Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0008908

Physical Location & Seq

Genomic Coordinates Forward (+)

3895997 .. 3899274

Gene Structure 3278 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0044200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGCCACTGTTTCATACCAACAAAGTTGCAAATGCCCAGAATATGTTATTCAAAGCTCTGCTTCTCTTCCTCCTCTTCCGATTTTGCGAATCCAGGGACACCATAACATGGAACCAAGAACAACTGAAGGATGATGGTGGAGTTTTGGTGTCCAAACAGAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTGGGAATTTGGTATTCTCAGACTAGAGTATCTAATAAAATAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATAAACAGGTATGGAGAACTAGTCCTTTATGCTTATAACATGGAGAGCAGTCCTACTTGGTCTACTAATGTGTCGCGGTCGGTTCAAAATGTTAACACAAGCACTTTATCTGCACAGCTTTTAGATACAGGAAATCTAGTTGTGTTCCAGGATGATAATAATGAAAGCTTTTTATGGCAAAGTTTTGATCATCCCACAGATACTTTAATTCCAGGTATGAAAGTTGGGATGAATTGGAAAACTGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTTAAGGCTCTATCCAAATCAGATTGCATCCGCCCAATTCATTATGTACAAGGGTTCGAGTAAGTATTGGCGAAGTGATCCAGGGATAACGGGTTTGGTCACTAATCAAGAGGAAACTTATTATCCCATGAATAACACCAATGCAATTACAAGAGCAACAGTGATTGATTCTGCGGTAGAAGGCTTTACATGGGATGATGGTGGCCTTCAATGGAAGGTAGGCTTCTCTGCACCGAAGTCCCGGTGTGACTGGTATGGAGAGTGCGGTGCCAACAGCAAATGTCTCCCTGACAATGTTATTATGTTTTGGTGTGATTGTTTACCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCATAAAAATGGGTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTGTGAAGGTGGCAAGAGTTAAATATCCTGACACATCGATAGGAGCATTGTCAAAATCAGGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTACTTGCACTGCATATTTGAGCACTGAAAATGAAGGGCGTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTAAAATGTACACAGAGATTGGACAAGATCTCTACGTTCGTGTGAATGCGACCGTGTTAGCTGCAAATGCCAGAAGATCAGAAGGTTTCTTGGAAAGGAGGGGTATGCTGGCTATTCCAATACTGTCTGTTGTGCTAGCATTGGTACTAATCATTATGCTTGCATGTTGGTGGCGCAAGAAGAACAGGAACACAAAAGACTTTGTGGAGGCAGAGGAGCTTGTGGAAAAACAGAGACACCCTGAATTGCAATTTTTCGATCTTGACACAATAGTCGCAGCAACAGACCACTTCTCTCGTGTCAATGAACTTGGCCATGGTGGATTTGGCTCTGTTTATAAGGGTCAGCTACCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCACAAACTTCAGGACAAGGGATCGAAGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTGCTAGAATACATGCCTAACAAAAGCTTGAACTCCTTTCTTTTTGATAACACAAGACGGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATTATCAACGGGATTGCTCGTGGGATTCTGTATCTTCACCAAGACTCAAGATTGAGGATCATCCATAGAGATCTGAAAACTAGCAATATTCTTCTAGATGCTGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCACGGAGATCAACTGCAAGATAAGACAAGCCGAATTGTTGGCACATATGGCTACATGTCACCAGAGTACGCAGTATTTGGAAGATTTTCCATGAAATCTGATGTATTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCGGAAAAACAATGATTCTGATCTGGAGGATCCTTCCATGAACTTGATAGCACGTGTTTGGGAGCTCTGGGGTGAAGGCAGAGCCTTAGATATTGTTGATTCGACACTGAAGTCATATCAGCCTAATGAAGTAATGAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCAGCCATCGTTTTCATGTTGAGTGGTGAAGCATCTCCCCCATTGCCTAAGCATCCAGCATTTGTTTACAGAAGAGACTCTGGCGTTGATGTTGATCCATTACTTCCAAATGGATCTTATTCTATAAATGACTTGACAATAACTACAATGGAAGCTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

821

Amino Acids

92.8

Weight (kDa)

5.83

Isoelectric Point (pI)

39.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	85 - 194	1.2e-35	D-mannose binding lectin
S_locus_glycop	PF00954	239 - 318	9.7e-12	S-locus glycoprotein domain
PAN_2	PF08276	342 - 408	3.7e-17	PAN-like domain
Pkinase	PF00069	506 - 771	2.8e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	508 - 773	5.3e-51	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008908)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr4g0403241 RchiOBHm_Chr5g0028651
rosa_laevigata	RLG00000013260 RLG00000034224
rosa_roxburghii	Rroxscaffold_1G00002440 Rroxscaffold_3G00225880 Rroxscaffold_4G00299590 Rroxscaffold_7G00185090
rosa_rugosa	Rorug03G0240800 Rorug04G0233800 Rorug05G0020300 Rorug05G0044200 Rorug05G0479100 Rorug06G0053500 Rorug07G0242200
rosa_samantha	Rh2AG235100 Rh2BG257000 Rh4BG112100 Rh4CG280300 Rh5DG233000 Rh6AG271200 Rh6CG262100
rosa_wichuraiana	Rw2G021950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	345, 1560
AasI	GACNNNNNNGTC	2 cut(s)	1493, 2218
Acc36I	ACCTGC	1 cut(s)	2305
AccB1I	GGYRCC	1 cut(s)	881
AccB7I	CCANNNNNTGG	1 cut(s)	108
AccI	GTMKAC	1 cut(s)	371
AccII	CGCG	1 cut(s)	385
AciI	CCGC	4 cut(s)	385, 645, 788, 879
AclWI	GGATC	8 cut(s)	686, 1634, 1883, 2090, 2132, 2145, 2393, 2425
AcoI	YGGCCR	2 cut(s)	1534, 2107
AcsI	RAATTY	4 cut(s)	193, 216, 1634, 1935
AcuI	CTGAAG	4 cut(s)	146, 166, 1599, 2237
AcvI	CACGTG	1 cut(s)	2162
AfaI	GTAC	6 cut(s)	662, 1147, 1232, 1368, 2033, 2274
AfeI	AGCGCT	1 cut(s)	1811
AfiI	CCNNNNNNNGG	3 cut(s)	108, 211, 602
AflII	CTTAAG	1 cut(s)	616
AflIII	ACRYGT	2 cut(s)	2019, 2161
AgeI	ACCGGT	1 cut(s)	204
AhlI	ACTAGT	1 cut(s)	328
AjnI	CCWGG	6 cut(s)	92, 456, 520, 595, 694, 937
Alw21I	GWGCWC	3 cut(s)	306, 1169, 2175
Alw26I	GTCTC	4 cut(s)	902, 1029, 1450, 2373
AlwI	GGATC	8 cut(s)	686, 1634, 1883, 2090, 2132, 2145, 2393, 2425
Aor51HI	AGCGCT	1 cut(s)	1811
AoxI	GGCC	3 cut(s)	817, 1534, 2107
ApeKI	GCWGC	2 cut(s)	1283, 1499
ApoI	RAATTY	4 cut(s)	193, 216, 1634, 1935
AsiGI	ACCGGT	1 cut(s)	204
Asp700I	GAANNNNTTC	1 cut(s)	2050
AspLEI	GCGC	3 cut(s)	1398, 1812, 2263
AspS9I	GGNCC	2 cut(s)	1788, 2290
AsuC2I	CCSGG	2 cut(s)	189, 856
AsuHPI	GGTGA	4 cut(s)	1847, 2016, 2192, 2342
AsuNHI	GCTAGC	1 cut(s)	1357
AvaII	GGWCC	2 cut(s)	1788, 2290
BaeI	ACNNNNGTAYC	2 cut(s)	222, 255
BalI	TGGCCA	1 cut(s)	1536
BamHI	GGATCC	1 cut(s)	2137
BanI	GGYRCC	1 cut(s)	881
BanII	GRGCYC	1 cut(s)	2175
BauI	CACGAG	2 cut(s)	1518, 1836
BbrPI	CACGTG	1 cut(s)	2162
Bbv12I	GWGCWC	3 cut(s)	306, 1169, 2175
BbvI	GCAGC	2 cut(s)	1270, 1511
BccI	CCATC	6 cut(s)	128, 290, 806, 1025, 1210, 2318
BciT130I	CCWGG	6 cut(s)	94, 458, 522, 597, 696, 939
BclI	TGATCA	1 cut(s)	496
BcnI	CCSGG	2 cut(s)	189, 856
BcoDI	GTCTC	4 cut(s)	902, 1029, 1450, 2373
BcuI	ACTAGT	1 cut(s)	328
BfaI	CTAG	9 cut(s)	198, 234, 329, 446, 1358, 1733, 1899, 1913, 1956
BfoI	RGCGCY	1 cut(s)	1813
BfrI	CTTAAG	1 cut(s)	616
BfuAI	ACCTGC	1 cut(s)	2305
BglII	AGATCT	2 cut(s)	1249, 1888
BisI	GCNGC	2 cut(s)	1284, 1500
BlsI	GCNGC	2 cut(s)	1285, 1501
Bme1390I	CCNGG	8 cut(s)	94, 189, 458, 522, 597, 696, 856, 939
Bme18I	GGWCC	2 cut(s)	1788, 2290
BmgT120I	GGNCC	2 cut(s)	1788, 2290
BmiI	GGNNCC	3 cut(s)	113, 883, 2139
BmrFI	CCNGG	8 cut(s)	94, 189, 458, 522, 597, 696, 856, 939
BmrI	ACTGGG	2 cut(s)	561, 612
BmsI	GCATC	5 cut(s)	650, 1906, 2237, 2345, 2365
BmtI	GCTAGC	1 cut(s)	1361
BmuI	ACTGGG	2 cut(s)	561, 612
BpmI	CTGGAG	1 cut(s)	2153
Bpu10I	CCTNAGC	1 cut(s)	2352
BpuEI	CTTGAG	1 cut(s)	1849
BpuMI	CCSGG	2 cut(s)	189, 856
Bsa29I	ATCGAT	1 cut(s)	1079
BsaAI	YACGTR	1 cut(s)	2162
BsaBI	GATNNNNATC	3 cut(s)	969, 2127, 2397
BsaJI	CCNNGG	7 cut(s)	93, 187, 595, 695, 938, 1537, 1966
BsaWI	WCCGGW	1 cut(s)	204
BsaXI	ACNNNNNCTCC	4 cut(s)	132, 162, 315, 345
Bsc4I	CCNNNNNNNGG	3 cut(s)	108, 211, 602
Bse118I	RCCGGY	1 cut(s)	204
Bse1I	ACTGG	3 cut(s)	556, 607, 869
Bse3DI	GCAATG	1 cut(s)	2345
Bse8I	GATNNNNATC	3 cut(s)	969, 2127, 2397
BseBI	CCWGG	6 cut(s)	94, 458, 522, 597, 696, 939
BseCI	ATCGAT	1 cut(s)	1079
BseDI	CCNNGG	7 cut(s)	93, 187, 595, 695, 938, 1537, 1966
BseJI	GATNNNNATC	3 cut(s)	969, 2127, 2397
BseLI	CCNNNNNNNGG	3 cut(s)	108, 211, 602
BseMI	GCAATG	1 cut(s)	2345
BseMII	CTCAG	2 cut(s)	242, 1911
BseNI	ACTGG	3 cut(s)	556, 607, 869
BseRI	GAGGAG	2 cut(s)	62, 1451
BseXI	GCAGC	2 cut(s)	1270, 1511
BsgI	GTGCAG	2 cut(s)	405, 828
Bsh1236I	CGCG	1 cut(s)	385
Bsh1285I	CGRYCG	2 cut(s)	389, 1275
BshFI	GGCC	3 cut(s)	819, 1536, 2109
BshNI	GGYRCC	1 cut(s)	881
BshTI	ACCGGT	1 cut(s)	204
BshVI	ATCGAT	1 cut(s)	1079
BsiEI	CGRYCG	2 cut(s)	389, 1275
BsiHKAI	GWGCWC	3 cut(s)	306, 1169, 2175
BsiSI	CCGG	4 cut(s)	189, 205, 856, 2110
BslFI	GGGAC	3 cut(s)	110, 620, 838
BslI	CCNNNNNNNGG	3 cut(s)	108, 211, 602
BsmAI	GTCTC	4 cut(s)	902, 1029, 1450, 2373
BsmBI	CGTCTC	1 cut(s)	1029
BsmFI	GGGAC	3 cut(s)	110, 620, 838
BsmI	GAATGC	1 cut(s)	1273
BsnI	GGCC	3 cut(s)	819, 1536, 2109
Bsp1286I	GDGCHC	3 cut(s)	306, 1169, 2175
Bsp1407I	TGTACA	3 cut(s)	660, 1230, 2272
Bsp19I	CCATGG	1 cut(s)	1537
BspACI	CCGC	4 cut(s)	385, 645, 788, 879
BspANI	GGCC	3 cut(s)	819, 1536, 2109
BspCNI	CTCAG	2 cut(s)	241, 1912
BspDI	ATCGAT	1 cut(s)	1079
BspFNI	CGCG	1 cut(s)	385
BspLI	GGNNCC	3 cut(s)	113, 883, 2139
BspMI	ACCTGC	1 cut(s)	2305
BspOI	GCTAGC	1 cut(s)	1361
BspPI	GGATC	8 cut(s)	686, 1634, 1883, 2090, 2132, 2145, 2393, 2425
BspT107I	GGYRCC	1 cut(s)	881
BspTI	CTTAAG	1 cut(s)	616
BsrDI	GCAATG	1 cut(s)	2345
BsrFI	RCCGGY	1 cut(s)	204
BsrGI	TGTACA	3 cut(s)	660, 1230, 2272
BsrI	ACTGG	3 cut(s)	556, 607, 869
BssAI	RCCGGY	1 cut(s)	204
BssECI	CCNNGG	7 cut(s)	93, 187, 595, 695, 938, 1537, 1966
BssSI	CACGAG	2 cut(s)	1518, 1836
BssT1I	CCWWGG	1 cut(s)	1537
Bst2BI	CACGAG	2 cut(s)	1518, 1836
Bst2UI	CCWGG	6 cut(s)	94, 458, 522, 597, 696, 939
Bst4CI	ACNGT	5 cut(s)	9, 775, 1276, 1347, 1788
Bst6I	CTCTTC	3 cut(s)	71, 80, 2371
BstAFI	CTTAAG	1 cut(s)	616
BstAUI	TGTACA	3 cut(s)	660, 1230, 2272
BstBAI	YACGTR	1 cut(s)	2162
BstC8I	GCNNGC	3 cut(s)	1332, 1359, 1383
BstDEI	CTNAG	5 cut(s)	228, 1136, 1920, 2194, 2352
BstDSI	CCRYGG	2 cut(s)	1537, 1966
BstFNI	CGCG	1 cut(s)	385
BstH2I	RGCGCY	1 cut(s)	1813
BstHHI	GCGC	3 cut(s)	1398, 1812, 2263
BstMAI	GTCTC	4 cut(s)	902, 1029, 1450, 2373
BstMCI	CGRYCG	2 cut(s)	389, 1275
BstMWI	GCNNNNNNNGC	4 cut(s)	1289, 1658, 2013, 2306
BstNI	CCWGG	6 cut(s)	94, 458, 522, 597, 696, 939
BstNSI	RCATGY	3 cut(s)	1389, 1744, 2023
BstSCI	CCNGG	8 cut(s)	92, 187, 456, 520, 595, 694, 854, 937
BstUI	CGCG	1 cut(s)	385
BstV1I	GCAGC	2 cut(s)	1270, 1511
BstX2I	RGATCY	4 cut(s)	1249, 1888, 2137, 2417
BstYI	RGATCY	4 cut(s)	1249, 1888, 2137, 2417
Bsu15I	ATCGAT	1 cut(s)	1079
BsuRI	GGCC	3 cut(s)	819, 1536, 2109
BsuTUI	ATCGAT	1 cut(s)	1079
BtgI	CCRYGG	2 cut(s)	1537, 1966
BtsI	GCAGTG	1 cut(s)	1152
BtsIMutI	CAGTG	5 cut(s)	5, 780, 1152, 1167, 2213
BveI	ACCTGC	1 cut(s)	2305
Cac8I	GCNNGC	3 cut(s)	1332, 1359, 1383
CfoI	GCGC	3 cut(s)	1398, 1812, 2263
Cfr10I	RCCGGY	1 cut(s)	204
Cfr13I	GGNCC	2 cut(s)	1788, 2290
ClaI	ATCGAT	1 cut(s)	1079
Csp6I	GTAC	6 cut(s)	661, 1146, 1231, 1367, 2032, 2273
CspAI	ACCGGT	1 cut(s)	204
CviQI	GTAC	6 cut(s)	661, 1146, 1231, 1367, 2032, 2273
DdeI	CTNAG	5 cut(s)	228, 1136, 1920, 2194, 2352
DrdI	GACNNNNNNGTC	2 cut(s)	1493, 2218
DseDI	GACNNNNNNGTC	2 cut(s)	1493, 2218
EaeI	YGGCCR	2 cut(s)	1534, 2107
Eam1104I	CTCTTC	3 cut(s)	71, 80, 2371
EarI	CTCTTC	3 cut(s)	71, 80, 2371
EciI	GGCGGA	1 cut(s)	634
Ecl136II	GAGCTC	1 cut(s)	2173
Eco130I	CCWWGG	1 cut(s)	1537
Eco24I	GRGCYC	1 cut(s)	2175
Eco47I	GGWCC	2 cut(s)	1788, 2290
Eco47III	AGCGCT	1 cut(s)	1811
Eco53kI	GAGCTC	1 cut(s)	2173
Eco57I	CTGAAG	4 cut(s)	146, 166, 1599, 2237
Eco72I	CACGTG	1 cut(s)	2162
EcoICRI	GAGCTC	1 cut(s)	2173
EcoRI	GAATTC	1 cut(s)	1634
EcoRII	CCWGG	6 cut(s)	92, 456, 520, 595, 694, 937
EcoT14I	CCWWGG	1 cut(s)	1537
EcoT22I	ATGCAT	1 cut(s)	2252
EcoT38I	GRGCYC	1 cut(s)	2175
ErhI	CCWWGG	1 cut(s)	1537
Esp3I	CGTCTC	1 cut(s)	1029
FalI	AAGNNNNNCTT	6 cut(s)	45, 77, 821, 853, 1638, 1670
FaqI	GGGAC	3 cut(s)	110, 620, 838
FauNDI	CATATG	1 cut(s)	2011
FbaI	TGATCA	1 cut(s)	496
FblI	GTMKAC	1 cut(s)	371
Fnu4HI	GCNGC	2 cut(s)	1284, 1500
FriOI	GRGCYC	1 cut(s)	2175
Fsp4HI	GCNGC	2 cut(s)	1284, 1500
FspBI	CTAG	9 cut(s)	198, 234, 329, 446, 1358, 1733, 1899, 1913, 1956
GlaI	GCGC	3 cut(s)	1397, 1811, 2262
GluI	GCNGC	2 cut(s)	1284, 1500
GsuI	CTGGAG	1 cut(s)	2153
HaeII	RGCGCY	1 cut(s)	1813
HaeIII	GGCC	3 cut(s)	819, 1536, 2109
HapII	CCGG	4 cut(s)	189, 205, 856, 2110
HhaI	GCGC	3 cut(s)	1398, 1812, 2263
Hin6I	GCGC	3 cut(s)	1396, 1810, 2261
HinP1I	GCGC	3 cut(s)	1396, 1810, 2261
HincII	GTYRAC	1 cut(s)	403
HindII	GTYRAC	1 cut(s)	403
HindIII	AAGCTT	2 cut(s)	475, 1753
HpaI	GTTAAC	1 cut(s)	403
HpaII	CCGG	4 cut(s)	189, 205, 856, 2110
HphI	GGTGA	4 cut(s)	1847, 2016, 2192, 2342
Hpy166II	GTNNAC	6 cut(s)	372, 403, 935, 1233, 2273, 2371
Hpy188I	TCNGA	8 cut(s)	80, 231, 636, 1303, 1893, 1942, 2065, 2128
Hpy188III	TCNNGA	7 cut(s)	722, 1070, 1484, 1617, 1866, 1913, 2132
Hpy8I	GTNNAC	6 cut(s)	372, 403, 935, 1233, 2273, 2371
HpyCH4III	ACNGT	5 cut(s)	9, 775, 1276, 1347, 1788
HpyCH4IV	ACGT	2 cut(s)	1257, 2161
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1289, 1658, 2013, 2306
HpyF3I	CTNAG	5 cut(s)	228, 1136, 1920, 2194, 2352
HpySE526I	ACGT	2 cut(s)	1257, 2161
HspAI	GCGC	3 cut(s)	1396, 1810, 2261
Ksp22I	TGATCA	1 cut(s)	496
KspAI	GTTAAC	1 cut(s)	403
LmnI	GCTCC	3 cut(s)	1085, 1438, 2170
Lsp1109I	GCAGC	2 cut(s)	1270, 1511
LweI	GCATC	5 cut(s)	650, 1906, 2237, 2345, 2365
MaeI	CTAG	9 cut(s)	198, 234, 329, 446, 1358, 1733, 1899, 1913, 1956
MaeII	ACGT	2 cut(s)	1257, 2161
MaeIII	GTNAC	5 cut(s)	582, 712, 860, 1605, 2022
MflI	RGATCY	4 cut(s)	1249, 1888, 2137, 2417
MhlI	GDGCHC	3 cut(s)	306, 1169, 2175
MlsI	TGGCCA	1 cut(s)	1536
MluNI	TGGCCA	1 cut(s)	1536
MlyI	GAGTC	2 cut(s)	1856, 2375
MmeI	TCCRAC	1 cut(s)	2073
MnlI	CCTC	9 cut(s)	80, 83, 304, 718, 1314, 1423, 1429, 1866, 2128
Mox20I	TGGCCA	1 cut(s)	1536
Mph1103I	ATGCAT	1 cut(s)	2252
MroXI	GAANNNNTTC	1 cut(s)	2050
MscI	TGGCCA	1 cut(s)	1536
MseI	TTAA	6 cut(s)	402, 515, 569, 617, 1062, 1224
MslI	CAYNNNNRTG	3 cut(s)	978, 1226, 1390
Msp20I	TGGCCA	1 cut(s)	1536
MspCI	CTTAAG	1 cut(s)	616
MspI	CCGG	4 cut(s)	189, 205, 856, 2110
MspR9I	CCNGG	8 cut(s)	94, 189, 458, 522, 597, 696, 856, 939
Mva1269I	GAATGC	1 cut(s)	1273
MvaI	CCWGG	6 cut(s)	94, 458, 522, 597, 696, 939
MvnI	CGCG	1 cut(s)	385
MwoI	GCNNNNNNNGC	4 cut(s)	1289, 1658, 2013, 2306
NciI	CCSGG	2 cut(s)	189, 856
NcoI	CCATGG	1 cut(s)	1537
NdeI	CATATG	1 cut(s)	2011
NheI	GCTAGC	1 cut(s)	1357
NlaIV	GGNNCC	3 cut(s)	113, 883, 2139
NmuCI	GTSAC	5 cut(s)	582, 712, 860, 1605, 2022
NsiI	ATGCAT	1 cut(s)	2252
NspI	RCATGY	3 cut(s)	1389, 1744, 2023
PciI	ACATGT	1 cut(s)	2019
PctI	GAATGC	1 cut(s)	1273
PdmI	GAANNNNTTC	1 cut(s)	2050
PfeI	GAWTC	8 cut(s)	89, 782, 970, 1678, 1843, 2123, 2207, 2282
PflMI	CCANNNNNTGG	1 cut(s)	108
PfoI	TCCNGGA	1 cut(s)	456
PinAI	ACCGGT	1 cut(s)	204
PkrI	GCNGC	2 cut(s)	1285, 1501
PleI	GAGTC	2 cut(s)	1856, 2375
PmaCI	CACGTG	1 cut(s)	2162
PmlI	CACGTG	1 cut(s)	2162
PpsI	GAGTC	2 cut(s)	1856, 2375
Ppu21I	YACGTR	1 cut(s)	2162
PscI	ACATGT	1 cut(s)	2019
PsiI	TTATAA	2 cut(s)	345, 1560
Psp124BI	GAGCTC	1 cut(s)	2175
Psp6I	CCWGG	6 cut(s)	92, 456, 520, 595, 694, 937
PspCI	CACGTG	1 cut(s)	2162
PspGI	CCWGG	6 cut(s)	92, 456, 520, 595, 694, 937
PspN4I	GGNNCC	3 cut(s)	113, 883, 2139
PspPI	GGNCC	2 cut(s)	1788, 2290
PsuI	RGATCY	4 cut(s)	1249, 1888, 2137, 2417
RsaI	GTAC	6 cut(s)	662, 1147, 1232, 1368, 2033, 2274
RsaNI	GTAC	6 cut(s)	661, 1146, 1231, 1367, 2032, 2273
RseI	CAYNNNNRTG	3 cut(s)	978, 1226, 1390
SacI	GAGCTC	1 cut(s)	2175
SaqAI	TTAA	6 cut(s)	402, 515, 569, 617, 1062, 1224
SatI	GCNGC	2 cut(s)	1284, 1500
Sau96I	GGNCC	2 cut(s)	1788, 2290
SchI	GAGTC	2 cut(s)	1856, 2375
ScrFI	CCNGG	8 cut(s)	94, 189, 458, 522, 597, 696, 856, 939
SduI	GDGCHC	3 cut(s)	306, 1169, 2175
SfaNI	GCATC	5 cut(s)	650, 1906, 2237, 2345, 2365
SinI	GGWCC	2 cut(s)	1788, 2290
SmiMI	CAYNNNNRTG	3 cut(s)	978, 1226, 1390
SmlI	CTYRAG	2 cut(s)	616, 1864
SmoI	CTYRAG	2 cut(s)	616, 1864
SpeI	ACTAGT	1 cut(s)	328
SsiI	CCGC	4 cut(s)	385, 645, 788, 879
SspI	AATATT	2 cut(s)	1906, 1962
SspMI	CTAG	9 cut(s)	198, 234, 329, 446, 1358, 1733, 1899, 1913, 1956
SstI	GAGCTC	1 cut(s)	2175
StyD4I	CCNGG	8 cut(s)	92, 187, 456, 520, 595, 694, 854, 937
StyI	CCWWGG	1 cut(s)	1537
TaaI	ACNGT	5 cut(s)	9, 775, 1276, 1347, 1788
TaiI	ACGT	2 cut(s)	1260, 2164
TaqI	TCGA	7 cut(s)	671, 1006, 1079, 1479, 1629, 2210, 2461
TaqII	GACCGA	1 cut(s)	2307
TatI	WGTACW	4 cut(s)	660, 1145, 1230, 2272
TfiI	GAWTC	8 cut(s)	89, 782, 970, 1678, 1843, 2123, 2207, 2282
Tru1I	TTAA	6 cut(s)	402, 515, 569, 617, 1062, 1224
Tru9I	TTAA	6 cut(s)	402, 515, 569, 617, 1062, 1224
TscAI	CASTG	5 cut(s)	12, 780, 1159, 1174, 2220
TseFI	GTSAC	5 cut(s)	582, 712, 860, 1605, 2022
TseI	GCWGC	2 cut(s)	1283, 1499
Tsp45I	GTSAC	5 cut(s)	582, 712, 860, 1605, 2022
TspGWI	ACGGA	1 cut(s)	1983
TspRI	CASTG	5 cut(s)	12, 780, 1159, 1174, 2220
Van91I	CCANNNNNTGG	1 cut(s)	108
Vha464I	CTTAAG	1 cut(s)	616
VpaK11BI	GGWCC	2 cut(s)	1788, 2290
XapI	RAATTY	4 cut(s)	193, 216, 1634, 1935
XbaI	TCTAGA	1 cut(s)	1912
XceI	RCATGY	3 cut(s)	1389, 1744, 2023
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1544
XmiI	GTMKAC	1 cut(s)	371
XmnI	GAANNNNTTC	1 cut(s)	2050
XspI	CTAG	9 cut(s)	198, 234, 329, 446, 1358, 1733, 1899, 1913, 1956
Zsp2I	ATGCAT	1 cut(s)	2252

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.