Rh7AG392000

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0010630
Physical Location & Seq
Forward (+)
51304290 .. 51355010
50721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG392000.1

Sequence Viewer

Length: 699 bp
ATGGGTTGTCCAGATTTGTCGGATACCCATGATGATGGTGTCGCCGCTGCTGACCGTCGTCTTTTCGGAACCACAGAAGAGCCGCATGGGGATGAATCTAACGACGATGAGGATGACGCCGATAGGTTCAAACCTTACACACCGTCAAGCTCGCCGCAATTCCTTTACCGCAACCCGTCTTCTTCTTTCCGCTCCGTTTTCAAACCATTATCCAAAACCTTCCTCTACTGCCATAGCCACATACCAAGACCTCAAGTTCCTCTCTTCTTCGACGTAGCACCAAAAGCAACAAGCCCAGATGAGAAAGGTATGAAACCGCAGTGGAAAGCGATAGATTTCAAGTGGATAAGGGAGAACAAGGATGTTGTTGCTTGGTATTGCAGCCTAGTTTCATTGAATTTGGGTGATATCTGGTATGAGTCTGCTAGCAGGCTTGTTCATAACTCTTTGGTTACAGCTTCAAGATTATTATTCTCAAAGCAAACTATTCCTCATTATTATTTGACAGTAGATACATGTGTCGACAAGCTCGTTGAGTTGAGAGGCCAACTCAATTCATTGCAAGAAGCTACAGGTGGGAAGCGGATATCTATCAATGATCTTGTAATAAAGGCATCTTTACTCCAGAGTAATGCTTGGGAACTCCAGCGGGCTGGGCAATTTTCCTTCAGCTTGGAGTCAAACCTTTACAAGTTTTAA

Protein Analysis

232

Amino Acids

26.33

Weight (kDa)

5.87

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 151 - 208 1.8e-08 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 522
AciI CCGC 8 cut(s) 45, 83, 155, 169, 190, 317, 583, 649
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 652
AcyI GRCGYC 1 cut(s) 117
AflIII ACRYGT 1 cut(s) 515
AgsI TTSAA 5 cut(s) 130, 202, 340, 397, 462
AluBI AGCT 5 cut(s) 150, 458, 529, 569, 672
AluI AGCT 5 cut(s) 150, 458, 529, 569, 672
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 2 cut(s) 47, 381
ApoI RAATTY 1 cut(s) 397
AsuHPI GGTGA 1 cut(s) 416
AsuNHI GCTAGC 1 cut(s) 425
BaeI ACNNNNGTAYC 2 cut(s) 504, 537
BbsI GAAGAC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 34, 393
BccI CCATC 1 cut(s) 29
BciVI GTATCC 1 cut(s) 16
BfaI CTAG 2 cut(s) 386, 426
BfmI CTRYAG 1 cut(s) 570
BfuI GTATCC 1 cut(s) 16
BisI GCNGC 5 cut(s) 45, 48, 83, 155, 382
BlsI GCNGC 5 cut(s) 46, 49, 84, 156, 383
BmiI GGNNCC 1 cut(s) 70
BmsI GCATC 1 cut(s) 623
BmtI GCTAGC 1 cut(s) 429
BoxI GACNNNNGTC 1 cut(s) 57
BpiI GAAGAC 1 cut(s) 171
BplI GAGNNNNNCTC 2 cut(s) 534, 566
BpmI CTGGAG 2 cut(s) 608, 629
BpuEI CTTGAG 1 cut(s) 237
BsaBI GATNNNNATC 1 cut(s) 590
BsaHI GRCGYC 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 557
Bse8I GATNNNNATC 1 cut(s) 590
BseGI GGATG 3 cut(s) 97, 118, 367
BseJI GATNNNNATC 1 cut(s) 590
BseMI GCAATG 1 cut(s) 557
BseXI GCAGC 2 cut(s) 34, 393
BseYI CCCAGC 1 cut(s) 653
BshFI GGCC 1 cut(s) 546
BsnI GGCC 1 cut(s) 546
Bsp143I GATC 1 cut(s) 598
BspACI CCGC 8 cut(s) 45, 83, 155, 169, 190, 317, 583, 649
BspANI GGCC 1 cut(s) 546
BspLI GGNNCC 1 cut(s) 70
BspOI GCTAGC 1 cut(s) 429
BspQI GCTCTTC 1 cut(s) 72
BsrBI CCGCTC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 557
BssMI GATC 1 cut(s) 598
BssNI GRCGYC 1 cut(s) 117
Bst4CI ACNGT 3 cut(s) 56, 144, 508
Bst6I CTCTTC 2 cut(s) 72, 269
BstACI GRCGYC 1 cut(s) 117
BstC8I GCNNGC 4 cut(s) 152, 427, 431, 651
BstF5I GGATG 3 cut(s) 97, 118, 367
BstKTI GATC 1 cut(s) 601
BstMBI GATC 1 cut(s) 598
BstMWI GCNNNNNNNGC 2 cut(s) 284, 655
BstNSI RCATGY 1 cut(s) 519
BstPAI GACNNNNGTC 1 cut(s) 57
BstSFI CTRYAG 1 cut(s) 570
BstV1I GCAGC 2 cut(s) 34, 393
BstV2I GAAGAC 1 cut(s) 171
BstXI CCANNNNNNTGG 2 cut(s) 35, 653
BsuI GTATCC 1 cut(s) 16
BsuRI GGCC 1 cut(s) 546
BtsCI GGATG 3 cut(s) 97, 118, 367
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 1 cut(s) 326
Cac8I GCNNGC 4 cut(s) 152, 427, 431, 651
CseI GACGC 1 cut(s) 125
CviAII CATG 3 cut(s) 29, 86, 516
DpnI GATC 1 cut(s) 600
DpnII GATC 1 cut(s) 598
Eam1104I CTCTTC 2 cut(s) 72, 269
EarI CTCTTC 2 cut(s) 72, 269
Eco32I GATATC 2 cut(s) 409, 588
Eco57I CTGAAG 1 cut(s) 652
EcoRV GATATC 2 cut(s) 409, 588
FaeI CATG 3 cut(s) 32, 89, 519
FaiI YATR 8 cut(s) 30, 87, 234, 242, 311, 417, 441, 517
FatI CATG 3 cut(s) 28, 85, 515
FauI CCCGC 1 cut(s) 642
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 5 cut(s) 45, 48, 83, 155, 382
FokI GGATG 3 cut(s) 104, 125, 374
Fsp4HI GCNGC 5 cut(s) 45, 48, 83, 155, 382
FspBI CTAG 2 cut(s) 386, 426
GluI GCNGC 5 cut(s) 45, 48, 83, 155, 382
GsaI CCCAGC 1 cut(s) 657
GsuI CTGGAG 2 cut(s) 608, 629
HaeIII GGCC 1 cut(s) 546
HgaI GACGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 3 cut(s) 32, 89, 519
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 3 cut(s) 95, 419, 677
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 2 cut(s) 22, 68
Hpy188III TCNNGA 3 cut(s) 11, 462, 625
Hpy8I GTNNAC 1 cut(s) 523
Hpy99I CGWCG 3 cut(s) 60, 107, 275
HpyAV CCTTC 2 cut(s) 229, 676
HpyCH4III ACNGT 3 cut(s) 56, 144, 508
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 381, 562
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 655
HpySE526I ACGT 1 cut(s) 273
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 3 cut(s) 32, 89, 519
Kzo9I GATC 1 cut(s) 598
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 1 cut(s) 197
LpnPI CCDG 8 cut(s) 24, 309, 397, 415, 558, 638, 639, 659
Lsp1109I GCAGC 2 cut(s) 34, 393
LweI GCATC 1 cut(s) 623
MaeI CTAG 2 cut(s) 386, 426
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 1 cut(s) 451
MalI GATC 1 cut(s) 600
MbiI CCGCTC 1 cut(s) 192
MboI GATC 1 cut(s) 598
MboII GAAGA 5 cut(s) 89, 171, 174, 256, 259
MluCI AATT 4 cut(s) 158, 397, 553, 659
MlyI GAGTC 2 cut(s) 428, 686
MnlI CCTC 6 cut(s) 103, 233, 261, 270, 501, 536
MseI TTAA 1 cut(s) 697
MslI CAYNNNNRTG 2 cut(s) 33, 90
MspA1I CMGCKG 2 cut(s) 47, 649
MwoI GCNNNNNNNGC 2 cut(s) 284, 655
NdeII GATC 1 cut(s) 598
NheI GCTAGC 1 cut(s) 425
NlaIII CATG 3 cut(s) 32, 89, 519
NlaIV GGNNCC 1 cut(s) 70
NspI RCATGY 1 cut(s) 519
PciI ACATGT 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PfeI GAWTC 1 cut(s) 95
PkrI GCNGC 5 cut(s) 46, 49, 84, 156, 383
PleI GAGTC 2 cut(s) 427, 685
PpsI GAGTC 2 cut(s) 427, 685
PscI ACATGT 1 cut(s) 515
PshAI GACNNNNGTC 1 cut(s) 57
PspFI CCCAGC 1 cut(s) 653
PspN4I GGNNCC 1 cut(s) 70
RseI CAYNNNNRTG 2 cut(s) 33, 90
SalI GTCGAC 1 cut(s) 521
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 1 cut(s) 697
SatI GCNGC 5 cut(s) 45, 48, 83, 155, 382
Sau3AI GATC 1 cut(s) 598
SchI GAGTC 2 cut(s) 428, 686
SfaNI GCATC 1 cut(s) 623
SfcI CTRYAG 1 cut(s) 570
SmiMI CAYNNNNRTG 2 cut(s) 33, 90
SmlI CTYRAG 1 cut(s) 252
SmoI CTYRAG 1 cut(s) 252
Sse9I AATT 4 cut(s) 158, 397, 553, 659
SsiI CCGC 8 cut(s) 45, 83, 155, 169, 190, 317, 583, 649
SspMI CTAG 2 cut(s) 386, 426
TaaI ACNGT 3 cut(s) 56, 144, 508
TaiI ACGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 270, 522
TasI AATT 4 cut(s) 158, 397, 553, 659
TauI GCSGC 3 cut(s) 47, 85, 157
TfiI GAWTC 1 cut(s) 95
Tru1I TTAA 1 cut(s) 697
Tru9I TTAA 1 cut(s) 697
TscAI CASTG 1 cut(s) 326
TseI GCWGC 2 cut(s) 47, 381
TspDTI ATGAA 5 cut(s) 108, 326, 381, 428, 546
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 1 cut(s) 326
XapI RAATTY 1 cut(s) 397
XceI RCATGY 1 cut(s) 519
XmiI GTMKAC 1 cut(s) 522
XspI CTAG 2 cut(s) 386, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.