RLG00000029664

sodium metabolite cotransporter BASS4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003648
Physical Location & Seq
Forward (+)
42509257 .. 42510759
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029664

Sequence Viewer

Length: 384 bp
ATGCAAGTTAGCAGGTCAAGGTCACTGCTGCTGAAGGTTAAGCCTGCAGTTTTTCTTGGGGCCGTTGGAATGGGAGTACTACTGCATCTCATCTTGTTAGCCTTTAATGCTGTTGCTGTTAAGAGCCTCTCAGCTGTGTCTGGTGGCAGTCAATCCGTTTTTTCCAAGAAGGAAAATGCAAATGCTGTTCTCCTTGTTGCAAGCCAGAAAACACTGCCTGTAATGGTGGCTGTGGTGGACCAACTAGGTGGTGCACTTGGTGAATCTGGTTTACTTGTTCTTCCTTGTGTTGCAGCACATTTAAATCAGATTATTCTGGACTCTATTCTTGTAAATTACTGGCTTCGCAAAGATCTCCCAACCAACAATGCCAAGGTATCATGA

Protein Analysis

128

Amino Acids

13.29

Weight (kDa)

9.92

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF_like PF13593 8 - 114 4.5e-09 SBF-like CPA transporter family (DUF4137)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 53
AdeI CACNNNGTG 1 cut(s) 260
AfaI GTAC 1 cut(s) 78
AluBI AGCT 1 cut(s) 134
AluI AGCT 1 cut(s) 134
Alw21I GWGCWC 1 cut(s) 256
Alw44I GTGCAC 1 cut(s) 252
AoxI GGCC 1 cut(s) 60
ApaLI GTGCAC 1 cut(s) 252
ApeKI GCWGC 2 cut(s) 28, 293
AspS9I GGNCC 2 cut(s) 60, 238
AsuHPI GGTGA 1 cut(s) 272
AvaII GGWCC 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 2 cut(s) 15, 305
BceAI ACGGC 1 cut(s) 47
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 1 cut(s) 45
BfuAI ACCTGC 1 cut(s) 3
BglII AGATCT 1 cut(s) 352
BisI GCNGC 2 cut(s) 29, 294
BlsI GCNGC 2 cut(s) 30, 295
BmcAI AGTACT 1 cut(s) 78
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 60, 238
BmiI GGNNCC 1 cut(s) 61
BmsI GCATC 1 cut(s) 94
BsaJI CCNNGG 1 cut(s) 372
Bse1I ACTGG 1 cut(s) 344
BseDI CCNNGG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 2 cut(s) 15, 305
BshFI GGCC 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 256
BsnI GGCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 1 cut(s) 352
BspANI GGCC 1 cut(s) 62
BspCNI CTCAG 1 cut(s) 143
BspHI TCATGA 1 cut(s) 380
BspLI GGNNCC 1 cut(s) 61
BspMAI CTGCAG 1 cut(s) 49
BspMI ACCTGC 1 cut(s) 3
BsrI ACTGG 1 cut(s) 344
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 372
BstC8I GCNNGC 2 cut(s) 45, 202
BstDEI CTNAG 1 cut(s) 130
BstKTI GATC 1 cut(s) 355
BstMBI GATC 1 cut(s) 352
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 45
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 2 cut(s) 15, 305
BstX2I RGATCY 1 cut(s) 352
BstXI CCANNNNNNTGG 1 cut(s) 248
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 1 cut(s) 62
BtsI GCAGTG 2 cut(s) 23, 212
BtsIMutI CAGTG 2 cut(s) 23, 212
BveI ACCTGC 1 cut(s) 3
Cac8I GCNNGC 2 cut(s) 45, 202
CciI TCATGA 1 cut(s) 380
Cfr13I GGNCC 2 cut(s) 60, 238
Csp6I GTAC 1 cut(s) 77
CviAII CATG 1 cut(s) 381
CviJI RGCY 8 cut(s) 43, 62, 101, 126, 134, 204, 230, 343
CviKI_1 RGCY 8 cut(s) 43, 62, 101, 126, 134, 204, 230, 343
CviQI GTAC 1 cut(s) 77
DdeI CTNAG 1 cut(s) 130
DpnI GATC 1 cut(s) 354
DpnII GATC 1 cut(s) 352
DraI TTTAAA 1 cut(s) 303
DraIII CACNNNGTG 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 53
EcoT14I CCWWGG 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 1 cut(s) 384
FaiI YATR 1 cut(s) 382
FatI CATG 1 cut(s) 380
Fnu4HI GCNGC 2 cut(s) 29, 294
Fsp4HI GCNGC 2 cut(s) 29, 294
FspBI CTAG 1 cut(s) 245
GluI GCNGC 2 cut(s) 29, 294
HaeIII GGCC 1 cut(s) 62
Hin1II CATG 1 cut(s) 384
HinfI GANTC 2 cut(s) 263, 320
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 3 cut(s) 238, 254, 272
Hpy188I TCNGA 1 cut(s) 309
Hpy188III TCNNGA 2 cut(s) 317, 381
Hpy8I GTNNAC 3 cut(s) 238, 254, 272
HpyAV CCTTC 2 cut(s) 28, 163
HpyCH4V TGCA 7 cut(s) 4, 47, 85, 179, 200, 254, 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 1 cut(s) 130
Hsp92II CATG 1 cut(s) 384
Kzo9I GATC 1 cut(s) 352
LpnPI CCDG 7 cut(s) 57, 126, 218, 231, 252, 302, 325
Lsp1109I GCAGC 2 cut(s) 15, 305
LweI GCATC 1 cut(s) 94
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 1 cut(s) 21
MalI GATC 1 cut(s) 354
MboI GATC 1 cut(s) 352
MboII GAAGA 1 cut(s) 272
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 1 cut(s) 334
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 1 cut(s) 137
MseI TTAA 4 cut(s) 39, 105, 120, 302
MspA1I CMGCKG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeII GATC 1 cut(s) 352
NlaIII CATG 1 cut(s) 384
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 21
PagI TCATGA 1 cut(s) 380
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 2 cut(s) 30, 295
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 2 cut(s) 60, 238
PstI CTGCAG 1 cut(s) 49
PsuI RGATCY 1 cut(s) 352
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 1 cut(s) 78
RsaNI GTAC 1 cut(s) 77
SaqAI TTAA 4 cut(s) 39, 105, 120, 302
SatI GCNGC 2 cut(s) 29, 294
Sau3AI GATC 1 cut(s) 352
Sau96I GGNCC 2 cut(s) 60, 238
ScaI AGTACT 1 cut(s) 78
SchI GAGTC 1 cut(s) 314
SduI GDGCHC 1 cut(s) 256
SetI ASST 6 cut(s) 17, 23, 39, 136, 250, 378
SfaNI GCATC 1 cut(s) 94
SfcI CTRYAG 1 cut(s) 45
SinI GGWCC 1 cut(s) 238
SmiI ATTTAAAT 1 cut(s) 303
Sse9I AATT 1 cut(s) 334
SspMI CTAG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 372
SwaI ATTTAAAT 1 cut(s) 303
TasI AATT 1 cut(s) 334
TatI WGTACW 1 cut(s) 76
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 4 cut(s) 39, 105, 120, 302
Tru9I TTAA 4 cut(s) 39, 105, 120, 302
TscAI CASTG 2 cut(s) 30, 219
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 2 cut(s) 28, 293
Tsp45I GTSAC 1 cut(s) 21
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 2 cut(s) 30, 219
VneI GTGCAC 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 238
XspI CTAG 1 cut(s) 245
ZrmI AGTACT 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.