Rh5CG368000

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005876
Physical Location & Seq
Reverse (-)
45057754 .. 45063531
5778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG368000.1

Sequence Viewer

Length: 1224 bp
ATGCTGGGCCTGGCTGTCTGCACTGGGGGCATAGACTATCCTATGGCTTTATCTCAGTTTCTCGGCCATAATCCAATCTTGCAGCCTTGTCCCAATTTGTTCGAAAACAACCGCAGAGTGCATTCGTTACGTTCACATGGGTCTTCCTCATATTTCAACTCAAAGTCGAGAAATGTCAGAGTCATGGTTTCTGCCAACAGAAAACAAGTAGAGATAGTATACGATCCTGATGAAAGGATAAACAAGTTGGCAGATAAGGTGGACAATGAAGCTCCGCCGCTTTCAAGGCTATCACTGTTCTCGCCTTGCAAGATTAATGTTTTCTTGAGAATAACTAACAAAAGAGAAGATGGGTTTCATGACTTGGCATCTCTCTTTCATGTAATAAGCCTTGGAGATGTGATTAAGTTCTCTTTGTCACCCTCAAAATCCAAAGATCGTTTGTCGACCAATGTGTCAGGGGTTCCCCTTGATGATAGGAATTTGATTATCAAGGCCCTTAATCTCTACAGGAACAAGACTGGTAGCGACAATTTCTTTTGGATTCATCTTGATAAGAAAGTGCCTACAGGAGCAGGACTTGGTGGTGGAAGCAGCAATGCTGCAACTGCTCTATGGGCAGCAAATCAGTTTAATGGTTGTCTTGCCACTGAGAAGGAGCTCCAAGAATGGTCGAGTGAGATTGGTTCAGATGTTCCCTTCTTTTTCTCTCAAGGTGCAGCCTATTGTACTGGTCGTGGTGAGGTTGTTCAAAATATTCCTCCACCTGTACCCTTTGACATTCCGATGGTTCTTATAAAGCCTCAGCAGGCATGTTCAACAGCTGAAGTTTACAAGCGCCTGCAGTTGGATACAACTAGTAGTGTTGATCCTTTAAAATTACTGGAAAGAATCTCAGTGAATGGAATATCGCAAGATGTTTGCATCAATGATTTAGAACCTCCAGCATTTCAAGTTCTTCCATCTCTTAAAAGATTGAAGCAGCGAGTACTTGCTGCTAGCCGTGGACAATATGATGCTGTTTTCATGTCTGGAAGTGGAAGCACTATTGTTGGGATTGGTTCTCCAGATCCTCCTCAGTTTGTATATGATGACAATGAATACAAGGATGTGTTTTTGTCAGAGGCCAACTTTCTCACTCGCGAAGAAAATCATTGGTACAGAGAACCTCCATCCAGAAGTGCTAGTGGCCCATCCTCTGATTTTTCCCAGTCTATTCCTTGA

Protein Analysis

407

Amino Acids

44.92

Weight (kDa)

6.67

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GHMP_kinases_N PF00288 161 - 238 4.6e-14 GHMP kinases N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 797
AasI GACNNNNNNGTC 1 cut(s) 454
AccI GTMKAC 2 cut(s) 219, 446
AccII CGCG 1 cut(s) 1143
AciI CCGC 3 cut(s) 112, 275, 278
AclWI GGATC 3 cut(s) 218, 863, 1064
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 1 cut(s) 481
AcuI CTGAAG 1 cut(s) 846
AfaI GTAC 4 cut(s) 730, 771, 990, 1160
AfiI CCNNNNNNNGG 1 cut(s) 847
AgsI TTSAA 6 cut(s) 157, 285, 752, 819, 953, 979
AhlI ACTAGT 1 cut(s) 857
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 3 cut(s) 272, 661, 824
AluI AGCT 3 cut(s) 272, 661, 824
Alw21I GWGCWC 1 cut(s) 663
AlwI GGATC 3 cut(s) 218, 863, 1064
AoxI GGCC 5 cut(s) 7, 64, 495, 1125, 1189
ApeKI GCWGC 7 cut(s) 82, 594, 602, 620, 719, 982, 995
ApoI RAATTY 1 cut(s) 481
AseI ATTAAT 1 cut(s) 315
AspLEI GCGC 1 cut(s) 840
AspS9I GGNCC 3 cut(s) 7, 496, 1190
AsuHPI GGTGA 2 cut(s) 411, 752
AsuII TTCGAA 1 cut(s) 102
AsuNHI GCTAGC 1 cut(s) 998
BanII GRGCYC 1 cut(s) 663
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 663
BbvCI CCTCAGC 1 cut(s) 804
BbvI GCAGC 7 cut(s) 94, 589, 606, 632, 731, 982, 994
BccI CCATC 5 cut(s) 344, 781, 970, 1180, 1201
BceAI ACGGC 1 cut(s) 987
BciT130I CCWGG 1 cut(s) 11
BciVI GTATCC 1 cut(s) 844
BcuI ACTAGT 1 cut(s) 857
BfaI CTAG 3 cut(s) 858, 999, 1185
BfmI CTRYAG 3 cut(s) 508, 567, 842
BfoI RGCGCY 1 cut(s) 841
BfuI GTATCC 1 cut(s) 844
BisI GCNGC 8 cut(s) 83, 278, 595, 603, 621, 720, 983, 996
BlsI GCNGC 8 cut(s) 84, 279, 596, 604, 622, 721, 984, 997
BmcAI AGTACT 1 cut(s) 990
Bme1390I CCNGG 1 cut(s) 11
BmgT120I GGNCC 3 cut(s) 7, 496, 1190
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 1 cut(s) 11
BmrI ACTGGG 2 cut(s) 33, 1204
BmsI GCATC 3 cut(s) 377, 933, 1006
BmtI GCTAGC 1 cut(s) 1002
BmuI ACTGGG 2 cut(s) 33, 1204
BpiI GAAGAC 1 cut(s) 135
BpmI CTGGAG 2 cut(s) 927, 1050
Bpu10I CCTNAGC 1 cut(s) 804
Bpu14I TTCGAA 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 346, 696
BsaJI CCNNGG 2 cut(s) 391, 1003
Bsc4I CCNNNNNNNGG 1 cut(s) 847
Bse1I ACTGG 5 cut(s) 28, 526, 736, 888, 1210
Bse3DI GCAATG 1 cut(s) 604
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 2 cut(s) 391, 1003
BseGI GGATG 3 cut(s) 1114, 1172, 1193
BseLI CCNNNNNNNGG 1 cut(s) 847
BseMI GCAATG 1 cut(s) 604
BseMII CTCAG 5 cut(s) 68, 642, 818, 909, 1091
BseNI ACTGG 5 cut(s) 28, 526, 736, 888, 1210
BseRI GAGGAG 1 cut(s) 1065
BseXI GCAGC 7 cut(s) 94, 589, 606, 632, 731, 982, 994
BseYI CCCAGC 1 cut(s) 4
BsgI GTGCAG 2 cut(s) 4, 738
Bsh1236I CGCG 1 cut(s) 1143
BshFI GGCC 5 cut(s) 9, 66, 497, 1127, 1191
BsiHKAI GWGCWC 1 cut(s) 663
BslFI GGGAC 1 cut(s) 75
BslI CCNNNNNNNGG 1 cut(s) 847
BsmFI GGGAC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 121
BsnI GGCC 5 cut(s) 9, 66, 497, 1127, 1191
Bsp119I TTCGAA 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 663
Bsp143I GATC 4 cut(s) 223, 436, 868, 1069
Bsp68I TCGCGA 1 cut(s) 1143
BspACI CCGC 3 cut(s) 112, 275, 278
BspANI GGCC 5 cut(s) 9, 66, 497, 1127, 1191
BspCNI CTCAG 5 cut(s) 67, 643, 817, 908, 1090
BspFNI CGCG 1 cut(s) 1143
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 465
BspMAI CTGCAG 1 cut(s) 846
BspOI GCTAGC 1 cut(s) 1002
BspPI GGATC 3 cut(s) 218, 863, 1064
BspT104I TTCGAA 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 604
BsrI ACTGG 5 cut(s) 28, 526, 736, 888, 1210
BssECI CCNNGG 2 cut(s) 391, 1003
BssMI GATC 4 cut(s) 223, 436, 868, 1069
BssNAI GTATAC 1 cut(s) 220
BssT1I CCWWGG 1 cut(s) 391
Bst1107I GTATAC 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 1 cut(s) 297
BstBI TTCGAA 1 cut(s) 102
BstC8I GCNNGC 3 cut(s) 810, 842, 1000
BstDEI CTNAG 5 cut(s) 54, 651, 804, 895, 1077
BstDSI CCRYGG 1 cut(s) 1003
BstF5I GGATG 3 cut(s) 1114, 1172, 1193
BstFNI CGCG 1 cut(s) 1143
BstH2I RGCGCY 1 cut(s) 841
BstHHI GCGC 1 cut(s) 840
BstKTI GATC 4 cut(s) 226, 439, 871, 1072
BstMBI GATC 4 cut(s) 223, 436, 868, 1069
BstMWI GCNNNNNNNGC 4 cut(s) 27, 286, 608, 617
BstNI CCWGG 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 816
BstSCI CCNGG 1 cut(s) 9
BstSFI CTRYAG 3 cut(s) 508, 567, 842
BstUI CGCG 1 cut(s) 1143
BstV1I GCAGC 7 cut(s) 94, 589, 606, 632, 731, 982, 994
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 1069
BstZ17I GTATAC 1 cut(s) 220
BsuI GTATCC 1 cut(s) 844
BsuRI GGCC 5 cut(s) 9, 66, 497, 1127, 1191
BtgI CCRYGG 1 cut(s) 1003
BtsCI GGATG 3 cut(s) 1114, 1172, 1193
BtsIMutI CAGTG 4 cut(s) 21, 293, 648, 903
BtuMI TCGCGA 1 cut(s) 1143
Cac8I GCNNGC 3 cut(s) 810, 842, 1000
CciI TCATGA 1 cut(s) 358
CfoI GCGC 1 cut(s) 840
Cfr13I GGNCC 3 cut(s) 7, 496, 1190
Csp6I GTAC 4 cut(s) 729, 770, 989, 1159
CviAII CATG 6 cut(s) 137, 184, 359, 380, 813, 1027
CviQI GTAC 4 cut(s) 729, 770, 989, 1159
DdeI CTNAG 5 cut(s) 54, 651, 804, 895, 1077
DpnI GATC 4 cut(s) 225, 438, 870, 1071
DpnII GATC 4 cut(s) 223, 436, 868, 1069
DraI TTTAAA 1 cut(s) 876
DrdI GACNNNNNNGTC 1 cut(s) 454
DseDI GACNNNNNNGTC 1 cut(s) 454
EaeI YGGCCR 1 cut(s) 64
EciI GGCGGA 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 661
Eco130I CCWWGG 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 663
Eco53kI GAGCTC 1 cut(s) 661
Eco57I CTGAAG 1 cut(s) 846
EcoICRI GAGCTC 1 cut(s) 661
EcoO109I RGGNCCY 1 cut(s) 496
EcoRII CCWGG 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 6 cut(s) 140, 187, 362, 383, 816, 1030
FaqI GGGAC 1 cut(s) 75
FatI CATG 6 cut(s) 136, 183, 358, 379, 812, 1026
FblI GTMKAC 2 cut(s) 219, 446
Fnu4HI GCNGC 8 cut(s) 83, 278, 595, 603, 621, 720, 983, 996
FokI GGATG 3 cut(s) 1121, 1159, 1180
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 8 cut(s) 83, 278, 595, 603, 621, 720, 983, 996
FspBI CTAG 3 cut(s) 858, 999, 1185
GlaI GCGC 1 cut(s) 839
GluI GCNGC 8 cut(s) 83, 278, 595, 603, 621, 720, 983, 996
GsaI CCCAGC 1 cut(s) 8
GsuI CTGGAG 2 cut(s) 927, 1050
HaeII RGCGCY 1 cut(s) 841
HaeIII GGCC 5 cut(s) 9, 66, 497, 1127, 1191
HhaI GCGC 1 cut(s) 840
Hin1II CATG 6 cut(s) 140, 187, 362, 383, 816, 1030
Hin6I GCGC 1 cut(s) 838
HinP1I GCGC 1 cut(s) 838
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 3 cut(s) 180, 544, 891
HphI GGTGA 2 cut(s) 411, 752
Hpy166II GTNNAC 6 cut(s) 134, 220, 262, 447, 832, 1007
Hpy188I TCNGA 5 cut(s) 179, 691, 786, 1123, 1201
Hpy188III TCNNGA 9 cut(s) 168, 227, 325, 359, 551, 1032, 1067, 1142, 1176
Hpy8I GTNNAC 6 cut(s) 134, 220, 262, 447, 832, 1007
HpyAV CCTTC 2 cut(s) 649, 709
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 8 cut(s) 21, 82, 121, 309, 605, 719, 844, 924
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 286, 608, 617
HpyF3I CTNAG 5 cut(s) 54, 651, 804, 895, 1077
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 6 cut(s) 140, 187, 362, 383, 816, 1030
HspAI GCGC 1 cut(s) 838
Kzo9I GATC 4 cut(s) 223, 436, 868, 1069
LmnI GCTCC 4 cut(s) 277, 572, 658, 666
Lsp1109I GCAGC 7 cut(s) 94, 589, 606, 632, 731, 982, 994
LweI GCATC 3 cut(s) 377, 933, 1006
MaeI CTAG 3 cut(s) 858, 999, 1185
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 2 cut(s) 126, 417
MalI GATC 4 cut(s) 225, 438, 870, 1071
MboI GATC 4 cut(s) 223, 436, 868, 1069
MboII GAAGA 4 cut(s) 135, 359, 950, 1157
MflI RGATCY 1 cut(s) 1069
MhlI GDGCHC 1 cut(s) 663
MluCI AATT 4 cut(s) 94, 481, 532, 878
MlyI GAGTC 1 cut(s) 189
MmeI TCCRAC 1 cut(s) 828
MseI TTAA 6 cut(s) 315, 405, 501, 633, 875, 969
MslI CAYNNNNRTG 1 cut(s) 785
MspA1I CMGCKG 1 cut(s) 824
MspR9I CCNGG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 121
MvaI CCWGG 1 cut(s) 11
MvnI CGCG 1 cut(s) 1143
MwoI GCNNNNNNNGC 4 cut(s) 27, 286, 608, 617
NdeII GATC 4 cut(s) 223, 436, 868, 1069
NheI GCTAGC 1 cut(s) 998
NlaIII CATG 6 cut(s) 140, 187, 362, 383, 816, 1030
NlaIV GGNNCC 1 cut(s) 465
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 417
NruI TCGCGA 1 cut(s) 1143
NspI RCATGY 1 cut(s) 816
NspV TTCGAA 1 cut(s) 102
PagI TCATGA 1 cut(s) 358
PctI GAATGC 1 cut(s) 121
PfeI GAWTC 2 cut(s) 544, 891
PkrI GCNGC 8 cut(s) 84, 279, 596, 604, 622, 721, 984, 997
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PshBI ATTAAT 1 cut(s) 315
PsiI TTATAA 1 cut(s) 797
Psp124BI GAGCTC 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 9
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 3 cut(s) 7, 496, 1190
PstI CTGCAG 1 cut(s) 846
PsuI RGATCY 1 cut(s) 1069
PvuII CAGCTG 1 cut(s) 824
RruI TCGCGA 1 cut(s) 1143
RsaI GTAC 4 cut(s) 730, 771, 990, 1160
RsaNI GTAC 4 cut(s) 729, 770, 989, 1159
RseI CAYNNNNRTG 1 cut(s) 785
SacI GAGCTC 1 cut(s) 663
SalI GTCGAC 1 cut(s) 445
SaqAI TTAA 6 cut(s) 315, 405, 501, 633, 875, 969
SatI GCNGC 8 cut(s) 83, 278, 595, 603, 621, 720, 983, 996
Sau3AI GATC 4 cut(s) 223, 436, 868, 1069
Sau96I GGNCC 3 cut(s) 7, 496, 1190
ScaI AGTACT 1 cut(s) 990
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 663
SfaNI GCATC 3 cut(s) 377, 933, 1006
SfcI CTRYAG 3 cut(s) 508, 567, 842
SfuI TTCGAA 1 cut(s) 102
SmiMI CAYNNNNRTG 1 cut(s) 785
SmlI CTYRAG 2 cut(s) 325, 711
SmoI CTYRAG 2 cut(s) 325, 711
SpeI ACTAGT 1 cut(s) 857
Sse9I AATT 4 cut(s) 94, 481, 532, 878
SsiI CCGC 3 cut(s) 112, 275, 278
SspI AATATT 1 cut(s) 757
SspMI CTAG 3 cut(s) 858, 999, 1185
SstI GAGCTC 1 cut(s) 663
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 1 cut(s) 297
TaiI ACGT 1 cut(s) 133
TaqI TCGA 4 cut(s) 102, 167, 446, 674
TasI AATT 4 cut(s) 94, 481, 532, 878
TatI WGTACW 2 cut(s) 728, 988
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 2 cut(s) 544, 891
Tru1I TTAA 6 cut(s) 315, 405, 501, 633, 875, 969
Tru9I TTAA 6 cut(s) 315, 405, 501, 633, 875, 969
TscAI CASTG 4 cut(s) 28, 300, 655, 903
TseFI GTSAC 1 cut(s) 417
TseI GCWGC 7 cut(s) 82, 594, 602, 620, 719, 982, 995
Tsp45I GTSAC 1 cut(s) 417
TspDTI ATGAA 7 cut(s) 246, 282, 347, 368, 536, 1015, 1113
TspRI CASTG 4 cut(s) 28, 300, 655, 903
VspI ATTAAT 1 cut(s) 315
XapI RAATTY 1 cut(s) 481
XceI RCATGY 1 cut(s) 816
XmiI GTMKAC 2 cut(s) 219, 446
XspI CTAG 3 cut(s) 858, 999, 1185
ZrmI AGTACT 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.