Rh5AG392900

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010609
Physical Location & Seq
Reverse (-)
68103484 .. 68118173
14690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG392900.1

Sequence Viewer

Length: 402 bp
ATGTTGATGATACCAAGTTGGTTGGAAGGAGCAGCAGCAATGGATAAATCTTGGATTAATGAAGATAGGGATACTTTGAAGTACCAATTAGGTGTGGAGAATTTTTTGATTTTTGCTGAAGAAAATGCTAGTAACCATAAAAGAATTCCTTGCCCTTGTTCAAGATGTGATTCAAGTAAGTTGTGTGGACCACTTTCTATTGCAAAACTAAAGTCCATAGATGAGAAAGAGAGAGACCAGGCACATCTTCATGTCCTATCCAATAATGCTGAAGTGGATCCGTATAAGAAGGCACATAGAAAGATTGTGGAGCAGTTGTACGGGGGAAAAATAAAGAGTAAACAATGGCTATTAAGTGAGCACAATCGTACTTTTGCAGATTGGTTTCAATCCAAGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

133

Amino Acids

15.27

Weight (kDa)

8.33

Isoelectric Point (pI)

23.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 16 - 61 6e-09 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 272, 285
AcsI RAATTY 2 cut(s) 100, 144
AcuI CTGAAG 2 cut(s) 138, 291
AfaI GTAC 3 cut(s) 83, 320, 370
AgsI TTSAA 4 cut(s) 79, 162, 174, 389
AjnI CCWGG 1 cut(s) 237
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 2 cut(s) 272, 285
ApeKI GCWGC 2 cut(s) 32, 35
ApoI RAATTY 2 cut(s) 100, 144
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AseI ATTAAT 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 188
AvaII GGWCC 1 cut(s) 188
BamHI GGATCC 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 44, 47
BciT130I CCWGG 1 cut(s) 239
BciVI GTATCC 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 1 cut(s) 129
BfuI GTATCC 1 cut(s) 64
BisI GCNGC 2 cut(s) 33, 36
BlsI GCNGC 2 cut(s) 34, 37
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 239
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 393
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 393
BseMI GCAATG 1 cut(s) 45
BseXI GCAGC 2 cut(s) 44, 47
BsiHKAI GWGCWC 1 cut(s) 363
BsmAI GTCTC 1 cut(s) 228
Bso31I GGTCTC 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 279
BspPI GGATC 2 cut(s) 272, 285
BspTNI GGTCTC 1 cut(s) 228
BsrDI GCAATG 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 393
BssMI GATC 1 cut(s) 277
BssT1I CCWWGG 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 239
BstKTI GATC 1 cut(s) 280
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 1 cut(s) 277
BstNI CCWGG 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 44, 47
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuI GTATCC 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 82, 319, 369
CviAII CATG 1 cut(s) 251
CviJI RGCY 1 cut(s) 349
CviKI_1 RGCY 1 cut(s) 349
CviQI GTAC 3 cut(s) 82, 319, 369
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 393
Eco31I GGTCTC 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 2 cut(s) 138, 291
EcoRI GAATTC 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 1 cut(s) 254
FaiI YATR 5 cut(s) 138, 218, 252, 285, 297
FalI AAGNNNNNCTT 2 cut(s) 133, 165
FatI CATG 1 cut(s) 250
Fnu4HI GCNGC 2 cut(s) 33, 36
Fsp4HI GCNGC 2 cut(s) 33, 36
FspBI CTAG 1 cut(s) 129
GluI GCNGC 2 cut(s) 33, 36
Hin1II CATG 1 cut(s) 254
HinfI GANTC 1 cut(s) 170
Hpy166II GTNNAC 2 cut(s) 188, 341
Hpy188III TCNNGA 1 cut(s) 162
Hpy8I GTNNAC 2 cut(s) 188, 341
HpyAV CCTTC 2 cut(s) 20, 283
HpyCH4V TGCA 2 cut(s) 203, 377
Hsp92II CATG 1 cut(s) 254
Kzo9I GATC 1 cut(s) 277
LmnI GCTCC 2 cut(s) 29, 310
LpnPI CCDG 2 cut(s) 224, 251
Lsp1109I GCAGC 2 cut(s) 44, 47
MaeI CTAG 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 131
MalI GATC 1 cut(s) 279
MboI GATC 1 cut(s) 277
MboII GAAGA 3 cut(s) 74, 131, 239
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 3 cut(s) 86, 100, 144
MseI TTAA 2 cut(s) 57, 353
MslI CAYNNNNRTG 1 cut(s) 249
MspR9I CCNGG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 239
NdeII GATC 1 cut(s) 277
NlaIII CATG 1 cut(s) 254
NlaIV GGNNCC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 2 cut(s) 34, 37
PshBI ATTAAT 1 cut(s) 57
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 188
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 3 cut(s) 83, 320, 370
RsaNI GTAC 3 cut(s) 82, 319, 369
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 2 cut(s) 57, 353
SatI GCNGC 2 cut(s) 33, 36
Sau3AI GATC 1 cut(s) 277
Sau96I GGNCC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 1 cut(s) 363
SetI ASST 1 cut(s) 94
SinI GGWCC 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 249
Sse9I AATT 3 cut(s) 86, 100, 144
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 1 cut(s) 393
TasI AATT 3 cut(s) 86, 100, 144
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 2 cut(s) 57, 353
Tru9I TTAA 2 cut(s) 57, 353
TseI GCWGC 2 cut(s) 32, 35
TspDTI ATGAA 2 cut(s) 75, 239
TspGWI ACGGA 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 188
VspI ATTAAT 1 cut(s) 57
XapI RAATTY 2 cut(s) 100, 144
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.