RLG00000017724

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007208
Physical Location & Seq
Reverse (-)
20440435 .. 20444719
4285 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017724

Sequence Viewer

Length: 381 bp
ATGATATGGCATGGGTTTGTTGCTGCTGCTGTTGTTCCTGGAGGAGCATGCTTCATGCTTGCCTGGGTACGTACTTCTGATCAGTGCTGCATAAGTCCTACCGAGCACATGAACCAAGGCAGCATTGCATGGGAGGAGGCCAAGGTCTCGGTGGTCCCCTCTACCGCCTCTTTGGGATCTTCGTTTTGCTTGGAGCCGGATCGCGACCGTAAGATGTCAACTGCTTCAGGTTTGTTTTCTCTACCTTCTTCCTTCACACATCTCTCAGCCGGAGCTTCCTTCGTCTTTGATATTCACCGATCTGAGATTTGGATTTTCCCGATTCATATTTCGTGTCCTCAATCCTCGACCTCAAGTCCTCAAGTCCTCAATCCTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.61

Weight (kDa)

6.0

Isoelectric Point (pI)

67.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 204
AciI CCGC 1 cut(s) 165
AclWI GGATC 2 cut(s) 184, 207
AcuI CTGAAG 1 cut(s) 210
AfaI GTAC 2 cut(s) 69, 73
AhdI GACNNNNNGTC 1 cut(s) 354
AjnI CCWGG 2 cut(s) 37, 62
AluBI AGCT 1 cut(s) 275
AluI AGCT 1 cut(s) 275
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 2 cut(s) 184, 207
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 4 cut(s) 23, 26, 87, 120
AspS9I GGNCC 1 cut(s) 154
AsuHPI GGTGA 1 cut(s) 287
AvaII GGWCC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 4 cut(s) 10, 13, 74, 132
BciT130I CCWGG 2 cut(s) 39, 64
BclI TGATCA 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 1 cut(s) 379
BisI GCNGC 4 cut(s) 24, 27, 88, 121
BlsI GCNGC 4 cut(s) 25, 28, 89, 122
Bme1390I CCNGG 2 cut(s) 39, 64
Bme18I GGWCC 1 cut(s) 154
BmeRI GACNNNNNGTC 1 cut(s) 354
BmgT120I GGNCC 1 cut(s) 154
BmiI GGNNCC 2 cut(s) 156, 195
BmrFI CCNGG 2 cut(s) 39, 64
BpmI CTGGAG 1 cut(s) 60
BpuEI CTTGAG 2 cut(s) 337, 345
BsaAI YACGTR 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 63, 115, 141
Bse3DI GCAATG 1 cut(s) 123
BseBI CCWGG 2 cut(s) 39, 64
BseDI CCNNGG 3 cut(s) 63, 115, 141
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 2 cut(s) 279, 294
BseRI GAGGAG 2 cut(s) 57, 149
BseXI GCAGC 4 cut(s) 10, 13, 74, 132
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 1 cut(s) 140
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 2 cut(s) 197, 270
BslFI GGGAC 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 151
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 4 cut(s) 79, 176, 199, 299
Bsp68I TCGCGA 1 cut(s) 204
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 140
BspCNI CTCAG 2 cut(s) 278, 295
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 2 cut(s) 156, 195
BspPI GGATC 2 cut(s) 184, 207
BspTNI GGTCTC 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 123
BssECI CCNNGG 3 cut(s) 63, 115, 141
BssMI GATC 4 cut(s) 79, 176, 199, 299
BssT1I CCWWGG 2 cut(s) 115, 141
Bst2UI CCWGG 2 cut(s) 39, 64
Bst4CI ACNGT 1 cut(s) 209
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 49, 60
BstDEI CTNAG 2 cut(s) 265, 303
BstFNI CGCG 1 cut(s) 204
BstKTI GATC 4 cut(s) 82, 179, 202, 302
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 4 cut(s) 79, 176, 199, 299
BstMCI CGRYCG 1 cut(s) 208
BstNI CCWGG 2 cut(s) 39, 64
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 2 cut(s) 37, 62
BstSNI TACGTA 1 cut(s) 71
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 4 cut(s) 10, 13, 74, 132
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 1 cut(s) 140
BtsIMutI CAGTG 1 cut(s) 89
BtuMI TCGCGA 1 cut(s) 204
Cac8I GCNNGC 2 cut(s) 49, 60
Cfr13I GGNCC 1 cut(s) 154
Csp6I GTAC 2 cut(s) 68, 72
CviAII CATG 5 cut(s) 11, 48, 55, 109, 129
CviJI RGCY 4 cut(s) 140, 196, 269, 275
CviKI_1 RGCY 4 cut(s) 140, 196, 269, 275
CviQI GTAC 2 cut(s) 68, 72
DdeI CTNAG 2 cut(s) 265, 303
DpnI GATC 4 cut(s) 81, 178, 201, 301
DpnII GATC 4 cut(s) 79, 176, 199, 299
DriI GACNNNNNGTC 1 cut(s) 354
Eam1105I GACNNNNNGTC 1 cut(s) 354
Eco105I TACGTA 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 115, 141
Eco31I GGTCTC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 210
EcoRII CCWGG 2 cut(s) 37, 62
EcoT14I CCWWGG 2 cut(s) 115, 141
ErhI CCWWGG 2 cut(s) 115, 141
FaeI CATG 5 cut(s) 14, 51, 58, 112, 132
FaiI YATR 8 cut(s) 7, 12, 49, 56, 92, 110, 130, 327
FaqI GGGAC 1 cut(s) 140
FatI CATG 5 cut(s) 10, 47, 54, 108, 128
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 4 cut(s) 24, 27, 88, 121
Fsp4HI GCNGC 4 cut(s) 24, 27, 88, 121
FspBI CTAG 1 cut(s) 379
GluI GCNGC 4 cut(s) 24, 27, 88, 121
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 1 cut(s) 140
HapII CCGG 2 cut(s) 197, 270
Hin1II CATG 5 cut(s) 14, 51, 58, 112, 132
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HinfI GANTC 1 cut(s) 322
HpaII CCGG 2 cut(s) 197, 270
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 1 cut(s) 219
Hpy188I TCNGA 2 cut(s) 79, 304
Hpy188III TCNNGA 2 cut(s) 203, 319
Hpy8I GTNNAC 1 cut(s) 219
HpyAV CCTTC 3 cut(s) 255, 262, 289
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 2 cut(s) 90, 128
HpyF3I CTNAG 2 cut(s) 265, 303
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 5 cut(s) 14, 51, 58, 112, 132
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 4 cut(s) 79, 176, 199, 299
LmnI GCTCC 3 cut(s) 44, 193, 272
LpnPI CCDG 7 cut(s) 24, 49, 51, 76, 210, 213, 283
Lsp1109I GCAGC 4 cut(s) 10, 13, 74, 132
MaeI CTAG 1 cut(s) 379
MaeII ACGT 1 cut(s) 70
MalI GATC 4 cut(s) 81, 178, 201, 301
MboI GATC 4 cut(s) 79, 176, 199, 299
MboII GAAGA 2 cut(s) 171, 240
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 108
MspI CCGG 2 cut(s) 197, 270
MspR9I CCNGG 2 cut(s) 39, 64
MvaI CCWGG 2 cut(s) 39, 64
MvnI CGCG 1 cut(s) 204
NdeII GATC 4 cut(s) 79, 176, 199, 299
NlaIII CATG 5 cut(s) 14, 51, 58, 112, 132
NlaIV GGNNCC 2 cut(s) 156, 195
NruI TCGCGA 1 cut(s) 204
NspI RCATGY 1 cut(s) 51
PaeI GCATGC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 322
PfoI TCCNGGA 1 cut(s) 37
PkrI GCNGC 4 cut(s) 25, 28, 89, 122
Ppu21I YACGTR 1 cut(s) 71
Psp6I CCWGG 2 cut(s) 37, 62
PspGI CCWGG 2 cut(s) 37, 62
PspN4I GGNNCC 2 cut(s) 156, 195
PspPI GGNCC 1 cut(s) 154
PsuI RGATCY 1 cut(s) 176
RruI TCGCGA 1 cut(s) 204
RsaI GTAC 2 cut(s) 69, 73
RsaNI GTAC 2 cut(s) 68, 72
SatI GCNGC 4 cut(s) 24, 27, 88, 121
Sau3AI GATC 4 cut(s) 79, 176, 199, 299
Sau96I GGNCC 1 cut(s) 154
ScrFI CCNGG 2 cut(s) 39, 64
SduI GDGCHC 1 cut(s) 108
SetI ASST 6 cut(s) 73, 147, 232, 247, 277, 353
SinI GGWCC 1 cut(s) 154
SmlI CTYRAG 2 cut(s) 352, 360
SmoI CTYRAG 2 cut(s) 352, 360
SnaBI TACGTA 1 cut(s) 71
SphI GCATGC 1 cut(s) 51
SsiI CCGC 1 cut(s) 165
SspMI CTAG 1 cut(s) 379
StyD4I CCNGG 2 cut(s) 37, 62
StyI CCWWGG 2 cut(s) 115, 141
TaaI ACNGT 1 cut(s) 209
TaiI ACGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 347
TfiI GAWTC 1 cut(s) 322
TscAI CASTG 1 cut(s) 89
TseI GCWGC 4 cut(s) 23, 26, 87, 120
TspDTI ATGAA 3 cut(s) 43, 125, 314
TspRI CASTG 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 154
XceI RCATGY 1 cut(s) 51
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XspI CTAG 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.