
FalI AAGNNNNNCTT 4 cut(s) 359, 391, 440, 472
FaqI GGGAC 1 cut(s) 301
FatI CATG 2 cut(s) 153, 388
Fnu4HI GCNGC 7 cut(s) 142, 423, 484, 558, 615, 618, 639
Fsp4HI GCNGC 7 cut(s) 142, 423, 484, 558, 615, 618, 639
FspBI CTAG 3 cut(s) 210, 261, 428
GlaI GCGC 2 cut(s) 208, 400
GluI GCNGC 7 cut(s) 142, 423, 484, 558, 615, 618, 639
GsaI CCCAGC 1 cut(s) 22
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 1 cut(s) 614
HgaI GACGC 2 cut(s) 593, 655
HhaI GCGC 2 cut(s) 209, 401
Hin1II CATG 2 cut(s) 157, 392
Hin6I GCGC 2 cut(s) 207, 399
HinP1I GCGC 2 cut(s) 207, 399
HinfI GANTC 2 cut(s) 289, 626
HphI GGTGA 3 cut(s) 399, 490, 614
Hpy188I TCNGA 2 cut(s) 225, 516
Hpy188III TCNNGA 2 cut(s) 293, 428
HpyAV CCTTC 3 cut(s) 331, 344, 472
HpyCH4III ACNGT 3 cut(s) 49, 322, 419
HpyCH4IV ACGT 2 cut(s) 344, 356
HpyCH4V TGCA 8 cut(s) 30, 74, 111, 120, 174, 312, 447, 638
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 117, 605, 614
HpyF3I CTNAG 3 cut(s) 252, 515, 561
HpySE526I ACGT 2 cut(s) 344, 356
Hsp92II CATG 2 cut(s) 157, 392
HspAI GCGC 2 cut(s) 207, 399
LmnI GCTCC 1 cut(s) 535
LpnPI CCDG 8 cut(s) 32, 112, 270, 476, 573, 585, 608, 656
Lsp1109I GCAGC 6 cut(s) 153, 409, 495, 569, 629, 650
LweI GCATC 1 cut(s) 625
MaeI CTAG 3 cut(s) 210, 261, 428
MaeII ACGT 2 cut(s) 344, 356
MaeIII GTNAC 3 cut(s) 113, 340, 627
MboII GAAGA 1 cut(s) 581
MfeI CAATTG 1 cut(s) 128
MluCI AATT 3 cut(s) 128, 219, 266
MlyI GAGTC 2 cut(s) 283, 635
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 8 cut(s) 151, 189, 421, 506, 510, 547, 556, 604
MseI TTAA 1 cut(s) 105
MslI CAYNNNNRTG 1 cut(s) 120
MspA1I CMGCKG 1 cut(s) 560
MunI CAATTG 1 cut(s) 128
Mva1269I GAATGC 1 cut(s) 435
MwoI GCNNNNNNNGC 4 cut(s) 108, 117, 605, 614
NcoI CCATGG 1 cut(s) 153
NlaIII CATG 2 cut(s) 157, 392
NlaIV GGNNCC 1 cut(s) 16
NmuCI GTSAC 2 cut(s) 113, 627
PaqCI CACCTGC 1 cut(s) 590
PctI GAATGC 1 cut(s) 435
PkrI GCNGC 7 cut(s) 143, 424, 485, 559, 616, 619, 640
PleI GAGTC 2 cut(s) 283, 634
PpsI GAGTC 2 cut(s) 283, 634
PshAI GACNNNNGTC 1 cut(s) 130
Psp1406I AACGTT 1 cut(s) 356
PspFI CCCAGC 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 16
PstI CTGCAG 1 cut(s) 76
PstNI CAGNNNCTG 1 cut(s) 117
PvuII CAGCTG 1 cut(s) 560
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 7 cut(s) 142, 423, 484, 558, 615, 618, 639
SchI GAGTC 2 cut(s) 283, 635
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
Sse9I AATT 3 cut(s) 128, 219, 266
SsiI CCGC 2 cut(s) 150, 615
SspMI CTAG 3 cut(s) 210, 261, 428
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 3 cut(s) 49, 322, 419
TaiI ACGT 2 cut(s) 347, 359
TaqI TCGA 2 cut(s) 134, 186
TasI AATT 3 cut(s) 128, 219, 266
TauI GCSGC 1 cut(s) 617
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 1 cut(s) 122
TseFI GTSAC 2 cut(s) 113, 627
TseI GCWGC 6 cut(s) 141, 422, 483, 557, 617, 638
Tsp45I GTSAC 2 cut(s) 113, 627
TspDTI ATGAA 2 cut(s) 41, 46
TspRI CASTG 1 cut(s) 122
XapI RAATTY 1 cut(s) 219
XbaI TCTAGA 1 cut(s) 427
XspI CTAG 3 cut(s) 210, 261, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.