Rh6AG458300

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011622
Physical Location & Seq
Forward (+)
64165106 .. 64165968
863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG458300.1

Sequence Viewer

Length: 504 bp
ATGGTGGATGTTAAAATGTCGAAAATAACCCTAGCCTCCCCTTTTAATTTTTTTAATTTTTTCTTTTTTGTTCTTTGCTGCAATCTTTTCTTCCTCTTGTTCCTTTACCCAAACACGGGCCTCATCATCCTAGCTCTTCTTCTTCCTCTCATCAAATCCAACCTCAAAGCTAAAGAAGACAAACAAAGCCACTCCGTGAGACCAAAACCAAGGAGAACCCAGTCTTTGCCTCATGTTTTGGCTTTCATTCCCGAGCAAGTGCGCCACCTCCACGCTACTCACTCATTGGAGTTCCTCGACCTCCGATTCATCGACATTGTTGGGCTTCTCAAAGAATCGAGCTTTGGTTCAGATCTCGTGATCAGAGTTTGGCCACAGAGGCAGAGCTTCAATGACCACGACTTCGGTTCGGTTCCATCCAAATGGGAGATGAACCAGACTACGGAATCTGACGGTGATGGCGGCGACGCTGCATGCGGTTGCGATTTTGCTGAAGCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

167

Amino Acids

18.91

Weight (kDa)

6.28

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 462, 477
AcoI YGGCCR 1 cut(s) 371
AdeI CACNNNGTG 1 cut(s) 196
AfiI CCNNNNNNNGG 3 cut(s) 115, 116, 442
AgsI TTSAA 1 cut(s) 391
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AluBI AGCT 4 cut(s) 134, 170, 342, 387
AluI AGCT 4 cut(s) 134, 170, 342, 387
Alw26I GTCTC 1 cut(s) 193
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 2 cut(s) 118, 371
ApeKI GCWGC 2 cut(s) 78, 470
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 118
AsuHPI GGTGA 1 cut(s) 467
AvaI CYCGRG 1 cut(s) 251
BalI TGGCCA 1 cut(s) 373
BauI CACGAG 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 183
BbvI GCAGC 2 cut(s) 65, 457
BccI CCATC 2 cut(s) 424, 452
BclI TGATCA 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 2 cut(s) 32, 131
BglI GCCNNNNNGGC 1 cut(s) 379
BglII AGATCT 1 cut(s) 352
BisI GCNGC 3 cut(s) 79, 463, 471
BlsI GCNGC 3 cut(s) 80, 464, 472
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 414
BmrI ACTGGG 1 cut(s) 214
BmuI ACTGGG 1 cut(s) 214
BpiI GAAGAC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 1 cut(s) 209
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 116, 442
Bse1I ACTGG 1 cut(s) 220
BseDI CCNNGG 1 cut(s) 209
BseGI GGATG 3 cut(s) 13, 126, 416
BseLI CCNNNNNNNGG 3 cut(s) 115, 116, 442
BseNI ACTGG 1 cut(s) 220
BseXI GCAGC 2 cut(s) 65, 457
BshFI GGCC 2 cut(s) 120, 373
BsiHKCI CYCGRG 1 cut(s) 251
BslI CCNNNNNNNGG 3 cut(s) 115, 116, 442
BsmAI GTCTC 1 cut(s) 193
BsnI GGCC 2 cut(s) 120, 373
Bso31I GGTCTC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 251
Bsp143I GATC 2 cut(s) 352, 360
BspACI CCGC 2 cut(s) 462, 477
BspANI GGCC 2 cut(s) 120, 373
BspLI GGNNCC 1 cut(s) 414
BspQI GCTCTTC 1 cut(s) 141
BspTNI GGTCTC 1 cut(s) 193
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 2 cut(s) 352, 360
BssSI CACGAG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 209
Bst2BI CACGAG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 455
Bst6I CTCTTC 1 cut(s) 141
BstC8I GCNNGC 2 cut(s) 475, 499
BstF5I GGATG 3 cut(s) 13, 126, 416
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 2 cut(s) 355, 363
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 2 cut(s) 352, 360
BstMWI GCNNNNNNNGC 1 cut(s) 379
BstNSI RCATGY 1 cut(s) 477
BstV1I GCAGC 2 cut(s) 65, 457
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 352
BstXI CCANNNNNNTGG 1 cut(s) 423
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 2 cut(s) 120, 373
BtsCI GGATG 3 cut(s) 13, 126, 416
Cac8I GCNNGC 2 cut(s) 475, 499
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 118
CseI GACGC 1 cut(s) 476
CviAII CATG 2 cut(s) 233, 474
DpnI GATC 2 cut(s) 354, 362
DpnII GATC 2 cut(s) 352, 360
DraIII CACNNNGTG 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 371
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 209
Eco31I GGTCTC 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 251
EcoT14I CCWWGG 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 2 cut(s) 236, 477
FaiI YATR 2 cut(s) 234, 475
FatI CATG 2 cut(s) 232, 473
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 3 cut(s) 79, 463, 471
FokI GGATG 3 cut(s) 20, 113, 403
Fsp4HI GCNGC 3 cut(s) 79, 463, 471
FspBI CTAG 2 cut(s) 32, 131
GlaI GCGC 1 cut(s) 263
GluI GCNGC 3 cut(s) 79, 463, 471
HaeIII GGCC 2 cut(s) 120, 373
HgaI GACGC 1 cut(s) 476
HhaI GCGC 1 cut(s) 264
Hin1II CATG 2 cut(s) 236, 477
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HinfI GANTC 3 cut(s) 306, 335, 446
HphI GGTGA 1 cut(s) 467
Hpy188I TCNGA 4 cut(s) 305, 352, 365, 451
Hpy188III TCNNGA 2 cut(s) 251, 358
Hpy99I CGWCG 1 cut(s) 470
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4V TGCA 2 cut(s) 81, 473
HpyF10VI GCNNNNNNNGC 1 cut(s) 379
Hsp92II CATG 2 cut(s) 236, 477
HspAI GCGC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 2 cut(s) 352, 360
LguI GCTCTTC 1 cut(s) 141
LpnPI CCDG 3 cut(s) 233, 449, 483
Lsp1109I GCAGC 2 cut(s) 65, 457
MaeI CTAG 2 cut(s) 32, 131
MalI GATC 2 cut(s) 354, 362
MboI GATC 2 cut(s) 352, 360
MboII GAAGA 5 cut(s) 82, 128, 131, 134, 188
MflI RGATCY 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 373
MluCI AATT 2 cut(s) 46, 55
MluNI TGGCCA 1 cut(s) 373
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 373
MscI TGGCCA 1 cut(s) 373
MseI TTAA 3 cut(s) 12, 45, 54
MslI CAYNNNNRTG 1 cut(s) 421
Msp20I TGGCCA 1 cut(s) 373
MwoI GCNNNNNNNGC 1 cut(s) 379
NdeII GATC 2 cut(s) 352, 360
NlaIII CATG 2 cut(s) 236, 477
NlaIV GGNNCC 1 cut(s) 414
NspI RCATGY 1 cut(s) 477
PaeI GCATGC 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 141
PfeI GAWTC 3 cut(s) 306, 335, 446
PkrI GCNGC 3 cut(s) 80, 464, 472
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 1 cut(s) 118
PsuI RGATCY 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 421
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 3 cut(s) 12, 45, 54
SatI GCNGC 3 cut(s) 79, 463, 471
Sau3AI GATC 2 cut(s) 352, 360
Sau96I GGNCC 1 cut(s) 118
SetI ASST 7 cut(s) 136, 165, 172, 270, 303, 344, 389
SmiMI CAYNNNNRTG 1 cut(s) 421
SphI GCATGC 1 cut(s) 477
Sse9I AATT 2 cut(s) 46, 55
SsiI CCGC 2 cut(s) 462, 477
SspMI CTAG 2 cut(s) 32, 131
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 1 cut(s) 455
TaqI TCGA 4 cut(s) 20, 297, 312, 338
TasI AATT 2 cut(s) 46, 55
TauI GCSGC 1 cut(s) 465
TfiI GAWTC 3 cut(s) 306, 335, 446
Tru1I TTAA 3 cut(s) 12, 45, 54
Tru9I TTAA 3 cut(s) 12, 45, 54
TseI GCWGC 2 cut(s) 78, 470
TspDTI ATGAA 3 cut(s) 235, 298, 446
TspGWI ACGGA 2 cut(s) 184, 458
XceI RCATGY 1 cut(s) 477
XspI CTAG 2 cut(s) 32, 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.