Rmu_sc0000735.1_g000021

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000735.1
OG0004601
Physical Location & Seq
Reverse (-)
101057 .. 102439
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 426 bp
atgaaggcatcatatgtcgcaattgcattttgcactgtgcttgtggtgatgctggccacagaagaggtgtcggtggtagctgtgacttgcaacccggcagagctgagctcttgtgcaaatgcaataagctctaatactgcaccatcaaatctatgctgcaccaaactcaatcagcagaagccttgtctctgccagtacctcaacaaccccaacctgaagcaattcgttaacagcccgggcgctcggaatgttgctagggcttgtggagtgccctttcccagagggagaagtcatcctccaccttgccggacttcggtgaccagtgggctgccgccgactccggaatccggaatccggaatcctgctaccgaactggtgaccggattccggagtctgaatttattgcctcctaataatacccagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

141

Amino Acids

14.91

Weight (kDa)

8.99

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 33 - 94 8.9e-09 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 4 cut(s) 340, 347, 354, 387
AciI CCGC 1 cut(s) 332
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 236
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 4 cut(s) 313, 347, 354, 387
AluBI AGCT 4 cut(s) 80, 103, 108, 129
AluI AGCT 4 cut(s) 80, 103, 108, 129
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 235
Aor13HI TCCGGA 4 cut(s) 340, 347, 354, 387
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 2 cut(s) 156, 328
ApoI RAATTY 1 cut(s) 397
Asp700I GAANNNNTTC 1 cut(s) 221
AspLEI GCGC 1 cut(s) 242
AsuC2I CCSGG 3 cut(s) 95, 236, 237
AsuHPI GGTGA 3 cut(s) 58, 328, 388
AvaI CYCGRG 1 cut(s) 235
BaeGI GKGCMC 1 cut(s) 273
BalI TGGCCA 1 cut(s) 56
BanII GRGCYC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 2 cut(s) 143, 315
BccI CCATC 1 cut(s) 151
BcnI CCSGG 3 cut(s) 95, 236, 237
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 1 cut(s) 255
BfoI RGCGCY 1 cut(s) 243
BisI GCNGC 3 cut(s) 157, 329, 332
BlpI GCTNAGC 1 cut(s) 104
BlsI GCNGC 3 cut(s) 158, 330, 333
Bme1390I CCNGG 3 cut(s) 95, 236, 237
BmeT110I CYCGRG 1 cut(s) 235
BmrFI CCNGG 3 cut(s) 95, 236, 237
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 2 cut(s) 17, 39
BmuI ACTGGG 1 cut(s) 415
BplI GAGNNNNNCTC 2 cut(s) 92, 124
Bpu1102I GCTNAGC 1 cut(s) 104
BpuMI CCSGG 3 cut(s) 95, 236, 237
BsaJI CCNNGG 1 cut(s) 235
BsaWI WCCGGW 5 cut(s) 340, 347, 354, 380, 387
Bsc4I CCNNNNNNNGG 4 cut(s) 313, 347, 354, 387
Bse1I ACTGG 4 cut(s) 193, 321, 378, 421
BseAI TCCGGA 4 cut(s) 340, 347, 354, 387
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 4 cut(s) 313, 347, 354, 387
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 4 cut(s) 193, 321, 378, 421
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 2 cut(s) 143, 315
BsgI GTGCAG 2 cut(s) 123, 142
BshFI GGCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 110
BsiHKCI CYCGRG 1 cut(s) 235
BsiSI CCGG 8 cut(s) 95, 236, 307, 341, 348, 355, 381, 388
BslI CCNNNNNNNGG 4 cut(s) 313, 347, 354, 387
BsmAI GTCTC 1 cut(s) 191
BsnI GGCC 1 cut(s) 56
BsoBI CYCGRG 1 cut(s) 235
Bsp1286I GDGCHC 2 cut(s) 110, 273
Bsp13I TCCGGA 4 cut(s) 340, 347, 354, 387
Bsp1720I GCTNAGC 1 cut(s) 104
BspACI CCGC 1 cut(s) 332
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 96
BspEI TCCGGA 4 cut(s) 340, 347, 354, 387
BsrI ACTGG 4 cut(s) 193, 321, 378, 421
BssECI CCNNGG 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 1 cut(s) 104
BstEII GGTNACC 2 cut(s) 316, 376
BstF5I GGATG 1 cut(s) 292
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 1 cut(s) 242
BstMAI GTCTC 1 cut(s) 191
BstPI GGTNACC 2 cut(s) 316, 376
BstSCI CCNGG 3 cut(s) 93, 234, 235
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 2 cut(s) 143, 315
BsuRI GGCC 1 cut(s) 56
BtsCI GGATG 1 cut(s) 292
BtsIMutI CAGTG 2 cut(s) 33, 328
Cac8I GCNNGC 1 cut(s) 54
CfoI GCGC 1 cut(s) 242
Cfr9I CCCGGG 1 cut(s) 235
Csp6I GTAC 1 cut(s) 196
CviJI RGCY 9 cut(s) 56, 80, 103, 108, 129, 181, 234, 260, 328
CviKI_1 RGCY 9 cut(s) 56, 80, 103, 108, 129, 181, 234, 260, 328
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 104
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 110
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 235
Eco91I GGTNACC 2 cut(s) 316, 376
EcoICRI GAGCTC 1 cut(s) 108
EcoO65I GGTNACC 2 cut(s) 316, 376
EcoT38I GRGCYC 1 cut(s) 110
FaiI YATR 3 cut(s) 13, 15, 154
FauNDI CATATG 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 157, 329, 332
FokI GGATG 1 cut(s) 279
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 3 cut(s) 157, 329, 332
FspBI CTAG 1 cut(s) 255
GlaI GCGC 1 cut(s) 241
GluI GCNGC 3 cut(s) 157, 329, 332
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 1 cut(s) 56
HapII CCGG 8 cut(s) 95, 236, 307, 341, 348, 355, 381, 388
HhaI GCGC 1 cut(s) 242
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 6 cut(s) 337, 344, 351, 358, 384, 391
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 8 cut(s) 95, 236, 307, 341, 348, 355, 381, 388
HphI GGTGA 3 cut(s) 58, 328, 388
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 246, 396
Hpy188III TCNNGA 4 cut(s) 341, 348, 355, 388
Hpy8I GTNNAC 1 cut(s) 229
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 7 cut(s) 26, 33, 90, 116, 122, 140, 159
HpyF3I CTNAG 1 cut(s) 104
HspAI GCGC 1 cut(s) 240
Kpn2I TCCGGA 4 cut(s) 340, 347, 354, 387
KspAI GTTAAC 1 cut(s) 229
Lsp1109I GCAGC 2 cut(s) 143, 315
LweI GCATC 2 cut(s) 17, 39
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 82, 316, 376
MboII GAAGA 1 cut(s) 74
MfeI CAATTG 1 cut(s) 21
MhlI GDGCHC 2 cut(s) 110, 273
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 3 cut(s) 21, 221, 397
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 2 cut(s) 331, 400
MnlI CCTC 5 cut(s) 58, 209, 275, 306, 417
Mox20I TGGCCA 1 cut(s) 56
MroI TCCGGA 4 cut(s) 340, 347, 354, 387
MroXI GAANNNNTTC 1 cut(s) 221
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 56
MspI CCGG 8 cut(s) 95, 236, 307, 341, 348, 355, 381, 388
MspR9I CCNGG 3 cut(s) 95, 236, 237
MunI CAATTG 1 cut(s) 21
NciI CCSGG 3 cut(s) 95, 236, 237
NdeI CATATG 1 cut(s) 13
NmuCI GTSAC 3 cut(s) 82, 316, 376
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 4 cut(s) 344, 351, 358, 384
PkrI GCNGC 3 cut(s) 158, 330, 333
PleI GAGTC 2 cut(s) 331, 399
PpsI GAGTC 2 cut(s) 331, 399
Psp124BI GAGCTC 1 cut(s) 110
PspEI GGTNACC 2 cut(s) 316, 376
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 3 cut(s) 157, 329, 332
SchI GAGTC 2 cut(s) 331, 400
ScrFI CCNGG 3 cut(s) 95, 236, 237
SduI GDGCHC 2 cut(s) 110, 273
SetI ASST 8 cut(s) 69, 82, 105, 110, 131, 201, 216, 304
SfaNI GCATC 2 cut(s) 17, 39
SmaI CCCGGG 1 cut(s) 237
SrfI GCCCGGGC 1 cut(s) 237
Sse9I AATT 3 cut(s) 21, 221, 397
SsiI CCGC 1 cut(s) 332
SspMI CTAG 1 cut(s) 255
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 93, 234, 235
TaaI ACNGT 1 cut(s) 37
TasI AATT 3 cut(s) 21, 221, 397
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 4 cut(s) 344, 351, 358, 384
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 2 cut(s) 40, 328
TseFI GTSAC 3 cut(s) 82, 316, 376
TseI GCWGC 2 cut(s) 156, 328
Tsp45I GTSAC 3 cut(s) 82, 316, 376
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 235
TspRI CASTG 2 cut(s) 40, 328
XapI RAATTY 1 cut(s) 397
XmaI CCCGGG 1 cut(s) 235
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.