Rmu_sc0002289.1_g000014

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002289.1
OG0005876
Physical Location & Seq
Reverse (-)
83741 .. 90466
6726 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1155 bp
atggctttatctcagtttctcggccataatccaatcttgcagccttgtcccaattcgctacgttcacatgggtcctcctcatatttcaactcaaagtcgagaaaagtcagagtcatggtttctgccaacagaaaacaagtagagatagtatacgatcctgatgaaaggataaacaagttggcagataaggtggaaaatgaggctccgccgctttcaaggctgtcactgttctcgccttgcaagattaatgttttcttgagaataactaacaaaagagaagatgggtttcatgacttggcatctctctttcatgtcataagccttggagatgtgattaagttctctttgtcaccctcaaaatccaaagatcgtttgtcgaccaatgtgtcaggggttccccttgatgataggaatttgattatcaaggcccttaatctctacaggaacaagactggtaccgacaatttcttttggattcatcttgataagaaagtgcctacaggagcaggacttggtggtggaagcagcaatgctgcaactgcgctatgggcagcaaatcagtttaatggttgtcttgccactgagaaggagctccaagaatggtcgagtgagattggttcagatgttcccttctttttctctcaaggtgcagcctattgtactggtcgtggtgaggttgttcaaaatattcctccacctgtaccctttgacattccgatggttcttataaagcctcagcaggcatgttcaacagctgaagtttacaagcacctgcagttggatacaactagtagtgttgatcctttaaaattgctggaaagaatctcagtgaatggaatatcgcaagatgtttgcatcaatgatttagaacctccagcatttcaagttcttccatctcttaaaagattgaagcagcgagtacttgctgctagccgtggacaatatgatgctgttttcatgtctggaagtggaagcactattgttgggattggttctccagatcctcctcagtttgtatatgatgacaacgaatacaaggatgtgtttttgtcagaggccaactttctcactcgcgaagaaaatcattggtacagagaaccttcatccagaagtgctagtggcccatcctctgatttttcccagtctattccttga

Protein Analysis

384

Amino Acids

42.37

Weight (kDa)

6.67

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GHMP_kinases_N PF00288 138 - 215 4.2e-14 GHMP kinases N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 728
AarI CACCTGC 1 cut(s) 780
AasI GACNNNNNNGTC 1 cut(s) 385
Acc36I ACCTGC 1 cut(s) 780
Acc65I GGTACC 1 cut(s) 455
AccB1I GGYRCC 1 cut(s) 455
AccI GTMKAC 2 cut(s) 150, 377
AccII CGCG 1 cut(s) 1074
AciI CCGC 2 cut(s) 206, 209
AclWI GGATC 3 cut(s) 149, 794, 995
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 1 cut(s) 412
AcuI CTGAAG 1 cut(s) 777
AfaI GTAC 5 cut(s) 457, 661, 702, 921, 1091
AfiI CCNNNNNNNGG 1 cut(s) 778
AgsI TTSAA 6 cut(s) 88, 216, 683, 750, 884, 910
AhlI ACTAGT 1 cut(s) 788
AluBI AGCT 2 cut(s) 592, 755
AluI AGCT 2 cut(s) 592, 755
Alw21I GWGCWC 1 cut(s) 594
AlwI GGATC 3 cut(s) 149, 794, 995
AoxI GGCC 4 cut(s) 22, 426, 1056, 1120
ApeKI GCWGC 7 cut(s) 40, 525, 533, 551, 650, 913, 926
ApoI RAATTY 1 cut(s) 412
AseI ATTAAT 1 cut(s) 246
Asp718I GGTACC 1 cut(s) 455
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 3 cut(s) 72, 427, 1121
AsuHPI GGTGA 2 cut(s) 342, 683
AsuNHI GCTAGC 1 cut(s) 929
AvaII GGWCC 1 cut(s) 72
BanI GGYRCC 1 cut(s) 455
BanII GRGCYC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 594
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 7 cut(s) 52, 520, 537, 563, 662, 913, 925
BccI CCATC 4 cut(s) 275, 712, 901, 1132
BceAI ACGGC 1 cut(s) 918
BciVI GTATCC 1 cut(s) 775
BcuI ACTAGT 1 cut(s) 788
BfaI CTAG 3 cut(s) 789, 930, 1116
BfmI CTRYAG 3 cut(s) 439, 498, 773
BfuAI ACCTGC 1 cut(s) 780
BfuI GTATCC 1 cut(s) 775
BisI GCNGC 8 cut(s) 41, 209, 526, 534, 552, 651, 914, 927
BlsI GCNGC 8 cut(s) 42, 210, 527, 535, 553, 652, 915, 928
BmcAI AGTACT 1 cut(s) 921
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 3 cut(s) 72, 427, 1121
BmiI GGNNCC 4 cut(s) 73, 204, 396, 457
BmrI ACTGGG 1 cut(s) 1135
BmsI GCATC 3 cut(s) 308, 864, 937
BmtI GCTAGC 1 cut(s) 933
BmuI ACTGGG 1 cut(s) 1135
BpmI CTGGAG 2 cut(s) 858, 981
Bpu10I CCTNAGC 1 cut(s) 735
BpuEI CTTGAG 2 cut(s) 277, 627
BsaJI CCNNGG 2 cut(s) 322, 934
Bsc4I CCNNNNNNNGG 1 cut(s) 778
Bse1I ACTGG 3 cut(s) 457, 667, 1141
Bse3DI GCAATG 1 cut(s) 535
BseDI CCNNGG 2 cut(s) 322, 934
BseGI GGATG 3 cut(s) 1045, 1103, 1124
BseLI CCNNNNNNNGG 1 cut(s) 778
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 5 cut(s) 26, 573, 749, 840, 1022
BseNI ACTGG 3 cut(s) 457, 667, 1141
BseRI GAGGAG 2 cut(s) 67, 996
BseXI GCAGC 7 cut(s) 52, 520, 537, 563, 662, 913, 925
BsgI GTGCAG 1 cut(s) 669
Bsh1236I CGCG 1 cut(s) 1074
BshFI GGCC 4 cut(s) 24, 428, 1058, 1122
BshNI GGYRCC 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 594
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 778
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 4 cut(s) 24, 428, 1058, 1122
Bsp1286I GDGCHC 1 cut(s) 594
Bsp143I GATC 4 cut(s) 154, 367, 799, 1000
Bsp68I TCGCGA 1 cut(s) 1074
BspACI CCGC 2 cut(s) 206, 209
BspANI GGCC 4 cut(s) 24, 428, 1058, 1122
BspCNI CTCAG 5 cut(s) 25, 574, 748, 839, 1021
BspFNI CGCG 1 cut(s) 1074
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 4 cut(s) 73, 204, 396, 457
BspMAI CTGCAG 1 cut(s) 777
BspMI ACCTGC 1 cut(s) 780
BspOI GCTAGC 1 cut(s) 933
BspPI GGATC 3 cut(s) 149, 794, 995
BspT107I GGYRCC 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 535
BsrI ACTGG 3 cut(s) 457, 667, 1141
BssECI CCNNGG 2 cut(s) 322, 934
BssMI GATC 4 cut(s) 154, 367, 799, 1000
BssNAI GTATAC 1 cut(s) 151
BssT1I CCWWGG 1 cut(s) 322
Bst1107I GTATAC 1 cut(s) 151
Bst4CI ACNGT 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 741, 931
BstDEI CTNAG 5 cut(s) 12, 582, 735, 826, 1008
BstDSI CCRYGG 1 cut(s) 934
BstF5I GGATG 3 cut(s) 1045, 1103, 1124
BstFNI CGCG 1 cut(s) 1074
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 4 cut(s) 157, 370, 802, 1003
BstMBI GATC 4 cut(s) 154, 367, 799, 1000
BstMWI GCNNNNNNNGC 3 cut(s) 217, 539, 548
BstNSI RCATGY 1 cut(s) 747
BstSFI CTRYAG 3 cut(s) 439, 498, 773
BstUI CGCG 1 cut(s) 1074
BstV1I GCAGC 7 cut(s) 52, 520, 537, 563, 662, 913, 925
BstX2I RGATCY 1 cut(s) 1000
BstYI RGATCY 1 cut(s) 1000
BstZ17I GTATAC 1 cut(s) 151
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 4 cut(s) 24, 428, 1058, 1122
BtgI CCRYGG 1 cut(s) 934
BtsCI GGATG 3 cut(s) 1045, 1103, 1124
BtsIMutI CAGTG 3 cut(s) 224, 579, 834
BtuMI TCGCGA 1 cut(s) 1074
BveI ACCTGC 1 cut(s) 780
Cac8I GCNNGC 2 cut(s) 741, 931
CciI TCATGA 1 cut(s) 289
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 3 cut(s) 72, 427, 1121
Csp6I GTAC 5 cut(s) 456, 660, 701, 920, 1090
CviAII CATG 6 cut(s) 68, 115, 290, 311, 744, 958
CviQI GTAC 5 cut(s) 456, 660, 701, 920, 1090
DdeI CTNAG 5 cut(s) 12, 582, 735, 826, 1008
DpnI GATC 4 cut(s) 156, 369, 801, 1002
DpnII GATC 4 cut(s) 154, 367, 799, 1000
DraI TTTAAA 1 cut(s) 807
DrdI GACNNNNNNGTC 1 cut(s) 385
DseDI GACNNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 22
EciI GGCGGA 1 cut(s) 195
Ecl136II GAGCTC 1 cut(s) 592
Eco130I CCWWGG 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 594
Eco47I GGWCC 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 592
Eco57I CTGAAG 1 cut(s) 777
EcoICRI GAGCTC 1 cut(s) 592
EcoO109I RGGNCCY 2 cut(s) 72, 427
EcoT14I CCWWGG 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 6 cut(s) 71, 118, 293, 314, 747, 961
FaqI GGGAC 1 cut(s) 33
FatI CATG 6 cut(s) 67, 114, 289, 310, 743, 957
FblI GTMKAC 2 cut(s) 150, 377
Fnu4HI GCNGC 8 cut(s) 41, 209, 526, 534, 552, 651, 914, 927
FokI GGATG 3 cut(s) 1052, 1090, 1111
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 8 cut(s) 41, 209, 526, 534, 552, 651, 914, 927
FspBI CTAG 3 cut(s) 789, 930, 1116
GlaI GCGC 1 cut(s) 543
GluI GCNGC 8 cut(s) 41, 209, 526, 534, 552, 651, 914, 927
GsuI CTGGAG 2 cut(s) 858, 981
HaeIII GGCC 4 cut(s) 24, 428, 1058, 1122
HhaI GCGC 1 cut(s) 544
Hin1II CATG 6 cut(s) 71, 118, 293, 314, 747, 961
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 3 cut(s) 111, 475, 822
HphI GGTGA 2 cut(s) 342, 683
Hpy166II GTNNAC 5 cut(s) 65, 151, 378, 763, 938
Hpy188I TCNGA 5 cut(s) 110, 622, 717, 1054, 1132
Hpy188III TCNNGA 9 cut(s) 99, 158, 256, 290, 482, 963, 998, 1073, 1107
Hpy8I GTNNAC 5 cut(s) 65, 151, 378, 763, 938
HpyAV CCTTC 3 cut(s) 580, 640, 1110
HpyCH4III ACNGT 1 cut(s) 228
HpyCH4IV ACGT 1 cut(s) 61
HpyCH4V TGCA 6 cut(s) 40, 240, 536, 650, 775, 855
HpyF10VI GCNNNNNNNGC 3 cut(s) 217, 539, 548
HpyF3I CTNAG 5 cut(s) 12, 582, 735, 826, 1008
HpySE526I ACGT 1 cut(s) 61
Hsp92II CATG 6 cut(s) 71, 118, 293, 314, 747, 961
HspAI GCGC 1 cut(s) 542
KpnI GGTACC 1 cut(s) 459
Kzo9I GATC 4 cut(s) 154, 367, 799, 1000
LmnI GCTCC 4 cut(s) 208, 503, 589, 597
Lsp1109I GCAGC 7 cut(s) 52, 520, 537, 563, 662, 913, 925
LweI GCATC 3 cut(s) 308, 864, 937
MaeI CTAG 3 cut(s) 789, 930, 1116
MaeII ACGT 1 cut(s) 61
MaeIII GTNAC 2 cut(s) 222, 348
MalI GATC 4 cut(s) 156, 369, 801, 1002
MboI GATC 4 cut(s) 154, 367, 799, 1000
MboII GAAGA 3 cut(s) 290, 881, 1088
MflI RGATCY 1 cut(s) 1000
MhlI GDGCHC 1 cut(s) 594
MluCI AATT 4 cut(s) 52, 412, 463, 809
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 759
MseI TTAA 6 cut(s) 246, 336, 432, 564, 806, 900
MslI CAYNNNNRTG 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 755
MvnI CGCG 1 cut(s) 1074
MwoI GCNNNNNNNGC 3 cut(s) 217, 539, 548
NdeII GATC 4 cut(s) 154, 367, 799, 1000
NheI GCTAGC 1 cut(s) 929
NlaIII CATG 6 cut(s) 71, 118, 293, 314, 747, 961
NlaIV GGNNCC 4 cut(s) 73, 204, 396, 457
NmuCI GTSAC 2 cut(s) 222, 348
NruI TCGCGA 1 cut(s) 1074
NspI RCATGY 1 cut(s) 747
PagI TCATGA 1 cut(s) 289
PaqCI CACCTGC 1 cut(s) 780
PfeI GAWTC 2 cut(s) 475, 822
PkrI GCNGC 8 cut(s) 42, 210, 527, 535, 553, 652, 915, 928
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PpuMI RGGWCCY 1 cut(s) 72
PshBI ATTAAT 1 cut(s) 246
PsiI TTATAA 1 cut(s) 728
Psp124BI GAGCTC 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 72
PspN4I GGNNCC 4 cut(s) 73, 204, 396, 457
PspPI GGNCC 3 cut(s) 72, 427, 1121
PspPPI RGGWCCY 1 cut(s) 72
PstI CTGCAG 1 cut(s) 777
PsuI RGATCY 1 cut(s) 1000
PvuII CAGCTG 1 cut(s) 755
RruI TCGCGA 1 cut(s) 1074
RsaI GTAC 5 cut(s) 457, 661, 702, 921, 1091
RsaNI GTAC 5 cut(s) 456, 660, 701, 920, 1090
RseI CAYNNNNRTG 1 cut(s) 716
SacI GAGCTC 1 cut(s) 594
SalI GTCGAC 1 cut(s) 376
SaqAI TTAA 6 cut(s) 246, 336, 432, 564, 806, 900
SatI GCNGC 8 cut(s) 41, 209, 526, 534, 552, 651, 914, 927
Sau3AI GATC 4 cut(s) 154, 367, 799, 1000
Sau96I GGNCC 3 cut(s) 72, 427, 1121
ScaI AGTACT 1 cut(s) 921
SchI GAGTC 1 cut(s) 120
SduI GDGCHC 1 cut(s) 594
SfaNI GCATC 3 cut(s) 308, 864, 937
SfcI CTRYAG 3 cut(s) 439, 498, 773
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 2 cut(s) 256, 642
SmoI CTYRAG 2 cut(s) 256, 642
SpeI ACTAGT 1 cut(s) 788
Sse9I AATT 4 cut(s) 52, 412, 463, 809
SsiI CCGC 2 cut(s) 206, 209
SspI AATATT 1 cut(s) 688
SspMI CTAG 3 cut(s) 789, 930, 1116
SstI GAGCTC 1 cut(s) 594
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 1 cut(s) 228
TaiI ACGT 1 cut(s) 64
TaqI TCGA 3 cut(s) 98, 377, 605
TasI AATT 4 cut(s) 52, 412, 463, 809
TatI WGTACW 2 cut(s) 659, 919
TauI GCSGC 1 cut(s) 211
TfiI GAWTC 2 cut(s) 475, 822
Tru1I TTAA 6 cut(s) 246, 336, 432, 564, 806, 900
Tru9I TTAA 6 cut(s) 246, 336, 432, 564, 806, 900
TscAI CASTG 3 cut(s) 231, 586, 834
TseFI GTSAC 2 cut(s) 222, 348
TseI GCWGC 7 cut(s) 40, 525, 533, 551, 650, 913, 926
Tsp45I GTSAC 2 cut(s) 222, 348
TspDTI ATGAA 6 cut(s) 177, 278, 299, 467, 946, 1092
TspRI CASTG 3 cut(s) 231, 586, 834
VpaK11BI GGWCC 1 cut(s) 72
VspI ATTAAT 1 cut(s) 246
XapI RAATTY 1 cut(s) 412
XceI RCATGY 1 cut(s) 747
XmiI GTMKAC 2 cut(s) 150, 377
XspI CTAG 3 cut(s) 789, 930, 1116
ZrmI AGTACT 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.