Rmu_sc0001460.1_g000050

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001460.1
OG0003166
Physical Location & Seq
Forward (+)
213614 .. 217157
3544 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1269 bp
atgctcctacatcagtcgctggattccattagcattgtaatttggaagcctactatactgcctaagttgctgtttcatactttacagggttttgggatgaaaatcagtaaaacagaggtgaatttgaggaggctgcttgcagctgctcctcagcaacaaaaccaggcaaaactttcgcattatgttgctactttacgagaacaggtagagcagctagctgaagagagaacaccagaagggttacctagagtttcaaaggctgttttgagtgactattcagagaagatcgaagccatagctttgaaattatctgctccgttgccaaatttgcaagaaccccaggagtccctggcaagaatttcggttaaagcaaagtcttctgaaacaggagacaatcagagtccccattctccaggtctgagaagaagatttgtgcccaactcaaacatccaagatggaactcgtgagactcttagtgcagattcttcggcacctgtcaaacttgatgctgcagctcaagcacacattgaaaagcataggagacttcaggaggatttgactgatgaaatggttgggttggcaaggcaacttaaagagactagtctcatgatgagccactccgtacaaaacactgaaaaactacttgactctacagaggaggctgtagaaaggagcttggcaagcactggtcatgccactgcacgggcatccgatatatactcaaagacctccaagacttcagtatccttaggtaggaagacggacaggtttcacatggcttttgatgtttctaatggcatgtatattcatcatggaggtcggcttcgtcttctccagatcagtttcctcttctttttttgctgttgcacagaaattaggagagtctttgggaagtgtaaagtgagcagattcgtcaatcaaaggtttcagtcgccttcatctacatcggacccgattcccggcttcaccatcgatttccagattgcccagaaccggcaatcttcgctcgctcagctccgtcgtcggtcttcaaatcgctcaggaccatcgctgtcgatttccagatcgcccagaaccggcgatcgtcgctcgtccagctccgtcgcccaacaaccagggatcgacgctcacccaggacaggcgatcctctctcctcgctcagccgtcagttccctaaaccagaccggcctagtctcttgtcagccgccttcgtctgcctcttacatcagagatcgatctccaatctcttcagttggtcaactcggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Use1

Protein Analysis

422

Amino Acids

46.49

Weight (kDa)

9.88

Isoelectric Point (pI)

72.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Use1 PF09753 37 - 247 9.8e-51 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 490
AciI CCGC 1 cut(s) 1205
AclWI GGATC 2 cut(s) 1127, 1138
AcsI RAATTY 3 cut(s) 121, 325, 357
AcuI CTGAAG 4 cut(s) 240, 530, 723, 1233
AfaI GTAC 1 cut(s) 624
AfiI CCNNNNNNNGG 6 cut(s) 702, 753, 959, 993, 1076, 1138
AgsI TTSAA 4 cut(s) 255, 304, 530, 1032
AhlI ACTAGT 1 cut(s) 599
AjnI CCWGG 6 cut(s) 162, 339, 348, 412, 1114, 1132
AluBI AGCT 8 cut(s) 143, 214, 218, 299, 515, 675, 1015, 1098
AluI AGCT 8 cut(s) 143, 214, 218, 299, 515, 675, 1015, 1098
Alw26I GTCTC 6 cut(s) 384, 461, 535, 590, 608, 1198
AlwI GGATC 2 cut(s) 1127, 1138
AlwNI CAGNNNCTG 1 cut(s) 19
AoxI GGCC 1 cut(s) 1186
ApeKI GCWGC 6 cut(s) 133, 140, 143, 211, 509, 512
ApoI RAATTY 3 cut(s) 121, 325, 357
AspS9I GGNCC 2 cut(s) 949, 1043
AsuC2I CCSGG 1 cut(s) 960
AsuHPI GGTGA 3 cut(s) 130, 958, 1121
AsuNHI GCTAGC 1 cut(s) 214
AvaII GGWCC 2 cut(s) 949, 1043
AxyI CCTNAGG 1 cut(s) 748
BaeGI GKGCMC 1 cut(s) 438
BaeI ACNNNNGTAYC 2 cut(s) 726, 759
BanI GGYRCC 1 cut(s) 490
BauI CACGAG 1 cut(s) 462
BbsI GAAGAC 4 cut(s) 369, 764, 821, 1020
BbvCI CCTCAGC 1 cut(s) 150
BbvI GCAGC 6 cut(s) 120, 130, 152, 223, 496, 524
BccI CCATC 3 cut(s) 449, 977, 1054
BceAI ACGGC 1 cut(s) 1148
BcgI CGANNNNNNTGC 6 cut(s) 156, 190, 342, 376, 468, 502
BciT130I CCWGG 6 cut(s) 164, 341, 350, 414, 1116, 1134
BciVI GTATCC 1 cut(s) 754
BcnI CCSGG 1 cut(s) 960
BcoDI GTCTC 6 cut(s) 384, 461, 535, 590, 608, 1198
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 4 cut(s) 215, 246, 600, 1190
BfmI CTRYAG 3 cut(s) 510, 651, 663
BfuI GTATCC 1 cut(s) 754
BisI GCNGC 7 cut(s) 134, 141, 144, 212, 510, 513, 1205
BlpI GCTNAGC 2 cut(s) 1011, 1159
BlsI GCNGC 7 cut(s) 135, 142, 145, 213, 511, 514, 1206
Bme1390I CCNGG 7 cut(s) 164, 341, 350, 414, 960, 1116, 1134
Bme18I GGWCC 2 cut(s) 949, 1043
BmgT120I GGNCC 2 cut(s) 949, 1043
BmiI GGNNCC 2 cut(s) 492, 951
BmrFI CCNGG 7 cut(s) 164, 341, 350, 414, 960, 1116, 1134
BmsI GCATC 2 cut(s) 496, 716
BmtI GCTAGC 1 cut(s) 218
BpiI GAAGAC 4 cut(s) 369, 764, 821, 1020
BpmI CTGGAG 2 cut(s) 396, 818
Bpu10I CCTNAGC 2 cut(s) 150, 1039
Bpu1102I GCTNAGC 2 cut(s) 1011, 1159
BpuEI CTTGAG 1 cut(s) 501
BpuMI CCSGG 1 cut(s) 960
Bsa29I ATCGAT 2 cut(s) 972, 1234
BsaBI GATNNNNATC 1 cut(s) 101
BsaJI CCNNGG 4 cut(s) 339, 348, 1115, 1132
Bsc4I CCNNNNNNNGG 6 cut(s) 702, 753, 959, 993, 1076, 1138
Bse118I RCCGGY 3 cut(s) 993, 1076, 1184
Bse1I ACTGG 1 cut(s) 691
Bse21I CCTNAGG 1 cut(s) 748
Bse8I GATNNNNATC 1 cut(s) 101
BseBI CCWGG 6 cut(s) 164, 341, 350, 414, 1116, 1134
BseCI ATCGAT 2 cut(s) 972, 1234
BseDI CCNNGG 4 cut(s) 339, 348, 1115, 1132
BseGI GGATG 3 cut(s) 102, 447, 707
BseJI GATNNNNATC 1 cut(s) 101
BseLI CCNNNNNNNGG 6 cut(s) 702, 753, 959, 993, 1076, 1138
BseMII CTCAG 5 cut(s) 164, 410, 1025, 1053, 1173
BseNI ACTGG 1 cut(s) 691
BseRI GAGGAG 4 cut(s) 138, 142, 671, 1143
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 6 cut(s) 120, 130, 152, 223, 496, 524
BsgI GTGCAG 2 cut(s) 498, 684
Bsh1285I CGRYCG 1 cut(s) 1084
BshFI GGCC 1 cut(s) 1188
BshNI GGYRCC 1 cut(s) 490
BshVI ATCGAT 2 cut(s) 972, 1234
BsiEI CGRYCG 1 cut(s) 1084
BsiSI CCGG 4 cut(s) 960, 994, 1077, 1185
BslFI GGGAC 2 cut(s) 331, 387
BslI CCNNNNNNNGG 6 cut(s) 702, 753, 959, 993, 1076, 1138
BsmAI GTCTC 6 cut(s) 384, 461, 535, 590, 608, 1198
BsmFI GGGAC 2 cut(s) 331, 387
BsnI GGCC 1 cut(s) 1188
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 8 cut(s) 285, 837, 1064, 1081, 1119, 1143, 1231, 1235
Bsp1720I GCTNAGC 2 cut(s) 1011, 1159
BspACI CCGC 1 cut(s) 1205
BspANI GGCC 1 cut(s) 1188
BspCNI CTCAG 5 cut(s) 163, 411, 1024, 1052, 1172
BspDI ATCGAT 2 cut(s) 972, 1234
BspHI TCATGA 1 cut(s) 606
BspLI GGNNCC 2 cut(s) 492, 951
BspMAI CTGCAG 1 cut(s) 514
BspOI GCTAGC 1 cut(s) 218
BspPI GGATC 2 cut(s) 1127, 1138
BspT107I GGYRCC 1 cut(s) 490
BsrFI RCCGGY 3 cut(s) 993, 1076, 1184
BsrI ACTGG 1 cut(s) 691
BssAI RCCGGY 3 cut(s) 993, 1076, 1184
BssECI CCNNGG 4 cut(s) 339, 348, 1115, 1132
BssMI GATC 8 cut(s) 285, 837, 1064, 1081, 1119, 1143, 1231, 1235
BssSI CACGAG 1 cut(s) 462
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 6 cut(s) 164, 341, 350, 414, 1116, 1134
Bst6I CTCTTC 3 cut(s) 216, 854, 1252
BstC8I GCNNGC 4 cut(s) 138, 216, 682, 1008
BstDEI CTNAG 8 cut(s) 63, 150, 419, 473, 748, 1011, 1039, 1159
BstEII GGTNACC 1 cut(s) 240
BstENI CCTNNNNNAGG 1 cut(s) 751
BstF5I GGATG 3 cut(s) 102, 447, 707
BstKTI GATC 8 cut(s) 288, 840, 1067, 1084, 1122, 1146, 1234, 1238
BstMAI GTCTC 6 cut(s) 384, 461, 535, 590, 608, 1198
BstMBI GATC 8 cut(s) 285, 837, 1064, 1081, 1119, 1143, 1231, 1235
BstMCI CGRYCG 1 cut(s) 1084
BstMWI GCNNNNNNNGC 8 cut(s) 67, 328, 518, 681, 1003, 1012, 1086, 1095
BstNI CCWGG 6 cut(s) 164, 341, 350, 414, 1116, 1134
BstNSI RCATGY 1 cut(s) 802
BstPI GGTNACC 1 cut(s) 240
BstSCI CCNGG 7 cut(s) 162, 339, 348, 412, 958, 1114, 1132
BstSFI CTRYAG 3 cut(s) 510, 651, 663
BstSLI GKGCMC 1 cut(s) 438
BstV1I GCAGC 6 cut(s) 120, 130, 152, 223, 496, 524
BstV2I GAAGAC 4 cut(s) 369, 764, 821, 1020
Bsu15I ATCGAT 2 cut(s) 972, 1234
Bsu36I CCTNAGG 1 cut(s) 748
BsuI GTATCC 1 cut(s) 754
BsuRI GGCC 1 cut(s) 1188
BsuTUI ATCGAT 2 cut(s) 972, 1234
BtgZI GCGATG 1 cut(s) 1032
BtsCI GGATG 3 cut(s) 102, 447, 707
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 3 cut(s) 630, 684, 696
Cac8I GCNNGC 4 cut(s) 138, 216, 682, 1008
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 606
Cfr10I RCCGGY 3 cut(s) 993, 1076, 1184
Cfr13I GGNCC 2 cut(s) 949, 1043
ClaI ATCGAT 2 cut(s) 972, 1234
CseI GACGC 1 cut(s) 1133
Csp6I GTAC 1 cut(s) 623
CviAII CATG 5 cut(s) 607, 692, 775, 799, 812
CviQI GTAC 1 cut(s) 623
DdeI CTNAG 8 cut(s) 63, 150, 419, 473, 748, 1011, 1039, 1159
DpnI GATC 8 cut(s) 287, 839, 1066, 1083, 1121, 1145, 1233, 1237
DpnII GATC 8 cut(s) 285, 837, 1064, 1081, 1119, 1143, 1231, 1235
Eam1104I CTCTTC 3 cut(s) 216, 854, 1252
EarI CTCTTC 3 cut(s) 216, 854, 1252
Eco47I GGWCC 2 cut(s) 949, 1043
Eco57I CTGAAG 4 cut(s) 240, 530, 723, 1233
Eco81I CCTNAGG 1 cut(s) 748
Eco91I GGTNACC 1 cut(s) 240
EcoNI CCTNNNNNAGG 1 cut(s) 751
EcoO65I GGTNACC 1 cut(s) 240
EcoRII CCWGG 6 cut(s) 162, 339, 348, 412, 1114, 1132
FaeI CATG 5 cut(s) 610, 695, 778, 802, 815
FaqI GGGAC 2 cut(s) 331, 387
FatI CATG 5 cut(s) 606, 691, 774, 798, 811
Fnu4HI GCNGC 7 cut(s) 134, 141, 144, 212, 510, 513, 1205
FokI GGATG 3 cut(s) 109, 434, 694
Fsp4HI GCNGC 7 cut(s) 134, 141, 144, 212, 510, 513, 1205
FspBI CTAG 4 cut(s) 215, 246, 600, 1190
GluI GCNGC 7 cut(s) 134, 141, 144, 212, 510, 513, 1205
GsuI CTGGAG 2 cut(s) 396, 818
HaeIII GGCC 1 cut(s) 1188
HapII CCGG 4 cut(s) 960, 994, 1077, 1185
HgaI GACGC 1 cut(s) 1133
Hin1II CATG 5 cut(s) 610, 695, 778, 802, 815
HincII GTYRAC 1 cut(s) 1259
HindII GTYRAC 1 cut(s) 1259
HinfI GANTC 9 cut(s) 23, 344, 400, 469, 482, 647, 882, 909, 955
HpaII CCGG 4 cut(s) 960, 994, 1077, 1185
HphI GGTGA 3 cut(s) 130, 958, 1121
Hpy166II GTNNAC 1 cut(s) 1259
Hpy188I TCNGA 7 cut(s) 280, 382, 399, 420, 712, 949, 1229
Hpy188III TCNNGA 7 cut(s) 464, 548, 607, 835, 979, 1041, 1062
Hpy8I GTNNAC 1 cut(s) 1259
Hpy99I CGWCG 5 cut(s) 1023, 1026, 1089, 1106, 1127
HpyAV CCTTC 3 cut(s) 230, 945, 1218
HpyCH4V TGCA 6 cut(s) 140, 331, 479, 512, 701, 867
HpyF10VI GCNNNNNNNGC 8 cut(s) 67, 328, 518, 681, 1003, 1012, 1086, 1095
HpyF3I CTNAG 8 cut(s) 63, 150, 419, 473, 748, 1011, 1039, 1159
Hsp92II CATG 5 cut(s) 610, 695, 778, 802, 815
Kzo9I GATC 8 cut(s) 285, 837, 1064, 1081, 1119, 1143, 1231, 1235
LmnI GCTCC 6 cut(s) 9, 151, 319, 672, 1020, 1103
Lsp1109I GCAGC 6 cut(s) 120, 130, 152, 223, 496, 524
LweI GCATC 2 cut(s) 496, 716
MaeI CTAG 4 cut(s) 215, 246, 600, 1190
MaeIII GTNAC 2 cut(s) 240, 269
MalI GATC 8 cut(s) 287, 839, 1066, 1083, 1121, 1145, 1233, 1237
MboI GATC 8 cut(s) 285, 837, 1064, 1081, 1119, 1143, 1231, 1235
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 6 cut(s) 39, 121, 305, 325, 357, 873
MlyI GAGTC 5 cut(s) 353, 409, 463, 641, 891
MseI TTAA 3 cut(s) 366, 591, 1267
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 4 cut(s) 960, 994, 1077, 1185
MspR9I CCNGG 7 cut(s) 164, 341, 350, 414, 960, 1116, 1134
MvaI CCWGG 6 cut(s) 164, 341, 350, 414, 1116, 1134
MwoI GCNNNNNNNGC 8 cut(s) 67, 328, 518, 681, 1003, 1012, 1086, 1095
NciI CCSGG 1 cut(s) 960
NdeII GATC 8 cut(s) 285, 837, 1064, 1081, 1119, 1143, 1231, 1235
NheI GCTAGC 1 cut(s) 214
NlaIII CATG 5 cut(s) 610, 695, 778, 802, 815
NlaIV GGNNCC 2 cut(s) 492, 951
NmuCI GTSAC 1 cut(s) 269
NspI RCATGY 1 cut(s) 802
PagI TCATGA 1 cut(s) 606
PfeI GAWTC 4 cut(s) 23, 482, 909, 955
PkrI GCNGC 7 cut(s) 135, 142, 145, 213, 511, 514, 1206
Ple19I CGATCG 1 cut(s) 1084
PleI GAGTC 5 cut(s) 352, 408, 463, 641, 890
PpsI GAGTC 5 cut(s) 352, 408, 463, 641, 890
Psp6I CCWGG 6 cut(s) 162, 339, 348, 412, 1114, 1132
PspEI GGTNACC 1 cut(s) 240
PspGI CCWGG 6 cut(s) 162, 339, 348, 412, 1114, 1132
PspN4I GGNNCC 2 cut(s) 492, 951
PspPI GGNCC 2 cut(s) 949, 1043
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 19
PvuI CGATCG 1 cut(s) 1084
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 1 cut(s) 624
RsaNI GTAC 1 cut(s) 623
SaqAI TTAA 3 cut(s) 366, 591, 1267
SatI GCNGC 7 cut(s) 134, 141, 144, 212, 510, 513, 1205
Sau3AI GATC 8 cut(s) 285, 837, 1064, 1081, 1119, 1143, 1231, 1235
Sau96I GGNCC 2 cut(s) 949, 1043
SchI GAGTC 5 cut(s) 353, 409, 463, 641, 891
ScrFI CCNGG 7 cut(s) 164, 341, 350, 414, 960, 1116, 1134
SduI GDGCHC 1 cut(s) 438
SfaNI GCATC 2 cut(s) 496, 716
SfcI CTRYAG 3 cut(s) 510, 651, 663
SinI GGWCC 2 cut(s) 949, 1043
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 6 cut(s) 39, 121, 305, 325, 357, 873
SsiI CCGC 1 cut(s) 1205
SspMI CTAG 4 cut(s) 215, 246, 600, 1190
StyD4I CCNGG 7 cut(s) 162, 339, 348, 412, 958, 1114, 1132
TaqI TCGA 5 cut(s) 288, 972, 1055, 1122, 1234
TaqII GACCGA 1 cut(s) 1014
TasI AATT 6 cut(s) 39, 121, 305, 325, 357, 873
TauI GCSGC 1 cut(s) 1207
TfiI GAWTC 4 cut(s) 23, 482, 909, 955
Tru1I TTAA 3 cut(s) 366, 591, 1267
Tru9I TTAA 3 cut(s) 366, 591, 1267
TscAI CASTG 3 cut(s) 637, 691, 703
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 6 cut(s) 133, 140, 143, 211, 509, 512
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 5 cut(s) 65, 113, 579, 797, 927
TspGWI ACGGA 5 cut(s) 306, 610, 776, 1007, 1090
TspRI CASTG 3 cut(s) 637, 691, 703
VpaK11BI GGWCC 2 cut(s) 949, 1043
XagI CCTNNNNNAGG 1 cut(s) 751
XapI RAATTY 3 cut(s) 121, 325, 357
XceI RCATGY 1 cut(s) 802
XspI CTAG 4 cut(s) 215, 246, 600, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.