Rmu_sc0000061.1_g000037

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000061.1
OG0003570
Physical Location & Seq
Forward (+)
165814 .. 169348
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1194 bp
atgagaattttggttagggctcagcgtctgaaacgctatttgaagcaagttgatgattgcaacatcacctggggcgccatcaccatgatggacgtcatccatggcattatggatagggactatgccagccctaggctggtggtggacggaggcggtgccttaggcagcagcgtcgattcaatcaaatcattatctcccaggaaaaaggatgccccagagaggagcacactggtcgtttgcttcggtgagatgttgattgattttgtcccaacgagtgctggagtttctcttgctgatgcagaaggtttcaagaaagctgctggtggagctcctgctaatgttgcagttggaatctcaagattaggaggctcggcagcttttatgggcaaggttggtgcagatgagtttggatacatgctgtctaatattctgaaggaaaacaatgtggacaattccggcatgtgttttgaccaacatgcaaggactgcattggcttttgttacactgagaagtgatggagaacgcgaattcatgttctatcgcaacccgagtgctgatatgcttctcactaaggaagaacttaacgtcgacctaatcaaaaaggcaaatatatttcactatgggtcgattagtctgattgaggaaccatgtaaatcagcacatctcgctgcgatggacgttgctaagaaggctggctgtatcctctcttatgacccgaacttgagattgccactgtggccttctgcagaggctgctcagaagggcattatgagcatttggaacctagctgacattattaaaataagtgaggaggaaattacatttttgactggaggtgatgatccttatgatgatgatgtggtcttgaacaagctttatcaccccaatctgaagcttctgttagtaactgaagggccagcaggctgcagatactacacaaaggaattcaagggtaaagttccaggaatcaaagcggactgtgtagatacaactggagctggtgactcttttgttggctctgtactcaacagcttggcttcagaccctgatttgttcaagaatgaacaacggttgaaggaggctctcaaatttgccaatgcttgtggtgctatcactgtggaaaagaaaggagccattcctgccctacccacaaaagaagctgtccataaagttcttagccaaacatcggcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100
Pfam Domains
Protein Families
PfkB

Protein Analysis

397

Amino Acids

42.89

Weight (kDa)

5.95

Isoelectric Point (pI)

31.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 76 - 384 5.3e-77 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 96
AccB1I GGYRCC 2 cut(s) 74, 155
AccI GTMKAC 1 cut(s) 588
AccII CGCG 1 cut(s) 525
AciI CCGC 2 cut(s) 153, 974
AclWI GGATC 1 cut(s) 836
AcsI RAATTY 4 cut(s) 6, 527, 944, 1088
AcuI CTGAAG 4 cut(s) 452, 911, 930, 1023
AcyI GRCGYC 2 cut(s) 75, 93
AfaI GTAC 1 cut(s) 1023
AfiI CCNNNNNNNGG 4 cut(s) 132, 136, 219, 1186
AgsI TTSAA 7 cut(s) 43, 180, 310, 868, 949, 1057, 1075
AjnI CCWGG 3 cut(s) 68, 197, 961
AluBI AGCT 9 cut(s) 317, 329, 377, 788, 874, 895, 998, 1032, 1160
AluI AGCT 9 cut(s) 317, 329, 377, 788, 874, 895, 998, 1032, 1160
Alw21I GWGCWC 2 cut(s) 227, 331
AlwI GGATC 1 cut(s) 836
AlwNI CAGNNNCTG 3 cut(s) 28, 752, 1046
Ama87I CYCGRG 1 cut(s) 547
AoxI GGCC 2 cut(s) 737, 914
ApeKI GCWGC 7 cut(s) 165, 168, 317, 374, 668, 752, 924
ApoI RAATTY 4 cut(s) 6, 527, 944, 1088
AspA2I CCTAGG 1 cut(s) 131
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 914
AsuHPI GGTGA 6 cut(s) 58, 73, 257, 848, 872, 1013
AvaI CYCGRG 1 cut(s) 547
AvrII CCTAGG 1 cut(s) 131
AxyI CCTNAGG 1 cut(s) 160
BanI GGYRCC 2 cut(s) 74, 155
BanII GRGCYC 2 cut(s) 22, 331
Bbv12I GWGCWC 2 cut(s) 227, 331
BbvI GCAGC 7 cut(s) 177, 180, 304, 386, 655, 739, 911
BccI CCATC 4 cut(s) 82, 86, 509, 667
BcgI CGANNNNNNTGC 4 cut(s) 154, 188, 214, 248
BciT130I CCWGG 3 cut(s) 70, 199, 963
BciVI GTATCC 2 cut(s) 404, 710
BfaI CTAG 2 cut(s) 132, 785
BfmI CTRYAG 2 cut(s) 744, 925
BfoI RGCGCY 1 cut(s) 78
BfuI GTATCC 2 cut(s) 404, 710
BglI GCCNNNNNGGC 1 cut(s) 736
BisI GCNGC 7 cut(s) 166, 169, 318, 375, 669, 753, 925
BlnI CCTAGG 1 cut(s) 131
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 7 cut(s) 167, 170, 319, 376, 670, 754, 926
Bme1390I CCNGG 3 cut(s) 70, 199, 963
BmeT110I CYCGRG 1 cut(s) 547
BmgT120I GGNCC 1 cut(s) 914
BmiI GGNNCC 5 cut(s) 76, 157, 645, 782, 1132
BmrFI CCNGG 3 cut(s) 70, 199, 963
BmsI GCATC 2 cut(s) 199, 286
BpmI CTGGAG 3 cut(s) 300, 852, 1014
Bpu1102I GCTNAGC 1 cut(s) 21
BpuEI CTTGAG 2 cut(s) 340, 742
BsaHI GRCGYC 2 cut(s) 75, 93
BsaJI CCNNGG 4 cut(s) 69, 100, 131, 197
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 136, 219, 1186
Bse1I ACTGG 3 cut(s) 234, 835, 997
Bse21I CCTNAGG 1 cut(s) 160
BseBI CCWGG 3 cut(s) 70, 199, 963
BseDI CCNNGG 4 cut(s) 69, 100, 131, 197
BseGI GGATG 2 cut(s) 96, 214
BseLI CCNNNNNNNGG 4 cut(s) 132, 136, 219, 1186
BseMII CTCAG 3 cut(s) 35, 497, 770
BseNI ACTGG 3 cut(s) 234, 835, 997
BseRI GAGGAG 2 cut(s) 235, 824
BseXI GCAGC 7 cut(s) 177, 180, 304, 386, 655, 739, 911
BsgI GTGCAG 1 cut(s) 417
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 2 cut(s) 739, 916
BshNI GGYRCC 2 cut(s) 74, 155
BsiHKAI GWGCWC 2 cut(s) 227, 331
BsiHKCI CYCGRG 1 cut(s) 547
BsiSI CCGG 1 cut(s) 456
BslFI GGGAC 2 cut(s) 131, 251
BslI CCNNNNNNNGG 4 cut(s) 132, 136, 219, 1186
BsmFI GGGAC 2 cut(s) 131, 251
BsnI GGCC 2 cut(s) 739, 916
BsoBI CYCGRG 1 cut(s) 547
Bsp1286I GDGCHC 3 cut(s) 22, 227, 331
Bsp143I GATC 1 cut(s) 841
Bsp1720I GCTNAGC 1 cut(s) 21
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 2 cut(s) 153, 974
BspANI GGCC 2 cut(s) 739, 916
BspCNI CTCAG 3 cut(s) 34, 498, 769
BspFNI CGCG 1 cut(s) 525
BspLI GGNNCC 5 cut(s) 76, 157, 645, 782, 1132
BspMAI CTGCAG 2 cut(s) 748, 929
BspPI GGATC 1 cut(s) 836
BspT107I GGYRCC 2 cut(s) 74, 155
BsrI ACTGG 3 cut(s) 234, 835, 997
BssECI CCNNGG 4 cut(s) 69, 100, 131, 197
BssMI GATC 1 cut(s) 841
BssNI GRCGYC 2 cut(s) 75, 93
BssT1I CCWWGG 2 cut(s) 100, 131
Bst2UI CCWGG 3 cut(s) 70, 199, 963
Bst4CI ACNGT 4 cut(s) 735, 980, 1071, 1117
BstACI GRCGYC 2 cut(s) 75, 93
BstAPI GCANNNNNTGC 2 cut(s) 485, 752
BstC8I GCNNGC 4 cut(s) 127, 694, 918, 922
BstDEI CTNAG 7 cut(s) 21, 160, 506, 570, 684, 756, 1175
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 2 cut(s) 96, 214
BstFNI CGCG 1 cut(s) 525
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 1 cut(s) 844
BstMBI GATC 1 cut(s) 841
BstNI CCWGG 3 cut(s) 70, 199, 963
BstNSI RCATGY 3 cut(s) 418, 463, 479
BstSCI CCNGG 3 cut(s) 68, 197, 961
BstSFI CTRYAG 2 cut(s) 744, 925
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 7 cut(s) 177, 180, 304, 386, 655, 739, 911
Bsu36I CCTNAGG 1 cut(s) 160
BsuI GTATCC 2 cut(s) 404, 710
BsuRI GGCC 2 cut(s) 739, 916
BtgI CCRYGG 1 cut(s) 100
BtgZI GCGATG 1 cut(s) 686
BtsCI GGATG 2 cut(s) 96, 214
BtsIMutI CAGTG 4 cut(s) 227, 503, 731, 1113
Cac8I GCNNGC 4 cut(s) 127, 694, 918, 922
CaiI CAGNNNCTG 3 cut(s) 28, 752, 1046
CfoI GCGC 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 914
CseI GACGC 2 cut(s) 14, 160
Csp6I GTAC 1 cut(s) 1022
CspCI CAANNNNNGTGG 2 cut(s) 1084, 1119
CviAII CATG 8 cut(s) 85, 101, 415, 460, 476, 532, 648, 1191
CviQI GTAC 1 cut(s) 1022
DdeI CTNAG 7 cut(s) 21, 160, 506, 570, 684, 756, 1175
DinI GGCGCC 1 cut(s) 76
DpnI GATC 1 cut(s) 843
DpnII GATC 1 cut(s) 841
Ecl136II GAGCTC 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 100, 131
Eco24I GRGCYC 2 cut(s) 22, 331
Eco53kI GAGCTC 1 cut(s) 329
Eco57I CTGAAG 4 cut(s) 452, 911, 930, 1023
Eco81I CCTNAGG 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 547
EcoICRI GAGCTC 1 cut(s) 329
EcoRI GAATTC 2 cut(s) 527, 944
EcoRII CCWGG 3 cut(s) 68, 197, 961
EcoT14I CCWWGG 2 cut(s) 100, 131
EcoT38I GRGCYC 2 cut(s) 22, 331
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
ErhI CCWWGG 2 cut(s) 100, 131
FaeI CATG 8 cut(s) 88, 104, 418, 463, 479, 535, 651, 1194
FaqI GGGAC 2 cut(s) 131, 251
FatI CATG 8 cut(s) 84, 100, 414, 459, 475, 531, 647, 1190
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 7 cut(s) 166, 169, 318, 375, 669, 753, 925
FokI GGATG 2 cut(s) 83, 221
FriOI GRGCYC 2 cut(s) 22, 331
Fsp4HI GCNGC 7 cut(s) 166, 169, 318, 375, 669, 753, 925
FspBI CTAG 2 cut(s) 132, 785
GlaI GCGC 1 cut(s) 76
GluI GCNGC 7 cut(s) 166, 169, 318, 375, 669, 753, 925
GsuI CTGGAG 3 cut(s) 300, 852, 1014
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 2 cut(s) 739, 916
HapII CCGG 1 cut(s) 456
HgaI GACGC 2 cut(s) 14, 160
HhaI GCGC 1 cut(s) 77
Hin1I GRCGYC 2 cut(s) 75, 93
Hin1II CATG 8 cut(s) 88, 104, 418, 463, 479, 535, 651, 1194
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 2 cut(s) 872, 893
HinfI GANTC 4 cut(s) 176, 351, 966, 1004
HpaII CCGG 1 cut(s) 456
HphI GGTGA 6 cut(s) 58, 73, 257, 848, 872, 1013
Hpy166II GTNNAC 3 cut(s) 145, 448, 589
Hpy188I TCNGA 6 cut(s) 30, 432, 636, 759, 891, 1042
Hpy188III TCNNGA 4 cut(s) 310, 357, 865, 1057
Hpy8I GTNNAC 3 cut(s) 145, 448, 589
Hpy99I CGWCG 2 cut(s) 176, 590
HpyAV CCTTC 7 cut(s) 296, 427, 682, 750, 754, 905, 1069
HpyCH4III ACNGT 4 cut(s) 735, 980, 1071, 1117
HpyCH4IV ACGT 3 cut(s) 93, 585, 678
HpyCH4V TGCA 8 cut(s) 60, 299, 344, 398, 479, 488, 746, 927
HpyF3I CTNAG 7 cut(s) 21, 160, 506, 570, 684, 756, 1175
HpySE526I ACGT 3 cut(s) 93, 585, 678
Hsp92I GRCGYC 2 cut(s) 75, 93
Hsp92II CATG 8 cut(s) 88, 104, 418, 463, 479, 535, 651, 1194
HspAI GCGC 1 cut(s) 75
KasI GGCGCC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 841
LmnI GCTCC 5 cut(s) 222, 326, 334, 995, 1130
Lsp1109I GCAGC 7 cut(s) 177, 180, 304, 386, 655, 739, 911
LweI GCATC 2 cut(s) 199, 286
MaeI CTAG 2 cut(s) 132, 785
MaeII ACGT 3 cut(s) 93, 585, 678
MaeIII GTNAC 3 cut(s) 499, 904, 1001
MalI GATC 1 cut(s) 843
MboI GATC 1 cut(s) 841
MboII GAAGA 1 cut(s) 587
MhlI GDGCHC 3 cut(s) 22, 227, 331
MluCI AATT 6 cut(s) 6, 451, 527, 816, 944, 1088
Mly113I GGCGCC 1 cut(s) 75
MlyI GAGTC 1 cut(s) 998
MmeI TCCRAC 1 cut(s) 328
MseI TTAA 2 cut(s) 582, 798
MslI CAYNNNNRTG 3 cut(s) 83, 86, 1189
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 3 cut(s) 70, 199, 963
MvaI CCWGG 3 cut(s) 70, 199, 963
MvnI CGCG 1 cut(s) 525
NarI GGCGCC 1 cut(s) 75
NcoI CCATGG 1 cut(s) 100
NdeII GATC 1 cut(s) 841
NlaIII CATG 8 cut(s) 88, 104, 418, 463, 479, 535, 651, 1194
NlaIV GGNNCC 5 cut(s) 76, 157, 645, 782, 1132
NmeAIII GCCGAG 1 cut(s) 350
NmuCI GTSAC 1 cut(s) 1001
NspI RCATGY 3 cut(s) 418, 463, 479
PfeI GAWTC 3 cut(s) 176, 351, 966
PfoI TCCNGGA 1 cut(s) 961
PkrI GCNGC 7 cut(s) 167, 170, 319, 376, 670, 754, 926
PleI GAGTC 1 cut(s) 998
PluTI GGCGCC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 998
Psp124BI GAGCTC 1 cut(s) 331
Psp6I CCWGG 3 cut(s) 68, 197, 961
PspGI CCWGG 3 cut(s) 68, 197, 961
PspN4I GGNNCC 5 cut(s) 76, 157, 645, 782, 1132
PspPI GGNCC 1 cut(s) 914
PstI CTGCAG 2 cut(s) 748, 929
PstNI CAGNNNCTG 3 cut(s) 28, 752, 1046
RsaI GTAC 1 cut(s) 1023
RsaNI GTAC 1 cut(s) 1022
RseI CAYNNNNRTG 3 cut(s) 83, 86, 1189
SacI GAGCTC 1 cut(s) 331
SalI GTCGAC 1 cut(s) 587
SaqAI TTAA 2 cut(s) 582, 798
SatI GCNGC 7 cut(s) 166, 169, 318, 375, 669, 753, 925
Sau3AI GATC 1 cut(s) 841
Sau96I GGNCC 1 cut(s) 914
SchI GAGTC 1 cut(s) 998
ScrFI CCNGG 3 cut(s) 70, 199, 963
SduI GDGCHC 3 cut(s) 22, 227, 331
SfaNI GCATC 2 cut(s) 199, 286
SfcI CTRYAG 2 cut(s) 744, 925
SfoI GGCGCC 1 cut(s) 76
SmiMI CAYNNNNRTG 3 cut(s) 83, 86, 1189
SmlI CTYRAG 2 cut(s) 355, 721
SmoI CTYRAG 2 cut(s) 355, 721
Sse9I AATT 6 cut(s) 6, 451, 527, 816, 944, 1088
SsiI CCGC 2 cut(s) 153, 974
SspDI GGCGCC 1 cut(s) 74
SspI AATATT 1 cut(s) 427
SspMI CTAG 2 cut(s) 132, 785
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 3 cut(s) 68, 197, 961
StyI CCWWGG 2 cut(s) 100, 131
TaaI ACNGT 4 cut(s) 735, 980, 1071, 1117
TaiI ACGT 3 cut(s) 96, 588, 681
TaqI TCGA 3 cut(s) 174, 588, 626
TasI AATT 6 cut(s) 6, 451, 527, 816, 944, 1088
TatI WGTACW 1 cut(s) 1021
TfiI GAWTC 3 cut(s) 176, 351, 966
Tru1I TTAA 2 cut(s) 582, 798
Tru9I TTAA 2 cut(s) 582, 798
TscAI CASTG 4 cut(s) 234, 510, 738, 1120
TseFI GTSAC 1 cut(s) 1001
TseI GCWGC 7 cut(s) 165, 168, 317, 374, 668, 752, 924
Tsp45I GTSAC 1 cut(s) 1001
TspDTI ATGAA 2 cut(s) 520, 1077
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 4 cut(s) 234, 510, 738, 1120
XapI RAATTY 4 cut(s) 6, 527, 944, 1088
XceI RCATGY 3 cut(s) 418, 463, 479
XcmI CCANNNNNNNNNTGG 2 cut(s) 85, 133
XmaJI CCTAGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 588
XspI CTAG 2 cut(s) 132, 785
ZraI GACGTC 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.