RLG00000033670

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010630
Physical Location & Seq
Reverse (-)
30558335 .. 30564388
6054 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033670

Sequence Viewer

Length: 1065 bp
ATGAACGTCTTGCTGAGTGGGGCGGAAGACTATCTTCTTCTAGCAGGATCTGAACTTCTTAGGGCTGTGGCTGTTGGTGAGAAAGCCGCCTGTGCACCAGAGGGTATTCGTGGCTGGAGTGTAGCAGAGGGAGTTGGTGTTGACGAACATGAAGCGAAGTTTGCTCTGGGCCATGGTGGTGATGAATTGGACTGCGCTGCGAAGGCAGATGCGGTTCCTGTCGGAGTCAATGATGGCCATGCCGTTGCGGTGGCCGTAGATTTGTCGGATACCCATGCTGATGGTGTCGCCGCTGCTGACCGTCGTCTTTTCGGAACCACAGAAGAGCCGCATGGGGATGAATCTAACAACGATGAGGATGACGCCGATAGGTTCAAACCTTACACACCGTCAAGCTCGCCGCAATTCCTTTACCGCCACCCGTCTTCTTCTTTCCGCTCCGTTTTCAAACCATTATCCAAAACCTTCCTCTACTGCCATAGCCACATACCAAGACCTCAAGTTCCTCTCTTCTTCGACATAGCACCAAAAGCAACAAGCCCAGATGAGAAAGGTATGAAACCGCAGTGGAAAGCGATAGATTTCAAGTGGATAAGGGAGAACAAGGATGTTGTTGCTTGGGTAGGGCTTGAAAGGAGTTCTGTTGGACATTGGTGGCTGGATAATATAGGAAAGGCTTTTGGAGAAGGAAAAACTTATGAATGTATGGGTTCTAGGCAGCTAGCAGGCTTGTTCATAACTCTTTGTTCCATGCAGCTTTGTCGTGCCATTGGTAACAAGGTTACAACTTCAAGATTATTATTCTCAAAGCAAACTATTCCTCATTATTATTTGACAGTAGATACATGTGTCGACAAGCTCGTTGAGTTGAGAGGCCAACTCAATTCATTGCAGGAAGCTACAGGTGGGAAGCGGATATCTATCAATGATCTTGTAATAAAGGCTGCTGCTTTGGCTCTTCAAAAGGTTCCTCAGGCTGCTGCTGCTTTGGCTCTTCAAATGGAATTTATGAGGGATATGCAGCTCAGGAGTTTGCCAAGTAGGGGGTTAAGCCAGGCGAACTAG

Protein Analysis

355

Amino Acids

38.56

Weight (kDa)

5.95

Isoelectric Point (pI)

38.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 260 - 330 4.1e-13 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 438
AccI GTMKAC 1 cut(s) 852
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 2 cut(s) 235, 252
AcsI RAATTY 1 cut(s) 1004
AcyI GRCGYC 1 cut(s) 363
AfiI CCNNNNNNNGG 1 cut(s) 1043
AflIII ACRYGT 1 cut(s) 845
AgsI TTSAA 7 cut(s) 376, 448, 586, 632, 792, 962, 998
AjnI CCWGG 1 cut(s) 1053
AluBI AGCT 6 cut(s) 396, 721, 757, 859, 899, 1024
AluI AGCT 6 cut(s) 396, 721, 757, 859, 899, 1024
Alw21I GWGCWC 1 cut(s) 97
Alw44I GTGCAC 1 cut(s) 93
AlwI GGATC 1 cut(s) 55
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 4 cut(s) 169, 235, 252, 874
ApaLI GTGCAC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 1004
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 2 cut(s) 89, 191
AsuNHI GCTAGC 1 cut(s) 721
AxyI CCTNAGG 1 cut(s) 972
BaeGI GKGCMC 1 cut(s) 97
BaeI ACNNNNGTAYC 2 cut(s) 834, 867
BalI TGGCCA 1 cut(s) 237
BbsI GAAGAC 2 cut(s) 33, 417
Bbv12I GWGCWC 1 cut(s) 97
BccI CCATC 2 cut(s) 227, 275
BceAI ACGGC 2 cut(s) 227, 239
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 1 cut(s) 1055
BciVI GTATCC 1 cut(s) 262
BfaI CTAG 4 cut(s) 41, 714, 722, 1063
BfmI CTRYAG 1 cut(s) 900
BfuI GTATCC 1 cut(s) 262
Bme1390I CCNGG 1 cut(s) 1055
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 3 cut(s) 216, 316, 969
BmrFI CCNGG 1 cut(s) 1055
BmsI GCATC 1 cut(s) 199
BmtI GCTAGC 1 cut(s) 725
BoxI GACNNNNGTC 1 cut(s) 303
BpiI GAAGAC 2 cut(s) 33, 417
BplI GAGNNNNNCTC 2 cut(s) 864, 896
BpmI CTGGAG 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 1025
BpuEI CTTGAG 1 cut(s) 483
BsaBI GATNNNNATC 1 cut(s) 920
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 1 cut(s) 172
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 1 cut(s) 1043
Bse21I CCTNAGG 1 cut(s) 972
Bse3DI GCAATG 1 cut(s) 887
Bse8I GATNNNNATC 1 cut(s) 920
BseBI CCWGG 1 cut(s) 1055
BseDI CCNNGG 1 cut(s) 172
BseGI GGATG 3 cut(s) 343, 364, 613
BseJI GATNNNNATC 1 cut(s) 920
BseLI CCNNNNNNNGG 1 cut(s) 1043
BseMI GCAATG 1 cut(s) 887
BseMII CTCAG 3 cut(s) 5, 986, 1039
BseSI GKGCMC 1 cut(s) 97
BshFI GGCC 4 cut(s) 171, 237, 254, 876
BsiHKAI GWGCWC 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 1043
BsnI GGCC 4 cut(s) 171, 237, 254, 876
Bsp1286I GDGCHC 1 cut(s) 97
Bsp143I GATC 2 cut(s) 47, 928
Bsp19I CCATGG 1 cut(s) 172
BspANI GGCC 4 cut(s) 171, 237, 254, 876
BspCNI CTCAG 3 cut(s) 6, 985, 1038
BspLI GGNNCC 3 cut(s) 216, 316, 969
BspOI GCTAGC 1 cut(s) 725
BspPI GGATC 1 cut(s) 55
BspQI GCTCTTC 3 cut(s) 318, 963, 999
BsrBI CCGCTC 1 cut(s) 438
BsrDI GCAATG 1 cut(s) 887
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 2 cut(s) 47, 928
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 172
Bst2UI CCWGG 1 cut(s) 1055
Bst4CI ACNGT 3 cut(s) 302, 390, 838
Bst6I CTCTTC 4 cut(s) 318, 515, 963, 999
BstACI GRCGYC 1 cut(s) 363
BstC8I GCNNGC 3 cut(s) 398, 723, 727
BstDEI CTNAG 4 cut(s) 14, 59, 972, 1025
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 3 cut(s) 343, 364, 613
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 2 cut(s) 50, 931
BstMBI GATC 2 cut(s) 47, 928
BstMWI GCNNNNNNNGC 7 cut(s) 92, 161, 203, 530, 953, 983, 989
BstNI CCWGG 1 cut(s) 1055
BstNSI RCATGY 1 cut(s) 849
BstPAI GACNNNNGTC 1 cut(s) 303
BstSCI CCNGG 1 cut(s) 1053
BstSFI CTRYAG 1 cut(s) 900
BstSLI GKGCMC 1 cut(s) 97
BstV2I GAAGAC 2 cut(s) 33, 417
BstX2I RGATCY 1 cut(s) 47
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 1 cut(s) 47
Bsu36I CCTNAGG 1 cut(s) 972
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 4 cut(s) 171, 237, 254, 876
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 3 cut(s) 343, 364, 613
BtsI GCAGTG 1 cut(s) 572
BtsIMutI CAGTG 1 cut(s) 572
Cac8I GCNNGC 3 cut(s) 398, 723, 727
CaiI CAGNNNCTG 1 cut(s) 50
CfoI GCGC 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 169
CseI GACGC 1 cut(s) 371
CviAII CATG 7 cut(s) 149, 173, 239, 275, 332, 751, 846
DdeI CTNAG 4 cut(s) 14, 59, 972, 1025
DpnI GATC 2 cut(s) 49, 930
DpnII GATC 2 cut(s) 47, 928
EaeI YGGCCR 2 cut(s) 235, 252
Eam1104I CTCTTC 4 cut(s) 318, 515, 963, 999
EarI CTCTTC 4 cut(s) 318, 515, 963, 999
EciI GGCGGA 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 172
Eco32I GATATC 1 cut(s) 918
Eco81I CCTNAGG 1 cut(s) 972
EcoRII CCWGG 1 cut(s) 1053
EcoRV GATATC 1 cut(s) 918
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 7 cut(s) 152, 176, 242, 278, 335, 754, 849
FalI AAGNNNNNCTT 2 cut(s) 18, 50
FatI CATG 7 cut(s) 148, 172, 238, 274, 331, 750, 845
FblI GTMKAC 1 cut(s) 852
FokI GGATG 3 cut(s) 350, 371, 620
FspBI CTAG 4 cut(s) 41, 714, 722, 1063
GlaI GCGC 1 cut(s) 196
GsuI CTGGAG 1 cut(s) 136
HaeIII GGCC 4 cut(s) 171, 237, 254, 876
HgaI GACGC 1 cut(s) 371
HhaI GCGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 7 cut(s) 152, 176, 242, 278, 335, 754, 849
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HincII GTYRAC 2 cut(s) 142, 853
HindII GTYRAC 2 cut(s) 142, 853
HinfI GANTC 2 cut(s) 225, 341
HphI GGTGA 2 cut(s) 89, 191
Hpy166II GTNNAC 3 cut(s) 95, 142, 853
Hpy188I TCNGA 4 cut(s) 52, 224, 268, 314
Hpy188III TCNNGA 2 cut(s) 792, 1027
Hpy8I GTNNAC 3 cut(s) 95, 142, 853
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 3 cut(s) 196, 475, 680
HpyCH4III ACNGT 3 cut(s) 302, 390, 838
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 4 cut(s) 95, 754, 892, 1021
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 161, 203, 530, 953, 983, 989
HpyF3I CTNAG 4 cut(s) 14, 59, 972, 1025
HpySE526I ACGT 1 cut(s) 6
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 7 cut(s) 152, 176, 242, 278, 335, 754, 849
HspAI GCGC 1 cut(s) 195
Kzo9I GATC 2 cut(s) 47, 928
LguI GCTCTTC 3 cut(s) 318, 963, 999
LmnI GCTCC 1 cut(s) 443
LweI GCATC 1 cut(s) 199
MaeI CTAG 4 cut(s) 41, 714, 722, 1063
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 2 cut(s) 773, 781
MalI GATC 2 cut(s) 49, 930
MbiI CCGCTC 1 cut(s) 438
MboI GATC 2 cut(s) 47, 928
MflI RGATCY 1 cut(s) 47
MhlI GDGCHC 1 cut(s) 97
MlsI TGGCCA 1 cut(s) 237
MluCI AATT 4 cut(s) 185, 404, 883, 1004
MluNI TGGCCA 1 cut(s) 237
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 3 cut(s) 202, 246, 625
Mox20I TGGCCA 1 cut(s) 237
MscI TGGCCA 1 cut(s) 237
MseI TTAA 1 cut(s) 1049
MslI CAYNNNNRTG 3 cut(s) 177, 279, 336
Msp20I TGGCCA 1 cut(s) 237
MspA1I CMGCKG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 1055
MvaI CCWGG 1 cut(s) 1055
MwoI GCNNNNNNNGC 7 cut(s) 92, 161, 203, 530, 953, 983, 989
NcoI CCATGG 1 cut(s) 172
NdeII GATC 2 cut(s) 47, 928
NheI GCTAGC 1 cut(s) 721
NlaIII CATG 7 cut(s) 152, 176, 242, 278, 335, 754, 849
NlaIV GGNNCC 3 cut(s) 216, 316, 969
NspI RCATGY 1 cut(s) 849
PciI ACATGT 1 cut(s) 845
PciSI GCTCTTC 3 cut(s) 318, 963, 999
PcsI WCGNNNNNNNCGW 1 cut(s) 858
PfeI GAWTC 1 cut(s) 341
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PscI ACATGT 1 cut(s) 845
PshAI GACNNNNGTC 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 1053
PspGI CCWGG 1 cut(s) 1053
PspN4I GGNNCC 3 cut(s) 216, 316, 969
PspPI GGNCC 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 47
RseI CAYNNNNRTG 3 cut(s) 177, 279, 336
SalI GTCGAC 1 cut(s) 851
SapI GCTCTTC 3 cut(s) 318, 963, 999
SaqAI TTAA 1 cut(s) 1049
Sau3AI GATC 2 cut(s) 47, 928
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 1 cut(s) 234
ScrFI CCNGG 1 cut(s) 1055
SduI GDGCHC 1 cut(s) 97
SfaNI GCATC 1 cut(s) 199
SfcI CTRYAG 1 cut(s) 900
SmiMI CAYNNNNRTG 3 cut(s) 177, 279, 336
SmlI CTYRAG 1 cut(s) 498
SmoI CTYRAG 1 cut(s) 498
Sse9I AATT 4 cut(s) 185, 404, 883, 1004
SspMI CTAG 4 cut(s) 41, 714, 722, 1063
StyD4I CCNGG 1 cut(s) 1053
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 3 cut(s) 302, 390, 838
TaiI ACGT 1 cut(s) 9
TaqI TCGA 2 cut(s) 516, 852
TasI AATT 4 cut(s) 185, 404, 883, 1004
TauI GCSGC 4 cut(s) 89, 293, 331, 403
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 1 cut(s) 1049
Tru9I TTAA 1 cut(s) 1049
TscAI CASTG 1 cut(s) 572
TspDTI ATGAA 8 cut(s) 17, 165, 198, 354, 572, 714, 724, 876
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 1 cut(s) 572
VneI GTGCAC 1 cut(s) 93
XapI RAATTY 1 cut(s) 1004
XceI RCATGY 1 cut(s) 849
XmiI GTMKAC 1 cut(s) 852
XspI CTAG 4 cut(s) 41, 714, 722, 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.