Rh3DG193000

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0011622
Physical Location & Seq
Forward (+)
17269339 .. 17270602
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG193000.1

Sequence Viewer

Length: 408 bp
ATGAATATCTGGGATGGAATGGGCCTCATCATCCTAGCTCTTCTTCCTCCTCTCATTCAATCCAACCTCAAAGCTAAAGAAGACAAACAAAGCCACTCCGTGAGACCAAAACCAAGGAGAACCCAGTCTTTGCCTCATGTTTTGGCTTTCATTCCCGAGCAAGTGCGCCACCTCCACGCCACTCACTCGCCAGAGTTCCTCAGCCTCCGATTCACCGACACTGTTGGGCTTCTCAAAGAATCGAGCTCTGGTTCAGATCTCGTGATCAGAGTTTGGCCACAGAGGCAGAGCTTCAATAACCACGACTTCGGTTCGGTTCCATCCCAATGGAAGATGAACCAGACTACGGAATCCGACGGTGATGGCGGCGACGCTGCATGCGCTTGCGATTTTGTTGAAGCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

135

Amino Acids

14.99

Weight (kDa)

6.27

Isoelectric Point (pI)

68.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 366
AcoI YGGCCR 1 cut(s) 275
AdeI CACNNNGTG 1 cut(s) 100
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 3 cut(s) 59, 295, 398
AluBI AGCT 4 cut(s) 38, 74, 246, 291
AluI AGCT 4 cut(s) 38, 74, 246, 291
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 1 cut(s) 97
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 2 cut(s) 22, 275
ApeKI GCWGC 1 cut(s) 374
AspLEI GCGC 2 cut(s) 168, 383
AspS9I GGNCC 1 cut(s) 22
AsuHPI GGTGA 2 cut(s) 205, 371
AvaI CYCGRG 1 cut(s) 155
BalI TGGCCA 1 cut(s) 277
BanII GRGCYC 1 cut(s) 248
BauI CACGAG 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 248
BbvCI CCTCAGC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 361
BccI CCATC 3 cut(s) 8, 328, 356
BclI TGATCA 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 1 cut(s) 35
BglI GCCNNNNNGGC 1 cut(s) 283
BglII AGATCT 1 cut(s) 256
BisI GCNGC 2 cut(s) 367, 375
BlsI GCNGC 2 cut(s) 368, 376
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 22
BmiI GGNNCC 1 cut(s) 318
BmrI ACTGGG 1 cut(s) 118
BmuI ACTGGG 1 cut(s) 118
BpiI GAAGAC 1 cut(s) 87
Bpu10I CCTNAGC 1 cut(s) 200
BsaI GGTCTC 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 113
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 3 cut(s) 19, 30, 320
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMII CTCAG 1 cut(s) 214
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 361
BshFI GGCC 2 cut(s) 24, 277
BsiHKAI GWGCWC 1 cut(s) 248
BsiHKCI CYCGRG 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 97
BsnI GGCC 2 cut(s) 24, 277
Bso31I GGTCTC 1 cut(s) 97
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 248
Bsp143I GATC 2 cut(s) 256, 264
BspACI CCGC 1 cut(s) 366
BspANI GGCC 2 cut(s) 24, 277
BspCNI CTCAG 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 318
BspQI GCTCTTC 1 cut(s) 45
BspTNI GGTCTC 1 cut(s) 97
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 2 cut(s) 256, 264
BssSI CACGAG 1 cut(s) 260
BssT1I CCWWGG 1 cut(s) 113
Bst2BI CACGAG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 223, 359
Bst6I CTCTTC 1 cut(s) 45
BstC8I GCNNGC 3 cut(s) 379, 385, 403
BstDEI CTNAG 1 cut(s) 200
BstF5I GGATG 3 cut(s) 19, 30, 320
BstHHI GCGC 2 cut(s) 168, 383
BstKTI GATC 2 cut(s) 259, 267
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 2 cut(s) 256, 264
BstMWI GCNNNNNNNGC 2 cut(s) 283, 380
BstNSI RCATGY 1 cut(s) 381
BstV1I GCAGC 1 cut(s) 361
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 1 cut(s) 327
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 2 cut(s) 24, 277
BtsCI GGATG 3 cut(s) 19, 30, 320
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 3 cut(s) 379, 385, 403
CfoI GCGC 2 cut(s) 168, 383
Cfr13I GGNCC 1 cut(s) 22
CseI GACGC 1 cut(s) 380
CviAII CATG 2 cut(s) 137, 378
DdeI CTNAG 1 cut(s) 200
DpnI GATC 2 cut(s) 258, 266
DpnII GATC 2 cut(s) 256, 264
DraIII CACNNNGTG 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 248
Eco31I GGTCTC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 113
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 113
FaeI CATG 2 cut(s) 140, 381
FaiI YATR 2 cut(s) 138, 379
FatI CATG 2 cut(s) 136, 377
FbaI TGATCA 1 cut(s) 264
Fnu4HI GCNGC 2 cut(s) 367, 375
FokI GGATG 3 cut(s) 17, 26, 307
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 2 cut(s) 367, 375
FspBI CTAG 1 cut(s) 35
GlaI GCGC 2 cut(s) 167, 382
GluI GCNGC 2 cut(s) 367, 375
HaeIII GGCC 2 cut(s) 24, 277
HgaI GACGC 1 cut(s) 380
HhaI GCGC 2 cut(s) 168, 383
Hin1II CATG 2 cut(s) 140, 381
Hin6I GCGC 2 cut(s) 166, 381
HinP1I GCGC 2 cut(s) 166, 381
HinfI GANTC 3 cut(s) 210, 239, 350
HphI GGTGA 2 cut(s) 205, 371
Hpy188I TCNGA 4 cut(s) 209, 256, 269, 355
Hpy188III TCNNGA 2 cut(s) 155, 262
Hpy99I CGWCG 2 cut(s) 359, 374
HpyCH4III ACNGT 2 cut(s) 223, 359
HpyCH4V TGCA 1 cut(s) 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 380
HpyF3I CTNAG 1 cut(s) 200
Hsp92II CATG 2 cut(s) 140, 381
HspAI GCGC 2 cut(s) 166, 381
Ksp22I TGATCA 1 cut(s) 264
Kzo9I GATC 2 cut(s) 256, 264
LguI GCTCTTC 1 cut(s) 45
LpnPI CCDG 5 cut(s) 137, 204, 234, 353, 387
Lsp1109I GCAGC 1 cut(s) 361
MaeI CTAG 1 cut(s) 35
MalI GATC 2 cut(s) 258, 266
MboI GATC 2 cut(s) 256, 264
MboII GAAGA 4 cut(s) 32, 35, 92, 343
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 248
MlsI TGGCCA 1 cut(s) 277
MluNI TGGCCA 1 cut(s) 277
MmeI TCCRAC 2 cut(s) 87, 378
MnlI CCTC 9 cut(s) 35, 57, 60, 77, 144, 182, 209, 215, 276
Mox20I TGGCCA 1 cut(s) 277
MscI TGGCCA 1 cut(s) 277
MslI CAYNNNNRTG 1 cut(s) 325
Msp20I TGGCCA 1 cut(s) 277
MwoI GCNNNNNNNGC 2 cut(s) 283, 380
NdeII GATC 2 cut(s) 256, 264
NlaIII CATG 2 cut(s) 140, 381
NlaIV GGNNCC 1 cut(s) 318
NspI RCATGY 1 cut(s) 381
PaeI GCATGC 1 cut(s) 381
PciSI GCTCTTC 1 cut(s) 45
PfeI GAWTC 3 cut(s) 210, 239, 350
PkrI GCNGC 2 cut(s) 368, 376
Psp124BI GAGCTC 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 1 cut(s) 22
PsuI RGATCY 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 325
SacI GAGCTC 1 cut(s) 248
SapI GCTCTTC 1 cut(s) 45
SatI GCNGC 2 cut(s) 367, 375
Sau3AI GATC 2 cut(s) 256, 264
Sau96I GGNCC 1 cut(s) 22
SduI GDGCHC 1 cut(s) 248
SetI ASST 6 cut(s) 40, 69, 76, 174, 248, 293
SmiMI CAYNNNNRTG 1 cut(s) 325
SphI GCATGC 1 cut(s) 381
SsiI CCGC 1 cut(s) 366
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 248
StyI CCWWGG 1 cut(s) 113
TaaI ACNGT 2 cut(s) 223, 359
TaqI TCGA 1 cut(s) 242
TauI GCSGC 1 cut(s) 369
TfiI GAWTC 3 cut(s) 210, 239, 350
TscAI CASTG 1 cut(s) 226
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 3 cut(s) 17, 139, 350
TspGWI ACGGA 2 cut(s) 88, 362
TspRI CASTG 1 cut(s) 226
XceI RCATGY 1 cut(s) 381
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.