RLG00000033262

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011622
Physical Location & Seq
Reverse (-)
25069348 .. 25070165
818 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033262

Sequence Viewer

Length: 558 bp
ATGGAGATCGGAGCGGTGGTTGAGAACTCGATCTCATGTCACTGGCCTCACCTCCGATTCACCGACACTGTTGGGCTTCTCAAAGAATCGAGTTCTGGTTCAGATCTCATGATCAGAGTTTGGCCACAGAGGCAGAGCTTCAATGACCACGACTTCGGTCCGGTTCCATCCAAATGGAAGATGAACCAGACTACGGAATCCAACGGTCATGGAGGGTACGAGGCCGATTGTGATGGAGGCGACACTGCATGTGTTTTAGTAGTGGTGAAGGTAAGGATCTGGGGGATTAGAATTGAGGAGTTGAGAGGCAGTGGCTTGGAGAAGTGGGTCTGGGAATGGTTGTGGTGGAGGGTAAGGGTGGTCGGATTGGGTGTAGTGACAAATGGAGTAGGAGGTGGTGCAGCAACAAGTAGTGGTGGTGAGAAGGTGGTTTTGGAGATGGTGACTGTGAAGCTGCTCTCGGAGACTTTGTTCTTGTTCAAGTTTTGGTGGTGCAGCAACAGAAGGAATTCGGGTTTGAGTTCTTTTAGTAAGAGAGGAGGGAGAGAGGTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

186

Amino Acids

20.59

Weight (kDa)

7.71

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 14
AciI CCGC 1 cut(s) 14
AclWI GGATC 1 cut(s) 284
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 1 cut(s) 508
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 2 cut(s) 142, 481
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AloI GAACNNNNNNTCC 2 cut(s) 455, 487
AluBI AGCT 2 cut(s) 138, 454
AluI AGCT 2 cut(s) 138, 454
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 1 cut(s) 284
AoxI GGCC 3 cut(s) 44, 122, 222
ApeKI GCWGC 3 cut(s) 401, 454, 495
ApoI RAATTY 1 cut(s) 508
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
Asp700I GAANNNNTTC 1 cut(s) 508
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 5 cut(s) 41, 52, 277, 431, 454
AvaII GGWCC 1 cut(s) 158
BalI TGGCCA 1 cut(s) 124
BbvI GCAGC 3 cut(s) 413, 441, 507
BccI CCATC 3 cut(s) 175, 227, 433
BclI TGATCA 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 458
BglI GCCNNNNNGGC 1 cut(s) 130
BglII AGATCT 1 cut(s) 103
BisI GCNGC 3 cut(s) 402, 455, 496
BlsI GCNGC 3 cut(s) 403, 456, 497
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 165
BoxI GACNNNNGTC 1 cut(s) 156
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 3 cut(s) 33, 455, 485
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 47
BseGI GGATG 1 cut(s) 167
BseLI CCNNNNNNNGG 1 cut(s) 193
BseNI ACTGG 1 cut(s) 47
BseRI GAGGAG 2 cut(s) 311, 552
BseXI GCAGC 3 cut(s) 413, 441, 507
BsgI GTGCAG 2 cut(s) 420, 514
BshFI GGCC 3 cut(s) 46, 124, 224
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 458
BsnI GGCC 3 cut(s) 46, 124, 224
Bsp143I GATC 5 cut(s) 6, 30, 103, 111, 276
BspACI CCGC 1 cut(s) 14
BspANI GGCC 3 cut(s) 46, 124, 224
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 165
BspPI GGATC 1 cut(s) 284
BsrBI CCGCTC 1 cut(s) 14
BsrI ACTGG 1 cut(s) 47
BssMI GATC 5 cut(s) 6, 30, 103, 111, 276
Bst4CI ACNGT 3 cut(s) 70, 206, 448
BstF5I GGATG 1 cut(s) 167
BstKTI GATC 5 cut(s) 9, 33, 106, 114, 279
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 5 cut(s) 6, 30, 103, 111, 276
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNSI RCATGY 1 cut(s) 252
BstPAI GACNNNNGTC 1 cut(s) 156
BstV1I GCAGC 3 cut(s) 413, 441, 507
BstX2I RGATCY 2 cut(s) 103, 276
BstXI CCANNNNNNTGG 1 cut(s) 174
BstYI RGATCY 2 cut(s) 103, 276
BsuRI GGCC 3 cut(s) 46, 124, 224
BtsCI GGATG 1 cut(s) 167
BtsI GCAGTG 2 cut(s) 243, 316
BtsIMutI CAGTG 4 cut(s) 40, 66, 243, 316
CciI TCATGA 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 158
CpoI CGGWCCG 1 cut(s) 158
Csp6I GTAC 1 cut(s) 217
CspI CGGWCCG 1 cut(s) 158
CviAII CATG 4 cut(s) 36, 109, 209, 249
CviJI RGCY 7 cut(s) 46, 76, 124, 138, 224, 315, 454
CviKI_1 RGCY 7 cut(s) 46, 76, 124, 138, 224, 315, 454
CviQI GTAC 1 cut(s) 217
DpnI GATC 5 cut(s) 8, 32, 105, 113, 278
DpnII GATC 5 cut(s) 6, 30, 103, 111, 276
EaeI YGGCCR 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 508
FaeI CATG 4 cut(s) 39, 112, 212, 252
FaiI YATR 4 cut(s) 37, 110, 210, 250
FatI CATG 4 cut(s) 35, 108, 208, 248
FbaI TGATCA 1 cut(s) 111
Fnu4HI GCNGC 3 cut(s) 402, 455, 496
FokI GGATG 1 cut(s) 154
Fsp4HI GCNGC 3 cut(s) 402, 455, 496
GluI GCNGC 3 cut(s) 402, 455, 496
HaeIII GGCC 3 cut(s) 46, 124, 224
HapII CCGG 1 cut(s) 161
Hin1II CATG 4 cut(s) 39, 112, 212, 252
HinfI GANTC 3 cut(s) 57, 86, 197
HpaII CCGG 1 cut(s) 161
HphI GGTGA 5 cut(s) 41, 52, 277, 431, 454
Hpy188I TCNGA 6 cut(s) 11, 56, 103, 116, 365, 463
Hpy188III TCNNGA 1 cut(s) 109
HpyAV CCTTC 3 cut(s) 262, 418, 498
HpyCH4III ACNGT 3 cut(s) 70, 206, 448
HpyCH4V TGCA 3 cut(s) 248, 401, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
Hsp92II CATG 4 cut(s) 39, 112, 212, 252
Ksp22I TGATCA 1 cut(s) 111
Kzo9I GATC 5 cut(s) 6, 30, 103, 111, 276
LmnI GCTCC 1 cut(s) 11
LpnPI CCDG 6 cut(s) 28, 81, 174, 200, 265, 316
Lsp1109I GCAGC 3 cut(s) 413, 441, 507
MaeIII GTNAC 3 cut(s) 38, 376, 442
MalI GATC 5 cut(s) 8, 32, 105, 113, 278
MbiI CCGCTC 1 cut(s) 14
MboI GATC 5 cut(s) 6, 30, 103, 111, 276
MboII GAAGA 1 cut(s) 190
MflI RGATCY 2 cut(s) 103, 276
MlsI TGGCCA 1 cut(s) 124
MluCI AATT 2 cut(s) 291, 508
MluNI TGGCCA 1 cut(s) 124
MmeI TCCRAC 2 cut(s) 225, 343
Mox20I TGGCCA 1 cut(s) 124
MroXI GAANNNNTTC 1 cut(s) 508
MscI TGGCCA 1 cut(s) 124
MslI CAYNNNNRTG 1 cut(s) 172
Msp20I TGGCCA 1 cut(s) 124
MspI CCGG 1 cut(s) 161
MwoI GCNNNNNNNGC 1 cut(s) 130
NdeII GATC 5 cut(s) 6, 30, 103, 111, 276
NlaIII CATG 4 cut(s) 39, 112, 212, 252
NlaIV GGNNCC 1 cut(s) 165
NmuCI GTSAC 3 cut(s) 38, 376, 442
NspI RCATGY 1 cut(s) 252
PagI TCATGA 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 57, 86, 197
PkrI GCNGC 3 cut(s) 403, 456, 497
PshAI GACNNNNGTC 1 cut(s) 156
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 1 cut(s) 158
PsuI RGATCY 2 cut(s) 103, 276
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
RseI CAYNNNNRTG 1 cut(s) 172
Rsr2I CGGWCCG 1 cut(s) 158
RsrII CGGWCCG 1 cut(s) 158
SatI GCNGC 3 cut(s) 402, 455, 496
Sau3AI GATC 5 cut(s) 6, 30, 103, 111, 276
Sau96I GGNCC 1 cut(s) 158
SetI ASST 7 cut(s) 54, 140, 273, 397, 429, 456, 552
SinI GGWCC 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 172
Sse9I AATT 2 cut(s) 291, 508
SsiI CCGC 1 cut(s) 14
TaaI ACNGT 3 cut(s) 70, 206, 448
TaqI TCGA 2 cut(s) 29, 89
TaqII GACCGA 1 cut(s) 146
TasI AATT 2 cut(s) 291, 508
TfiI GAWTC 3 cut(s) 57, 86, 197
TscAI CASTG 4 cut(s) 47, 73, 250, 316
TseFI GTSAC 3 cut(s) 38, 376, 442
TseI GCWGC 3 cut(s) 401, 454, 495
Tsp45I GTSAC 3 cut(s) 38, 376, 442
TspDTI ATGAA 1 cut(s) 197
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 4 cut(s) 47, 73, 250, 316
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 1 cut(s) 508
XceI RCATGY 1 cut(s) 252
XmnI GAANNNNTTC 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.