Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000278.1

Orthogroup ID

OG0006113

Physical Location & Seq

Genomic Coordinates Reverse (-)

22121 .. 24067

Gene Structure 1947 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1947 bp

atggcggccattgctccactctcttcctccttgtcgaaatcgaaaccctcggctttcccttcttcttcttcttcaacaaatgccatttccagatgtactttccgccccttcccgcccattacccaccggcctctgcacatttctagttccctccctcctaaagccgcgactgccccttcggactcacaaagtttcacttcccgatttgcccctgacgagcccagaaaaggctccgatgtcctcgtggaggccctcgaacgccaaggcgtcaccgacgtattcgcctaccccggaggcgcatccatggagatccatcaggccctcacgcgctcctccaccatccgcaatgttctcccccgccacgagcaaggtggcatcttcgctgctgagggctacgcgcgcgcctcgggccttcccggcgtctgtattgcaacctcgggccctggcgctaccaacttagtcagtggccttgctgacgcactccttgacagcgttccggtcgtcgctatcaccggacaagtcccccgccggatgatcggcaccgacgcttttcaagagaccccaattgtagaggtaaccaggtccattacaaagcataactatcttgttcttgatgttgaagaaattcctagaattgttagtgaggcatttttcttggccacctccggccgccccggcccagttttgatagatataccaaaagacatacagcaacagcttgttgttccggattggaatcaacccattagattagcggggtacctgtctaggctgcccaatcctcccaatgaggcccatttggagcagattgtaaggttggtttccgaatccaagaagcctgtgttgtatgttggtggagggtgcttgaactcgagtgaggaattgaggcgatttgtggagtttactgggatccctgtcgcgactaccttgatgggtctcggggcatatcctagctcagatgagctgtcactccaaatgttgggaatgcatgggacagtgcatgctaattatgcagtggataagtctgacttgttgcttgcatttggggttaggtttgatgaccgggttacagggaagctcgaagcttttgctagccgagctaagattgttcacattgatattgattctgcagagattggaaagaataagcagcctcatgtatctgtttgtgcagatgtgagattggcattgggagggataaacagggtattggagggaaaaggatacaagttcaaacttgatttctcagcctggagggaggagctgaaagagcagaaaattagttatccattaaattataagacatttggtgacgccattcctcctcagtgtgctattcaggttcttgatgaattaactgatgggaatgcaatcataagcaccggtgtcgggcagcatcaaatgtgggcagctcaattttacaaatataagaggcctagacagtggttgacatcaggaggtttaggagctatgggttttggattgcctgctgctattggggcagctgtggcaaatccagatgccattgttgttgatattgatggtgatggaagcttcatgatgaatgttcaggaattagcaactatcagggtggaaaatcttcccattaagataatgctgttgaataatcagcatttgggtatggttgttcaatgggaggatcgcttctataaggcgaatagggctcacacttatttagggaacccattaaatgagtctgagatattccctaatatgctcaagttcgcagaagcttgtgggataccagctgcccgtgtgacaaagaaggaacatcttaaagaggcaattcagaaaatgttggacactcctggtccgtacttgttggatgtagtagtacctcatcaagagcatgtcttgcccatgattccgagtggtggtgcttttaaagatgtgataactgagggtgatggaagatcttcttattga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009099 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

648

Amino Acids

70.25

Weight (kDa)

6.37

Isoelectric Point (pI)

40.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	76 - 186	1.9e-36	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	267 - 397	3.1e-45	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	462 - 617	3.4e-52	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48560
fragaria_vesca	FvH4_7g06770
malus_domestica	MD06G1028700.v1.1 MD10G1013700.v1.1
prunus_persica	Prupe.5G043100_v2.0.a1 Prupe.8G001300_v2.0.a1 Prupe.8G001300_v2.0.a1
pyrus_communis	pycom06g02280 pycom10g00840
rosa_chinensis	RchiOBHm_Chr7g0208791
rosa_laevigata	RLG00000003195 RLG00000015583
rosa_multiflora	Rmu_co8493263.1_g000001 Rmu_sc0000278.1_g000002 Rmu_sc0008663.1_g000001 Rmu_sc0018750.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250260 Rroxscaffold_7G00217760
rosa_rugosa	Rorug05G0492000 Rorug07G0105800
rosa_samantha	Rh6AG001800 Rh7AG241000 Rh7BG235900 Rh7CG255600 Rh7DG247000
rosa_wichuraiana	Rw6G000240 Rw7G020440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1299
Acc65I	GGTACC	1 cut(s)	759
AccB1I	GGYRCC	2 cut(s)	539, 759
AccB7I	CCANNNNNTGG	1 cut(s)	979
AccII	CGCG	6 cut(s)	167, 328, 398, 400, 402, 920
AccIII	TCCGGA	1 cut(s)	727
AciI	CCGC	9 cut(s)	5, 103, 113, 165, 343, 358, 526, 670, 755
AclWI	GGATC	4 cut(s)	304, 904, 917, 1668
AcoI	YGGCCR	3 cut(s)	6, 657, 667
AcsI	RAATTY	1 cut(s)	624
AcyI	GRCGYC	3 cut(s)	267, 420, 1314
AfaI	GTAC	4 cut(s)	97, 761, 1838, 1857
AfiI	CCNNNNNNNGG	5 cut(s)	227, 247, 979, 1389, 1895
AgeI	ACCGGT	1 cut(s)	1382
AgsI	TTSAA	7 cut(s)	75, 554, 620, 868, 1234, 1624, 1652
AjnI	CCWGG	4 cut(s)	442, 578, 1250, 1828
Alw26I	GTCTC	2 cut(s)	551, 941
AlwI	GGATC	4 cut(s)	304, 904, 917, 1668
Ama87I	CYCGRG	4 cut(s)	406, 436, 871, 938
Aor13HI	TCCGGA	1 cut(s)	727
ApaI	GGGCCC	1 cut(s)	443
ApeKI	GCWGC	8 cut(s)	383, 772, 1150, 1393, 1409, 1490, 1502, 1769
ApoI	RAATTY	1 cut(s)	624
ArsI	GACNNNNNNTTYG	2 cut(s)	160, 192
AsiGI	ACCGGT	1 cut(s)	1382
Asp700I	GAANNNNTTC	3 cut(s)	624, 1599, 1936
Asp718I	GGTACC	1 cut(s)	759
AspLEI	GCGC	6 cut(s)	299, 330, 400, 402, 404, 449
AspS9I	GGNCC	9 cut(s)	250, 319, 409, 439, 440, 582, 677, 793, 1832
AsuC2I	CCSGG	4 cut(s)	291, 417, 675, 1064
AsuHPI	GGTGA	5 cut(s)	262, 502, 1322, 1556, 1937
AsuNHI	GCTAGC	1 cut(s)	1091
AvaI	CYCGRG	4 cut(s)	406, 436, 871, 938
AvaII	GGWCC	2 cut(s)	582, 1832
BaeGI	GKGCMC	1 cut(s)	443
BalI	TGGCCA	1 cut(s)	659
BamHI	GGATCC	1 cut(s)	909
BanI	GGYRCC	2 cut(s)	539, 759
BanII	GRGCYC	3 cut(s)	222, 443, 1687
BauI	CACGAG	2 cut(s)	242, 362
BbvCI	CCTCAGC	1 cut(s)	387
BbvI	GCAGC	8 cut(s)	370, 759, 1162, 1405, 1421, 1477, 1514, 1756
BccI	CCATC	7 cut(s)	321, 347, 925, 1355, 1535, 1541, 1922
BcgI	CGANNNNNNTGC	4 cut(s)	1070, 1104, 1369, 1403
BciT130I	CCWGG	4 cut(s)	444, 580, 1252, 1830
BciVI	GTATCC	2 cut(s)	1217, 1755
BcnI	CCSGG	4 cut(s)	291, 417, 675, 1064
BcoDI	GTCTC	2 cut(s)	551, 941
BfaI	CTAG	6 cut(s)	144, 630, 768, 951, 1092, 1437
BfmI	CTRYAG	1 cut(s)	1128
BfoI	RGCGCY	1 cut(s)	450
BfuI	GTATCC	2 cut(s)	1217, 1755
BglI	GCCNNNNNGGC	2 cut(s)	417, 675
BglII	AGATCT	1 cut(s)	1934
Bme1390I	CCNGG	8 cut(s)	291, 417, 444, 580, 675, 1064, 1252, 1830
Bme18I	GGWCC	2 cut(s)	582, 1832
BmeT110I	CYCGRG	4 cut(s)	406, 436, 871, 938
BmgT120I	GGNCC	9 cut(s)	250, 319, 409, 439, 440, 582, 677, 793, 1832
BmiI	GGNNCC	6 cut(s)	232, 441, 541, 761, 911, 1703
BmrFI	CCNGG	8 cut(s)	291, 417, 444, 580, 675, 1064, 1252, 1830
BmrI	ACTGGG	2 cut(s)	674, 915
BmsI	GCATC	4 cut(s)	308, 384, 1405, 1510
BmtI	GCTAGC	1 cut(s)	1095
BmuI	ACTGGG	2 cut(s)	674, 915
BpmI	CTGGAG	1 cut(s)	1273
Bpu10I	CCTNAGC	1 cut(s)	387
BpuEI	CTTGAG	1 cut(s)	1724
BpuMI	CCSGG	4 cut(s)	291, 417, 675, 1064
BsaBI	GATNNNNATC	1 cut(s)	735
BsaHI	GRCGYC	3 cut(s)	267, 420, 1314
BsaI	GGTCTC	2 cut(s)	551, 941
BsaJI	CCNNGG	8 cut(s)	48, 262, 289, 303, 405, 435, 442, 673
BsaWI	WCCGGW	4 cut(s)	496, 512, 727, 1382
Bsc4I	CCNNNNNNNGG	5 cut(s)	227, 247, 979, 1389, 1895
Bse118I	RCCGGY	2 cut(s)	126, 1382
Bse1I	ACTGG	2 cut(s)	680, 910
Bse3DI	GCAATG	2 cut(s)	9, 352
Bse8I	GATNNNNATC	1 cut(s)	735
BseAI	TCCGGA	1 cut(s)	727
BseBI	CCWGG	4 cut(s)	444, 580, 1252, 1830
BseDI	CCNNGG	8 cut(s)	48, 262, 289, 303, 405, 435, 442, 673
BseGI	GGATG	4 cut(s)	299, 339, 537, 1852
BseJI	GATNNNNATC	1 cut(s)	735
BseLI	CCNNNNNNNGG	5 cut(s)	227, 247, 979, 1389, 1895
BseMI	GCAATG	2 cut(s)	9, 352
BseMII	CTCAG	6 cut(s)	378, 969, 1260, 1340, 1710, 1911
BseNI	ACTGG	2 cut(s)	680, 910
BsePI	GCGCGC	2 cut(s)	398, 400
BseRI	GAGGAG	3 cut(s)	322, 1274, 1314
BseSI	GKGCMC	1 cut(s)	443
BseX3I	CGGCCG	1 cut(s)	667
BseXI	GCAGC	8 cut(s)	370, 759, 1162, 1405, 1421, 1477, 1514, 1756
BsgI	GTGCAG	2 cut(s)	119, 1191
Bsh1236I	CGCG	6 cut(s)	167, 328, 398, 400, 402, 920
Bsh1285I	CGRYCG	2 cut(s)	501, 670
BshNI	GGYRCC	2 cut(s)	539, 759
BshTI	ACCGGT	1 cut(s)	1382
BsiEI	CGRYCG	2 cut(s)	501, 670
BsiHKCI	CYCGRG	4 cut(s)	406, 436, 871, 938
BslFI	GGGAC	2 cut(s)	506, 1006
BslI	CCNNNNNNNGG	5 cut(s)	227, 247, 979, 1389, 1895
BsmAI	GTCTC	2 cut(s)	551, 941
BsmFI	GGGAC	2 cut(s)	506, 1006
BsmI	GAATGC	2 cut(s)	990, 1372
Bso31I	GGTCTC	2 cut(s)	551, 941
BsoBI	CYCGRG	4 cut(s)	406, 436, 871, 938
Bsp120I	GGGCCC	1 cut(s)	439
Bsp1286I	GDGCHC	3 cut(s)	222, 443, 1687
Bsp13I	TCCGGA	1 cut(s)	727
Bsp143I	GATC	5 cut(s)	309, 534, 909, 1660, 1934
Bsp19I	CCATGG	1 cut(s)	303
Bsp68I	TCGCGA	1 cut(s)	920
BspACI	CCGC	9 cut(s)	5, 103, 113, 165, 343, 358, 526, 670, 755
BspCNI	CTCAG	6 cut(s)	379, 968, 1259, 1339, 1711, 1912
BspEI	TCCGGA	1 cut(s)	727
BspFNI	CGCG	6 cut(s)	167, 328, 398, 400, 402, 920
BspHI	TCATGA	1 cut(s)	1557
BspLI	GGNNCC	6 cut(s)	232, 441, 541, 761, 911, 1703
BspMAI	CTGCAG	1 cut(s)	1132
BspOI	GCTAGC	1 cut(s)	1095
BspPI	GGATC	4 cut(s)	304, 904, 917, 1668
BspT107I	GGYRCC	2 cut(s)	539, 759
BspTNI	GGTCTC	2 cut(s)	551, 941
BsrDI	GCAATG	2 cut(s)	9, 352
BsrFI	RCCGGY	2 cut(s)	126, 1382
BsrI	ACTGG	2 cut(s)	680, 910
BssAI	RCCGGY	2 cut(s)	126, 1382
BssECI	CCNNGG	8 cut(s)	48, 262, 289, 303, 405, 435, 442, 673
BssHII	GCGCGC	2 cut(s)	398, 400
BssMI	GATC	5 cut(s)	309, 534, 909, 1660, 1934
BssNI	GRCGYC	3 cut(s)	267, 420, 1314
BssSI	CACGAG	2 cut(s)	242, 362
BssT1I	CCWWGG	2 cut(s)	262, 303
Bst2BI	CACGAG	2 cut(s)	242, 362
Bst2UI	CCWGG	4 cut(s)	444, 580, 1252, 1830
Bst4CI	ACNGT	2 cut(s)	997, 1443
Bst6I	CTCTTC	1 cut(s)	28
BstACI	GRCGYC	3 cut(s)	267, 420, 1314
BstAPI	GCANNNNNTGC	1 cut(s)	1876
BstC8I	GCNNGC	6 cut(s)	400, 402, 1002, 1038, 1093, 1488
BstDEI	CTNAG	8 cut(s)	387, 457, 955, 1101, 1246, 1326, 1719, 1920
BstDSI	CCRYGG	1 cut(s)	303
BstEII	GGTNACC	1 cut(s)	574
BstENI	CCTNNNNNAGG	1 cut(s)	245
BstF5I	GGATG	4 cut(s)	299, 339, 537, 1852
BstFNI	CGCG	6 cut(s)	167, 328, 398, 400, 402, 920
BstH2I	RGCGCY	1 cut(s)	450
BstHHI	GCGC	6 cut(s)	299, 330, 400, 402, 404, 449
BstKTI	GATC	5 cut(s)	312, 537, 912, 1663, 1937
BstMAI	GTCTC	2 cut(s)	551, 941
BstMBI	GATC	5 cut(s)	309, 534, 909, 1660, 1934
BstMCI	CGRYCG	2 cut(s)	501, 670
BstNI	CCWGG	4 cut(s)	444, 580, 1252, 1830
BstNSI	RCATGY	2 cut(s)	1004, 1874
BstPI	GGTNACC	1 cut(s)	574
BstSCI	CCNGG	8 cut(s)	289, 415, 442, 578, 673, 1062, 1250, 1828
BstSFI	CTRYAG	1 cut(s)	1128
BstSLI	GKGCMC	1 cut(s)	443
BstUI	CGCG	6 cut(s)	167, 328, 398, 400, 402, 920
BstV1I	GCAGC	8 cut(s)	370, 759, 1162, 1405, 1421, 1477, 1514, 1756
BstX2I	RGATCY	3 cut(s)	309, 909, 1934
BstYI	RGATCY	3 cut(s)	309, 909, 1934
BstZI	CGGCCG	1 cut(s)	667
BsuI	GTATCC	2 cut(s)	1217, 1755
BtgI	CCRYGG	1 cut(s)	303
BtsCI	GGATG	4 cut(s)	299, 339, 537, 1852
BtsI	GCAGTG	1 cut(s)	1020
BtsIMutI	CAGTG	5 cut(s)	469, 1002, 1020, 1334, 1448
BtuMI	TCGCGA	1 cut(s)	920
Cac8I	GCNNGC	6 cut(s)	400, 402, 1002, 1038, 1093, 1488
CciI	TCATGA	1 cut(s)	1557
CfoI	GCGC	6 cut(s)	299, 330, 400, 402, 404, 449
Cfr10I	RCCGGY	2 cut(s)	126, 1382
Cfr13I	GGNCC	9 cut(s)	250, 319, 409, 439, 440, 582, 677, 793, 1832
CseI	GACGC	5 cut(s)	256, 409, 485, 554, 1322
CsiI	ACCWGGT	1 cut(s)	578
Csp6I	GTAC	4 cut(s)	96, 760, 1837, 1856
CspAI	ACCGGT	1 cut(s)	1382
CviAII	CATG	7 cut(s)	304, 989, 1001, 1157, 1558, 1871, 1882
CviQI	GTAC	4 cut(s)	96, 760, 1837, 1856
DdeI	CTNAG	8 cut(s)	387, 457, 955, 1101, 1246, 1326, 1719, 1920
DpnI	GATC	5 cut(s)	311, 536, 911, 1662, 1936
DpnII	GATC	5 cut(s)	309, 534, 909, 1660, 1934
DraI	TTTAAA	1 cut(s)	1906
EaeI	YGGCCR	3 cut(s)	6, 657, 667
EagI	CGGCCG	1 cut(s)	667
Eam1104I	CTCTTC	1 cut(s)	28
EarI	CTCTTC	1 cut(s)	28
EciI	GGCGGA	1 cut(s)	92
EclXI	CGGCCG	1 cut(s)	667
Eco130I	CCWWGG	2 cut(s)	262, 303
Eco147I	AGGCCT	1 cut(s)	1435
Eco24I	GRGCYC	3 cut(s)	222, 443, 1687
Eco31I	GGTCTC	2 cut(s)	551, 941
Eco47I	GGWCC	2 cut(s)	582, 1832
Eco52I	CGGCCG	1 cut(s)	667
Eco88I	CYCGRG	4 cut(s)	406, 436, 871, 938
Eco91I	GGTNACC	1 cut(s)	574
EcoNI	CCTNNNNNAGG	1 cut(s)	245
EcoO109I	RGGNCCY	3 cut(s)	250, 319, 440
EcoO65I	GGTNACC	1 cut(s)	574
EcoRII	CCWGG	4 cut(s)	442, 578, 1250, 1828
EcoT14I	CCWWGG	2 cut(s)	262, 303
EcoT22I	ATGCAT	1 cut(s)	990
EcoT38I	GRGCYC	3 cut(s)	222, 443, 1687
ErhI	CCWWGG	2 cut(s)	262, 303
FaeI	CATG	7 cut(s)	307, 992, 1004, 1160, 1561, 1874, 1885
FalI	AAGNNNNNCTT	5 cut(s)	181, 213, 1013, 1045, 1924
FaqI	GGGAC	2 cut(s)	506, 1006
FatI	CATG	7 cut(s)	303, 988, 1000, 1156, 1557, 1870, 1881
FauI	CCCGC	4 cut(s)	120, 365, 533, 748
FokI	GGATG	4 cut(s)	286, 326, 544, 1859
FriOI	GRGCYC	3 cut(s)	222, 443, 1687
FspBI	CTAG	6 cut(s)	144, 630, 768, 951, 1092, 1437
GlaI	GCGC	6 cut(s)	298, 329, 399, 401, 403, 448
GsuI	CTGGAG	1 cut(s)	1273
HaeII	RGCGCY	1 cut(s)	450
HgaI	GACGC	5 cut(s)	256, 409, 485, 554, 1322
HhaI	GCGC	6 cut(s)	299, 330, 400, 402, 404, 449
Hin1I	GRCGYC	3 cut(s)	267, 420, 1314
Hin1II	CATG	7 cut(s)	307, 992, 1004, 1160, 1561, 1874, 1885
Hin6I	GCGC	6 cut(s)	297, 328, 398, 400, 402, 447
HinP1I	GCGC	6 cut(s)	297, 328, 398, 400, 402, 447
HincII	GTYRAC	1 cut(s)	1449
HindII	GTYRAC	1 cut(s)	1449
HindIII	AAGCTT	3 cut(s)	1083, 1552, 1752
HinfI	GANTC	6 cut(s)	182, 736, 827, 1124, 1715, 1885
HphI	GGTGA	5 cut(s)	262, 502, 1322, 1556, 1937
Hpy166II	GTNNAC	3 cut(s)	903, 1111, 1449
Hpy188I	TCNGA	8 cut(s)	181, 235, 826, 958, 1027, 1720, 1812, 1890
Hpy8I	GTNNAC	3 cut(s)	903, 1111, 1449
Hpy99I	CGWCG	3 cut(s)	278, 506, 548
HpyAV	CCTTC	5 cut(s)	69, 118, 186, 422, 1780
HpyCH4III	ACNGT	2 cut(s)	997, 1443
HpyCH4IV	ACGT	1 cut(s)	276
HpyCH4V	TGCA	9 cut(s)	136, 431, 988, 1000, 1013, 1040, 1130, 1172, 1370
HpyF3I	CTNAG	8 cut(s)	387, 457, 955, 1101, 1246, 1326, 1719, 1920
HpySE526I	ACGT	1 cut(s)	276
Hsp92I	GRCGYC	3 cut(s)	267, 420, 1314
Hsp92II	CATG	7 cut(s)	307, 992, 1004, 1160, 1561, 1874, 1885
HspAI	GCGC	6 cut(s)	297, 328, 398, 400, 402, 447
Kpn2I	TCCGGA	1 cut(s)	727
KpnI	GGTACC	1 cut(s)	763
Kzo9I	GATC	5 cut(s)	309, 534, 909, 1660, 1934
LmnI	GCTCC	6 cut(s)	19, 236, 335, 802, 1261, 1466
Lsp1109I	GCAGC	8 cut(s)	370, 759, 1162, 1405, 1421, 1477, 1514, 1756
LweI	GCATC	4 cut(s)	308, 384, 1405, 1510
MabI	ACCWGGT	1 cut(s)	578
MaeI	CTAG	6 cut(s)	144, 630, 768, 951, 1092, 1437
MaeII	ACGT	1 cut(s)	276
MaeIII	GTNAC	6 cut(s)	268, 574, 966, 1066, 1310, 1777
MalI	GATC	5 cut(s)	311, 536, 911, 1662, 1936
MauBI	CGCGCGCG	1 cut(s)	398
MboI	GATC	5 cut(s)	309, 534, 909, 1660, 1934
MfeI	CAATTG	1 cut(s)	564
MflI	RGATCY	3 cut(s)	309, 909, 1934
MhlI	GDGCHC	3 cut(s)	222, 443, 1687
MlsI	TGGCCA	1 cut(s)	659
MluNI	TGGCCA	1 cut(s)	659
MlyI	GAGTC	2 cut(s)	176, 1724
MmeI	TCCRAC	2 cut(s)	1800, 1824
Mox20I	TGGCCA	1 cut(s)	659
Mph1103I	ATGCAT	1 cut(s)	990
MroI	TCCGGA	1 cut(s)	727
MroXI	GAANNNNTTC	3 cut(s)	624, 1599, 1936
MscI	TGGCCA	1 cut(s)	659
MseI	TTAA	6 cut(s)	1292, 1355, 1608, 1709, 1797, 1905
Msp20I	TGGCCA	1 cut(s)	659
MspA1I	CMGCKG	2 cut(s)	1505, 1769
MspR9I	CCNGG	8 cut(s)	291, 417, 444, 580, 675, 1064, 1252, 1830
MunI	CAATTG	1 cut(s)	564
Mva1269I	GAATGC	2 cut(s)	990, 1372
MvaI	CCWGG	4 cut(s)	444, 580, 1252, 1830
MvnI	CGCG	6 cut(s)	167, 328, 398, 400, 402, 920
NciI	CCSGG	4 cut(s)	291, 417, 675, 1064
NcoI	CCATGG	1 cut(s)	303
NdeII	GATC	5 cut(s)	309, 534, 909, 1660, 1934
NheI	GCTAGC	1 cut(s)	1091
NlaIII	CATG	7 cut(s)	307, 992, 1004, 1160, 1561, 1874, 1885
NlaIV	GGNNCC	6 cut(s)	232, 441, 541, 761, 911, 1703
NmeAIII	GCCGAG	2 cut(s)	29, 1121
NmuCI	GTSAC	4 cut(s)	268, 966, 1310, 1777
NruI	TCGCGA	1 cut(s)	920
NsiI	ATGCAT	1 cut(s)	990
NspI	RCATGY	2 cut(s)	1004, 1874
PaeI	GCATGC	1 cut(s)	1004
PaeR7I	CTCGAG	1 cut(s)	871
PagI	TCATGA	1 cut(s)	1557
PauI	GCGCGC	2 cut(s)	398, 400
PceI	AGGCCT	1 cut(s)	1435
PctI	GAATGC	2 cut(s)	990, 1372
PdmI	GAANNNNTTC	3 cut(s)	624, 1599, 1936
PfeI	GAWTC	4 cut(s)	736, 827, 1124, 1885
PflMI	CCANNNNNTGG	1 cut(s)	979
PinAI	ACCGGT	1 cut(s)	1382
PleI	GAGTC	2 cut(s)	176, 1723
PpsI	GAGTC	2 cut(s)	176, 1723
PsiI	TTATAA	1 cut(s)	1299
Psp6I	CCWGG	4 cut(s)	442, 578, 1250, 1828
PspEI	GGTNACC	1 cut(s)	574
PspGI	CCWGG	4 cut(s)	442, 578, 1250, 1828
PspN4I	GGNNCC	6 cut(s)	232, 441, 541, 761, 911, 1703
PspOMI	GGGCCC	1 cut(s)	439
PspPI	GGNCC	9 cut(s)	250, 319, 409, 439, 440, 582, 677, 793, 1832
PspXI	VCTCGAGB	1 cut(s)	871
PstI	CTGCAG	1 cut(s)	1132
PsuI	RGATCY	3 cut(s)	309, 909, 1934
PteI	GCGCGC	2 cut(s)	398, 400
PvuII	CAGCTG	2 cut(s)	1505, 1769
RruI	TCGCGA	1 cut(s)	920
RsaI	GTAC	4 cut(s)	97, 761, 1838, 1857
RsaNI	GTAC	4 cut(s)	96, 760, 1837, 1856
SaqAI	TTAA	6 cut(s)	1292, 1355, 1608, 1709, 1797, 1905
Sau3AI	GATC	5 cut(s)	309, 534, 909, 1660, 1934
Sau96I	GGNCC	9 cut(s)	250, 319, 409, 439, 440, 582, 677, 793, 1832
SchI	GAGTC	2 cut(s)	176, 1724
ScrFI	CCNGG	8 cut(s)	291, 417, 444, 580, 675, 1064, 1252, 1830
SduI	GDGCHC	3 cut(s)	222, 443, 1687
SexAI	ACCWGGT	1 cut(s)	578
SfaNI	GCATC	4 cut(s)	308, 384, 1405, 1510
SfcI	CTRYAG	1 cut(s)	1128
SfiI	GGCCNNNNNGGCC	1 cut(s)	675
Sfr274I	CTCGAG	1 cut(s)	871
SgrAI	CRCCGGYG	1 cut(s)	1382
SinI	GGWCC	2 cut(s)	582, 1832
SlaI	CTCGAG	1 cut(s)	871
SmlI	CTYRAG	2 cut(s)	871, 1739
SmoI	CTYRAG	2 cut(s)	871, 1739
SphI	GCATGC	1 cut(s)	1004
SseBI	AGGCCT	1 cut(s)	1435
SsiI	CCGC	9 cut(s)	5, 103, 113, 165, 343, 358, 526, 670, 755
SspMI	CTAG	6 cut(s)	144, 630, 768, 951, 1092, 1437
StuI	AGGCCT	1 cut(s)	1435
StyD4I	CCNGG	8 cut(s)	289, 415, 442, 578, 673, 1062, 1250, 1828
StyI	CCWWGG	2 cut(s)	262, 303
TaaI	ACNGT	2 cut(s)	997, 1443
TaiI	ACGT	1 cut(s)	279
TaqI	TCGA	5 cut(s)	35, 41, 255, 872, 1080
TatI	WGTACW	1 cut(s)	95
TauI	GCSGC	3 cut(s)	8, 167, 672
TfiI	GAWTC	4 cut(s)	736, 827, 1124, 1885
Tru1I	TTAA	6 cut(s)	1292, 1355, 1608, 1709, 1797, 1905
Tru9I	TTAA	6 cut(s)	1292, 1355, 1608, 1709, 1797, 1905
TscAI	CASTG	5 cut(s)	469, 1002, 1020, 1334, 1448
TseFI	GTSAC	4 cut(s)	268, 966, 1310, 1777
TseI	GCWGC	8 cut(s)	383, 772, 1150, 1393, 1409, 1490, 1502, 1769
Tsp45I	GTSAC	4 cut(s)	268, 966, 1310, 1777
TspDTI	ATGAA	3 cut(s)	1365, 1546, 1577
TspGWI	ACGGA	1 cut(s)	1824
TspRI	CASTG	5 cut(s)	469, 1002, 1020, 1334, 1448
Van91I	CCANNNNNTGG	1 cut(s)	979
VpaK11BI	GGWCC	2 cut(s)	582, 1832
XagI	CCTNNNNNAGG	1 cut(s)	245
XapI	RAATTY	1 cut(s)	624
XceI	RCATGY	2 cut(s)	1004, 1874
XcmI	CCANNNNNNNNNTGG	1 cut(s)	368
XhoI	CTCGAG	1 cut(s)	871
XmnI	GAANNNNTTC	3 cut(s)	624, 1599, 1936
XspI	CTAG	6 cut(s)	144, 630, 768, 951, 1092, 1437
Zsp2I	ATGCAT	1 cut(s)	990

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.