RLG00000007424

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003469
Physical Location & Seq
Reverse (-)
16634069 .. 16637366
3298 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007424

Sequence Viewer

Length: 606 bp
ATGTTGTCAGAACTATCACAGCAAGGTAGATTCATTGATTTGGAGAACTTTGGTTTAACAATAGTCAATTGCTTTGGATTGGTTGACGATGAAGGCTTGAATAATGGAATATTTTCATTGCTAACGAGATTGGCAGCTCAGCTGCACCTTTTGTTTTGTCTCCCACTAACAGCAGCTCCACCTTTTGTCCTTCTCGAGAAGTGGCCATTCGAGGATCACCCGCCCCTTGGGAAACCAACTGACAAAGGGCAAAACCGCAAGATATTGCTAAGGTATCAGAAGCCAGAAGGTTCTGATGAGCATGCAAGTGGCAAGGGCTCAGCGCCACCACAGCCTGCTTCTGAGAACGGCGAAGGTGAGCGAAAGAAGAACGGCATAGGTGAGCAAAAGAAAAAGGGCAAAGGTGAGCAAAAGACGAATGGCAATAAGAAAAAGAAAGGCAAAGGGAAAGCTGATGTTTTCAATATTCAGGGCAACAAATTCGATGGTGGCAGCGGCAAAGCTACCAAGTCCGGAGTTTTTTATTTTAATAATACGTATAGTGGCCACAAAGTATATGAGTCAAGTGATGAAAAAGGTGACTCCTGTGATGAAGAGGCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

202

Amino Acids

21.95

Weight (kDa)

7.67

Isoelectric Point (pI)

27.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 512
AciI CCGC 3 cut(s) 221, 256, 495
AclWI GGATC 1 cut(s) 222
AcoI YGGCCR 2 cut(s) 203, 544
AcsI RAATTY 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 227
AgsI TTSAA 2 cut(s) 100, 463
AluBI AGCT 5 cut(s) 137, 142, 176, 452, 503
AluI AGCT 5 cut(s) 137, 142, 176, 452, 503
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 194
Aor13HI TCCGGA 1 cut(s) 512
AoxI GGCC 2 cut(s) 203, 544
ApeKI GCWGC 4 cut(s) 134, 142, 173, 492
ApoI RAATTY 1 cut(s) 479
Asp700I GAANNNNTTC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 325
AsuHPI GGTGA 5 cut(s) 209, 368, 392, 416, 590
AvaI CYCGRG 1 cut(s) 194
BalI TGGCCA 2 cut(s) 205, 546
BanII GRGCYC 1 cut(s) 320
BbvI GCAGC 4 cut(s) 129, 146, 185, 504
BccI CCATC 1 cut(s) 479
BceAI ACGGC 2 cut(s) 364, 388
BcgI CGANNNNNNTGC 2 cut(s) 463, 497
BcoDI GTCTC 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 326
BisI GCNGC 5 cut(s) 135, 143, 174, 493, 496
BlpI GCTNAGC 2 cut(s) 138, 319
BlsI GCNGC 5 cut(s) 136, 144, 175, 494, 497
BmeT110I CYCGRG 1 cut(s) 194
Bpu10I CCTNAGC 1 cut(s) 269
Bpu1102I GCTNAGC 2 cut(s) 138, 319
BsaAI YACGTR 1 cut(s) 537
BsaJI CCNNGG 1 cut(s) 226
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 160, 190
Bsc4I CCNNNNNNNGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 512
BseDI CCNNGG 1 cut(s) 226
BseLI CCNNNNNNNGG 1 cut(s) 227
BseMI GCAATG 1 cut(s) 116
BseMII CTCAG 3 cut(s) 152, 333, 333
BseXI GCAGC 4 cut(s) 129, 146, 185, 504
BsgI GTGCAG 1 cut(s) 128
BshFI GGCC 2 cut(s) 205, 546
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 1 cut(s) 513
BslI CCNNNNNNNGG 1 cut(s) 227
BsmAI GTCTC 1 cut(s) 164
BsnI GGCC 2 cut(s) 205, 546
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 320
Bsp13I TCCGGA 1 cut(s) 512
Bsp143I GATC 1 cut(s) 214
Bsp1720I GCTNAGC 2 cut(s) 138, 319
BspACI CCGC 3 cut(s) 221, 256, 495
BspANI GGCC 2 cut(s) 205, 546
BspCNI CTCAG 3 cut(s) 151, 332, 334
BspEI TCCGGA 1 cut(s) 512
BspPI GGATC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 116
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 226
Bst6I CTCTTC 1 cut(s) 588
BstBAI YACGTR 1 cut(s) 537
BstC8I GCNNGC 2 cut(s) 303, 336
BstDEI CTNAG 4 cut(s) 138, 269, 319, 342
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 1 cut(s) 217
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 331
BstNSI RCATGY 1 cut(s) 305
BstSNI TACGTA 1 cut(s) 537
BstV1I GCAGC 4 cut(s) 129, 146, 185, 504
BsuRI GGCC 2 cut(s) 205, 546
Cac8I GCNNGC 2 cut(s) 303, 336
CfoI GCGC 1 cut(s) 325
CviAII CATG 1 cut(s) 302
DdeI CTNAG 4 cut(s) 138, 269, 319, 342
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 203, 544
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco105I TACGTA 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 1 cut(s) 305
FaiI YATR 5 cut(s) 303, 377, 540, 556, 558
FatI CATG 1 cut(s) 301
FauI CCCGC 1 cut(s) 228
Fnu4HI GCNGC 5 cut(s) 135, 143, 174, 493, 496
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 5 cut(s) 135, 143, 174, 493, 496
GlaI GCGC 1 cut(s) 324
GluI GCNGC 5 cut(s) 135, 143, 174, 493, 496
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 2 cut(s) 205, 546
HapII CCGG 1 cut(s) 513
HhaI GCGC 1 cut(s) 325
Hin1II CATG 1 cut(s) 305
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 3 cut(s) 30, 560, 581
HpaII CCGG 1 cut(s) 513
HphI GGTGA 5 cut(s) 209, 368, 392, 416, 590
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 4 cut(s) 10, 279, 295, 343
Hpy188III TCNNGA 3 cut(s) 194, 196, 513
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 86, 200, 281, 347
HpyCH4IV ACGT 1 cut(s) 536
HpyCH4V TGCA 2 cut(s) 145, 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 331
HpyF3I CTNAG 4 cut(s) 138, 269, 319, 342
HpySE526I ACGT 1 cut(s) 536
Hsp92II CATG 1 cut(s) 305
HspAI GCGC 1 cut(s) 323
Kpn2I TCCGGA 1 cut(s) 512
Kzo9I GATC 1 cut(s) 214
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 5 cut(s) 297, 348, 455, 526, 598
Lsp1109I GCAGC 4 cut(s) 129, 146, 185, 504
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 1 cut(s) 578
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 2 cut(s) 379, 605
MfeI CAATTG 1 cut(s) 67
MhlI GDGCHC 1 cut(s) 320
MlsI TGGCCA 2 cut(s) 205, 546
MluCI AATT 2 cut(s) 67, 479
MluNI TGGCCA 2 cut(s) 205, 546
MlyI GAGTC 2 cut(s) 569, 575
MnlI CCTC 2 cut(s) 205, 589
Mox20I TGGCCA 2 cut(s) 205, 546
MroI TCCGGA 1 cut(s) 512
MroXI GAANNNNTTC 1 cut(s) 112
MscI TGGCCA 2 cut(s) 205, 546
MseI TTAA 2 cut(s) 56, 528
MslI CAYNNNNRTG 1 cut(s) 306
Msp20I TGGCCA 2 cut(s) 205, 546
MspA1I CMGCKG 2 cut(s) 142, 495
MspI CCGG 1 cut(s) 513
MunI CAATTG 1 cut(s) 67
MwoI GCNNNNNNNGC 1 cut(s) 331
NdeII GATC 1 cut(s) 214
NlaIII CATG 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 578
NspI RCATGY 1 cut(s) 305
PaeI GCATGC 1 cut(s) 305
PaeR7I CTCGAG 1 cut(s) 194
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 30
PkrI GCNGC 5 cut(s) 136, 144, 175, 494, 497
PleI GAGTC 2 cut(s) 568, 575
PpsI GAGTC 2 cut(s) 568, 575
Ppu21I YACGTR 1 cut(s) 537
PvuII CAGCTG 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 306
SaqAI TTAA 2 cut(s) 56, 528
SatI GCNGC 5 cut(s) 135, 143, 174, 493, 496
Sau3AI GATC 1 cut(s) 214
SchI GAGTC 2 cut(s) 569, 575
SduI GDGCHC 1 cut(s) 320
Sfr274I CTCGAG 1 cut(s) 194
SlaI CTCGAG 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SnaBI TACGTA 1 cut(s) 537
SphI GCATGC 1 cut(s) 305
Sse9I AATT 2 cut(s) 67, 479
SsiI CCGC 3 cut(s) 221, 256, 495
SspI AATATT 2 cut(s) 111, 466
StyI CCWWGG 1 cut(s) 226
TaiI ACGT 1 cut(s) 539
TaqI TCGA 3 cut(s) 195, 210, 483
TasI AATT 2 cut(s) 67, 479
TauI GCSGC 1 cut(s) 498
TfiI GAWTC 1 cut(s) 30
Tru1I TTAA 2 cut(s) 56, 528
Tru9I TTAA 2 cut(s) 56, 528
TseFI GTSAC 1 cut(s) 578
TseI GCWGC 4 cut(s) 134, 142, 173, 492
Tsp45I GTSAC 1 cut(s) 578
TspDTI ATGAA 5 cut(s) 22, 105, 105, 585, 606
XapI RAATTY 1 cut(s) 479
XceI RCATGY 1 cut(s) 305
XhoI CTCGAG 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.