N-terminal C2 in EEIG1 and EHBP1 proteins
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| Species | Orthologous Gene IDs |
|---|---|
| rosa_laevigata | RLG00000000721 RLG00000001756 RLG00000022317 RLG00000033262 |
| rosa_multiflora | Rmu_sc0003259.1_g000012 |
| rosa_roxburghii | Rroxscaffold_3G00220710 Rroxscaffold_3G00245530 |
| rosa_rugosa | Rorug05G0151500 |
| rosa_samantha | Rh1BG024000 Rh1BG401400 Rh1DG431600 Rh2AG368500 Rh2BG554100 Rh2DG200800 Rh2DG559100 Rh3AG095200 Rh3CG099500 Rh3DG193000 Rh6AG458300 Rh7BG457500 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1880 |
| Acc16I | TGCGCA | 1 cut(s) | 1431 |
| AccB1I | GGYRCC | 1 cut(s) | 1746 |
| AccII | CGCG | 2 cut(s) | 474, 1789 |
| AciI | CCGC | 8 cut(s) | 253, 447, 472, 486, 863, 1046, 1668, 1776 |
| AclWI | GGATC | 4 cut(s) | 497, 548, 1636, 2033 |
| AcoI | YGGCCR | 5 cut(s) | 356, 469, 639, 1417, 2332 |
| AcsI | RAATTY | 3 cut(s) | 951, 1714, 1782 |
| AcuI | CTGAAG | 3 cut(s) | 798, 815, 1220 |
| AcyI | GRCGYC | 2 cut(s) | 105, 1747 |
| AdeI | CACNNNGTG | 1 cut(s) | 181 |
| AfaI | GTAC | 2 cut(s) | 86, 1320 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 100, 101, 990, 1274, 1617, 1651 |
| AgeI | ACCGGT | 1 cut(s) | 1331 |
| AgsI | TTSAA | 7 cut(s) | 7, 376, 1168, 1487, 1957, 2018, 2045 |
| AhlI | ACTAGT | 1 cut(s) | 1831 |
| AjnI | CCWGG | 2 cut(s) | 1258, 1884 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 312, 344 |
| Alw26I | GTCTC | 3 cut(s) | 178, 1137, 1354 |
| AlwI | GGATC | 4 cut(s) | 497, 548, 1636, 2033 |
| Ama87I | CYCGRG | 1 cut(s) | 2034 |
| AoxI | GGCC | 6 cut(s) | 356, 469, 639, 1417, 2123, 2332 |
| ApoI | RAATTY | 3 cut(s) | 951, 1714, 1782 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 371, 403, 1134, 1166 |
| AsiGI | ACCGGT | 1 cut(s) | 1331 |
| AspLEI | GCGC | 3 cut(s) | 249, 1432, 1749 |
| AspS9I | GGNCC | 3 cut(s) | 1150, 1897, 2123 |
| AsuC2I | CCSGG | 2 cut(s) | 1268, 1921 |
| AsuHPI | GGTGA | 6 cut(s) | 476, 627, 794, 1591, 1636, 1982 |
| AvaI | CYCGRG | 1 cut(s) | 2034 |
| AvaII | GGWCC | 2 cut(s) | 1150, 1897 |
| BalI | TGGCCA | 3 cut(s) | 358, 1419, 2334 |
| BanI | GGYRCC | 1 cut(s) | 1746 |
| BanII | GRGCYC | 1 cut(s) | 459 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1456, 1488 |
| BauI | CACGAG | 1 cut(s) | 341 |
| BbsI | GAAGAC | 4 cut(s) | 168, 803, 1242, 1917 |
| BbvCI | CCTCAGC | 1 cut(s) | 737 |
| BccI | CCATC | 3 cut(s) | 409, 437, 461 |
| BciT130I | CCWGG | 2 cut(s) | 1260, 1886 |
| BclI | TGATCA | 2 cut(s) | 345, 2161 |
| BcnI | CCSGG | 2 cut(s) | 1268, 1921 |
| BcoDI | GTCTC | 3 cut(s) | 178, 1137, 1354 |
| BcuI | ACTAGT | 1 cut(s) | 1831 |
| BfoI | RGCGCY | 1 cut(s) | 1750 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 364 |
| BglII | AGATCT | 1 cut(s) | 337 |
| BlpI | GCTNAGC | 1 cut(s) | 1704 |
| BmcAI | AGTACT | 1 cut(s) | 1320 |
| Bme1390I | CCNGG | 4 cut(s) | 1260, 1268, 1886, 1921 |
| Bme18I | GGWCC | 2 cut(s) | 1150, 1897 |
| BmeT110I | CYCGRG | 1 cut(s) | 2034 |
| BmgT120I | GGNCC | 3 cut(s) | 1150, 1897, 2123 |
| BmiI | GGNNCC | 5 cut(s) | 399, 1339, 1666, 1748, 1899 |
| BmrFI | CCNGG | 4 cut(s) | 1260, 1268, 1886, 1921 |
| BmrI | ACTGGG | 2 cut(s) | 199, 1984 |
| BmsI | GCATC | 3 cut(s) | 775, 1541, 2203 |
| BmuI | ACTGGG | 2 cut(s) | 199, 1984 |
| BpiI | GAAGAC | 4 cut(s) | 168, 803, 1242, 1917 |
| BpmI | CTGGAG | 4 cut(s) | 293, 514, 1722, 2027 |
| Bpu10I | CCTNAGC | 3 cut(s) | 237, 737, 1195 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1704 |
| BpuEI | CTTGAG | 1 cut(s) | 1667 |
| BpuMI | CCSGG | 2 cut(s) | 1268, 1921 |
| BsaHI | GRCGYC | 2 cut(s) | 105, 1747 |
| BsaI | GGTCTC | 1 cut(s) | 178 |
| BsaJI | CCNNGG | 3 cut(s) | 194, 1258, 1536 |
| BsaWI | WCCGGW | 1 cut(s) | 1331 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 597, 627, 1789, 1819 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 100, 101, 990, 1274, 1617, 1651 |
| Bse118I | RCCGGY | 3 cut(s) | 637, 641, 1331 |
| Bse1I | ACTGG | 6 cut(s) | 205, 1022, 1803, 1979, 2010, 2234 |
| Bse3DI | GCAATG | 2 cut(s) | 1420, 1839 |
| BseBI | CCWGG | 2 cut(s) | 1260, 1886 |
| BseDI | CCNNGG | 3 cut(s) | 194, 1258, 1536 |
| BseGI | GGATG | 8 cut(s) | 111, 401, 748, 1364, 1507, 1532, 1950, 2218 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 100, 101, 990, 1274, 1617, 1651 |
| BseMI | GCAATG | 2 cut(s) | 1420, 1839 |
| BseMII | CTCAG | 6 cut(s) | 228, 728, 842, 1106, 1695, 1907 |
| BseNI | ACTGG | 6 cut(s) | 205, 1022, 1803, 1979, 2010, 2234 |
| BseRI | GAGGAG | 5 cut(s) | 524, 973, 1403, 1618, 1677 |
| BseX3I | CGGCCG | 1 cut(s) | 639 |
| BsgI | GTGCAG | 2 cut(s) | 928, 1827 |
| Bsh1236I | CGCG | 2 cut(s) | 474, 1789 |
| Bsh1285I | CGRYCG | 1 cut(s) | 642 |
| BshFI | GGCC | 6 cut(s) | 358, 471, 641, 1419, 2125, 2334 |
| BshNI | GGYRCC | 1 cut(s) | 1746 |
| BshTI | ACCGGT | 1 cut(s) | 1331 |
| BsiEI | CGRYCG | 1 cut(s) | 642 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2034 |
| BsiSI | CCGG | 5 cut(s) | 638, 642, 1268, 1332, 1920 |
| BslFI | GGGAC | 1 cut(s) | 1225 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 100, 101, 990, 1274, 1617, 1651 |
| BsmAI | GTCTC | 3 cut(s) | 178, 1137, 1354 |
| BsmFI | GGGAC | 1 cut(s) | 1225 |
| BsmI | GAATGC | 1 cut(s) | 1189 |
| BsnI | GGCC | 6 cut(s) | 358, 471, 641, 1419, 2125, 2334 |
| Bso31I | GGTCTC | 1 cut(s) | 178 |
| BsoBI | CYCGRG | 1 cut(s) | 2034 |
| Bsp1286I | GDGCHC | 1 cut(s) | 459 |
| Bsp143I | GATC | 7 cut(s) | 337, 345, 489, 540, 1641, 2025, 2161 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1704 |
| BspACI | CCGC | 8 cut(s) | 253, 447, 472, 486, 863, 1046, 1668, 1776 |
| BspANI | GGCC | 6 cut(s) | 358, 471, 641, 1419, 2125, 2334 |
| BspCNI | CTCAG | 6 cut(s) | 229, 729, 841, 1105, 1696, 1906 |
| BspFNI | CGCG | 2 cut(s) | 474, 1789 |
| BspLI | GGNNCC | 5 cut(s) | 399, 1339, 1666, 1748, 1899 |
| BspPI | GGATC | 4 cut(s) | 497, 548, 1636, 2033 |
| BspQI | GCTCTTC | 1 cut(s) | 126 |
| BspT107I | GGYRCC | 1 cut(s) | 1746 |
| BspTNI | GGTCTC | 1 cut(s) | 178 |
| BsrDI | GCAATG | 2 cut(s) | 1420, 1839 |
| BsrFI | RCCGGY | 3 cut(s) | 637, 641, 1331 |
| BsrI | ACTGG | 6 cut(s) | 205, 1022, 1803, 1979, 2010, 2234 |
| BssAI | RCCGGY | 3 cut(s) | 637, 641, 1331 |
| BssECI | CCNNGG | 3 cut(s) | 194, 1258, 1536 |
| BssMI | GATC | 7 cut(s) | 337, 345, 489, 540, 1641, 2025, 2161 |
| BssNI | GRCGYC | 2 cut(s) | 105, 1747 |
| BssSI | CACGAG | 1 cut(s) | 341 |
| BssT1I | CCWWGG | 1 cut(s) | 194 |
| Bst2BI | CACGAG | 1 cut(s) | 341 |
| Bst2UI | CCWGG | 2 cut(s) | 1260, 1886 |
| Bst4CI | ACNGT | 3 cut(s) | 464, 1099, 1318 |
| Bst6I | CTCTTC | 4 cut(s) | 126, 687, 1357, 2192 |
| BstACI | GRCGYC | 2 cut(s) | 105, 1747 |
| BstC8I | GCNNGC | 5 cut(s) | 269, 484, 979, 1774, 2267 |
| BstDEI | CTNAG | 9 cut(s) | 237, 737, 805, 828, 1092, 1195, 1658, 1704, 1893 |
| BstDSI | CCRYGG | 1 cut(s) | 1536 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 988 |
| BstF5I | GGATG | 8 cut(s) | 111, 401, 748, 1364, 1507, 1532, 1950, 2218 |
| BstFNI | CGCG | 2 cut(s) | 474, 1789 |
| BstH2I | RGCGCY | 1 cut(s) | 1750 |
| BstHHI | GCGC | 3 cut(s) | 249, 1432, 1749 |
| BstKTI | GATC | 7 cut(s) | 340, 348, 492, 543, 1644, 2028, 2164 |
| BstMAI | GTCTC | 3 cut(s) | 178, 1137, 1354 |
| BstMBI | GATC | 7 cut(s) | 337, 345, 489, 540, 1641, 2025, 2161 |
| BstMCI | CGRYCG | 1 cut(s) | 642 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 364, 1043, 1052, 1410, 1808, 2096 |
| BstNI | CCWGG | 2 cut(s) | 1260, 1886 |
| BstNSI | RCATGY | 2 cut(s) | 1430, 1854 |
| BstSCI | CCNGG | 4 cut(s) | 1258, 1266, 1884, 1919 |
| BstUI | CGCG | 2 cut(s) | 474, 1789 |
| BstV2I | GAAGAC | 4 cut(s) | 168, 803, 1242, 1917 |
| BstX2I | RGATCY | 5 cut(s) | 337, 489, 540, 1641, 2025 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 408 |
| BstYI | RGATCY | 5 cut(s) | 337, 489, 540, 1641, 2025 |
| BstZI | CGGCCG | 1 cut(s) | 639 |
| BsuRI | GGCC | 6 cut(s) | 358, 471, 641, 1419, 2125, 2334 |
| BtgI | CCRYGG | 1 cut(s) | 1536 |
| BtgZI | GCGATG | 1 cut(s) | 1620 |
| BtsCI | GGATG | 8 cut(s) | 111, 401, 748, 1364, 1507, 1532, 1950, 2218 |
| BtsIMutI | CAGTG | 3 cut(s) | 1015, 1810, 2003 |
| Cac8I | GCNNGC | 5 cut(s) | 269, 484, 979, 1774, 2267 |
| CfoI | GCGC | 3 cut(s) | 249, 1432, 1749 |
| Cfr10I | RCCGGY | 3 cut(s) | 637, 641, 1331 |
| Cfr13I | GGNCC | 3 cut(s) | 1150, 1897, 2123 |
| CseI | GACGC | 3 cut(s) | 94, 485, 1957 |
| Csp6I | GTAC | 2 cut(s) | 85, 1319 |
| CspAI | ACCGGT | 1 cut(s) | 1331 |
| CviQI | GTAC | 2 cut(s) | 85, 1319 |
| DdeI | CTNAG | 9 cut(s) | 237, 737, 805, 828, 1092, 1195, 1658, 1704, 1893 |
| DinI | GGCGCC | 1 cut(s) | 1748 |
| DpnI | GATC | 7 cut(s) | 339, 347, 491, 542, 1643, 2027, 2163 |
| DpnII | GATC | 7 cut(s) | 337, 345, 489, 540, 1641, 2025, 2161 |
| DraIII | CACNNNGTG | 1 cut(s) | 181 |
| EaeI | YGGCCR | 5 cut(s) | 356, 469, 639, 1417, 2332 |
| EagI | CGGCCG | 1 cut(s) | 639 |
| Eam1104I | CTCTTC | 4 cut(s) | 126, 687, 1357, 2192 |
| EarI | CTCTTC | 4 cut(s) | 126, 687, 1357, 2192 |
| EclXI | CGGCCG | 1 cut(s) | 639 |
| Eco130I | CCWWGG | 1 cut(s) | 194 |
| Eco24I | GRGCYC | 1 cut(s) | 459 |
| Eco31I | GGTCTC | 1 cut(s) | 178 |
| Eco47I | GGWCC | 2 cut(s) | 1150, 1897 |
| Eco52I | CGGCCG | 1 cut(s) | 639 |
| Eco57I | CTGAAG | 3 cut(s) | 798, 815, 1220 |
| Eco88I | CYCGRG | 1 cut(s) | 2034 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 988 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1150, 1897 |
| EcoRII | CCWGG | 2 cut(s) | 1258, 1884 |
| EcoT14I | CCWWGG | 1 cut(s) | 194 |
| EcoT22I | ATGCAT | 1 cut(s) | 790 |
| EcoT38I | GRGCYC | 1 cut(s) | 459 |
| EgeI | GGCGCC | 1 cut(s) | 1748 |
| EheI | GGCGCC | 1 cut(s) | 1748 |
| ErhI | CCWWGG | 1 cut(s) | 194 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 788, 820, 1178, 1210, 2302, 2334 |
| FaqI | GGGAC | 1 cut(s) | 1225 |
| FauI | CCCGC | 2 cut(s) | 440, 479 |
| FauNDI | CATATG | 1 cut(s) | 2076 |
| FbaI | TGATCA | 2 cut(s) | 345, 2161 |
| FokI | GGATG | 8 cut(s) | 98, 388, 755, 1351, 1514, 1519, 1957, 2225 |
| FriOI | GRGCYC | 1 cut(s) | 459 |
| FspI | TGCGCA | 1 cut(s) | 1431 |
| GlaI | GCGC | 3 cut(s) | 248, 1431, 1748 |
| GsuI | CTGGAG | 4 cut(s) | 293, 514, 1722, 2027 |
| HaeII | RGCGCY | 1 cut(s) | 1750 |
| HaeIII | GGCC | 6 cut(s) | 358, 471, 641, 1419, 2125, 2334 |
| HapII | CCGG | 5 cut(s) | 638, 642, 1268, 1332, 1920 |
| HgaI | GACGC | 3 cut(s) | 94, 485, 1957 |
| HhaI | GCGC | 3 cut(s) | 249, 1432, 1749 |
| Hin1I | GRCGYC | 2 cut(s) | 105, 1747 |
| Hin6I | GCGC | 3 cut(s) | 247, 1430, 1747 |
| HinP1I | GCGC | 3 cut(s) | 247, 1430, 1747 |
| HincII | GTYRAC | 1 cut(s) | 1243 |
| HindII | GTYRAC | 1 cut(s) | 1243 |
| HindIII | AAGCTT | 2 cut(s) | 664, 997 |
| HinfI | GANTC | 6 cut(s) | 291, 320, 1391, 1627, 1679, 2018 |
| HpaII | CCGG | 5 cut(s) | 638, 642, 1268, 1332, 1920 |
| HphI | GGTGA | 6 cut(s) | 476, 627, 794, 1591, 1636, 1982 |
| Hpy166II | GTNNAC | 4 cut(s) | 679, 932, 1243, 1441 |
| Hpy8I | GTNNAC | 4 cut(s) | 679, 932, 1243, 1441 |
| Hpy99I | CGWCG | 1 cut(s) | 479 |
| HpyAV | CCTTC | 7 cut(s) | 85, 651, 773, 961, 1146, 1166, 1615 |
| HpyCH4III | ACNGT | 3 cut(s) | 464, 1099, 1318 |
| HpyCH4IV | ACGT | 1 cut(s) | 1991 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 364, 1043, 1052, 1410, 1808, 2096 |
| HpyF3I | CTNAG | 9 cut(s) | 237, 737, 805, 828, 1092, 1195, 1658, 1704, 1893 |
| HpySE526I | ACGT | 1 cut(s) | 1991 |
| Hsp92I | GRCGYC | 2 cut(s) | 105, 1747 |
| HspAI | GCGC | 3 cut(s) | 247, 1430, 1747 |
| KasI | GGCGCC | 1 cut(s) | 1746 |
| Ksp22I | TGATCA | 2 cut(s) | 345, 2161 |
| Kzo9I | GATC | 7 cut(s) | 337, 345, 489, 540, 1641, 2025, 2161 |
| LguI | GCTCTTC | 1 cut(s) | 126 |
| LmnI | GCTCC | 4 cut(s) | 986, 1654, 1664, 1741 |
| LweI | GCATC | 3 cut(s) | 775, 1541, 2203 |
| MaeII | ACGT | 1 cut(s) | 1991 |
| MaeIII | GTNAC | 7 cut(s) | 589, 720, 727, 1288, 1970, 2174, 2314 |
| MalI | GATC | 7 cut(s) | 339, 347, 491, 542, 1643, 2027, 2163 |
| MboI | GATC | 7 cut(s) | 337, 345, 489, 540, 1641, 2025, 2161 |
| MfeI | CAATTG | 1 cut(s) | 2100 |
| MflI | RGATCY | 5 cut(s) | 337, 489, 540, 1641, 2025 |
| MhlI | GDGCHC | 1 cut(s) | 459 |
| MlsI | TGGCCA | 3 cut(s) | 358, 1419, 2334 |
| MluNI | TGGCCA | 3 cut(s) | 358, 1419, 2334 |
| Mly113I | GGCGCC | 1 cut(s) | 1747 |
| MlyI | GAGTC | 2 cut(s) | 1400, 1688 |
| MmeI | TCCRAC | 3 cut(s) | 168, 556, 634 |
| Mox20I | TGGCCA | 3 cut(s) | 358, 1419, 2334 |
| Mph1103I | ATGCAT | 1 cut(s) | 790 |
| MscI | TGGCCA | 3 cut(s) | 358, 1419, 2334 |
| MseI | TTAA | 5 cut(s) | 39, 717, 812, 918, 962 |
| MslI | CAYNNNNRTG | 5 cut(s) | 406, 1167, 1403, 1425, 2256 |
| Msp20I | TGGCCA | 3 cut(s) | 358, 1419, 2334 |
| MspA1I | CMGCKG | 1 cut(s) | 1673 |
| MspI | CCGG | 5 cut(s) | 638, 642, 1268, 1332, 1920 |
| MspR9I | CCNGG | 4 cut(s) | 1260, 1268, 1886, 1921 |
| MunI | CAATTG | 1 cut(s) | 2100 |
| Mva1269I | GAATGC | 1 cut(s) | 1189 |
| MvaI | CCWGG | 2 cut(s) | 1260, 1886 |
| MvnI | CGCG | 2 cut(s) | 474, 1789 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 364, 1043, 1052, 1410, 1808, 2096 |
| NarI | GGCGCC | 1 cut(s) | 1747 |
| NciI | CCSGG | 2 cut(s) | 1268, 1921 |
| NdeI | CATATG | 1 cut(s) | 2076 |
| NdeII | GATC | 7 cut(s) | 337, 345, 489, 540, 1641, 2025, 2161 |
| NlaIV | GGNNCC | 5 cut(s) | 399, 1339, 1666, 1748, 1899 |
| NmeAIII | GCCGAG | 1 cut(s) | 1723 |
| NmuCI | GTSAC | 5 cut(s) | 589, 727, 1970, 2174, 2314 |
| NsbI | TGCGCA | 1 cut(s) | 1431 |
| NsiI | ATGCAT | 1 cut(s) | 790 |
| NspI | RCATGY | 2 cut(s) | 1430, 1854 |
| PciSI | GCTCTTC | 1 cut(s) | 126 |
| PctI | GAATGC | 1 cut(s) | 1189 |
| PfeI | GAWTC | 4 cut(s) | 291, 320, 1627, 2018 |
| PflFI | GACNNNGTC | 1 cut(s) | 2229 |
| PinAI | ACCGGT | 1 cut(s) | 1331 |
| PleI | GAGTC | 2 cut(s) | 1399, 1687 |
| PluTI | GGCGCC | 1 cut(s) | 1750 |
| PpsI | GAGTC | 2 cut(s) | 1399, 1687 |
| PpuMI | RGGWCCY | 2 cut(s) | 1150, 1897 |
| PsiI | TTATAA | 1 cut(s) | 1880 |
| Psp5II | RGGWCCY | 2 cut(s) | 1150, 1897 |
| Psp6I | CCWGG | 2 cut(s) | 1258, 1884 |
| PspGI | CCWGG | 2 cut(s) | 1258, 1884 |
| PspN4I | GGNNCC | 5 cut(s) | 399, 1339, 1666, 1748, 1899 |
| PspPI | GGNCC | 3 cut(s) | 1150, 1897, 2123 |
| PspPPI | RGGWCCY | 2 cut(s) | 1150, 1897 |
| PsuI | RGATCY | 5 cut(s) | 337, 489, 540, 1641, 2025 |
| PsyI | GACNNNGTC | 1 cut(s) | 2229 |
| PvuII | CAGCTG | 1 cut(s) | 1673 |
| RsaI | GTAC | 2 cut(s) | 86, 1320 |
| RsaNI | GTAC | 2 cut(s) | 85, 1319 |
| RseI | CAYNNNNRTG | 5 cut(s) | 406, 1167, 1403, 1425, 2256 |
| SapI | GCTCTTC | 1 cut(s) | 126 |
| SaqAI | TTAA | 5 cut(s) | 39, 717, 812, 918, 962 |
| Sau3AI | GATC | 7 cut(s) | 337, 345, 489, 540, 1641, 2025, 2161 |
| Sau96I | GGNCC | 3 cut(s) | 1150, 1897, 2123 |
| ScaI | AGTACT | 1 cut(s) | 1320 |
| SchI | GAGTC | 2 cut(s) | 1400, 1688 |
| ScrFI | CCNGG | 4 cut(s) | 1260, 1268, 1886, 1921 |
| SduI | GDGCHC | 1 cut(s) | 459 |
| SfaNI | GCATC | 3 cut(s) | 775, 1541, 2203 |
| SfoI | GGCGCC | 1 cut(s) | 1748 |
| SinI | GGWCC | 2 cut(s) | 1150, 1897 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 406, 1167, 1403, 1425, 2256 |
| SmlI | CTYRAG | 1 cut(s) | 1646 |
| SmoI | CTYRAG | 1 cut(s) | 1646 |
| SpeI | ACTAGT | 1 cut(s) | 1831 |
| SsiI | CCGC | 8 cut(s) | 253, 447, 472, 486, 863, 1046, 1668, 1776 |
| SspDI | GGCGCC | 1 cut(s) | 1746 |
| SspI | AATATT | 1 cut(s) | 2113 |
| StyD4I | CCNGG | 4 cut(s) | 1258, 1266, 1884, 1919 |
| StyI | CCWWGG | 1 cut(s) | 194 |
| TaaI | ACNGT | 3 cut(s) | 464, 1099, 1318 |
| TaiI | ACGT | 1 cut(s) | 1994 |
| TaqI | TCGA | 2 cut(s) | 323, 1373 |
| TatI | WGTACW | 1 cut(s) | 1318 |
| TauI | GCSGC | 3 cut(s) | 474, 866, 1670 |
| TfiI | GAWTC | 4 cut(s) | 291, 320, 1627, 2018 |
| Tru1I | TTAA | 5 cut(s) | 39, 717, 812, 918, 962 |
| Tru9I | TTAA | 5 cut(s) | 39, 717, 812, 918, 962 |
| TscAI | CASTG | 3 cut(s) | 1022, 1810, 2010 |
| TseFI | GTSAC | 5 cut(s) | 589, 727, 1970, 2174, 2314 |
| Tsp45I | GTSAC | 5 cut(s) | 589, 727, 1970, 2174, 2314 |
| TspGWI | ACGGA | 3 cut(s) | 169, 1525, 1974 |
| TspRI | CASTG | 3 cut(s) | 1022, 1810, 2010 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2229 |
| VpaK11BI | GGWCC | 2 cut(s) | 1150, 1897 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 988 |
| XapI | RAATTY | 3 cut(s) | 951, 1714, 1782 |
| XbaI | TCTAGA | 1 cut(s) | 2279 |
| XceI | RCATGY | 2 cut(s) | 1430, 1854 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 268 |
| ZrmI | AGTACT | 1 cut(s) | 1320 |
| Zsp2I | ATGCAT | 1 cut(s) | 790 |