Rmu_sc0000853.1_g000001

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000853.1
OG0003425
Physical Location & Seq
Forward (+)
1 .. 983
983 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 871 bp
gaaacgttcaagtgtactctgtccccaatgtcgagcagttgtgcaatttgttggaagaaaccacttcctgcataacattgctgatgaaatactgaatgcggattcttcactgagacgctccaacgaagacactgcagttttagattcctatgcaacagtacgatcaaatctggtaataaggtcgggaaaagggctgcttgggaaaagggttcgttcagcagtgcaagatgaagctgatagtgtagagcttccctgccctcaatgcagtaatcaatttggtcattttgcggtgcatattggcatgctcaaggtgttgcacgaagtgcttctcaccacatatgcagtcgcgatactttcaaaccaatctcagaacgcaccatctctagaattcctacttcagcatggtgttgcacaaagtgcttctcaccacatatgcagtcgcgatactttcaaaccaatctcagaacgcaccatctctagaattcctacttcagcacatgaaaacaatcgccatgaacaagaaatcactatgaggtgtattgcacaaatgggaagaacattgcaggatgttattgccgaatggatggtaatgttcaataacagaacaattgatcgaactaacaggatgccactgaagcatgccgagctgataacttctgggacatatacatgcagcgattgttatgagaaactgatctcattcctcttgtactggtttcgggtttcagtttctgcatcccatcttcccccagatgcatcaaaaagggaaaattgctggtatggatattcatgtcgaacacagcaccataatgaagaccatgctcgtaaaaggaatcatgtatgccggccgactaggggtgctaatgtgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

289

Amino Acids

32.78

Weight (kDa)

8.9

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CRD PF17979 144 - 242 6.9e-24 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 348, 442
AciI CCGC 2 cut(s) 99, 288
AclI AACGTT 1 cut(s) 5
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 2 cut(s) 387, 481
AcuI CTGAAG 3 cut(s) 381, 475, 654
AdeI CACNNNGTG 2 cut(s) 323, 417
AfaI GTAC 3 cut(s) 16, 160, 711
AfiI CCNNNNNNNGG 1 cut(s) 855
AgsI TTSAA 4 cut(s) 10, 358, 452, 596
AluBI AGCT 3 cut(s) 234, 248, 647
AluI AGCT 3 cut(s) 234, 248, 647
Alw26I GTCTC 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 732
AoxI GGCC 1 cut(s) 846
ApeKI GCWGC 2 cut(s) 194, 673
ApoI RAATTY 2 cut(s) 387, 481
Asp700I GAANNNNTTC 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 323, 417
BbsI GAAGAC 2 cut(s) 133, 820
BbvI GCAGC 2 cut(s) 181, 685
BccI CCATC 4 cut(s) 386, 480, 578, 748
BcgI CGANNNNNNTGC 2 cut(s) 114, 148
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 3 cut(s) 384, 478, 853
BfmI CTRYAG 1 cut(s) 133
BisI GCNGC 2 cut(s) 195, 674
BlsI GCNGC 2 cut(s) 196, 675
BmsI GCATC 4 cut(s) 616, 743, 744, 765
BpiI GAAGAC 2 cut(s) 133, 820
BpuEI CTTGAG 1 cut(s) 291
Bsc4I CCNNNNNNNGG 1 cut(s) 855
Bse118I RCCGGY 1 cut(s) 844
Bse1I ACTGG 1 cut(s) 717
Bse3DI GCAATG 2 cut(s) 76, 558
BseGI GGATG 4 cut(s) 572, 589, 631, 735
BseLI CCNNNNNNNGG 1 cut(s) 855
BseMI GCAATG 2 cut(s) 76, 558
BseMII CTCAG 3 cut(s) 102, 381, 475
BseNI ACTGG 1 cut(s) 717
BseX3I CGGCCG 1 cut(s) 846
BseXI GCAGC 2 cut(s) 181, 685
Bsh1236I CGCG 2 cut(s) 348, 442
Bsh1285I CGRYCG 1 cut(s) 849
BshFI GGCC 1 cut(s) 848
BsiEI CGRYCG 1 cut(s) 849
BsiSI CCGG 1 cut(s) 845
BslFI GGGAC 2 cut(s) 7, 674
BslI CCNNNNNNNGG 1 cut(s) 855
BsmAI GTCTC 1 cut(s) 107
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 2 cut(s) 7, 674
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 1 cut(s) 848
Bsp143I GATC 3 cut(s) 162, 611, 694
Bsp68I TCGCGA 2 cut(s) 348, 442
BspACI CCGC 2 cut(s) 99, 288
BspANI GGCC 1 cut(s) 848
BspCNI CTCAG 3 cut(s) 103, 380, 474
BspFNI CGCG 2 cut(s) 348, 442
BspMAI CTGCAG 1 cut(s) 137
BsrDI GCAATG 2 cut(s) 76, 558
BsrFI RCCGGY 1 cut(s) 844
BsrI ACTGG 1 cut(s) 717
BssAI RCCGGY 1 cut(s) 844
BssMI GATC 3 cut(s) 162, 611, 694
Bst4CI ACNGT 1 cut(s) 158
BstAPI GCANNNNNTGC 2 cut(s) 323, 417
BstC8I GCNNGC 3 cut(s) 303, 640, 846
BstDEI CTNAG 3 cut(s) 111, 367, 461
BstF5I GGATG 4 cut(s) 572, 589, 631, 735
BstFNI CGCG 2 cut(s) 348, 442
BstKTI GATC 3 cut(s) 165, 614, 697
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 3 cut(s) 162, 611, 694
BstMCI CGRYCG 1 cut(s) 849
BstMWI GCNNNNNNNGC 5 cut(s) 262, 323, 417, 635, 644
BstNSI RCATGY 3 cut(s) 305, 642, 673
BstSFI CTRYAG 1 cut(s) 133
BstUI CGCG 2 cut(s) 348, 442
BstV1I GCAGC 2 cut(s) 181, 685
BstV2I GAAGAC 2 cut(s) 133, 820
BstZI CGGCCG 1 cut(s) 846
BsuRI GGCC 1 cut(s) 848
BtsCI GGATG 4 cut(s) 572, 589, 631, 735
BtsI GCAGTG 2 cut(s) 130, 226
BtsIMutI CAGTG 4 cut(s) 108, 130, 226, 630
BtuMI TCGCGA 2 cut(s) 348, 442
Cac8I GCNNGC 3 cut(s) 303, 640, 846
CaiI CAGNNNCTG 1 cut(s) 732
Cfr10I RCCGGY 1 cut(s) 844
CseI GACGC 1 cut(s) 124
Csp6I GTAC 3 cut(s) 15, 159, 710
CviAII CATG 9 cut(s) 302, 402, 498, 513, 639, 670, 790, 819, 837
CviJI RGCY 5 cut(s) 194, 234, 248, 647, 848
CviKI_1 RGCY 5 cut(s) 194, 234, 248, 647, 848
CviQI GTAC 3 cut(s) 15, 159, 710
DdeI CTNAG 3 cut(s) 111, 367, 461
DpnI GATC 3 cut(s) 164, 613, 696
DpnII GATC 3 cut(s) 162, 611, 694
DraIII CACNNNGTG 2 cut(s) 323, 417
EaeI YGGCCR 1 cut(s) 846
EagI CGGCCG 1 cut(s) 846
EclXI CGGCCG 1 cut(s) 846
Eco52I CGGCCG 1 cut(s) 846
Eco57I CTGAAG 3 cut(s) 381, 475, 654
EcoRI GAATTC 2 cut(s) 387, 481
EcoT22I ATGCAT 1 cut(s) 758
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 9 cut(s) 305, 405, 501, 516, 642, 673, 793, 822, 840
FalI AAGNNNNNCTT 2 cut(s) 181, 213
FaqI GGGAC 2 cut(s) 7, 674
FatI CATG 9 cut(s) 301, 401, 497, 512, 638, 669, 789, 818, 836
FauNDI CATATG 2 cut(s) 338, 432
Fnu4HI GCNGC 2 cut(s) 195, 674
FokI GGATG 4 cut(s) 579, 596, 638, 722
Fsp4HI GCNGC 2 cut(s) 195, 674
FspBI CTAG 3 cut(s) 384, 478, 853
GluI GCNGC 2 cut(s) 195, 674
HaeIII GGCC 1 cut(s) 848
HapII CCGG 1 cut(s) 845
HgaI GACGC 1 cut(s) 124
Hin1II CATG 9 cut(s) 305, 405, 501, 516, 642, 673, 793, 822, 840
HinfI GANTC 3 cut(s) 102, 144, 833
HpaII CCGG 1 cut(s) 845
HphI GGTGA 2 cut(s) 323, 417
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 2 cut(s) 370, 464
Hpy188III TCNNGA 5 cut(s) 184, 347, 384, 441, 478
Hpy8I GTNNAC 1 cut(s) 15
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 1 cut(s) 5
HpyF10VI GCNNNNNNNGC 5 cut(s) 262, 323, 417, 635, 644
HpyF3I CTNAG 3 cut(s) 111, 367, 461
HpySE526I ACGT 1 cut(s) 5
Hsp92II CATG 9 cut(s) 305, 405, 501, 516, 642, 673, 793, 822, 840
KroI GCCGGC 1 cut(s) 844
KroNI GCCGGC 1 cut(s) 846
Kzo9I GATC 3 cut(s) 162, 611, 694
LmnI GCTCC 1 cut(s) 123
Lsp1109I GCAGC 2 cut(s) 181, 685
LweI GCATC 4 cut(s) 616, 743, 744, 765
MaeI CTAG 3 cut(s) 384, 478, 853
MaeII ACGT 1 cut(s) 5
MalI GATC 3 cut(s) 164, 613, 696
MboI GATC 3 cut(s) 162, 611, 694
MboII GAAGA 6 cut(s) 67, 97, 138, 565, 735, 825
MfeI CAATTG 1 cut(s) 607
MluCI AATT 6 cut(s) 45, 273, 387, 481, 607, 770
MmeI TCCRAC 2 cut(s) 32, 145
MnlI CCTC 3 cut(s) 268, 526, 714
Mph1103I ATGCAT 1 cut(s) 758
MroNI GCCGGC 1 cut(s) 844
MroXI GAANNNNTTC 1 cut(s) 325
MslI CAYNNNNRTG 2 cut(s) 668, 808
MspI CCGG 1 cut(s) 845
MunI CAATTG 1 cut(s) 607
Mva1269I GAATGC 1 cut(s) 101
MvnI CGCG 2 cut(s) 348, 442
MwoI GCNNNNNNNGC 5 cut(s) 262, 323, 417, 635, 644
NaeI GCCGGC 1 cut(s) 846
NdeI CATATG 2 cut(s) 338, 432
NdeII GATC 3 cut(s) 162, 611, 694
NgoMIV GCCGGC 1 cut(s) 844
NlaIII CATG 9 cut(s) 305, 405, 501, 516, 642, 673, 793, 822, 840
NmeAIII GCCGAG 1 cut(s) 668
NruI TCGCGA 2 cut(s) 348, 442
NsiI ATGCAT 1 cut(s) 758
NspI RCATGY 3 cut(s) 305, 642, 673
PaeI GCATGC 2 cut(s) 305, 642
PctI GAATGC 1 cut(s) 101
PdiI GCCGGC 1 cut(s) 846
PdmI GAANNNNTTC 1 cut(s) 325
PfeI GAWTC 3 cut(s) 102, 144, 833
PkrI GCNGC 2 cut(s) 196, 675
Psp1406I AACGTT 1 cut(s) 5
PstI CTGCAG 1 cut(s) 137
PstNI CAGNNNCTG 1 cut(s) 732
RruI TCGCGA 2 cut(s) 348, 442
RsaI GTAC 3 cut(s) 16, 160, 711
RsaNI GTAC 3 cut(s) 15, 159, 710
RseI CAYNNNNRTG 2 cut(s) 668, 808
SatI GCNGC 2 cut(s) 195, 674
Sau3AI GATC 3 cut(s) 162, 611, 694
SetI ASST 7 cut(s) 8, 183, 236, 250, 313, 537, 649
SfaNI GCATC 4 cut(s) 616, 743, 744, 765
SfcI CTRYAG 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 668, 808
SmlI CTYRAG 1 cut(s) 306
SmoI CTYRAG 1 cut(s) 306
SphI GCATGC 2 cut(s) 305, 642
Sse9I AATT 6 cut(s) 45, 273, 387, 481, 607, 770
SsiI CCGC 2 cut(s) 99, 288
SspMI CTAG 3 cut(s) 384, 478, 853
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 1 cut(s) 8
TaqI TCGA 3 cut(s) 32, 614, 794
TasI AATT 6 cut(s) 45, 273, 387, 481, 607, 770
TatI WGTACW 2 cut(s) 14, 709
TfiI GAWTC 3 cut(s) 102, 144, 833
TscAI CASTG 4 cut(s) 115, 137, 226, 637
TseI GCWGC 2 cut(s) 194, 673
TspDTI ATGAA 6 cut(s) 100, 244, 514, 529, 778, 826
TspRI CASTG 4 cut(s) 115, 137, 226, 637
XapI RAATTY 2 cut(s) 387, 481
XbaI TCTAGA 2 cut(s) 383, 477
XceI RCATGY 3 cut(s) 305, 642, 673
XmnI GAANNNNTTC 1 cut(s) 325
XspI CTAG 3 cut(s) 384, 478, 853
Zsp2I ATGCAT 1 cut(s) 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.