Rorug01G0101700

sodium metabolite cotransporter BASS4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003648
Physical Location & Seq
Reverse (-)
16907103 .. 16907846
744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0101700.1

Sequence Viewer

Length: 744 bp
ATGGAGCTACAAACCCAACAACAAATCGAGGATGGAGGAAACAACCAAGCAGCCGGGAGTAGTGCCAACACGGATTTATGGCGAAGCCGTACCAGGTGGATTCCTCCTCCAGATCAAATAAGAATCCTCAAGGATCTTTACTACGACAAGGGAGTTAAGTGCCCAACTACAGAGCACATTCACGAGATCTGTCTCCAGCTGAACCAGTATGGACATGTTGAGGGCAAGAACATTTATTTTTGGTTCCAGAATGTCAGGGCTCGAGAGAAGCAGATGAAGAGGTGCAATCAGGCTGCTCAAGTGCCCGTGGGAACTAGTTCTACTGGTACTGGTGGATCCATTGATATTAACTTTGGGTCCACTAGTCCTACTGGTGCTGGTGTATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGCCACGGGTCGACTGCAGTGTTGACCGGTATTCCTCCTCACCCTTCAACACTAATACTAGTACTTCCTCCTTTGGCACAACAGGAGGACAAACTTTCATGGAACAGCGAGGGGAGGATCACCAGGAGATTGAAACTCTTCCTCTGTTCCCCATGCACGGTGAAGACGTCCTTGGTGGCCTGAAGACTACTTCTGAGGGAGGTAGCGGCTATGGCTACTATTCCGGTGGCTCGGGAAGTTACAACGGTGGCTCTCACGTTTCTCTTGAGCTCAGCCTCAACCCATCCGGAGCTGGTGACTTGGCTTAG

Protein Analysis

247

Amino Acids

26.5

Weight (kDa)

5.07

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 30 - 91 3.8e-08 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 606
AccB7I CCANNNNNTGG 1 cut(s) 413
AccI GTMKAC 1 cut(s) 447
AccIII TCCGGA 1 cut(s) 722
AciI CCGC 1 cut(s) 642
AclWI GGATC 6 cut(s) 141, 330, 343, 411, 424, 561
AcuI CTGAAG 1 cut(s) 638
AcyI GRCGYC 1 cut(s) 603
AfaI GTAC 3 cut(s) 91, 328, 499
AfiI CCNNNNNNNGG 2 cut(s) 413, 593
AflIII ACRYGT 1 cut(s) 214
AgeI ACCGGT 1 cut(s) 462
AgsI TTSAA 2 cut(s) 484, 569
AhlI ACTAGT 3 cut(s) 314, 362, 494
AjnI CCWGG 2 cut(s) 92, 558
AjuI GAANNNNNNNTTGG 2 cut(s) 591, 623
AluBI AGCT 5 cut(s) 7, 199, 410, 706, 728
AluI AGCT 5 cut(s) 7, 199, 410, 706, 728
Alw21I GWGCWC 2 cut(s) 177, 708
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 6 cut(s) 141, 330, 343, 411, 424, 561
Ama87I CYCGRG 2 cut(s) 261, 667
Aor13HI TCCGGA 1 cut(s) 722
AoxI GGCC 3 cut(s) 404, 437, 613
ApeKI GCWGC 2 cut(s) 50, 293
ArsI GACNNNNNNTTYG 4 cut(s) 383, 415, 416, 448
AsiGI ACCGGT 1 cut(s) 462
Asp700I GAANNNNTTC 2 cut(s) 316, 573
AspS9I GGNCC 3 cut(s) 357, 404, 437
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 4 cut(s) 468, 548, 608, 743
AvaI CYCGRG 2 cut(s) 261, 667
AvaII GGWCC 1 cut(s) 357
BaeGI GKGCMC 2 cut(s) 164, 306
BamHI GGATCC 2 cut(s) 335, 416
BanII GRGCYC 2 cut(s) 262, 708
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 2 cut(s) 606, 626
Bbv12I GWGCWC 2 cut(s) 177, 708
BbvI GCAGC 2 cut(s) 62, 280
BccI CCATC 3 cut(s) 26, 395, 727
BceAI ACGGC 1 cut(s) 72
BciT130I CCWGG 2 cut(s) 94, 560
BciVI GTATCC 1 cut(s) 394
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 3 cut(s) 314, 362, 494
BfaI CTAG 3 cut(s) 315, 363, 495
BfmI CTRYAG 2 cut(s) 168, 451
BfuI GTATCC 1 cut(s) 394
BglII AGATCT 1 cut(s) 186
BisI GCNGC 3 cut(s) 51, 294, 643
BlpI GCTNAGC 1 cut(s) 707
BlsI GCNGC 3 cut(s) 52, 295, 644
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 3 cut(s) 55, 94, 560
Bme18I GGWCC 1 cut(s) 357
BmeT110I CYCGRG 2 cut(s) 261, 667
BmgT120I GGNCC 3 cut(s) 357, 404, 437
BmiI GGNNCC 4 cut(s) 245, 337, 358, 418
BmrFI CCNGG 3 cut(s) 55, 94, 560
BpiI GAAGAC 2 cut(s) 606, 626
BpmI CTGGAG 2 cut(s) 93, 179
Bpu1102I GCTNAGC 1 cut(s) 707
BpuEI CTTGAG 3 cut(s) 113, 282, 722
BpuMI CCSGG 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 603
BsaJI CCNNGG 3 cut(s) 306, 440, 607
BsaWI WCCGGW 3 cut(s) 462, 659, 722
Bsc4I CCNNNNNNNGG 2 cut(s) 413, 593
Bse118I RCCGGY 1 cut(s) 462
Bse1I ACTGG 4 cut(s) 205, 328, 334, 376
BseAI TCCGGA 1 cut(s) 722
BseBI CCWGG 2 cut(s) 94, 560
BseDI CCNNGG 3 cut(s) 306, 440, 607
BseGI GGATG 2 cut(s) 37, 719
BseLI CCNNNNNNNGG 2 cut(s) 413, 593
BseMII CTCAG 2 cut(s) 621, 721
BseNI ACTGG 4 cut(s) 205, 328, 334, 376
BseRI GAGGAG 2 cut(s) 96, 463
BseSI GKGCMC 2 cut(s) 164, 306
BseXI GCAGC 2 cut(s) 62, 280
BshFI GGCC 3 cut(s) 406, 439, 615
BshTI ACCGGT 1 cut(s) 462
BsiHKAI GWGCWC 2 cut(s) 177, 708
BsiHKCI CYCGRG 2 cut(s) 261, 667
BsiSI CCGG 4 cut(s) 54, 463, 660, 723
BslI CCNNNNNNNGG 2 cut(s) 413, 593
BsmAI GTCTC 1 cut(s) 197
BsnI GGCC 3 cut(s) 406, 439, 615
BsoBI CYCGRG 2 cut(s) 261, 667
Bsp1286I GDGCHC 5 cut(s) 164, 177, 262, 306, 708
Bsp13I TCCGGA 1 cut(s) 722
Bsp143I GATC 6 cut(s) 112, 133, 186, 335, 416, 553
Bsp1720I GCTNAGC 1 cut(s) 707
BspACI CCGC 1 cut(s) 642
BspANI GGCC 3 cut(s) 406, 439, 615
BspCNI CTCAG 2 cut(s) 622, 720
BspEI TCCGGA 1 cut(s) 722
BspLI GGNNCC 4 cut(s) 245, 337, 358, 418
BspMAI CTGCAG 1 cut(s) 455
BspPI GGATC 6 cut(s) 141, 330, 343, 411, 424, 561
BsrFI RCCGGY 1 cut(s) 462
BsrI ACTGG 4 cut(s) 205, 328, 334, 376
BssAI RCCGGY 1 cut(s) 462
BssECI CCNNGG 3 cut(s) 306, 440, 607
BssMI GATC 6 cut(s) 112, 133, 186, 335, 416, 553
BssNI GRCGYC 1 cut(s) 603
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 1 cut(s) 607
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 2 cut(s) 94, 560
Bst4CI ACNGT 2 cut(s) 596, 683
Bst6I CTCTTC 2 cut(s) 272, 579
BstACI GRCGYC 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 3 cut(s) 630, 707, 741
BstDSI CCRYGG 2 cut(s) 306, 440
BstF5I GGATG 2 cut(s) 37, 719
BstKTI GATC 6 cut(s) 115, 136, 189, 338, 419, 556
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 6 cut(s) 112, 133, 186, 335, 416, 553
BstMWI GCNNNNNNNGC 1 cut(s) 648
BstNI CCWGG 2 cut(s) 94, 560
BstNSI RCATGY 1 cut(s) 218
BstSCI CCNGG 3 cut(s) 53, 92, 558
BstSFI CTRYAG 2 cut(s) 168, 451
BstSLI GKGCMC 2 cut(s) 164, 306
BstV1I GCAGC 2 cut(s) 62, 280
BstV2I GAAGAC 2 cut(s) 606, 626
BstX2I RGATCY 4 cut(s) 133, 186, 335, 416
BstYI RGATCY 4 cut(s) 133, 186, 335, 416
BsuI GTATCC 1 cut(s) 394
BsuRI GGCC 3 cut(s) 406, 439, 615
BtgI CCRYGG 2 cut(s) 306, 440
BtsCI GGATG 2 cut(s) 37, 719
BtsI GCAGTG 1 cut(s) 460
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 408
Cfr10I RCCGGY 1 cut(s) 462
Cfr13I GGNCC 3 cut(s) 357, 404, 437
CsiI ACCWGGT 1 cut(s) 92
Csp6I GTAC 3 cut(s) 90, 327, 498
CspAI ACCGGT 1 cut(s) 462
CviAII CATG 3 cut(s) 215, 535, 589
CviQI GTAC 3 cut(s) 90, 327, 498
DdeI CTNAG 3 cut(s) 630, 707, 741
DpnI GATC 6 cut(s) 114, 135, 188, 337, 418, 555
DpnII GATC 6 cut(s) 112, 133, 186, 335, 416, 553
Eam1104I CTCTTC 2 cut(s) 272, 579
EarI CTCTTC 2 cut(s) 272, 579
Ecl136II GAGCTC 1 cut(s) 706
Eco130I CCWWGG 1 cut(s) 607
Eco24I GRGCYC 2 cut(s) 262, 708
Eco47I GGWCC 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 706
Eco57I CTGAAG 1 cut(s) 638
Eco88I CYCGRG 2 cut(s) 261, 667
EcoICRI GAGCTC 1 cut(s) 706
EcoRII CCWGG 2 cut(s) 92, 558
EcoT14I CCWWGG 1 cut(s) 607
EcoT38I GRGCYC 2 cut(s) 262, 708
ErhI CCWWGG 1 cut(s) 607
FaeI CATG 3 cut(s) 218, 538, 592
FaiI YATR 6 cut(s) 79, 210, 216, 536, 590, 648
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FatI CATG 3 cut(s) 214, 534, 588
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 3 cut(s) 51, 294, 643
FokI GGATG 2 cut(s) 44, 706
FriOI GRGCYC 2 cut(s) 262, 708
Fsp4HI GCNGC 3 cut(s) 51, 294, 643
FspBI CTAG 3 cut(s) 315, 363, 495
GluI GCNGC 3 cut(s) 51, 294, 643
GsuI CTGGAG 2 cut(s) 93, 179
HaeIII GGCC 3 cut(s) 406, 439, 615
HapII CCGG 4 cut(s) 54, 463, 660, 723
Hin1I GRCGYC 1 cut(s) 603
Hin1II CATG 3 cut(s) 218, 538, 592
HincII GTYRAC 2 cut(s) 448, 460
HindII GTYRAC 2 cut(s) 448, 460
HinfI GANTC 2 cut(s) 100, 123
HpaII CCGG 4 cut(s) 54, 463, 660, 723
HphI GGTGA 4 cut(s) 468, 548, 608, 743
Hpy166II GTNNAC 3 cut(s) 360, 448, 460
Hpy188I TCNGA 1 cut(s) 631
Hpy188III TCNNGA 7 cut(s) 110, 182, 247, 263, 669, 701, 723
Hpy8I GTNNAC 3 cut(s) 360, 448, 460
HpyAV CCTTC 1 cut(s) 490
HpyCH4III ACNGT 2 cut(s) 596, 683
HpyCH4IV ACGT 2 cut(s) 603, 693
HpyCH4V TGCA 3 cut(s) 285, 453, 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 648
HpyF3I CTNAG 3 cut(s) 630, 707, 741
HpySE526I ACGT 2 cut(s) 603, 693
Hsp92I GRCGYC 1 cut(s) 603
Hsp92II CATG 3 cut(s) 218, 538, 592
Kpn2I TCCGGA 1 cut(s) 722
Kzo9I GATC 6 cut(s) 112, 133, 186, 335, 416, 553
LmnI GCTCC 2 cut(s) 4, 725
Lsp1109I GCAGC 2 cut(s) 62, 280
MabI ACCWGGT 1 cut(s) 92
MaeI CTAG 3 cut(s) 315, 363, 495
MaeII ACGT 2 cut(s) 603, 693
MaeIII GTNAC 2 cut(s) 674, 731
MalI GATC 6 cut(s) 114, 135, 188, 337, 418, 555
MboI GATC 6 cut(s) 112, 133, 186, 335, 416, 553
MboII GAAGA 4 cut(s) 289, 566, 611, 631
MflI RGATCY 4 cut(s) 133, 186, 335, 416
MhlI GDGCHC 5 cut(s) 164, 177, 262, 306, 708
MluCI AATT 2 cut(s) 397, 430
MroI TCCGGA 1 cut(s) 722
MroXI GAANNNNTTC 2 cut(s) 316, 573
MseI TTAA 2 cut(s) 156, 348
MspA1I CMGCKG 2 cut(s) 199, 410
MspI CCGG 4 cut(s) 54, 463, 660, 723
MspR9I CCNGG 3 cut(s) 55, 94, 560
MvaI CCWGG 2 cut(s) 94, 560
MwoI GCNNNNNNNGC 1 cut(s) 648
NciI CCSGG 1 cut(s) 55
NdeII GATC 6 cut(s) 112, 133, 186, 335, 416, 553
NlaIII CATG 3 cut(s) 218, 538, 592
NlaIV GGNNCC 4 cut(s) 245, 337, 358, 418
NmuCI GTSAC 1 cut(s) 731
NspI RCATGY 1 cut(s) 218
PaeR7I CTCGAG 1 cut(s) 261
PciI ACATGT 1 cut(s) 214
PcsI WCGNNNNNNNCGW 1 cut(s) 600
PdmI GAANNNNTTC 2 cut(s) 316, 573
PfeI GAWTC 2 cut(s) 100, 123
PflMI CCANNNNNTGG 1 cut(s) 413
PinAI ACCGGT 1 cut(s) 462
PkrI GCNGC 3 cut(s) 52, 295, 644
PscI ACATGT 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 708
Psp6I CCWGG 2 cut(s) 92, 558
PspGI CCWGG 2 cut(s) 92, 558
PspN4I GGNNCC 4 cut(s) 245, 337, 358, 418
PspPI GGNCC 3 cut(s) 357, 404, 437
PsrI GAACNNNNNNTAC 2 cut(s) 304, 336
PstI CTGCAG 1 cut(s) 455
PsuI RGATCY 4 cut(s) 133, 186, 335, 416
PvuII CAGCTG 2 cut(s) 199, 410
RsaI GTAC 3 cut(s) 91, 328, 499
RsaNI GTAC 3 cut(s) 90, 327, 498
SacI GAGCTC 1 cut(s) 708
SalI GTCGAC 1 cut(s) 446
SaqAI TTAA 2 cut(s) 156, 348
SatI GCNGC 3 cut(s) 51, 294, 643
Sau3AI GATC 6 cut(s) 112, 133, 186, 335, 416, 553
Sau96I GGNCC 3 cut(s) 357, 404, 437
ScaI AGTACT 1 cut(s) 499
ScrFI CCNGG 3 cut(s) 55, 94, 560
SduI GDGCHC 5 cut(s) 164, 177, 262, 306, 708
SexAI ACCWGGT 1 cut(s) 92
SfcI CTRYAG 2 cut(s) 168, 451
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 357
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 4 cut(s) 128, 261, 297, 701
SmoI CTYRAG 4 cut(s) 128, 261, 297, 701
SpeI ACTAGT 3 cut(s) 314, 362, 494
Sse9I AATT 2 cut(s) 397, 430
SsiI CCGC 1 cut(s) 642
SspMI CTAG 3 cut(s) 315, 363, 495
SstI GAGCTC 1 cut(s) 708
StyD4I CCNGG 3 cut(s) 53, 92, 558
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 2 cut(s) 596, 683
TaiI ACGT 2 cut(s) 606, 696
TaqI TCGA 4 cut(s) 27, 262, 390, 447
TasI AATT 2 cut(s) 397, 430
TatI WGTACW 1 cut(s) 497
TauI GCSGC 1 cut(s) 645
TfiI GAWTC 2 cut(s) 100, 123
Tru1I TTAA 2 cut(s) 156, 348
Tru9I TTAA 2 cut(s) 156, 348
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 1 cut(s) 731
TseI GCWGC 2 cut(s) 50, 293
Tsp45I GTSAC 1 cut(s) 731
TspDTI ATGAA 2 cut(s) 290, 523
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 1 cut(s) 460
Van91I CCANNNNNTGG 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 357
XceI RCATGY 1 cut(s) 218
XhoI CTCGAG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 447
XmnI GAANNNNTTC 2 cut(s) 316, 573
XspI CTAG 3 cut(s) 315, 363, 495
ZraI GACGTC 1 cut(s) 604
ZrmI AGTACT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.