Rh2DG200800

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011622
Physical Location & Seq
Forward (+)
18482822 .. 18483235
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG200800.1

Sequence Viewer

Length: 414 bp
ATGGGCCTCATCATCCTAGCTCTTCTTCTTCCTCTTATCAAATCCAACCTCAAAGCTAAAGAAGACAAACAAAGCCACTCCATGAGACCAAAACCAAGGAGAACCCAGTCTTTGCCACATGATTTGGCTTTCATTCCCAAGCAAGTGCGCCACCTCCAAGCCACTCACTCGCCGGAGTTCCTCGGCCTCCGATTCACCGACACCGTTGGGCTTCTCAAAGAATCGAGCTCTAGTTCAGATCTCGAGATCAGAGTTTGGCCACAGAGGCAGAGCTTCAATGACCATGACTTCGGTCCGGTTCCATCCAAATGGAAGATGAACCAGACTACGGAATCCGACGGTGATGGCGAGTACGAGGCTGACAGTGATGGCGGCGATGTTGCATGCGGTTGCAATTTTGCTGAAGCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

137

Amino Acids

15.22

Weight (kDa)

5.92

Isoelectric Point (pI)

58.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 372, 387
AcoI YGGCCR 1 cut(s) 257
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 1 cut(s) 277
AluBI AGCT 4 cut(s) 20, 56, 228, 273
AluI AGCT 4 cut(s) 20, 56, 228, 273
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 1 cut(s) 79
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 3 cut(s) 4, 184, 257
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 2 cut(s) 4, 293
AsuHPI GGTGA 2 cut(s) 187, 353
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 1 cut(s) 293
BalI TGGCCA 1 cut(s) 259
BanII GRGCYC 1 cut(s) 230
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 230
BccI CCATC 3 cut(s) 310, 338, 362
BcoDI GTCTC 1 cut(s) 79
BfaI CTAG 2 cut(s) 17, 231
BglI GCCNNNNNGGC 1 cut(s) 265
BglII AGATCT 1 cut(s) 238
BisI GCNGC 1 cut(s) 373
BlsI GCNGC 1 cut(s) 374
Bme18I GGWCC 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 4, 293
BmiI GGNNCC 1 cut(s) 300
BmrI ACTGGG 1 cut(s) 100
BmuI ACTGGG 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 79
BsaJI CCNNGG 2 cut(s) 95, 181
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 106
BseDI CCNNGG 2 cut(s) 95, 181
BseGI GGATG 2 cut(s) 12, 302
BseLI CCNNNNNNNGG 1 cut(s) 328
BseNI ACTGG 1 cut(s) 106
BshFI GGCC 3 cut(s) 6, 186, 259
BsiHKAI GWGCWC 1 cut(s) 230
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 2 cut(s) 173, 296
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 1 cut(s) 79
BsnI GGCC 3 cut(s) 6, 186, 259
Bso31I GGTCTC 1 cut(s) 79
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 230
Bsp143I GATC 2 cut(s) 238, 246
BspACI CCGC 2 cut(s) 372, 387
BspANI GGCC 3 cut(s) 6, 186, 259
BspLI GGNNCC 1 cut(s) 300
BspQI GCTCTTC 1 cut(s) 27
BspTNI GGTCTC 1 cut(s) 79
BsrI ACTGG 1 cut(s) 106
BssECI CCNNGG 2 cut(s) 95, 181
BssMI GATC 2 cut(s) 238, 246
BssT1I CCWWGG 1 cut(s) 95
Bst4CI ACNGT 3 cut(s) 205, 341, 365
Bst6I CTCTTC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 385, 409
BstF5I GGATG 2 cut(s) 12, 302
BstHHI GCGC 1 cut(s) 150
BstKTI GATC 2 cut(s) 241, 249
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 2 cut(s) 238, 246
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstNSI RCATGY 1 cut(s) 387
BstPAI GACNNNNGTC 1 cut(s) 291
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 238
BstXI CCANNNNNNTGG 1 cut(s) 309
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 3 cut(s) 6, 186, 259
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 2 cut(s) 12, 302
BtsIMutI CAGTG 1 cut(s) 370
Cac8I GCNNGC 2 cut(s) 385, 409
CfoI GCGC 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 4, 293
CpoI CGGWCCG 1 cut(s) 293
Csp6I GTAC 1 cut(s) 352
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CspI CGGWCCG 1 cut(s) 293
CviAII CATG 4 cut(s) 82, 119, 284, 384
CviQI GTAC 1 cut(s) 352
DpnI GATC 2 cut(s) 240, 248
DpnII GATC 2 cut(s) 238, 246
EaeI YGGCCR 1 cut(s) 257
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Ecl136II GAGCTC 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 230
Eco31I GGTCTC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 293
Eco53kI GAGCTC 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 230
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 4 cut(s) 85, 122, 287, 387
FaiI YATR 4 cut(s) 83, 120, 285, 385
FatI CATG 4 cut(s) 81, 118, 283, 383
Fnu4HI GCNGC 1 cut(s) 373
FokI GGATG 1 cut(s) 289
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 1 cut(s) 373
FspBI CTAG 2 cut(s) 17, 231
GlaI GCGC 1 cut(s) 149
GluI GCNGC 1 cut(s) 373
HaeIII GGCC 3 cut(s) 6, 186, 259
HapII CCGG 2 cut(s) 173, 296
HhaI GCGC 1 cut(s) 150
Hin1II CATG 4 cut(s) 85, 122, 287, 387
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HinfI GANTC 3 cut(s) 192, 221, 332
HpaII CCGG 2 cut(s) 173, 296
HphI GGTGA 2 cut(s) 187, 353
Hpy188I TCNGA 4 cut(s) 191, 238, 251, 337
Hpy188III TCNNGA 2 cut(s) 242, 244
Hpy99I CGWCG 1 cut(s) 341
HpyCH4III ACNGT 3 cut(s) 205, 341, 365
HpyCH4V TGCA 2 cut(s) 383, 393
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
Hsp92II CATG 4 cut(s) 85, 122, 287, 387
HspAI GCGC 1 cut(s) 148
Kzo9I GATC 2 cut(s) 238, 246
LguI GCTCTTC 1 cut(s) 27
LpnPI CCDG 5 cut(s) 119, 186, 309, 335, 393
MaeI CTAG 2 cut(s) 17, 231
MalI GATC 2 cut(s) 240, 248
MboI GATC 2 cut(s) 238, 246
MboII GAAGA 5 cut(s) 14, 17, 20, 74, 325
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 230
MlsI TGGCCA 1 cut(s) 259
MluCI AATT 1 cut(s) 394
MluNI TGGCCA 1 cut(s) 259
MmeI TCCRAC 2 cut(s) 69, 360
MnlI CCTC 8 cut(s) 17, 42, 59, 164, 191, 197, 258, 349
Mox20I TGGCCA 1 cut(s) 259
MscI TGGCCA 1 cut(s) 259
MslI CAYNNNNRTG 1 cut(s) 307
Msp20I TGGCCA 1 cut(s) 259
MspI CCGG 2 cut(s) 173, 296
MwoI GCNNNNNNNGC 1 cut(s) 265
NdeII GATC 2 cut(s) 238, 246
NlaIII CATG 4 cut(s) 85, 122, 287, 387
NlaIV GGNNCC 1 cut(s) 300
NmeAIII GCCGAG 1 cut(s) 162
NspI RCATGY 1 cut(s) 387
PaeI GCATGC 1 cut(s) 387
PaeR7I CTCGAG 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PfeI GAWTC 3 cut(s) 192, 221, 332
PkrI GCNGC 1 cut(s) 374
PshAI GACNNNNGTC 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 230
PspN4I GGNNCC 1 cut(s) 300
PspPI GGNCC 2 cut(s) 4, 293
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 307
Rsr2I CGGWCCG 1 cut(s) 293
RsrII CGGWCCG 1 cut(s) 293
SacI GAGCTC 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 27
SatI GCNGC 1 cut(s) 373
Sau3AI GATC 2 cut(s) 238, 246
Sau96I GGNCC 2 cut(s) 4, 293
SduI GDGCHC 1 cut(s) 230
SetI ASST 6 cut(s) 22, 51, 58, 156, 230, 275
Sfr274I CTCGAG 1 cut(s) 242
SinI GGWCC 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 307
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SphI GCATGC 1 cut(s) 387
Sse9I AATT 1 cut(s) 394
SsiI CCGC 2 cut(s) 372, 387
SspMI CTAG 2 cut(s) 17, 231
SstI GAGCTC 1 cut(s) 230
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 3 cut(s) 205, 341, 365
TaqI TCGA 2 cut(s) 224, 243
TaqII GACCGA 1 cut(s) 281
TasI AATT 1 cut(s) 394
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 3 cut(s) 192, 221, 332
TscAI CASTG 1 cut(s) 370
TspDTI ATGAA 2 cut(s) 121, 332
TspGWI ACGGA 1 cut(s) 344
TspRI CASTG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 293
XceI RCATGY 1 cut(s) 387
XhoI CTCGAG 1 cut(s) 242
XspI CTAG 2 cut(s) 17, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.