Rroxscaffold_3G00235900

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005876
Physical Location & Seq
Reverse (-)
23104098 .. 23105056
959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235900.1

Sequence Viewer

Length: 672 bp
ATGGCAGCACCACTGTACCGAAGGGAATTGTTCTTGAAGTTCTCAAGTACTATAACTTTGAGGATTGGAGCATATGGGTCAAAACCTACCTGTTGGGGAAAGATCTCTTGGAAGTTGTGGAGTATTCATCTTGATAAGAAAGTGCCTACCGGAGCAGGACTTGGTGGTGGAAGCAGCAATGCCGCAACCGCACTATGGGCAGCAAATCGCTTTAATGGTTGTCTTGCCACTAAGAAGGAGCTCCAAGAATGGTCGAGTGAGATTGGTTCGGATGTTCCCTTCTTTTTCTCTCAAGGTGCAGCCTATTGTACCGGTCGTGGTGAGGTTGTTCAAAATATTCCTCCACCTGTACCCTTTGACTTTCCGATGGTTCTTATAAAGCCTCGGCAGGCATGTTCAACGAACCTCCAAGCATTTCAAGTTCTTCCATCTCTTAAAAGATTGAAGCACCGAGTACTTGCTGCTAGCCGTGGAGAATATGATGCTGTTTTCATGTCCGGAAATGGAAGCACTATTGTTGGGATTGGTTCTCCGGATCCTCCTCGGTTTGTATATGATGACAACGAATACAAGGATGTGTTTTTGTCGTGTGATCAATGGATTGACTTGGCGGACGATGGTGAATCGTTGATTGGGTCGTTAAGAAGACGCAACGGGATTAGAGGTGAGTAA

Protein Analysis

223

Amino Acids

24.5

Weight (kDa)

8.98

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GHMP_kinases_N PF00288 41 - 98 1.2e-11 GHMP kinases N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
AccIII TCCGGA 2 cut(s) 497, 532
AciI CCGC 3 cut(s) 183, 189, 611
AclWI GGATC 2 cut(s) 530, 543
AfaI GTAC 5 cut(s) 17, 49, 310, 351, 456
AfiI CCNNNNNNNGG 1 cut(s) 195
AgeI ACCGGT 1 cut(s) 311
AgsI TTSAA 5 cut(s) 37, 332, 399, 419, 445
AjuI GAANNNNNNNTTGG 4 cut(s) 91, 123, 615, 647
AluBI AGCT 1 cut(s) 241
AluI AGCT 1 cut(s) 241
Alw21I GWGCWC 1 cut(s) 243
AlwI GGATC 2 cut(s) 530, 543
Aor13HI TCCGGA 2 cut(s) 497, 532
ApeKI GCWGC 5 cut(s) 5, 174, 200, 299, 461
AsiGI ACCGGT 1 cut(s) 311
AsuHPI GGTGA 2 cut(s) 332, 632
AsuNHI GCTAGC 1 cut(s) 464
BaeI ACNNNNGTAYC 1 cut(s) 32
BamHI GGATCC 1 cut(s) 535
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 652
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 5 cut(s) 17, 186, 212, 311, 448
BccI CCATC 3 cut(s) 361, 436, 611
BceAI ACGGC 1 cut(s) 453
BclI TGATCA 1 cut(s) 592
BfaI CTAG 1 cut(s) 465
BglII AGATCT 1 cut(s) 102
BisI GCNGC 6 cut(s) 6, 175, 183, 201, 300, 462
BlsI GCNGC 6 cut(s) 7, 176, 184, 202, 301, 463
BmcAI AGTACT 2 cut(s) 49, 456
BmiI GGNNCC 1 cut(s) 537
BmsI GCATC 1 cut(s) 472
BmtI GCTAGC 1 cut(s) 468
BpiI GAAGAC 1 cut(s) 652
BpuEI CTTGAG 2 cut(s) 28, 276
BsaJI CCNNGG 3 cut(s) 383, 469, 542
BsaWI WCCGGW 4 cut(s) 149, 311, 497, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 195
Bse118I RCCGGY 1 cut(s) 311
Bse3DI GCAATG 1 cut(s) 184
BseAI TCCGGA 2 cut(s) 497, 532
BseDI CCNNGG 3 cut(s) 383, 469, 542
BseGI GGATG 2 cut(s) 277, 580
BseLI CCNNNNNNNGG 1 cut(s) 195
BseMI GCAATG 1 cut(s) 184
BseRI GAGGAG 1 cut(s) 531
BseXI GCAGC 5 cut(s) 17, 186, 212, 311, 448
BsgI GTGCAG 1 cut(s) 318
Bsh1285I CGRYCG 1 cut(s) 316
BshTI ACCGGT 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 316
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 4 cut(s) 150, 312, 498, 533
BslI CCNNNNNNNGG 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 243
Bsp13I TCCGGA 2 cut(s) 497, 532
Bsp143I GATC 3 cut(s) 102, 535, 592
BspACI CCGC 3 cut(s) 183, 189, 611
BspEI TCCGGA 2 cut(s) 497, 532
BspLI GGNNCC 1 cut(s) 537
BspOI GCTAGC 1 cut(s) 468
BspPI GGATC 2 cut(s) 530, 543
BsrDI GCAATG 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 311
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 3 cut(s) 383, 469, 542
BssMI GATC 3 cut(s) 102, 535, 592
Bst4CI ACNGT 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 390, 466
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 469
BstF5I GGATG 2 cut(s) 277, 580
BstKTI GATC 3 cut(s) 105, 538, 595
BstMBI GATC 3 cut(s) 102, 535, 592
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 2 cut(s) 188, 197
BstNSI RCATGY 1 cut(s) 396
BstV1I GCAGC 5 cut(s) 17, 186, 212, 311, 448
BstV2I GAAGAC 1 cut(s) 652
BstX2I RGATCY 2 cut(s) 102, 535
BstYI RGATCY 2 cut(s) 102, 535
BtgI CCRYGG 1 cut(s) 469
BtsCI GGATG 2 cut(s) 277, 580
BtsIMutI CAGTG 1 cut(s) 11
Cac8I GCNNGC 2 cut(s) 390, 466
Cfr10I RCCGGY 1 cut(s) 311
CseI GACGC 1 cut(s) 657
Csp6I GTAC 5 cut(s) 16, 48, 309, 350, 455
CspAI ACCGGT 1 cut(s) 311
CviAII CATG 2 cut(s) 393, 493
CviJI RGCY 4 cut(s) 241, 302, 382, 468
CviKI_1 RGCY 4 cut(s) 241, 302, 382, 468
CviQI GTAC 5 cut(s) 16, 48, 309, 350, 455
DdeI CTNAG 1 cut(s) 231
DpnI GATC 3 cut(s) 104, 537, 594
DpnII GATC 3 cut(s) 102, 535, 592
EciI GGCGGA 1 cut(s) 626
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 243
FaeI CATG 2 cut(s) 396, 496
FatI CATG 2 cut(s) 392, 492
FauNDI CATATG 1 cut(s) 73
FbaI TGATCA 1 cut(s) 592
Fnu4HI GCNGC 6 cut(s) 6, 175, 183, 201, 300, 462
FokI GGATG 2 cut(s) 284, 587
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 6 cut(s) 6, 175, 183, 201, 300, 462
FspBI CTAG 1 cut(s) 465
GluI GCNGC 6 cut(s) 6, 175, 183, 201, 300, 462
HapII CCGG 4 cut(s) 150, 312, 498, 533
HgaI GACGC 1 cut(s) 657
Hin1II CATG 2 cut(s) 396, 496
HinfI GANTC 1 cut(s) 623
HpaII CCGG 4 cut(s) 150, 312, 498, 533
HphI GGTGA 2 cut(s) 332, 632
Hpy188I TCNGA 2 cut(s) 271, 366
Hpy188III TCNNGA 4 cut(s) 34, 131, 498, 533
HpyAV CCTTC 3 cut(s) 15, 229, 289
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4V TGCA 1 cut(s) 299
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 197
HpyF3I CTNAG 1 cut(s) 231
Hsp92II CATG 2 cut(s) 396, 496
Kpn2I TCCGGA 2 cut(s) 497, 532
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 3 cut(s) 102, 535, 592
LmnI GCTCC 4 cut(s) 68, 152, 238, 246
LpnPI CCDG 8 cut(s) 103, 141, 163, 325, 360, 374, 511, 546
Lsp1109I GCAGC 5 cut(s) 17, 186, 212, 311, 448
LweI GCATC 1 cut(s) 472
MaeI CTAG 1 cut(s) 465
MalI GATC 3 cut(s) 104, 537, 594
MboI GATC 3 cut(s) 102, 535, 592
MboII GAAGA 2 cut(s) 416, 657
MflI RGATCY 2 cut(s) 102, 535
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 1 cut(s) 26
MnlI CCTC 8 cut(s) 54, 316, 351, 393, 416, 549, 552, 656
MroI TCCGGA 2 cut(s) 497, 532
MseI TTAA 3 cut(s) 213, 435, 641
MspI CCGG 4 cut(s) 150, 312, 498, 533
MwoI GCNNNNNNNGC 2 cut(s) 188, 197
NdeI CATATG 1 cut(s) 73
NdeII GATC 3 cut(s) 102, 535, 592
NheI GCTAGC 1 cut(s) 464
NlaIII CATG 2 cut(s) 396, 496
NlaIV GGNNCC 1 cut(s) 537
NmeAIII GCCGAG 1 cut(s) 364
NspI RCATGY 1 cut(s) 396
PfeI GAWTC 1 cut(s) 623
PinAI ACCGGT 1 cut(s) 311
PkrI GCNGC 6 cut(s) 7, 176, 184, 202, 301, 463
PsiI TTATAA 1 cut(s) 377
Psp124BI GAGCTC 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 537
PsuI RGATCY 2 cut(s) 102, 535
RsaI GTAC 5 cut(s) 17, 49, 310, 351, 456
RsaNI GTAC 5 cut(s) 16, 48, 309, 350, 455
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 3 cut(s) 213, 435, 641
SatI GCNGC 6 cut(s) 6, 175, 183, 201, 300, 462
Sau3AI GATC 3 cut(s) 102, 535, 592
ScaI AGTACT 2 cut(s) 49, 456
SduI GDGCHC 1 cut(s) 243
SetI ASST 8 cut(s) 88, 92, 243, 298, 327, 349, 408, 667
SfaNI GCATC 1 cut(s) 472
SmlI CTYRAG 2 cut(s) 43, 291
SmoI CTYRAG 2 cut(s) 43, 291
Sse9I AATT 1 cut(s) 26
SsiI CCGC 3 cut(s) 183, 189, 611
SspI AATATT 1 cut(s) 337
SspMI CTAG 1 cut(s) 465
SstI GAGCTC 1 cut(s) 243
TaaI ACNGT 1 cut(s) 15
TaqI TCGA 1 cut(s) 254
TasI AATT 1 cut(s) 26
TatI WGTACW 2 cut(s) 47, 454
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 1 cut(s) 623
Tru1I TTAA 3 cut(s) 213, 435, 641
Tru9I TTAA 3 cut(s) 213, 435, 641
TscAI CASTG 1 cut(s) 18
TseI GCWGC 5 cut(s) 5, 174, 200, 299, 461
TspDTI ATGAA 2 cut(s) 116, 481
TspRI CASTG 1 cut(s) 18
XceI RCATGY 1 cut(s) 396
XspI CTAG 1 cut(s) 465
ZrmI AGTACT 2 cut(s) 49, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.