Rorug07G0242200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008908
Physical Location & Seq
Reverse (-)
22276285 .. 22277079
795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0242200.1

Sequence Viewer

Length: 534 bp
ATGGCTGACATCTCCGACGACGAATCCAAACCCCACCTCATCTTCTCCTACGGCACACTCAAGCGAGGCTTCCACAACCACAAGCTCACCAAGGAATTAATCGACCAAGACGACGCCGTTTTCATCGGCCCCTACGTTACCCTCCACACCTACCCCCTCGTCTGCGGCCCCCACGGATTGCCCTACCTCATCAACCTCCCCGGGTCGGGTCACCGGATCAGGGGCGAGCTCTACGCCGTTTCGGCCCGGGGACTCGCCCGGTTCGACGAGCTGGAAGGCACCTCCATCGGGCACTACGAGCGGCTCCCGGTTCAATTGATGGTCAATGCGGACAGAGACGACGTCGTTCCGGTCGAGGCCGAGGCCTATTTCGCGCACAGAAGCTTCGGGGAGGCCATGTGGGAGAGGAACGGGAAGGAGGGTTTGACCGAGTTCACGGAGAATGAGGCCAAATGGTACGTGAAAAGACAGGATAGGCCTCATCAGGGTCGCAATATTCTCGAGGACGTCCGGCATTTTGTGAATGGAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

20.15

Weight (kDa)

5.78

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 14 - 127 2e-18 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 345, 510
AccB1I GGYRCC 1 cut(s) 278
AccBSI CCGCTC 1 cut(s) 301
AccII CGCG 1 cut(s) 374
AciI CCGC 3 cut(s) 165, 301, 329
AclWI GGATC 1 cut(s) 224
AcyI GRCGYC 3 cut(s) 114, 342, 507
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
AgsI TTSAA 1 cut(s) 314
AluBI AGCT 4 cut(s) 85, 229, 271, 384
AluI AGCT 4 cut(s) 85, 229, 271, 384
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 330
AlwI GGATC 1 cut(s) 224
Ama87I CYCGRG 3 cut(s) 200, 246, 500
AoxI GGCC 8 cut(s) 127, 166, 243, 357, 363, 393, 447, 476
AseI ATTAAT 1 cut(s) 98
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 3 cut(s) 128, 167, 244
AsuC2I CCSGG 6 cut(s) 201, 202, 247, 248, 259, 308
AsuHPI GGTGA 2 cut(s) 79, 203
AvaI CYCGRG 3 cut(s) 200, 246, 500
BaeGI GKGCMC 1 cut(s) 294
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 2 cut(s) 293, 313
BceAI ACGGC 3 cut(s) 67, 101, 221
BcgI CGANNNNNNTGC 4 cut(s) 268, 302, 481, 515
BcnI CCSGG 6 cut(s) 201, 202, 247, 248, 259, 308
BcoDI GTCTC 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 242
BisI GCNGC 2 cut(s) 166, 302
BlsI GCNGC 2 cut(s) 167, 303
Bme1390I CCNGG 6 cut(s) 201, 202, 247, 248, 259, 308
BmeT110I CYCGRG 3 cut(s) 200, 246, 500
BmgT120I GGNCC 3 cut(s) 128, 167, 244
BmiI GGNNCC 4 cut(s) 130, 169, 280, 305
BmrFI CCNGG 6 cut(s) 201, 202, 247, 248, 259, 308
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 6 cut(s) 201, 202, 247, 248, 259, 308
BsaAI YACGTR 1 cut(s) 460
BsaHI GRCGYC 3 cut(s) 114, 342, 507
BsaJI CCNNGG 7 cut(s) 90, 172, 199, 200, 246, 247, 360
BsaWI WCCGGW 2 cut(s) 213, 349
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
BseDI CCNNGG 7 cut(s) 90, 172, 199, 200, 246, 247, 360
BseLI CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
BseSI GKGCMC 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 374
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 8 cut(s) 129, 168, 245, 359, 365, 395, 449, 478
BshNI GGYRCC 1 cut(s) 278
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 3 cut(s) 200, 246, 500
BsiSI CCGG 7 cut(s) 201, 214, 247, 259, 308, 350, 511
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
BsmAI GTCTC 1 cut(s) 330
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 8 cut(s) 129, 168, 245, 359, 365, 395, 449, 478
BsoBI CYCGRG 3 cut(s) 200, 246, 500
Bsp1286I GDGCHC 2 cut(s) 231, 294
Bsp143I GATC 1 cut(s) 216
BspACI CCGC 3 cut(s) 165, 301, 329
BspANI GGCC 8 cut(s) 129, 168, 245, 359, 365, 395, 449, 478
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 4 cut(s) 130, 169, 280, 305
BspPI GGATC 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 278
BsrBI CCGCTC 1 cut(s) 301
BssECI CCNNGG 7 cut(s) 90, 172, 199, 200, 246, 247, 360
BssMI GATC 1 cut(s) 216
BssNI GRCGYC 3 cut(s) 114, 342, 507
BssT1I CCWWGG 1 cut(s) 90
BstACI GRCGYC 3 cut(s) 114, 342, 507
BstBAI YACGTR 1 cut(s) 460
BstC8I GCNNGC 1 cut(s) 227
BstDSI CCRYGG 1 cut(s) 172
BstEII GGTNACC 1 cut(s) 209
BstFNI CGCG 1 cut(s) 374
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 1 cut(s) 216
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 3 cut(s) 242, 298, 371
BstPI GGTNACC 1 cut(s) 209
BstSCI CCNGG 6 cut(s) 199, 200, 245, 246, 257, 306
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 1 cut(s) 374
BsuRI GGCC 8 cut(s) 129, 168, 245, 359, 365, 395, 449, 478
BtgI CCRYGG 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 227
CfoI GCGC 1 cut(s) 376
Cfr13I GGNCC 3 cut(s) 128, 167, 244
Cfr9I CCCGGG 2 cut(s) 200, 246
CseI GACGC 1 cut(s) 122
Csp6I GTAC 1 cut(s) 457
CviAII CATG 1 cut(s) 397
CviQI GTAC 1 cut(s) 457
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 365, 478
Eco24I GRGCYC 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 229
Eco88I CYCGRG 3 cut(s) 200, 246, 500
Eco91I GGTNACC 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 1 cut(s) 400
FaiI YATR 1 cut(s) 398
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 264
FatI CATG 1 cut(s) 396
Fnu4HI GCNGC 2 cut(s) 166, 302
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 2 cut(s) 166, 302
GlaI GCGC 1 cut(s) 375
GluI GCNGC 2 cut(s) 166, 302
HaeIII GGCC 8 cut(s) 129, 168, 245, 359, 365, 395, 449, 478
HapII CCGG 7 cut(s) 201, 214, 247, 259, 308, 350, 511
HgaI GACGC 1 cut(s) 122
HhaI GCGC 1 cut(s) 376
Hin1I GRCGYC 3 cut(s) 114, 342, 507
Hin1II CATG 1 cut(s) 400
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HindIII AAGCTT 1 cut(s) 382
HinfI GANTC 2 cut(s) 23, 252
HpaII CCGG 7 cut(s) 201, 214, 247, 259, 308, 350, 511
HphI GGTGA 2 cut(s) 79, 203
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 1 cut(s) 435
Hpy99I CGWCG 6 cut(s) 20, 23, 116, 269, 344, 347
HpyAV CCTTC 2 cut(s) 269, 409
HpyCH4IV ACGT 4 cut(s) 135, 342, 459, 507
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 298, 371
HpySE526I ACGT 4 cut(s) 135, 342, 459, 507
Hsp92I GRCGYC 3 cut(s) 114, 342, 507
Hsp92II CATG 1 cut(s) 400
HspAI GCGC 1 cut(s) 374
Kzo9I GATC 1 cut(s) 216
LmnI GCTCC 1 cut(s) 309
MaeII ACGT 4 cut(s) 135, 342, 459, 507
MaeIII GTNAC 2 cut(s) 136, 209
MalI GATC 1 cut(s) 218
MbiI CCGCTC 1 cut(s) 301
MboI GATC 1 cut(s) 216
MboII GAAGA 1 cut(s) 34
MfeI CAATTG 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 231, 294
MluCI AATT 2 cut(s) 95, 314
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 39
MseI TTAA 1 cut(s) 98
MspI CCGG 7 cut(s) 201, 214, 247, 259, 308, 350, 511
MspR9I CCNGG 6 cut(s) 201, 202, 247, 248, 259, 308
MunI CAATTG 1 cut(s) 314
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 3 cut(s) 242, 298, 371
NciI CCSGG 6 cut(s) 201, 202, 247, 248, 259, 308
NdeII GATC 1 cut(s) 216
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 4 cut(s) 130, 169, 280, 305
NmeAIII GCCGAG 1 cut(s) 385
NmuCI GTSAC 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 500
PceI AGGCCT 2 cut(s) 365, 478
PcsI WCGNNNNNNNCGW 5 cut(s) 108, 132, 261, 294, 351
PfeI GAWTC 1 cut(s) 23
PflFI GACNNNGTC 1 cut(s) 341
PkrI GCNGC 2 cut(s) 167, 303
PleI GAGTC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 246
Ppu21I YACGTR 1 cut(s) 460
PshBI ATTAAT 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 231
PspEI GGTNACC 1 cut(s) 209
PspN4I GGNNCC 4 cut(s) 130, 169, 280, 305
PspPI GGNCC 3 cut(s) 128, 167, 244
PsyI GACNNNGTC 1 cut(s) 341
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 2 cut(s) 166, 302
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 3 cut(s) 128, 167, 244
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 6 cut(s) 201, 202, 247, 248, 259, 308
SduI GDGCHC 2 cut(s) 231, 294
Sfr274I CTCGAG 1 cut(s) 500
SlaI CTCGAG 1 cut(s) 500
SmaI CCCGGG 2 cut(s) 202, 248
SmlI CTYRAG 2 cut(s) 59, 500
SmoI CTYRAG 2 cut(s) 59, 500
Sse9I AATT 2 cut(s) 95, 314
SseBI AGGCCT 2 cut(s) 365, 478
SsiI CCGC 3 cut(s) 165, 301, 329
SspI AATATT 1 cut(s) 496
SstI GAGCTC 1 cut(s) 231
StuI AGGCCT 2 cut(s) 365, 478
StyD4I CCNGG 6 cut(s) 199, 200, 245, 246, 257, 306
StyI CCWWGG 1 cut(s) 90
TaiI ACGT 4 cut(s) 138, 345, 462, 510
TaqI TCGA 4 cut(s) 102, 264, 354, 501
TaqII GACCGA 1 cut(s) 443
TasI AATT 2 cut(s) 95, 314
TauI GCSGC 2 cut(s) 168, 304
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TseFI GTSAC 1 cut(s) 209
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 112
TspGWI ACGGA 2 cut(s) 189, 452
TspMI CCCGGG 2 cut(s) 200, 246
Tth111I GACNNNGTC 1 cut(s) 341
VspI ATTAAT 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 500
XmaI CCCGGG 2 cut(s) 200, 246
ZraI GACGTC 2 cut(s) 343, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.