Rmu_sc0001821.1_g000002

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001821.1
OG0002758
Physical Location & Seq
Reverse (-)
5367 .. 41950
36584 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 12450 bp
atgttcgaagcacacgtcctccatttgcttagaagatatttgggggaatatgtcgaaggactctctgtcgaagctttaagaataagtgtttggcaaggtgatgttgttctcaaagatttgaaattaaaggcagaggccctaaattcactaaaacttccagtcactgtaaaagctggttttattggtaccattacattaaaggttccatggaaaagtttgggcaaagaacctgtgattgttctcattgaccgtgtattcatccttgctcatcctgttactgatgacaggactctcaaggaggacagagacaaactatttgaagctaaacttcaagagattgaggaagcagagtcggcaactcttgaagcaatatcaaagtcaaagataggaagccaatctaatggaaattcatggctaggttcccttattgctactattattgggaatctaaagatatcaattagcaatgtacatataagatatgaggattcggtcagtaatccaggacatcctttttgttcgggtgccactttagccaaacttgctgctgttacgatggatgaggaagggaatgaaacatttgataccagtggtgctcttgataagttgcgaaagtcattgcaactagagagactagcaatgtatcatgattctgatagtgtaccgtggaagatagataagggatgggaggatcttactccaaaggaatgggtcgagatttttgaagatggaatacgtgaacctgtggatgaccatgcaatggtgtctaagtgggctatgaatcgcaagtatttggtctcacctataaatggagttctaaagtatcaccgtgtaggaaaccaagaaagaaatgatccagaggttccatttgagaacgcttcccttgccctaagtgacgtttccttgacaatgacagaggctcaatattgtgattggataaaactattggaagttgtttcaagatacaagagatatgttgaatttttccatctaagaccagcgctaccagtctctggggatccatatttgtggtggcgttatgctgctcaagcaggacttcagcagaaaaaaatgtgttaccggttctcttgggaccgcattcggcttctttgccagcttcgtcgccgttacattcagttatatgttggtttcttacaacacctgacaaatgttgataacgctgaaattagagaaattgagaaagatttggatcctaaagtaattcttctgtggaggttacttgcacatgcaaaggtcgaatctgtaaaatcgaaggaagcggctgaacagaggatgctccagaagaaaagctggttttcatttagatggcgtacacctgatgaatctgctgatgatacttctgagggatcacagtcaccagaagaaagactgactaaagaggagtggcaagcaattaataagctactgagttaccagccagatgaaacattggcatctcactcgggaaaggacatgccgaatatgatccggtttatggtaactgtgtctgttgatcgggctgctgctagaatcattgatataaatcacactgagattttgtgttgtaaatttgagcaacttcaagtgtcaacgaagtttaaacataaaagtacatactgtgatgtgtcattgagattctatggtctgtatgcaccggaaggctcacttgctcagagcgtatctagtgagcagaaggcgaatgcactggctgctagctttgtatactctccagttggagagaatgttgattggaggctttcagctaccatttcaccttgtcatgtcactgttttgatggaaagctgtgatcgctttttggattttgtgaaaaggagtaatgcagttagtcctactgttacattggaaacggcaactgctttgcagatgcaaattgagaaagtgactcgtagagcacaagagcaatttcaaatggtattagaagaacaaagcagatttgcccttgatattgatttggatgctccaaaagtaagagttcctatcagaaccaagggctcttccaaatgtgatagccattttctattggatcttggtcatttcaccctgcaaacgaaggatactcagcatgatgaacagaagaacatgtattctcgtttttttataacgggaagagagattgctgcattttttacggaatgtggttctgattgccaaattcgcactttggaatcacttgattctgataaccacccaccggttaccccggaaaatgttgacaatttctatcctctcatagataggtgtggaatggctcttcttgttgatcagatcaaagtacctcacccaagttacccgtcaatgcgtatttcaattcaagtgccaaaccttggggtgcacttttcaccatcgagatttcagagactcatgaaattattaaacatattttatggtactctggaaactgttggtcagccagctgttgataattttcaagcagaacgtgccccatggagtcctgcagatctttacacagatgctagaattttagtatggaaggggattggcaattctgtagctacatggcaaccttgtattcttgtgttgtcggggacaaatatttttgttttggagtctgagaagtcgcagacttatcagcgatactccagcatggctggtagacaagtatgtgaggttcctcctacaagtattggtggttcaccatttggccttgctgtgattcacagggggatggacactcaaaaggctctggagtcttctagtagcttgattattgagttccgtggggaggaggagaaggtcgtttggttgaaaagactcatacaagcaacatatcaagcttctgctcccccttcagttgatgtcttgggagaaacaattgatcgtgttgctgagtttggtgagcctcagaccggcaattctaaaacagctgaccttgtcattaatggggcattggtggagacaaagttatctatttatggaaagacgggtgataaagatgctgaagaacttgatgagactctgatgcttgaggttcttgccagtggggggcaggttcatatgattctttgggaaggtgacatgacacttaagacaaagcttcactctttaaaaatcaaagatgagcttcaaggtcgtctctctacaaccccacagtatttagcttgttctgtgctgaagagtgacaatttagtttcatcccccggcataattgatccacattggaaagaaatgtctgttttacttcatgaggaggatgatacttttacggatgctttgccagattttatgtctgtatcagatgcaggctttggttcaccaacttcagatacggtttcatgtgtaacaactgaggacatcaatgatgctgctggatttacatctgctgaaggcttagtccttgaaaagaatcttgttaaggcaaggtgcatttctgctgaggaattttatgaggcggagggtagtgactattcccattttgtatctctcaccttctcgactctgagttctagttcacctcattatgatggtattgacacacagatgagcatccgtatgtcaaaacttgaattcttctgcaacagacctactttggtcgctttaattgattttggcctggacttaagctctgtgtacagtgaggaaagtactgcagacattactgaaggttcaggtgacaaatctttgacgaacaaagaaaagactgaagaaattgggcgtgtcaaaggcttgctgggttatggtaaaggtcgtgtagtattttatttgaacatgaacgtggacagtgtgactgtgtttctcaacaaggaggatgcttctctgcttgcgacgtttgtgcaagaaagttttgtgctagatctgaaggtccacccaagttctctttccattgaaggcaccctaggaaattttagactccgtgatatgtctcttggagaagataactgttgggcttggctatgtgacatacgcaatccagacgtggaatcccttatcaagttcaactttaagtcttacagtgctgaagacgatgattatgaaggatatgattatagtttgtgtggtcgatttgctgctgttcgtattgtcttcctttacaggtttgttcaggagataactgcatactttatggaacttgctaccccacatactgaagatgcaatcaaacttgttgataaggttgggggttttgagtggctaattcagaagtatgagatggatggagctgccgcacttaagctggatctgtcactcgatactccaataatcattgttcccaggaattcaactagcaatgatttcattcaacttgatctgggccagctacaagtcacaaatgaatttagttggcatggttctccagagaaagatccttctgcagtccacattgatgtacttcatgctgagattctggggatcaacatgtctgtaggaatcgacggtcgcatgggtaaatcaatgatacgagaaggcaaaggatttgatgtttatgtccggagaagtttgagggatatcttcaaaaaagtcccaacattttctcttgaagtgaaggtgggcttattgcattgcgtcatgtctgacaaagaatacaaagtcattctggattgtgcatacacgaacttatgtgaagaaccaaagcttccccctagtttccgtggtggaatatctgattcaaaggatacaattaggctgctggttgataaggtcaacatgaatagtcaaatcctactatcgcggactgtttcgatagtggcggttgtggttgatcatgcactgttggagttgtataatggtattcatgtggagtctcctttcgctcaaattgctcttgaaggtctttgggtgtcgtatcggatgacttcattgtctgaaacagatctgtacataacaatttcaaaattttctgttgtggacattcgccctgatacaaaacctgaaatgcgccttatgcttggatcatccacggatgcttttaaacaagtttcctctggaagtttgcctttcttcttaaatagggggagctttaggagaactgactctgatgctggattccatggggacttaccaatatcaacaatgttcttaatggactatagatggcgcacatcaacccaattgtttgtggttaggattcagcaacctcgagttctagttgtagccgatttccttttggctgtgggcgagttttttgttcctgcattgcggacaataactggtagggaggaagtgatggatcctcataatgatcctattggtaaaaacagtagcatagtgttcacagagcctacatacaaacagacagaagatgtggtgcatttgtctccaagtagacagttagtagcagattttttaagcatcgatgaatatacatatgatggttgtggaaagaccatccatttgagtgaggaaatagatgctaaagaactccattcaactagacctcgaccaataattataattggacgtggaaaaaggttgcggtttatgaatgtcaaaattgagaatgggtctcttttgaggaagtatacatacttaaataatgatagcagctactcagtagcctttgaggatggtgttgacattacgttgctggaaagttcctcatctgatgatgataagaagagtctagaatacttccatgaatcgccagactcctcaaatatttcctcaaattctgaaagtgatcctaataagatcccaagtttcagttttgaaacacaggttgtttctcctgagtttactttttatgatggttcaaagtcgtctctggatgattcatatggtgaaaagcttctccgtgcaaagctggattttagcttcatgtatgcatctaaagaaaatgatacctgggttcgagctctggtgaaggatcttactgttgaggctggttctgggcttgttgtgcttgatccagtggatatatcagggggatacacttctgttaaggacaaaacaaatatgtccttgttatcaactgatatccgcttccatctatcactaagtgttatatctcttataagtaatttgcaaagccaggcaacagctgcactgcaatatggaaattcagcacccttggctccttgtaccaacttcgatcgaatttgggtatccccaaaagaaaatgggtcctgtttcaaccttacgttttggaggccgcgagcaccttcaaattttgttattttgggagattgtgtgacatcaaagccagttcctccctcacaggcagtaatggcagtgagtaatacatatgggcgtgtgtgtaaaccaactggtttcaatctcataggattgttctctgcaattcaaggatttacggggggtgactctgatattaatacagattgttctctttggatgcctatagcaccaccaggatacacagcattgggttctgtagcaaatattgggaaggaacccccacccaaccacattgtttattgcattcgttccgatcttgtaacatcaacaacctatatagaatgcttgttctgctctgcatcaaatcctcaatttgcatctggatttagcatctggcgtgtagagaatgtgcttggatcattttatgcccattcttctactgaatgtccttcccaagaccgttgttgtaacctcaatcatcttcttctttggaactcaagtcggcatcattcttctgctaaagaacctgcctcagatttagctgttgttgaaaaccatgaaagtcaggtaagcagaaaccagagtgcacattcttctggatgggatattgtcagatcaagttcaaggacaactaaatgttatatgtctacccctaactttgagagaatctggtgggacaagggtagtgacattcgccgaccagtctcaatatggagacccattcgtcgtcaaggctatgccatcttgggtgattgcattactgaaggcttagaaccaccagctgttggcataatatttagagctgatgattgtgaaatatctgcgaaacccgtgcaatttactaaagttgcccatgtggtagggaaaggttttgatgaagctttcttctggtacccaattgctccaccaggttatgcttctctaggatgcatagtctccagaatagatgaacccccaggtgtaaactcattttgctgccctaggatggatctggttaaccaagctaatattcttgaggtgcccatttcaagatcttcagcttcaaagggttctcagtgctggagcatctggagagttgaaaatcaggcatgcacttttcttgcacgtggtgatctgaaaaaaccgtctagcagattggcttatgttattggtgactccatgaaacccaagactaaggaaaacataactgctgaggtgaagctaagatgcttgtctgtaactgttctagacagcttgtgtggaatgatgaccccactatttgacacaacgatcaccaatatcaaacttgcaacacatggtcgagtagaagccatgaatgcagtactaatttcctctattgctgcatcaaccttcaatactcagttggaagcgtgggagccgcttgtggaaccttttgatggaatattcaagtttgaaacatatgattctaatgtacactcgccatcagaatttgggaagacaatgcgtattgctgccacaagtatcgttaatataaatgtcagcgccgcaaatcttgaaacttttgtagggactattctgtcatggagaagacaattagaccttgaacagaaagcaaagaaggaagcttgtggtctaagcggtaaaggagaggaccagactttgtctgcactggatgatgacgactttcagactgtgatagtagagaataaacttggttgtgatgtctatctgaaaaaagttgaacaaaattcagatatggtgaaccagttgcatcatggtgactctgtctctgtatgggttccacctccacagttttcagacaggttaaatgttgcagatgaatccaaggaagctcgttattatgttgctatacaaattcatgaagctaagggtttgcctattacagatgatggtaatagccataacttcttatgtgctttgagacttgctgttgataaccaagcaacagatcaacaaaaactttttcctcaaagtgcaaggacaaaatgcgttaagccttcagtttcgaaaaatagtaacctgaatgaaggctctgcagaatggaatgaacttttcatatttgaagttcctcggaagggcccagctaaactggaagtggaggtgacaaacctcgcagcgaaagctggaaagggtgaagtagttggtgcactctcattttctgttgggcaagatgcgaatatgttgaggaagctagcctcagtgaaggttctccatcaaggtcatgatattcagaatatagtatcctatccgctaaagcagacggttcaacagaaccaacttgaagacccggacgaatatggttgcctgttggtttcaacttcgtattttgagaggaaaacaactccaagtcttcaaagagatttggagaatgagaatgtagttgatagagacataggtttctatattggtctgggccccaaaggtgtttggcagggcattcgggcgttgcttccattgtcagttgttccaaaattgttacaggatgattttattgcactggaagttgcgctgaaaaatggcaggaaacatgctattttcaggggtcttgcaacagttgtaaatgagactgatgttacattgaaactttcagtatgtggtgcttctctgattcaagtttatgattcctcatctggaacaagtggaaacataaatctccctaggaatgatgtctctgccataaaccctggaggttcatttgttttaccttggaaaagtacatcaagtgattctgaccagtgcttacaaatttgcccttctgttgatcatcctcagcgtccatattcctggggttctgtggttgcagtgggatctggttatgcatctggaaaggacttgacggttatggatcaggtttcactttcaggacagtatacttcaaagcaagaaattatgatgcctaatgttacttttcagttgaatcagcttgagaagaaagataaacttctgtgttgtactagtaacgtaaataaacaactttggcttagtgttggggcagatgcctcagttcttcatactgaactcaatgcacctgtttatgattggagaatctctgtcaattctcctatgaagttggaaaacagacttccctgtccggctgaattcaccatttgggaaaagataaaagaaggaaagtgtattgagcgccagaatggtatgatctcttcacgtggcggtgttcatatatattcagcagacattcagaaaccgatatatctcaccttgtttgttcagggtggctgggtcatggagaagggccccattcttgttttgaatctatatactaatgatcatgtctcatcattctggatggttcaccagaaaagtaaaaggagactgcgagtgagtattgaacgtgacatgggagggaccactgttgcacccaaaatcataaggttttttgttccttattggatcattaatgagtcatctatttctttggcgtatcgagtggtggaagtggagcctttggacaatgcagacccagattccccatttgtttctagagcagtcaactctgcaaagacagctttaaaaagtcctacaatctccatggaaaggaagcattcagctgcaaggagaaacattcaagtacttgaagttattgaggatactagtcctgttcctaacatgctttctccgcaagactacaaaagtcgcagtggagctatgctgttttcatctcaaaaagatgtgtatccgtcctctcgagttggcctttctgttgcgattcgccattcggaaagttacagttctggaatatctcttcatgagcttgaaaagaaggaacggcttgatgtaaaagcattcagttcagatggatcttattacatgctttcagcgcgcttgaacatgacctcagacagaacaaaggttgtgcacttccaaccccacacattgttcgtgaatagagttggttacagcctctgcttgcagcagtgtgattctcaggcagtgacatggattcatcccactgattctccaaaacccttctgttggcagtctggttccaaatttgaaaagttgaagctgcgggtggatggttacaaatggagtacaccatttagtgtttgtaatgaaggtattatgcgtgtttgcttgaggaaagatactggaaacgatcaactgcagttacgagttggagtaagaagtggagcaaagaactcaagcctggaagttattttccgtccaaactcttccttgagtccttacaggattgaaaatcgttccatgtttttacccatccgtattcggcaagtggacggtacctttgattcatggactttccttcttcctaattcagcagcttcctttttatgggaagatcttggtagaagacgactattagaaatgctggtagagggggcggacccattgaaatcagagatgtatgatattgatgagatttttgatcatcaacccattaaagtgggaagtggatcctctagagctctgcgtgttaccatagtaaaagaagagaaagttaatgttatcaagatcagtgactggatgccagaaagtgaacctactggaatcttaagtagaaggcatttgtcatccctctctcagctttctaaacagcaacaaacagcatcttcctctgattgtgaatttcatattattgttgagttagctgaacttgggatatccattattgaccatacacctgaagaaatcctgtacctttcgattcagaatcttctttttgcatattcaaccggcttgggctctggagtcagtaggttaaaacttagaatgtgtgggatacaattggacaaccaattgccattaaccccaatgccagttctctttaggccacatagagttggagaggagatggactatatcttgaaattttctgtcacaatgcaatccaatgggtcactggatctttgtgtctatccgtatattggcctccacggacctgaaaattctgcctttttgataaacattcatgaacctatcatctggcgccttcatgaaatgattcagcaggtcaaccttagtcggttgtatgatacccaaacaactgccgtttctgttgatccaatcgttgaaattggggttcttaacatatcagaggttcgattcaaaatgtcaatggctatgtcacctagtcagcggccaagaggtgttcttggattttgggcatctttaatgactgcattagggaacactgagaatatggcagtgaggatcaatcaaaggtttcttgaaaatgtgtgcatgaggcagagttccatgattagtattgctatttcaaacattcaaaaggatcttttaggccagcctctacaactgctctcgggtgtcgatatactgggtaatgcaagtagtgcacttggacatatgagcaggggtgtggctgccttatcatttgataagaagttcatacaaagccgacaaagacaggaaagcaagggtgtagaggactttggtgatgttcttagagaaggaggtggagcactggcaaaaggcttcttcagaggggtcactggcatattgacaaagccacttgaaggtgctaagtcttctggtgttgagggttttgttcaaggtgttggaaaaggattaattggtgcagctgcacaaccggtgagtggggttctggatcttctgtcaaaaactactgaaggtgctaatgcaatgagaatgaagataacatcagctataacttctgaagatcaactcctccgtagacgactgccgcgtgtaattagtggtgataatttacttagaccatatgacgactacaaagctcaaggacaggttatattgcagctggcagaatcaggttcattttttctccaggttgatctatttaaagtacgtggaaagtttgcgttatcagatgcttatgaagatcactttttaatccgcaaaggaaaagttcttgtggtcactcatcgaagagtgatactgctgcaacaacccaatactatttctcagaagaagttcaacgcagctagggatccctgttcggtattgtgggatgtactttgggatgacctggtgacaatggaactggcatttggaaaaaaagaccacccaaaagctccacattcacggctcattctttatttacgtgatagatcaaccgagatgagggagcaagctcgtgtaataaagtgcatccatgacaccccccaggcttttgaagtgtacacctcaattgaacgagcaatgagcatttatggaccaaacaaaacaaagggaaggccaacaaaaaatgtgacaaagccatattcaccacttgctgatagcaccggtgctgaagttaacccaaaagaagggcttagctccttgtcaccccgaccgatgcaacttcctcgttcaatgtttggcaggagtgccaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

4149

Amino Acids

463.12

Weight (kDa)

5.89

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 1 - 701 1.8e-99 VPS13-like family N-terminal region
ATG2_CAD PF13329 8 - 165 3.2e-08 Autophagy-related protein 2
VPS13_M PF25033 1401 - 1539 6.3e-07 VPS13-like family middle region
Vps62 PF06101 2001 - 2171 1.5e-10 Vacuolar protein sorting-associated protein 62
Vps62 PF06101 2256 - 2397 8.8e-09 Vacuolar protein sorting-associated protein 62
VPS13_VAB PF25036 3007 - 3442 2.7e-22 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_C PF25037 3931 - 4038 2e-12 Intermembrane lipid transfer protein VPS13, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2120, 5429, 5990
AasI GACNNNNNNGTC 4 cut(s) 4837, 6834, 7648, 9180
Acc36I ACCTGC 3 cut(s) 3019, 6646, 11074
Acc65I GGTACC 3 cut(s) 185, 7002, 10355
AccB1I GGYRCC 7 cut(s) 185, 524, 3869, 7002, 7129, 10355, 11061
AccB7I CCANNNNNTGG 4 cut(s) 760, 1013, 2345, 6896
AccI GTMKAC 7 cut(s) 1722, 2635, 5302, 5498, 6758, 8963, 11788
AccII CGCG 4 cut(s) 4708, 6130, 9934, 11800
AccIII TCCGGA 1 cut(s) 4467
AcoI YGGCCR 1 cut(s) 11213
AcvI CACGTG 2 cut(s) 7217, 9260
AcyI GRCGYC 1 cut(s) 11062
AdeI CACNNNGTG 2 cut(s) 5975, 7220
AfeI AGCGCT 1 cut(s) 1002
AflII CTTAAG 4 cut(s) 3065, 3598, 4208, 10627
AflIII ACRYGT 3 cut(s) 13, 2100, 4395
AgeI ACCGGT 4 cut(s) 1080, 2212, 11683, 12356
AhdI GACNNNNNGTC 1 cut(s) 65
AhlI ACTAGT 2 cut(s) 9047, 9704
AjiI CACGTC 3 cut(s) 16, 3955, 5438
AjuI GAANNNNNNNTTGG 8 cut(s) 73, 105, 3841, 3873, 7104, 7136, 12105, 12137
Alw44I GTGCAC 5 cut(s) 2351, 6696, 8238, 9968, 11427
AlwNI CAGNNNCTG 4 cut(s) 164, 1013, 10017, 10596
Ama87I CYCGRG 4 cut(s) 1462, 5115, 9798, 11395
Aor13HI TCCGGA 1 cut(s) 4467
Aor51HI AGCGCT 1 cut(s) 1002
ApaI GGGCCC 3 cut(s) 8174, 8510, 9350
ApaLI GTGCAC 5 cut(s) 2351, 6696, 8238, 9968, 11427
ArsI GACNNNNNNTTYG 9 cut(s) 32, 299, 331, 498, 530, 5353, 5385, 10343, 10375
AseI ATTAAT 5 cut(s) 1416, 2919, 6306, 9510, 11663
AsiGI ACCGGT 4 cut(s) 1080, 2212, 11683, 12356
Asp700I GAANNNNTTC 3 cut(s) 5763, 11076, 12044
Asp718I GGTACC 3 cut(s) 185, 7002, 10355
AspA2I CCTAGG 3 cut(s) 3874, 7091, 8750
AspLEI GCGC 9 cut(s) 1003, 4917, 5076, 7616, 8602, 9237, 9934, 9936, 11064
AsuC2I CCSGG 3 cut(s) 2222, 3180, 8381
AsuII TTCGAA 2 cut(s) 6, 8099
AsuNHI GCTAGC 2 cut(s) 1712, 8284
AvaI CYCGRG 4 cut(s) 1462, 5115, 9798, 11395
AvaII GGWCC 8 cut(s) 1093, 3841, 6099, 7723, 9459, 10459, 11012, 12287
AvrII CCTAGG 3 cut(s) 3874, 7091, 8750
BaeI ACNNNNGTAYC 2 cut(s) 5808, 5841
BamHI GGATCC 5 cut(s) 1018, 1210, 5206, 10529, 12061
BanI GGYRCC 7 cut(s) 185, 524, 3869, 7002, 7129, 10355, 11061
BanII GRGCYC 7 cut(s) 2015, 5833, 8174, 8510, 9350, 10543, 10820
BarI GAAGNNNNNNTAC 6 cut(s) 717, 749, 5486, 5518, 9238, 9270
BauI CACGAG 1 cut(s) 12207
BbrPI CACGTG 2 cut(s) 7217, 9260
BbsI GAAGAC 9 cut(s) 2724, 4005, 4054, 7574, 7666, 8382, 8435, 10432, 11613
BbvCI CCTCAGC 3 cut(s) 3414, 7302, 8862
BceAI ACGGC 5 cut(s) 1110, 1884, 9896, 11108, 12173
BciVI GTATCC 9 cut(s) 2068, 4645, 5897, 6091, 6341, 8344, 9694, 9798, 10848
BclI TGATCA 5 cut(s) 2281, 4738, 8854, 9379, 10501
BcnI CCSGG 3 cut(s) 2222, 3180, 8381
BcuI ACTAGT 2 cut(s) 9047, 9704
BfoI RGCGCY 4 cut(s) 1004, 7617, 9238, 11065
BfrI CTTAAG 4 cut(s) 3065, 3598, 4208, 10627
BfuAI ACCTGC 3 cut(s) 3019, 6646, 11074
BfuI GTATCC 9 cut(s) 2068, 4645, 5897, 6091, 6341, 8344, 9694, 9798, 10848
BglII AGATCT 5 cut(s) 2479, 3832, 4849, 7142, 10414
BlnI CCTAGG 3 cut(s) 3874, 7091, 8750
BlpI GCTNAGC 1 cut(s) 12386
BmcAI AGTACT 3 cut(s) 3625, 7434, 9684
Bme18I GGWCC 8 cut(s) 1093, 3841, 6099, 7723, 9459, 10459, 11012, 12287
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 4 cut(s) 1462, 5115, 9798, 11395
BmgBI CACGTC 3 cut(s) 16, 3955, 5438
BmrI ACTGGG 1 cut(s) 11420
BmtI GCTAGC 2 cut(s) 1716, 8288
BmuI ACTGGG 1 cut(s) 11420
BpiI GAAGAC 9 cut(s) 2724, 4005, 4054, 7574, 7666, 8382, 8435, 10432, 11613
Bpu10I CCTNAGC 4 cut(s) 3414, 7302, 7959, 8862
Bpu1102I GCTNAGC 1 cut(s) 12386
Bpu14I TTCGAA 2 cut(s) 6, 8099
BpuMI CCSGG 3 cut(s) 2222, 3180, 8381
Bsa29I ATCGAT 1 cut(s) 5331
BsaAI YACGTR 5 cut(s) 737, 7217, 9260, 11921, 12176
BsaBI GATNNNNATC 6 cut(s) 1209, 1542, 3355, 3524, 7797, 9786
BsaHI GRCGYC 1 cut(s) 11062
BsaI GGTCTC 3 cut(s) 802, 5487, 6820
BsaWI WCCGGW 7 cut(s) 1080, 1488, 1654, 2212, 4467, 11683, 12356
BsaXI ACNNNNNCTCC 9 cut(s) 33, 1728, 1758, 2927, 2957, 6150, 6180, 10054, 10084
Bse118I RCCGGY 6 cut(s) 1080, 2212, 2888, 10808, 11683, 12356
Bse3DI GCAATG 9 cut(s) 472, 617, 645, 765, 4273, 4537, 5171, 11742, 12279
Bse8I GATNNNNATC 6 cut(s) 1209, 1542, 3355, 3524, 7797, 9786
BseAI TCCGGA 1 cut(s) 4467
BseCI ATCGAT 1 cut(s) 5331
BseJI GATNNNNATC 6 cut(s) 1209, 1542, 3355, 3524, 7797, 9786
BseMI GCAATG 9 cut(s) 472, 617, 645, 765, 4273, 4537, 5171, 11742, 12279
BsePI GCGCGC 1 cut(s) 9932
BseRI GAGGAG 7 cut(s) 1415, 2780, 2783, 3242, 5617, 10937, 11771
BseYI CCCAGC 3 cut(s) 3709, 8173, 9330
BsgI GTGCAG 4 cut(s) 6003, 7722, 11661, 11691
Bsh1236I CGCG 4 cut(s) 4708, 6130, 9934, 11800
Bsh1285I CGRYCG 3 cut(s) 4417, 6070, 12407
BshNI GGYRCC 7 cut(s) 185, 524, 3869, 7002, 7129, 10355, 11061
BshTI ACCGGT 4 cut(s) 1080, 2212, 11683, 12356
BshVI ATCGAT 1 cut(s) 5331
BsiEI CGRYCG 3 cut(s) 4417, 6070, 12407
BsiHKCI CYCGRG 4 cut(s) 1462, 5115, 9798, 11395
BslFI GGGAC 7 cut(s) 1106, 2581, 4484, 5045, 6800, 7654, 9472
BsmBI CGTCTC 2 cut(s) 3119, 5742
BsmFI GGGAC 7 cut(s) 1106, 2581, 4484, 5045, 6800, 7654, 9472
BsmI GAATGC 8 cut(s) 1098, 1705, 6414, 6458, 7432, 8529, 9655, 9896
Bso31I GGTCTC 3 cut(s) 802, 5487, 6820
BsoBI CYCGRG 4 cut(s) 1462, 5115, 9798, 11395
Bsp119I TTCGAA 2 cut(s) 6, 8099
Bsp120I GGGCCC 3 cut(s) 8170, 8506, 9346
Bsp13I TCCGGA 1 cut(s) 4467
Bsp1407I TGTACA 5 cut(s) 469, 3609, 4854, 7543, 12252
Bsp1720I GCTNAGC 1 cut(s) 12386
Bsp19I CCATGG 4 cut(s) 206, 2465, 5026, 9642
BspDI ATCGAT 1 cut(s) 5331
BspEI TCCGGA 1 cut(s) 4467
BspFNI CGCG 4 cut(s) 4708, 6130, 9934, 11800
BspHI TCATGA 8 cut(s) 646, 2382, 3223, 7951, 8314, 9859, 11044, 11068
BspMAI CTGCAG 5 cut(s) 2479, 3631, 4354, 8131, 10221
BspMI ACCTGC 3 cut(s) 3019, 6646, 11074
BspOI GCTAGC 2 cut(s) 1716, 8288
BspQI GCTCTTC 2 cut(s) 2020, 2277
BspT104I TTCGAA 2 cut(s) 6, 8099
BspT107I GGYRCC 7 cut(s) 185, 524, 3869, 7002, 7129, 10355, 11061
BspTI CTTAAG 4 cut(s) 3065, 3598, 4208, 10627
BspTNI GGTCTC 3 cut(s) 802, 5487, 6820
BsrDI GCAATG 9 cut(s) 472, 617, 645, 765, 4273, 4537, 5171, 11742, 12279
BsrFI RCCGGY 6 cut(s) 1080, 2212, 2888, 10808, 11683, 12356
BsrGI TGTACA 5 cut(s) 469, 3609, 4854, 7543, 12252
BssAI RCCGGY 6 cut(s) 1080, 2212, 2888, 10808, 11683, 12356
BssHII GCGCGC 1 cut(s) 9932
BssNAI GTATAC 3 cut(s) 1723, 5499, 8964
BssNI GRCGYC 1 cut(s) 11062
BssSI CACGAG 1 cut(s) 12207
Bst1107I GTATAC 3 cut(s) 1723, 5499, 8964
Bst2BI CACGAG 1 cut(s) 12207
BstACI GRCGYC 1 cut(s) 11062
BstAFI CTTAAG 4 cut(s) 3065, 3598, 4208, 10627
BstAPI GCANNNNNTGC 4 cut(s) 1709, 2459, 6017, 11426
BstAUI TGTACA 5 cut(s) 469, 3609, 4854, 7543, 12252
BstBAI YACGTR 5 cut(s) 737, 7217, 9260, 11921, 12176
BstBI TTCGAA 2 cut(s) 6, 8099
BstDSI CCRYGG 9 cut(s) 206, 665, 2465, 2758, 4627, 4935, 5026, 9642, 11008
BstEII GGTNACC 1 cut(s) 2215
BstFNI CGCG 4 cut(s) 4708, 6130, 9934, 11800
BstH2I RGCGCY 4 cut(s) 1004, 7617, 9238, 11065
BstHHI GCGC 9 cut(s) 1003, 4917, 5076, 7616, 8602, 9237, 9934, 9936, 11064
BstMCI CGRYCG 3 cut(s) 4417, 6070, 12407
BstNSI RCATGY 8 cut(s) 1250, 1477, 2104, 4399, 7203, 8624, 9724, 9925
BstPI GGTNACC 1 cut(s) 2215
BstUI CGCG 4 cut(s) 4708, 6130, 9934, 11800
BstV2I GAAGAC 9 cut(s) 2724, 4005, 4054, 7574, 7666, 8382, 8435, 10432, 11613
BstXI CCANNNNNNTGG 5 cut(s) 401, 708, 1029, 8877, 10519
BstZ17I GTATAC 3 cut(s) 1723, 5499, 8964
Bsu15I ATCGAT 1 cut(s) 5331
BsuI GTATCC 9 cut(s) 2068, 4645, 5897, 6091, 6341, 8344, 9694, 9798, 10848
BsuTUI ATCGAT 1 cut(s) 5331
BtgI CCRYGG 9 cut(s) 206, 665, 2465, 2758, 4627, 4935, 5026, 9642, 11008
BtrI CACGTC 3 cut(s) 16, 3955, 5438
BtsI GCAGTG 7 cut(s) 6020, 6213, 8901, 9757, 10034, 10050, 11286
BveI ACCTGC 3 cut(s) 3019, 6646, 11074
CaiI CAGNNNCTG 4 cut(s) 164, 1013, 10017, 10596
CciI TCATGA 8 cut(s) 646, 2382, 3223, 7951, 8314, 9859, 11044, 11068
CfoI GCGC 9 cut(s) 1003, 4917, 5076, 7616, 8602, 9237, 9934, 9936, 11064
Cfr10I RCCGGY 6 cut(s) 1080, 2212, 2888, 10808, 11683, 12356
ClaI ATCGAT 1 cut(s) 5331
CseI GACGC 2 cut(s) 4531, 8855
CsiI ACCWGGT 2 cut(s) 7018, 12099
CspAI ACCGGT 4 cut(s) 1080, 2212, 11683, 12356
DinI GGCGCC 1 cut(s) 11063
DraI TTTAAA 5 cut(s) 1600, 3087, 4948, 9624, 11914
DraIII CACNNNGTG 2 cut(s) 5975, 7220
DrdI GACNNNNNNGTC 4 cut(s) 4837, 6834, 7648, 9180
DriI GACNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 4 cut(s) 4837, 6834, 7648, 9180
EaeI YGGCCR 1 cut(s) 11213
Eam1105I GACNNNNNGTC 1 cut(s) 65
EciI GGCGGA 2 cut(s) 3446, 10472
Ecl136II GAGCTC 2 cut(s) 5831, 10541
Eco24I GRGCYC 7 cut(s) 2015, 5833, 8174, 8510, 9350, 10543, 10820
Eco31I GGTCTC 3 cut(s) 802, 5487, 6820
Eco32I GATATC 4 cut(s) 456, 4486, 5953, 10737
Eco47I GGWCC 8 cut(s) 1093, 3841, 6099, 7723, 9459, 10459, 11012, 12287
Eco47III AGCGCT 1 cut(s) 1002
Eco53kI GAGCTC 2 cut(s) 5831, 10541
Eco72I CACGTG 2 cut(s) 7217, 9260
Eco88I CYCGRG 4 cut(s) 1462, 5115, 9798, 11395
Eco91I GGTNACC 1 cut(s) 2215
EcoICRI GAGCTC 2 cut(s) 5831, 10541
EcoO109I RGGNCCY 6 cut(s) 136, 6099, 8170, 8507, 9346, 9347
EcoO65I GGTNACC 1 cut(s) 2215
EcoRI GAATTC 3 cut(s) 3545, 4255, 9191
EcoRV GATATC 4 cut(s) 456, 4486, 5953, 10737
EcoT22I ATGCAT 3 cut(s) 5803, 7043, 8914
EcoT38I GRGCYC 7 cut(s) 2015, 5833, 8174, 8510, 9350, 10543, 10820
EgeI GGCGCC 1 cut(s) 11063
EheI GGCGCC 1 cut(s) 11063
Esp3I CGTCTC 2 cut(s) 3119, 5742
FaqI GGGAC 7 cut(s) 1106, 2581, 4484, 5045, 6800, 7654, 9472
FauI CCCGC 1 cut(s) 10116
FauNDI CATATG 7 cut(s) 3036, 5344, 5752, 6220, 7531, 11439, 11833
FbaI TGATCA 5 cut(s) 2281, 4738, 8854, 9379, 10501
FblI GTMKAC 7 cut(s) 1722, 2635, 5302, 5498, 6758, 8963, 11788
FriOI GRGCYC 7 cut(s) 2015, 5833, 8174, 8510, 9350, 10543, 10820
GlaI GCGC 9 cut(s) 1002, 4916, 5075, 7615, 8601, 9236, 9933, 9935, 11063
GsaI CCCAGC 3 cut(s) 3713, 8177, 9334
HaeII RGCGCY 4 cut(s) 1004, 7617, 9238, 11065
HgaI GACGC 2 cut(s) 4531, 8855
HhaI GCGC 9 cut(s) 1003, 4917, 5076, 7616, 8602, 9237, 9934, 9936, 11064
Hin1I GRCGYC 1 cut(s) 11062
Hin6I GCGC 9 cut(s) 1001, 4915, 5074, 7614, 8600, 9235, 9932, 9934, 11062
HinP1I GCGC 9 cut(s) 1001, 4915, 5074, 7614, 8600, 9235, 9932, 9934, 11062
HincII GTYRAC 8 cut(s) 1590, 2233, 4681, 5551, 7108, 9604, 11089, 12370
HindII GTYRAC 8 cut(s) 1590, 2233, 4681, 5551, 7108, 9604, 11089, 12370
HindIII AAGCTT 7 cut(s) 72, 2814, 3074, 4610, 5762, 6990, 7695
HpaI GTTAAC 2 cut(s) 7108, 12370
Hpy99I CGWCG 4 cut(s) 1125, 3808, 4415, 6840
Hsp92I GRCGYC 1 cut(s) 11062
HspAI GCGC 9 cut(s) 1001, 4915, 5074, 7614, 8600, 9235, 9932, 9934, 11062
KasI GGCGCC 1 cut(s) 11061
Kpn2I TCCGGA 1 cut(s) 4467
KpnI GGTACC 3 cut(s) 189, 7006, 10359
Ksp22I TGATCA 5 cut(s) 2281, 4738, 8854, 9379, 10501
KspAI GTTAAC 2 cut(s) 7108, 12370
LguI GCTCTTC 2 cut(s) 2020, 2277
MabI ACCWGGT 2 cut(s) 7018, 12099
MfeI CAATTG 6 cut(s) 2853, 5087, 7008, 10859, 10871, 12261
Mly113I GGCGCC 1 cut(s) 11062
MmeI TCCRAC 8 cut(s) 1714, 4731, 7455, 9144, 10000, 10210, 10897, 11632
Mph1103I ATGCAT 3 cut(s) 5803, 7043, 8914
MroI TCCGGA 1 cut(s) 4467
MroXI GAANNNNTTC 3 cut(s) 5763, 11076, 12044
MspA1I CMGCKG 8 cut(s) 2435, 2906, 6017, 6893, 9662, 11212, 11675, 11872
MspCI CTTAAG 4 cut(s) 3065, 3598, 4208, 10627
MssI GTTTAAAC 1 cut(s) 1600
MunI CAATTG 6 cut(s) 2853, 5087, 7008, 10859, 10871, 12261
Mva1269I GAATGC 8 cut(s) 1098, 1705, 6414, 6458, 7432, 8529, 9655, 9896
MvnI CGCG 4 cut(s) 4708, 6130, 9934, 11800
NarI GGCGCC 1 cut(s) 11062
NciI CCSGG 3 cut(s) 2222, 3180, 8381
NcoI CCATGG 4 cut(s) 206, 2465, 5026, 9642
NdeI CATATG 7 cut(s) 3036, 5344, 5752, 6220, 7531, 11439, 11833
NheI GCTAGC 2 cut(s) 1712, 8284
NsiI ATGCAT 3 cut(s) 5803, 7043, 8914
NspI RCATGY 8 cut(s) 1250, 1477, 2104, 4399, 7203, 8624, 9724, 9925
NspV TTCGAA 2 cut(s) 6, 8099
PaeI GCATGC 1 cut(s) 7203
PaeR7I CTCGAG 2 cut(s) 5115, 9798
PagI TCATGA 8 cut(s) 646, 2382, 3223, 7951, 8314, 9859, 11044, 11068
PauI GCGCGC 1 cut(s) 9932
PciI ACATGT 2 cut(s) 2100, 4395
PciSI GCTCTTC 2 cut(s) 2020, 2277
PcsI WCGNNNNNNNCGW 2 cut(s) 12, 11797
PctI GAATGC 8 cut(s) 1098, 1705, 6414, 6458, 7432, 8529, 9655, 9896
PdmI GAANNNNTTC 3 cut(s) 5763, 11076, 12044
PflFI GACNNNGTC 3 cut(s) 2912, 7732, 7856
PflMI CCANNNNNTGG 4 cut(s) 760, 1013, 2345, 6896
PfoI TCCNGGA 1 cut(s) 502
PinAI ACCGGT 4 cut(s) 1080, 2212, 11683, 12356
Ple19I CGATCG 1 cut(s) 6070
PluTI GGCGCC 1 cut(s) 11065
PmaCI CACGTG 2 cut(s) 7217, 9260
PmeI GTTTAAAC 1 cut(s) 1600
PmlI CACGTG 2 cut(s) 7217, 9260
Ppu21I YACGTR 5 cut(s) 737, 7217, 9260, 11921, 12176
PpuMI RGGWCCY 1 cut(s) 6099
PscI ACATGT 2 cut(s) 2100, 4395
PshBI ATTAAT 5 cut(s) 1416, 2919, 6306, 9510, 11663
PsiI TTATAA 3 cut(s) 2120, 5429, 5990
Psp124BI GAGCTC 2 cut(s) 5833, 10543
Psp5II RGGWCCY 1 cut(s) 6099
PspCI CACGTG 2 cut(s) 7217, 9260
PspEI GGTNACC 1 cut(s) 2215
PspFI CCCAGC 3 cut(s) 3709, 8173, 9330
PspOMI GGGCCC 3 cut(s) 8170, 8506, 9346
PspPPI RGGWCCY 1 cut(s) 6099
PspXI VCTCGAGB 1 cut(s) 5115
PsrI GAACNNNNNNTAC 4 cut(s) 2634, 2656, 2666, 2688
PstI CTGCAG 5 cut(s) 2479, 3631, 4354, 8131, 10221
PstNI CAGNNNCTG 4 cut(s) 164, 1013, 10017, 10596
PsyI GACNNNGTC 3 cut(s) 2912, 7732, 7856
PteI GCGCGC 1 cut(s) 9932
PvuI CGATCG 1 cut(s) 6070
PvuII CAGCTG 7 cut(s) 2435, 2906, 6017, 6893, 9662, 11675, 11872
SacI GAGCTC 2 cut(s) 5833, 10543
SapI GCTCTTC 2 cut(s) 2020, 2277
ScaI AGTACT 3 cut(s) 3625, 7434, 9684
SexAI ACCWGGT 2 cut(s) 7018, 12099
SfoI GGCGCC 1 cut(s) 11063
Sfr274I CTCGAG 2 cut(s) 5115, 9798
SfuI TTCGAA 2 cut(s) 6, 8099
SgrAI CRCCGGYG 1 cut(s) 12356
SinI GGWCC 8 cut(s) 1093, 3841, 6099, 7723, 9459, 10459, 11012, 12287
SlaI CTCGAG 2 cut(s) 5115, 9798
SpeI ACTAGT 2 cut(s) 9047, 9704
SphI GCATGC 1 cut(s) 7203
SspDI GGCGCC 1 cut(s) 11061
SspI AATATT 7 cut(s) 926, 2575, 5635, 6376, 6906, 7120, 7515
SstI GAGCTC 2 cut(s) 5833, 10543
TaqII GACCGA 2 cut(s) 481, 12421
TauI GCSGC 7 cut(s) 1283, 4205, 6130, 7492, 7619, 11215, 11800
Tth111I GACNNNGTC 3 cut(s) 2912, 7732, 7856
Van91I CCANNNNNTGG 4 cut(s) 760, 1013, 2345, 6896
Vha464I CTTAAG 4 cut(s) 3065, 3598, 4208, 10627
VneI GTGCAC 5 cut(s) 2351, 6696, 8238, 9968, 11427
VpaK11BI GGWCC 8 cut(s) 1093, 3841, 6099, 7723, 9459, 10459, 11012, 12287
VspI ATTAAT 5 cut(s) 1416, 2919, 6306, 9510, 11663
XbaI TCTAGA 4 cut(s) 5599, 7336, 9593, 10535
XceI RCATGY 8 cut(s) 1250, 1477, 2104, 4399, 7203, 8624, 9724, 9925
XcmI CCANNNNNNNNNTGG 4 cut(s) 1029, 6819, 7844, 10972
XhoI CTCGAG 2 cut(s) 5115, 9798
XmaJI CCTAGG 3 cut(s) 3874, 7091, 8750
XmiI GTMKAC 7 cut(s) 1722, 2635, 5302, 5498, 6758, 8963, 11788
XmnI GAANNNNTTC 3 cut(s) 5763, 11076, 12044
ZrmI AGTACT 3 cut(s) 3625, 7434, 9684
Zsp2I ATGCAT 3 cut(s) 5803, 7043, 8914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.