Rh4DG256700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003469
Physical Location & Seq
Reverse (-)
47293557 .. 47294138
582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG256700.1

Sequence Viewer

Length: 399 bp
ATGGATCAAAGGGGCAAGAGCTCAAAGCCACCACAGCCTGCTTCTAAGAACGGCGAAGGTGAGCAAAAGAAGAACGGCATAGGTGAGCAAAAGAAAAACGGCAAAGGTGAGCAAAAGACGAATGGCAACAAGAAACAGGAAGGCAAAGGGAACGCTGATGCTAACCCGGTTCAGGACAACAAAATCAATAATAATAATACAGCTACAAGTGTTGGAGTTTTTGGATTCGGTAACAAGGAGTATTATGGCCCCAAAGAGTCAAGTGATGAAGAAGAAGGTGACTCCGGTGATGCATACACGGTTCAGAGAAACGAAATCAATAATCAAGGTATAGCTGAACGTGTCGGAGTTTTTGACTTTGCTAACAAGAAGCATTTGGGCGCCAAATCTGAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.19

Weight (kDa)

7.84

Isoelectric Point (pI)

27.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 380
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 381
AfiI CCNNNNNNNGG 1 cut(s) 172
AflIII ACRYGT 1 cut(s) 340
AluBI AGCT 3 cut(s) 21, 203, 335
AluI AGCT 3 cut(s) 21, 203, 335
Alw21I GWGCWC 1 cut(s) 23
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 247
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 248
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 5 cut(s) 71, 95, 119, 290, 299
BanI GGYRCC 1 cut(s) 380
BanII GRGCYC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 23
BceAI ACGGC 3 cut(s) 67, 91, 115
BcnI CCSGG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 384
Bme1390I CCNGG 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 2 cut(s) 250, 382
BmrFI CCNGG 1 cut(s) 167
BmsI GCATC 2 cut(s) 148, 280
BpuMI CCSGG 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 381
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 172
BshFI GGCC 1 cut(s) 249
BshNI GGYRCC 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 2 cut(s) 167, 285
BslI CCNNNNNNNGG 1 cut(s) 172
BsnI GGCC 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 1 cut(s) 4
BspANI GGCC 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 250, 382
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 380
BssMI GATC 1 cut(s) 4
BssNI GRCGYC 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 301
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 1 cut(s) 45
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 1 cut(s) 7
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 165
BsuRI GGCC 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 39
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 248
CviJI RGCY 6 cut(s) 21, 28, 37, 203, 249, 335
CviKI_1 RGCY 6 cut(s) 21, 28, 37, 203, 249, 335
DdeI CTNAG 1 cut(s) 45
DinI GGCGCC 1 cut(s) 382
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 23
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 23
EgeI GGCGCC 1 cut(s) 382
EheI GGCGCC 1 cut(s) 382
FaiI YATR 4 cut(s) 80, 246, 295, 332
FriOI GRGCYC 1 cut(s) 23
GlaI GCGC 1 cut(s) 382
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 1 cut(s) 249
HapII CCGG 2 cut(s) 167, 285
HhaI GCGC 1 cut(s) 383
Hin1I GRCGYC 1 cut(s) 381
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HinfI GANTC 3 cut(s) 225, 257, 281
HpaII CCGG 2 cut(s) 167, 285
HphI GGTGA 5 cut(s) 71, 95, 119, 290, 299
Hpy188I TCNGA 3 cut(s) 306, 347, 391
Hpy188III TCNNGA 1 cut(s) 173
HpyAV CCTTC 3 cut(s) 50, 134, 269
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 1 cut(s) 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 1 cut(s) 340
Hsp92I GRCGYC 1 cut(s) 381
HspAI GCGC 1 cut(s) 381
KasI GGCGCC 1 cut(s) 380
Kzo9I GATC 1 cut(s) 4
LpnPI CCDG 5 cut(s) 51, 122, 158, 180, 298
LweI GCATC 2 cut(s) 148, 280
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 2 cut(s) 230, 278
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 3 cut(s) 82, 281, 284
MhlI GDGCHC 1 cut(s) 23
Mly113I GGCGCC 1 cut(s) 381
MlyI GAGTC 2 cut(s) 266, 275
MmeI TCCRAC 2 cut(s) 193, 325
Mph1103I ATGCAT 1 cut(s) 295
MspI CCGG 2 cut(s) 167, 285
MspR9I CCNGG 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 34
NarI GGCGCC 1 cut(s) 381
NciI CCSGG 1 cut(s) 167
NdeII GATC 1 cut(s) 4
NlaIV GGNNCC 2 cut(s) 250, 382
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 295
PfeI GAWTC 1 cut(s) 225
PleI GAGTC 2 cut(s) 265, 275
PluTI GGCGCC 1 cut(s) 384
PpsI GAGTC 2 cut(s) 265, 275
Psp124BI GAGCTC 1 cut(s) 23
PspN4I GGNNCC 2 cut(s) 250, 382
PspPI GGNCC 1 cut(s) 248
SacI GAGCTC 1 cut(s) 23
Sau3AI GATC 1 cut(s) 4
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 2 cut(s) 266, 275
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 1 cut(s) 23
SetI ASST 9 cut(s) 23, 61, 85, 109, 205, 280, 331, 337, 343
SfaNI GCATC 2 cut(s) 148, 280
SfoI GGCGCC 1 cut(s) 382
SspDI GGCGCC 1 cut(s) 380
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 301
TaiI ACGT 1 cut(s) 343
TfiI GAWTC 1 cut(s) 225
TseFI GTSAC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 282
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.