RLG00000001756

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011622
Physical Location & Seq
Forward (+)
20205552 .. 20206093
542 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001756

Sequence Viewer

Length: 321 bp
ATGAGACCAAAACCAAGGAGAACCCAGTCTTTGCCTCATGTTTTGGCTTTCATTCCCGAGCAAGTGCGCCACCTCCACGCCACTCACTTGCCAAAGTTCCTCGGCCTCCGATTCACCGACACTGTTAGGCTTCTCAAAGAATCGAGCTCTGGTTTAGATCTCGTGATCAGAGTTTGGCCACAAAGGCAGAGCTTCAATGACCACGACTTCGGTCCGGTTCCATCCCAATGGAAGATGAACCAGACTACAGAATACGACGGTGATGGCGAGTGTAGTTCTTGGAATTTGTTGATATATAATCGCAATGGAATTGTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

107

Amino Acids

12.28

Weight (kDa)

9.42

Isoelectric Point (pI)

55.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 176
AcsI RAATTY 1 cut(s) 283
AgsI TTSAA 1 cut(s) 196
AluBI AGCT 2 cut(s) 147, 192
AluI AGCT 2 cut(s) 147, 192
Alw21I GWGCWC 1 cut(s) 149
Ama87I CYCGRG 1 cut(s) 56
AoxI GGCC 2 cut(s) 103, 176
ApoI RAATTY 1 cut(s) 283
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 106, 272
AvaI CYCGRG 1 cut(s) 56
AvaII GGWCC 1 cut(s) 212
BalI TGGCCA 1 cut(s) 178
BanII GRGCYC 1 cut(s) 149
BauI CACGAG 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 149
BccI CCATC 2 cut(s) 229, 257
BclI TGATCA 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 246
BglI GCCNNNNNGGC 1 cut(s) 184
BglII AGATCT 1 cut(s) 157
Bme18I GGWCC 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 219
BmrI ACTGGG 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 19
BoxI GACNNNNGTC 1 cut(s) 210
BsaJI CCNNGG 2 cut(s) 14, 100
BsaWI WCCGGW 1 cut(s) 214
Bse1I ACTGG 1 cut(s) 25
Bse3DI GCAATG 1 cut(s) 310
BseDI CCNNGG 2 cut(s) 14, 100
BseGI GGATG 1 cut(s) 221
BseMI GCAATG 1 cut(s) 310
BseNI ACTGG 1 cut(s) 25
BshFI GGCC 2 cut(s) 105, 178
BsiHKAI GWGCWC 1 cut(s) 149
BsiHKCI CYCGRG 1 cut(s) 56
BsiSI CCGG 1 cut(s) 215
BsnI GGCC 2 cut(s) 105, 178
BsoBI CYCGRG 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 2 cut(s) 157, 165
BspANI GGCC 2 cut(s) 105, 178
BspLI GGNNCC 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 1 cut(s) 25
BssECI CCNNGG 2 cut(s) 14, 100
BssMI GATC 2 cut(s) 157, 165
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 14
Bst2BI CACGAG 1 cut(s) 161
Bst4CI ACNGT 2 cut(s) 124, 260
BstF5I GGATG 1 cut(s) 221
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 2 cut(s) 160, 168
BstMBI GATC 2 cut(s) 157, 165
BstMWI GCNNNNNNNGC 1 cut(s) 184
BstPAI GACNNNNGTC 1 cut(s) 210
BstSFI CTRYAG 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 157
BstXI CCANNNNNNTGG 1 cut(s) 228
BstYI RGATCY 1 cut(s) 157
BsuRI GGCC 2 cut(s) 105, 178
BtsCI GGATG 1 cut(s) 221
BtsIMutI CAGTG 1 cut(s) 120
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 212
CpoI CGGWCCG 1 cut(s) 212
CspI CGGWCCG 1 cut(s) 212
CviAII CATG 1 cut(s) 38
CviJI RGCY 6 cut(s) 47, 105, 130, 147, 178, 192
CviKI_1 RGCY 6 cut(s) 47, 105, 130, 147, 178, 192
DpnI GATC 2 cut(s) 159, 167
DpnII GATC 2 cut(s) 157, 165
EaeI YGGCCR 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 14
Eco24I GRGCYC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 147
Eco88I CYCGRG 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 14
EcoT38I GRGCYC 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 14
FaeI CATG 1 cut(s) 41
FaiI YATR 3 cut(s) 39, 295, 297
FatI CATG 1 cut(s) 37
FbaI TGATCA 1 cut(s) 165
FokI GGATG 1 cut(s) 208
FriOI GRGCYC 1 cut(s) 149
GlaI GCGC 1 cut(s) 68
HaeIII GGCC 2 cut(s) 105, 178
HapII CCGG 1 cut(s) 215
HhaI GCGC 1 cut(s) 69
Hin1II CATG 1 cut(s) 41
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HinfI GANTC 2 cut(s) 111, 140
HpaII CCGG 1 cut(s) 215
HphI GGTGA 2 cut(s) 106, 272
Hpy188I TCNGA 2 cut(s) 110, 170
Hpy188III TCNNGA 2 cut(s) 56, 163
Hpy99I CGWCG 1 cut(s) 260
HpyCH4III ACNGT 2 cut(s) 124, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 184
Hsp92II CATG 1 cut(s) 41
HspAI GCGC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 2 cut(s) 157, 165
LpnPI CCDG 4 cut(s) 38, 135, 228, 254
MalI GATC 2 cut(s) 159, 167
MboI GATC 2 cut(s) 157, 165
MboII GAAGA 1 cut(s) 244
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 149
MlsI TGGCCA 1 cut(s) 178
MluCI AATT 2 cut(s) 283, 309
MluNI TGGCCA 1 cut(s) 178
MnlI CCTC 4 cut(s) 45, 83, 110, 116
Mox20I TGGCCA 1 cut(s) 178
MscI TGGCCA 1 cut(s) 178
MslI CAYNNNNRTG 1 cut(s) 226
Msp20I TGGCCA 1 cut(s) 178
MspI CCGG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 184
NdeII GATC 2 cut(s) 157, 165
NlaIII CATG 1 cut(s) 41
NlaIV GGNNCC 1 cut(s) 219
NmeAIII GCCGAG 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PfeI GAWTC 2 cut(s) 111, 140
PshAI GACNNNNGTC 1 cut(s) 210
Psp124BI GAGCTC 1 cut(s) 149
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 212
PsuI RGATCY 1 cut(s) 157
RseI CAYNNNNRTG 1 cut(s) 226
Rsr2I CGGWCCG 1 cut(s) 212
RsrII CGGWCCG 1 cut(s) 212
SacI GAGCTC 1 cut(s) 149
Sau3AI GATC 2 cut(s) 157, 165
Sau96I GGNCC 1 cut(s) 212
SduI GDGCHC 1 cut(s) 149
SetI ASST 3 cut(s) 75, 149, 194
SfcI CTRYAG 1 cut(s) 246
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 226
Sse9I AATT 2 cut(s) 283, 309
SstI GAGCTC 1 cut(s) 149
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 2 cut(s) 124, 260
TaqI TCGA 1 cut(s) 143
TaqII GACCGA 1 cut(s) 200
TasI AATT 2 cut(s) 283, 309
TfiI GAWTC 2 cut(s) 111, 140
TscAI CASTG 1 cut(s) 127
TspDTI ATGAA 2 cut(s) 40, 251
TspRI CASTG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.