Rmu_sc0001659.1_g000041

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001659.1
OG0010609
Physical Location & Seq
Forward (+)
156337 .. 157405
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 762 bp
atgaaagtactaaaggggtatgtctgcaatcgttttcatcctgagggttgcatagctaagagctaccttggagaagaatctgttgagttttgctgcgagtttttacaacaatgtagttcagttggtgttcctaagggtccatctaaggtctctggcccactttccggtgcgaagctgaagtcaatagatgaagaagtgagagaccaggcacatctccatgtgttgttcaataatgctgaggtggatccatatagaaagacacataagaagattgtggagcagcaatacggaggaaaaaagaagagcaaaaagtggctacaaagtgagcacaatcgcacctttgccgattggtttcaatccaaggtgtcagctgaacttaagtgtagaccaaacaatgtctctcatatagtgaagtggctggctggaaaaccaagttttacagtttttacttatgaagcctatcgatataatggagctaaatactttacaaagcagagagacaatgctagagctgtccaaaatagccacagaaacgataaatttgctactgccggtcaagtgaaacaagttttctacgttgaagatccctttgatgctgactggtctgttgtgctaacgaccccgaacagagattatcatgattctttccatgacgatgatcttggggacactatcatggaagtacaaccattctgtgtagaaattccatattgtgaggaagatgagaatgaggatgattccacttacaagagcgaatgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

253

Amino Acids

29.09

Weight (kDa)

6.15

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4218 PF13960 2 - 40 2.7e-07 Domain of unknown function (DUF4218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 385
AclWI GGATC 3 cut(s) 239, 252, 578
AcsI RAATTY 2 cut(s) 539, 702
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 2 cut(s) 9, 684
AfiI CCNNNNNNNGG 1 cut(s) 164
AflII CTTAAG 1 cut(s) 377
AgsI TTSAA 3 cut(s) 229, 356, 581
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 6 cut(s) 56, 63, 175, 371, 476, 512
AluI AGCT 6 cut(s) 56, 63, 175, 371, 476, 512
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 4 cut(s) 154, 195, 403, 492
AlwI GGATC 3 cut(s) 239, 252, 578
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 2 cut(s) 93, 280
ApoI RAATTY 2 cut(s) 539, 702
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AspS9I GGNCC 2 cut(s) 137, 155
AvaII GGWCC 1 cut(s) 137
AxyI CCTNAGG 2 cut(s) 42, 132
BamHI GGATCC 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 80, 292
BccI CCATC 1 cut(s) 148
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 1 cut(s) 206
BcoDI GTCTC 4 cut(s) 154, 195, 403, 492
BfaI CTAG 1 cut(s) 507
BfrI CTTAAG 1 cut(s) 377
BisI GCNGC 2 cut(s) 94, 281
BlsI GCNGC 2 cut(s) 95, 282
BmcAI AGTACT 1 cut(s) 9
Bme1390I CCNGG 1 cut(s) 206
Bme18I GGWCC 1 cut(s) 137
BmgT120I GGNCC 2 cut(s) 137, 155
BmiI GGNNCC 2 cut(s) 138, 246
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 1 cut(s) 583
Bpu10I CCTNAGC 1 cut(s) 237
Bsa29I ATCGAT 1 cut(s) 463
BsaI GGTCTC 2 cut(s) 154, 195
BsaJI CCNNGG 2 cut(s) 67, 360
BsaWI WCCGGW 1 cut(s) 164
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse118I RCCGGY 1 cut(s) 551
Bse1I ACTGG 1 cut(s) 605
Bse21I CCTNAGG 2 cut(s) 42, 132
BseBI CCWGG 1 cut(s) 206
BseCI ATCGAT 1 cut(s) 463
BseDI CCNNGG 2 cut(s) 67, 360
BseGI GGATG 2 cut(s) 37, 739
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMII CTCAG 2 cut(s) 33, 228
BseNI ACTGG 1 cut(s) 605
BseXI GCAGC 2 cut(s) 80, 292
BshFI GGCC 1 cut(s) 156
BshVI ATCGAT 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 2 cut(s) 165, 552
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 4 cut(s) 154, 195, 403, 492
BsmFI GGGAC 1 cut(s) 680
BsnI GGCC 1 cut(s) 156
Bso31I GGTCTC 2 cut(s) 154, 195
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 3 cut(s) 244, 583, 658
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 2 cut(s) 34, 229
BspDI ATCGAT 1 cut(s) 463
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 2 cut(s) 138, 246
BspPI GGATC 3 cut(s) 239, 252, 578
BspQI GCTCTTC 1 cut(s) 296
BspTI CTTAAG 1 cut(s) 377
BspTNI GGTCTC 2 cut(s) 154, 195
BsrFI RCCGGY 1 cut(s) 551
BsrI ACTGG 1 cut(s) 605
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 2 cut(s) 67, 360
BssMI GATC 3 cut(s) 244, 583, 658
BssT1I CCWWGG 2 cut(s) 67, 360
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 296
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 5 cut(s) 42, 57, 132, 144, 237
BstF5I GGATG 2 cut(s) 37, 739
BstKTI GATC 3 cut(s) 247, 586, 661
BstMAI GTCTC 4 cut(s) 154, 195, 403, 492
BstMBI GATC 3 cut(s) 244, 583, 658
BstNI CCWGG 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 204
BstV1I GCAGC 2 cut(s) 80, 292
BstX2I RGATCY 2 cut(s) 244, 583
BstYI RGATCY 2 cut(s) 244, 583
Bsu15I ATCGAT 1 cut(s) 463
Bsu36I CCTNAGG 2 cut(s) 42, 132
BsuRI GGCC 1 cut(s) 156
BsuTUI ATCGAT 1 cut(s) 463
BtsCI GGATG 2 cut(s) 37, 739
Cac8I GCNNGC 1 cut(s) 420
CciI TCATGA 1 cut(s) 637
Cfr10I RCCGGY 1 cut(s) 551
Cfr13I GGNCC 2 cut(s) 137, 155
ClaI ATCGAT 1 cut(s) 463
Csp6I GTAC 2 cut(s) 8, 683
CviAII CATG 4 cut(s) 218, 638, 650, 676
CviQI GTAC 2 cut(s) 8, 683
DdeI CTNAG 5 cut(s) 42, 57, 132, 144, 237
DpnI GATC 3 cut(s) 246, 585, 660
DpnII GATC 3 cut(s) 244, 583, 658
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco130I CCWWGG 2 cut(s) 67, 360
Eco31I GGTCTC 2 cut(s) 154, 195
Eco47I GGWCC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 197
Eco81I CCTNAGG 2 cut(s) 42, 132
EcoRII CCWGG 1 cut(s) 204
EcoT14I CCWWGG 2 cut(s) 67, 360
ErhI CCWWGG 2 cut(s) 67, 360
FaeI CATG 4 cut(s) 221, 641, 653, 679
FaqI GGGAC 1 cut(s) 680
FatI CATG 4 cut(s) 217, 637, 649, 675
FblI GTMKAC 1 cut(s) 385
Fnu4HI GCNGC 2 cut(s) 94, 281
FokI GGATG 2 cut(s) 24, 746
Fsp4HI GCNGC 2 cut(s) 94, 281
FspBI CTAG 1 cut(s) 507
GluI GCNGC 2 cut(s) 94, 281
HaeIII GGCC 1 cut(s) 156
HapII CCGG 2 cut(s) 165, 552
Hin1II CATG 4 cut(s) 221, 641, 653, 679
HinfI GANTC 3 cut(s) 77, 641, 737
HpaII CCGG 2 cut(s) 165, 552
Hpy166II GTNNAC 1 cut(s) 386
Hpy188III TCNNGA 2 cut(s) 41, 638
Hpy8I GTNNAC 1 cut(s) 386
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 1 cut(s) 576
HpyCH4V TGCA 2 cut(s) 27, 51
HpyF3I CTNAG 5 cut(s) 42, 57, 132, 144, 237
HpySE526I ACGT 1 cut(s) 576
Hsp92II CATG 4 cut(s) 221, 641, 653, 679
Kzo9I GATC 3 cut(s) 244, 583, 658
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 2 cut(s) 277, 473
LpnPI CCDG 9 cut(s) 54, 138, 178, 191, 218, 404, 408, 565, 586
Lsp1109I GCAGC 2 cut(s) 80, 292
LweI GCATC 1 cut(s) 583
MaeI CTAG 1 cut(s) 507
MaeII ACGT 1 cut(s) 576
MalI GATC 3 cut(s) 246, 585, 660
MboI GATC 3 cut(s) 244, 583, 658
MboII GAAGA 6 cut(s) 86, 203, 280, 313, 593, 731
MflI RGATCY 2 cut(s) 244, 583
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 2 cut(s) 539, 702
MnlI CCTC 5 cut(s) 37, 232, 284, 709, 724
MseI TTAA 1 cut(s) 378
MslI CAYNNNNRTG 3 cut(s) 216, 654, 674
MspA1I CMGCKG 1 cut(s) 371
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 2 cut(s) 165, 552
MspR9I CCNGG 1 cut(s) 206
MvaI CCWGG 1 cut(s) 206
NdeII GATC 3 cut(s) 244, 583, 658
NlaIII CATG 4 cut(s) 221, 641, 653, 679
NlaIV GGNNCC 2 cut(s) 138, 246
PagI TCATGA 1 cut(s) 637
PciSI GCTCTTC 1 cut(s) 296
PfeI GAWTC 3 cut(s) 77, 641, 737
PkrI GCNGC 2 cut(s) 95, 282
Psp6I CCWGG 1 cut(s) 204
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 2 cut(s) 138, 246
PspPI GGNCC 2 cut(s) 137, 155
PsuI RGATCY 2 cut(s) 244, 583
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 2 cut(s) 9, 684
RsaNI GTAC 2 cut(s) 8, 683
RseI CAYNNNNRTG 3 cut(s) 216, 654, 674
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 2 cut(s) 94, 281
Sau3AI GATC 3 cut(s) 244, 583, 658
Sau96I GGNCC 2 cut(s) 137, 155
ScaI AGTACT 1 cut(s) 9
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 1 cut(s) 583
SinI GGWCC 1 cut(s) 137
SmiMI CAYNNNNRTG 3 cut(s) 216, 654, 674
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 2 cut(s) 539, 702
SspMI CTAG 1 cut(s) 507
StyD4I CCNGG 1 cut(s) 204
StyI CCWWGG 2 cut(s) 67, 360
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 1 cut(s) 579
TaqI TCGA 1 cut(s) 463
TasI AATT 2 cut(s) 539, 702
TatI WGTACW 2 cut(s) 7, 682
TfiI GAWTC 3 cut(s) 77, 641, 737
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TseI GCWGC 2 cut(s) 93, 280
TspDTI ATGAA 4 cut(s) 17, 26, 204, 468
TspGWI ACGGA 1 cut(s) 303
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 137
XapI RAATTY 2 cut(s) 539, 702
XmiI GTMKAC 1 cut(s) 385
XspI CTAG 1 cut(s) 507
ZrmI AGTACT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.