Rroxscaffold_3G00220710

Subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011622
Physical Location & Seq
Reverse (-)
3074559 .. 3100038
25480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00220710.1

Sequence Viewer

Length: 870 bp
ATGAGAATTCTAAAAGCATGCTTGTATGCACATGTTAGGAGAAACATGATGATGATCGGGCTACTTAGGGCGGTGGTACTCGAGTCACGGCATTATTGTGAGTGGAGATTTGCTAAGTTGGAACCTGCTGCATTGGGGATTGAAGAACTGTTATTGCGGAACTATCATGAAGATGTTTCACATAGTTTCACTCACACCCGATCACGAAATAACCCTAGGTCTATGCGAGAGTACTTGACCTCTCATTCGTCACACAACCTCTCACTCGAAGACCTCTTTCTCACTCAACCGTTCTCACTCTTCAAGTACTACAGTTCAATCCGCACAACCCGAGCCTTCAGCTTCAACAACGTTGACCTTCACCTTCGATCTGCACAATGTCGACCTTCAAACCTCCTTCGATCTGCAGTTCTACACAACGCTGCCGTCGCCGTTTTGTCTCGCCACCGATATGGACTTGAAGCCCCTATTATCAATGAGAAAACACAGCAAATTTCTCCCAAATTCGGTTTCTACAACACGTTATCGGCACGAGCCCTAACCCCGAAACCCTATATTCCTAAAGAAGATAAAGAAAGCCACTCCGTGAGACCAAAACCGAAGAGAACCCGGTCTTTGCCTCATGTTTTGGCTCTCATTCCCGAGCAAGTGCACCGCATCCATACCACTCGCTCGTCGGAGTTCCTCGGCCTCCGGTTCACCGACACTACGGGCTTCTCAAAGAATTGGACTCGGTTCATATCTCATGATCGAGTTTGGCCACATAGGCGAGCTTCGGCGACCACGACCTCGAATATAGCTAGTTTTACGAATGATGCTCTCAATGATCAAAACCGACAACTTCATTGCCAAAACGCCCTACAGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

289

Amino Acids

33.58

Weight (kDa)

10.32

Isoelectric Point (pI)

52.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 133
AccI GTMKAC 1 cut(s) 382
AciI CCGC 4 cut(s) 71, 157, 322, 655
AclI AACGTT 1 cut(s) 351
AcoI YGGCCR 1 cut(s) 758
AcsI RAATTY 3 cut(s) 6, 492, 503
AcuI CTGAAG 1 cut(s) 322
AdeI CACNNNGTG 1 cut(s) 586
AfaI GTAC 3 cut(s) 78, 233, 308
AfiI CCNNNNNNNGG 1 cut(s) 506
AflIII ACRYGT 2 cut(s) 31, 519
AgsI TTSAA 6 cut(s) 143, 304, 318, 346, 390, 461
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AluBI AGCT 3 cut(s) 342, 773, 800
AluI AGCT 3 cut(s) 342, 773, 800
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 2 cut(s) 444, 583
Alw44I GTGCAC 1 cut(s) 650
Ama87I CYCGRG 3 cut(s) 80, 330, 641
AoxI GGCC 2 cut(s) 688, 758
ApaLI GTGCAC 1 cut(s) 650
ApeKI GCWGC 2 cut(s) 128, 422
ApoI RAATTY 3 cut(s) 6, 492, 503
ArsI GACNNNNNNTTYG 2 cut(s) 229, 261
AspA2I CCTAGG 1 cut(s) 215
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 2 cut(s) 353, 691
AvaI CYCGRG 3 cut(s) 80, 330, 641
AvrII CCTAGG 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 654
BaeI ACNNNNGTAYC 2 cut(s) 68, 101
BalI TGGCCA 1 cut(s) 760
BanII GRGCYC 1 cut(s) 538
BauI CACGAG 1 cut(s) 531
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 2 cut(s) 115, 409
BceAI ACGGC 3 cut(s) 104, 410, 416
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 2 cut(s) 444, 583
BfaI CTAG 2 cut(s) 216, 801
BfmI CTRYAG 3 cut(s) 310, 405, 860
BfuAI ACCTGC 1 cut(s) 133
BglI GCCNNNNNGGC 1 cut(s) 766
BisI GCNGC 2 cut(s) 129, 423
BlnI CCTAGG 1 cut(s) 215
BlsI GCNGC 2 cut(s) 130, 424
BmcAI AGTACT 2 cut(s) 233, 308
Bme1390I CCNGG 1 cut(s) 610
BmeT110I CYCGRG 3 cut(s) 80, 330, 641
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 610
BmsI GCATC 2 cut(s) 666, 805
BpiI GAAGAC 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 610
BsaBI GATNNNNATC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 583
BsaJI CCNNGG 2 cut(s) 215, 685
BsaWI WCCGGW 1 cut(s) 693
Bsc4I CCNNNNNNNGG 1 cut(s) 506
Bse3DI GCAATG 1 cut(s) 844
Bse8I GATNNNNATC 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 215, 685
BseGI GGATG 1 cut(s) 657
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 506
BseMI GCAATG 1 cut(s) 844
BseSI GKGCMC 1 cut(s) 654
BseXI GCAGC 2 cut(s) 115, 409
BsgI GTGCAG 1 cut(s) 357
BshFI GGCC 2 cut(s) 690, 760
BsiHKAI GWGCWC 1 cut(s) 654
BsiHKCI CYCGRG 3 cut(s) 80, 330, 641
BsiSI CCGG 2 cut(s) 610, 694
BslI CCNNNNNNNGG 1 cut(s) 506
BsmAI GTCTC 2 cut(s) 444, 583
BsnI GGCC 2 cut(s) 690, 760
Bso31I GGTCTC 1 cut(s) 583
BsoBI CYCGRG 3 cut(s) 80, 330, 641
Bsp1286I GDGCHC 2 cut(s) 538, 654
Bsp143I GATC 6 cut(s) 54, 200, 368, 401, 748, 826
BspACI CCGC 4 cut(s) 71, 157, 322, 655
BspANI GGCC 2 cut(s) 690, 760
BspHI TCATGA 2 cut(s) 166, 745
BspLI GGNNCC 1 cut(s) 123
BspMAI CTGCAG 1 cut(s) 409
BspMI ACCTGC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 583
BsrDI GCAATG 1 cut(s) 844
BssECI CCNNGG 2 cut(s) 215, 685
BssMI GATC 6 cut(s) 54, 200, 368, 401, 748, 826
BssSI CACGAG 1 cut(s) 531
BssT1I CCWWGG 1 cut(s) 215
Bst2BI CACGAG 1 cut(s) 531
Bst4CI ACNGT 3 cut(s) 150, 291, 314
Bst6I CTCTTC 2 cut(s) 305, 596
BstC8I GCNNGC 2 cut(s) 19, 771
BstDEI CTNAG 2 cut(s) 65, 114
BstF5I GGATG 1 cut(s) 657
BstKTI GATC 6 cut(s) 57, 203, 371, 404, 751, 829
BstMAI GTCTC 2 cut(s) 444, 583
BstMBI GATC 6 cut(s) 54, 200, 368, 401, 748, 826
BstMWI GCNNNNNNNGC 2 cut(s) 428, 766
BstNSI RCATGY 2 cut(s) 21, 35
BstSCI CCNGG 1 cut(s) 608
BstSFI CTRYAG 3 cut(s) 310, 405, 860
BstSLI GKGCMC 1 cut(s) 654
BstV1I GCAGC 2 cut(s) 115, 409
BstV2I GAAGAC 1 cut(s) 276
BstXI CCANNNNNNTGG 1 cut(s) 452
BsuRI GGCC 2 cut(s) 690, 760
BtsCI GGATG 1 cut(s) 657
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 19, 771
CciI TCATGA 2 cut(s) 166, 745
Csp6I GTAC 3 cut(s) 77, 232, 307
CviAII CATG 6 cut(s) 18, 32, 46, 167, 623, 746
CviQI GTAC 3 cut(s) 77, 232, 307
DdeI CTNAG 2 cut(s) 65, 114
DpnI GATC 6 cut(s) 56, 202, 370, 403, 750, 828
DpnII GATC 6 cut(s) 54, 200, 368, 401, 748, 826
DraIII CACNNNGTG 1 cut(s) 586
EaeI YGGCCR 1 cut(s) 758
Eam1104I CTCTTC 2 cut(s) 305, 596
EarI CTCTTC 2 cut(s) 305, 596
Eco130I CCWWGG 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 538
Eco31I GGTCTC 1 cut(s) 583
Eco57I CTGAAG 1 cut(s) 322
Eco88I CYCGRG 3 cut(s) 80, 330, 641
EcoRI GAATTC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 215
EcoT38I GRGCYC 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 215
FaeI CATG 6 cut(s) 21, 35, 49, 170, 626, 749
FatI CATG 6 cut(s) 17, 31, 45, 166, 622, 745
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 129, 423
FokI GGATG 1 cut(s) 644
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 2 cut(s) 129, 423
FspBI CTAG 2 cut(s) 216, 801
GluI GCNGC 2 cut(s) 129, 423
HaeIII GGCC 2 cut(s) 690, 760
HapII CCGG 2 cut(s) 610, 694
Hin1II CATG 6 cut(s) 21, 35, 49, 170, 626, 749
HincII GTYRAC 2 cut(s) 355, 383
HindII GTYRAC 2 cut(s) 355, 383
HinfI GANTC 2 cut(s) 83, 730
HpaII CCGG 2 cut(s) 610, 694
HphI GGTGA 2 cut(s) 353, 691
Hpy166II GTNNAC 4 cut(s) 355, 383, 652, 699
Hpy188I TCNGA 1 cut(s) 679
Hpy188III TCNNGA 4 cut(s) 167, 204, 641, 746
Hpy8I GTNNAC 4 cut(s) 355, 383, 652, 699
Hpy99I CGWCG 2 cut(s) 431, 679
HpyAV CCTTC 5 cut(s) 346, 368, 374, 396, 407
HpyCH4III ACNGT 3 cut(s) 150, 291, 314
HpyCH4IV ACGT 2 cut(s) 351, 521
HpyCH4V TGCA 5 cut(s) 29, 131, 374, 407, 652
HpyF10VI GCNNNNNNNGC 2 cut(s) 428, 766
HpyF3I CTNAG 2 cut(s) 65, 114
HpySE526I ACGT 2 cut(s) 351, 521
Hsp92II CATG 6 cut(s) 21, 35, 49, 170, 626, 749
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 6 cut(s) 54, 200, 368, 401, 748, 826
LpnPI CCDG 3 cut(s) 138, 623, 707
Lsp1109I GCAGC 2 cut(s) 115, 409
LweI GCATC 2 cut(s) 666, 805
MaeI CTAG 2 cut(s) 216, 801
MaeII ACGT 2 cut(s) 351, 521
MaeIII GTNAC 2 cut(s) 84, 249
MalI GATC 6 cut(s) 56, 202, 370, 403, 750, 828
MboI GATC 6 cut(s) 54, 200, 368, 401, 748, 826
MboII GAAGA 6 cut(s) 155, 182, 281, 292, 578, 613
MhlI GDGCHC 2 cut(s) 538, 654
MlsI TGGCCA 1 cut(s) 760
MluCI AATT 4 cut(s) 6, 492, 503, 724
MluNI TGGCCA 1 cut(s) 760
MlyI GAGTC 2 cut(s) 92, 724
MmeI TCCRAC 2 cut(s) 99, 657
MnlI CCTC 8 cut(s) 250, 269, 284, 404, 630, 695, 701, 799
Mox20I TGGCCA 1 cut(s) 760
MscI TGGCCA 1 cut(s) 760
MslI CAYNNNNRTG 4 cut(s) 50, 96, 171, 450
Msp20I TGGCCA 1 cut(s) 760
MspI CCGG 2 cut(s) 610, 694
MspR9I CCNGG 1 cut(s) 610
MwoI GCNNNNNNNGC 2 cut(s) 428, 766
NciI CCSGG 1 cut(s) 610
NdeII GATC 6 cut(s) 54, 200, 368, 401, 748, 826
NlaIII CATG 6 cut(s) 21, 35, 49, 170, 626, 749
NlaIV GGNNCC 1 cut(s) 123
NmeAIII GCCGAG 1 cut(s) 666
NmuCI GTSAC 2 cut(s) 84, 249
NspI RCATGY 2 cut(s) 21, 35
PaeI GCATGC 1 cut(s) 21
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 2 cut(s) 166, 745
PciI ACATGT 1 cut(s) 31
PcsI WCGNNNNNNNCGW 1 cut(s) 782
PkrI GCNGC 2 cut(s) 130, 424
PleI GAGTC 2 cut(s) 91, 724
PpsI GAGTC 2 cut(s) 91, 724
PscI ACATGT 1 cut(s) 31
Psp1406I AACGTT 1 cut(s) 351
PspN4I GGNNCC 1 cut(s) 123
PspXI VCTCGAGB 1 cut(s) 80
PstI CTGCAG 1 cut(s) 409
RsaI GTAC 3 cut(s) 78, 233, 308
RsaNI GTAC 3 cut(s) 77, 232, 307
RseI CAYNNNNRTG 4 cut(s) 50, 96, 171, 450
SalI GTCGAC 1 cut(s) 381
SatI GCNGC 2 cut(s) 129, 423
Sau3AI GATC 6 cut(s) 54, 200, 368, 401, 748, 826
ScaI AGTACT 2 cut(s) 233, 308
SchI GAGTC 2 cut(s) 92, 724
ScrFI CCNGG 1 cut(s) 610
SduI GDGCHC 2 cut(s) 538, 654
SfaNI GCATC 2 cut(s) 666, 805
SfcI CTRYAG 3 cut(s) 310, 405, 860
Sfr274I CTCGAG 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 4 cut(s) 50, 96, 171, 450
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SphI GCATGC 1 cut(s) 21
Sse9I AATT 4 cut(s) 6, 492, 503, 724
SsiI CCGC 4 cut(s) 71, 157, 322, 655
SspMI CTAG 2 cut(s) 216, 801
StyD4I CCNGG 1 cut(s) 608
StyI CCWWGG 1 cut(s) 215
TaaI ACNGT 3 cut(s) 150, 291, 314
TaiI ACGT 2 cut(s) 354, 524
TaqI TCGA 7 cut(s) 81, 267, 367, 382, 400, 751, 791
TasI AATT 4 cut(s) 6, 492, 503, 724
TatI WGTACW 2 cut(s) 231, 306
TseFI GTSAC 2 cut(s) 84, 249
TseI GCWGC 2 cut(s) 128, 422
Tsp45I GTSAC 2 cut(s) 84, 249
TspDTI ATGAA 3 cut(s) 183, 727, 833
TspGWI ACGGA 1 cut(s) 574
VneI GTGCAC 1 cut(s) 650
XapI RAATTY 3 cut(s) 6, 492, 503
XceI RCATGY 2 cut(s) 21, 35
XhoI CTCGAG 1 cut(s) 80
XmaJI CCTAGG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 382
XspI CTAG 2 cut(s) 216, 801
ZrmI AGTACT 2 cut(s) 233, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.