RLG00000034224

Cleavage site for pathogenic type III effector avirulence factor Avr

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008908
Physical Location & Seq
Forward (+)
38762420 .. 38764753
2334 bp
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UTR
Exon/CDS
Intron
RLM00000034224

Sequence Viewer

Length: 441 bp
ATGAACAATTATATTTGGTTGAAAGAACTGAAAATTGTTCAAGCAAGCTCGAGACTCCACAACTCCCAAAAGGTGGAAATCTTTCTATTCTGCAGCTCTCAAGTACAGTTTATCTCATTCTCTGATGTTGAGTACCTAGAATCCGCCGTTTTCCTGTGGGCAAGGGTTTGTTTGAGGATGAGAAAGCACACAAAGTTAATTCTTCAACATTGGCTTGAAGCTATTGATCCAGACCATTACCTTTCTTCTACTGACGGATACCCATCTGAATTGTGGGAAGGCACTACTGGAAATGCTATTATGTATTTATGTCCCCCTGCCATCATATGTTGCAAAAGACTTGGGACTATGTTCAAAAGCCGTGATGGGTCCTGCTGGAATCTGTTTGCTGTGTTTTTGAACATTTGGTGGAACTATGGGCTTATTTGGGAAGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AvrRpt-cleavage

Protein Analysis

147

Amino Acids

17.22

Weight (kDa)

6.88

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 73
AciI CCGC 1 cut(s) 144
AclWI GGATC 1 cut(s) 221
AfaI GTAC 2 cut(s) 105, 134
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 6 cut(s) 22, 41, 206, 218, 355, 400
AluBI AGCT 3 cut(s) 48, 96, 221
AluI AGCT 3 cut(s) 48, 96, 221
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 93
Asp700I GAANNNNTTC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 369
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 105
BccI CCATC 3 cut(s) 271, 329, 359
BceAI ACGGC 2 cut(s) 131, 345
BciVI GTATCC 1 cut(s) 251
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 91
BfuI GTATCC 1 cut(s) 251
BisI GCNGC 1 cut(s) 94
BlsI GCNGC 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 369
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 262
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 1 cut(s) 292
Bse8I GATNNNNATC 1 cut(s) 262
BseGI GGATG 1 cut(s) 183
BseJI GATNNNNATC 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 73
BseNI ACTGG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 49
BslFI GGGAC 2 cut(s) 297, 358
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 2 cut(s) 297, 358
BsoBI CYCGRG 1 cut(s) 49
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 370
BspMAI CTGCAG 1 cut(s) 95
BspPI GGATC 1 cut(s) 221
BsrI ACTGG 1 cut(s) 292
BssMI GATC 1 cut(s) 226
Bst4CI ACNGT 1 cut(s) 108
Bst6I CTCTTC 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 46
BstF5I GGATG 1 cut(s) 183
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 1 cut(s) 226
BstSFI CTRYAG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 105
BsuI GTATCC 1 cut(s) 251
BtsCI GGATG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 369
Csp6I GTAC 2 cut(s) 104, 133
CviJI RGCY 6 cut(s) 48, 96, 214, 221, 360, 421
CviKI_1 RGCY 6 cut(s) 48, 96, 214, 221, 360, 421
CviQI GTAC 2 cut(s) 104, 133
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 426
EarI CTCTTC 1 cut(s) 426
EciI GGCGGA 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 369
Eco88I CYCGRG 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 369
FaiI YATR 7 cut(s) 12, 302, 310, 326, 328, 350, 417
FaqI GGGAC 2 cut(s) 297, 358
FauNDI CATATG 1 cut(s) 326
Fnu4HI GCNGC 1 cut(s) 94
FokI GGATG 1 cut(s) 190
Fsp4HI GCNGC 1 cut(s) 94
FspBI CTAG 1 cut(s) 137
GluI GCNGC 1 cut(s) 94
HinfI GANTC 3 cut(s) 54, 140, 379
Hpy188I TCNGA 2 cut(s) 124, 268
Hpy188III TCNNGA 2 cut(s) 51, 230
HpyAV CCTTC 1 cut(s) 272
HpyCH4III ACNGT 1 cut(s) 108
HpyCH4V TGCA 2 cut(s) 93, 333
Kzo9I GATC 1 cut(s) 226
LpnPI CCDG 6 cut(s) 167, 243, 273, 330, 361, 385
Lsp1109I GCAGC 1 cut(s) 105
MaeI CTAG 1 cut(s) 137
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 2 cut(s) 194, 237
MluCI AATT 4 cut(s) 7, 33, 198, 269
MlyI GAGTC 1 cut(s) 48
MnlI CCTC 1 cut(s) 168
MroXI GAANNNNTTC 1 cut(s) 81
MseI TTAA 1 cut(s) 197
NdeI CATATG 1 cut(s) 326
NdeII GATC 1 cut(s) 226
NlaIV GGNNCC 1 cut(s) 370
PaeR7I CTCGAG 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 140, 379
PflMI CCANNNNNTGG 1 cut(s) 73
PkrI GCNGC 1 cut(s) 95
PleI GAGTC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 48
PpuMI RGGWCCY 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 369
PspN4I GGNNCC 1 cut(s) 370
PspPI GGNCC 1 cut(s) 369
PspPPI RGGWCCY 1 cut(s) 369
PstI CTGCAG 1 cut(s) 95
RsaI GTAC 2 cut(s) 105, 134
RsaNI GTAC 2 cut(s) 104, 133
SaqAI TTAA 1 cut(s) 197
SatI GCNGC 1 cut(s) 94
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 1 cut(s) 369
SchI GAGTC 1 cut(s) 48
SetI ASST 6 cut(s) 50, 75, 98, 138, 223, 243
SfcI CTRYAG 1 cut(s) 91
Sfr274I CTCGAG 1 cut(s) 49
SinI GGWCC 1 cut(s) 369
SlaI CTCGAG 1 cut(s) 49
SmlI CTYRAG 2 cut(s) 49, 99
SmoI CTYRAG 2 cut(s) 49, 99
Sse9I AATT 4 cut(s) 7, 33, 198, 269
SsiI CCGC 1 cut(s) 144
SspMI CTAG 1 cut(s) 137
TaaI ACNGT 1 cut(s) 108
TaqI TCGA 1 cut(s) 50
TasI AATT 4 cut(s) 7, 33, 198, 269
TatI WGTACW 1 cut(s) 103
TfiI GAWTC 2 cut(s) 140, 379
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
TseI GCWGC 1 cut(s) 93
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 270
Van91I CCANNNNNTGG 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 270
XhoI CTCGAG 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 81
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.