Rorug05G0259000

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005876
Physical Location & Seq
Reverse (-)
29081023 .. 29085119
4097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0259000.1

Sequence Viewer

Length: 690 bp
ATGTCGTCTCTGAGGAATGCTGTTAGCAGACGTGCTCACAAGGAGCGATCTCAACCGGAATCGAGGAAAAAATTTGGGTTTCTTGAAAAGCACAAGGACTATGTTCAGCGTGCTAAGGTGTACCACAAAAAGGAGGAGACTTTACAGATACTGAAGCAAAAAGCAAGATTTCGAAACCCAGATGAGTTTTACTTCAAGATGATTAACACCCGAACTGTTGATGGAGTCCATAAAATAGAGGGTCAGGCTAACAAGTACACTCCAGAAGAGCTCTTGCTTATGAAGACCCAGGATATTGGATATGTACTGCAGAAAGTACAGAGTGAGAAAAAGAAAATTGAAAAGATGACTGCAACTCTGCATTCACTTGATAATCGGCCTTCAAGCAGACACGTATACTTTGCTGAAGATAGGGAAGAGGCTAAAGAGCTTCGGTCACGTAGTTCAAGAGGGGAAATATTGCTCGGTTCTGAGAATATTCCAGATCGTATTAAAAGGAAAACAGCGGCTTCATACAGGGAGCTTGAAGCAAGGAGAACGAGAGTCCAAGATCTGGAGAAAATATATATGGATATGGCAATGCAGAAGGAATTACAGAAAAAGGGCAAAAAACGCAAACTCCGTGAAGATGAGATGGTAAATCCAACATCCAAACCTGTATTCAAGTGGCGGGCAGAGCGAAAGCGTTGA

Protein Analysis

229

Amino Acids

27.39

Weight (kDa)

10.12

Isoelectric Point (pI)

69.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Utp11 PF03998 10 - 229 1.2e-60 Utp11 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 553
AccI GTMKAC 1 cut(s) 396
AciI CCGC 2 cut(s) 506, 670
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 2 cut(s) 173, 426
AfaI GTAC 4 cut(s) 122, 257, 306, 318
AfiI CCNNNNNNNGG 2 cut(s) 130, 553
AflIII ACRYGT 1 cut(s) 391
AgsI TTSAA 7 cut(s) 86, 196, 341, 384, 447, 527, 664
AjiI CACGTC 1 cut(s) 32
AjnI CCWGG 1 cut(s) 288
AjuI GAANNNNNNNTTGG 2 cut(s) 644, 676
AluBI AGCT 3 cut(s) 271, 430, 523
AluI AGCT 3 cut(s) 271, 430, 523
Alw21I GWGCWC 2 cut(s) 37, 273
Alw26I GTCTC 2 cut(s) 12, 131
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 1 cut(s) 377
ApoI RAATTY 1 cut(s) 71
AsuII TTCGAA 1 cut(s) 172
BanII GRGCYC 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 290
Bbv12I GWGCWC 2 cut(s) 37, 273
BccI CCATC 2 cut(s) 215, 628
BciT130I CCWGG 1 cut(s) 290
BcoDI GTCTC 2 cut(s) 12, 131
BfmI CTRYAG 1 cut(s) 308
BglII AGATCT 1 cut(s) 550
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
Bme1390I CCNGG 1 cut(s) 290
BmgBI CACGTC 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 290
BpiI GAAGAC 1 cut(s) 290
BpmI CTGGAG 2 cut(s) 246, 575
Bpu10I CCTNAGC 1 cut(s) 114
Bpu14I TTCGAA 1 cut(s) 172
BsaAI YACGTR 2 cut(s) 394, 440
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 553
Bse3DI GCAATG 1 cut(s) 585
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 1 cut(s) 647
BseLI CCNNNNNNNGG 2 cut(s) 130, 553
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 149
BshFI GGCC 1 cut(s) 379
BsiHKAI GWGCWC 2 cut(s) 37, 273
BsiSI CCGG 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 130, 553
BsmAI GTCTC 2 cut(s) 12, 131
BsmBI CGTCTC 1 cut(s) 12
BsmI GAATGC 2 cut(s) 22, 361
BsnI GGCC 1 cut(s) 379
Bsp119I TTCGAA 1 cut(s) 172
Bsp1286I GDGCHC 2 cut(s) 37, 273
Bsp143I GATC 3 cut(s) 47, 484, 550
BspACI CCGC 2 cut(s) 506, 670
BspANI GGCC 1 cut(s) 379
BspCNI CTCAG 1 cut(s) 463
BspMAI CTGCAG 1 cut(s) 312
BspQI GCTCTTC 1 cut(s) 261
BspT104I TTCGAA 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 585
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 3 cut(s) 47, 484, 550
BssNAI GTATAC 1 cut(s) 397
Bst1107I GTATAC 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 1 cut(s) 217
Bst6I CTCTTC 2 cut(s) 261, 411
BstBAI YACGTR 2 cut(s) 394, 440
BstBI TTCGAA 1 cut(s) 172
BstC8I GCNNGC 2 cut(s) 111, 672
BstDEI CTNAG 3 cut(s) 11, 114, 471
BstF5I GGATG 1 cut(s) 647
BstKTI GATC 3 cut(s) 50, 487, 553
BstMAI GTCTC 2 cut(s) 12, 131
BstMBI GATC 3 cut(s) 47, 484, 550
BstMWI GCNNNNNNNGC 2 cut(s) 612, 676
BstNI CCWGG 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 288
BstSFI CTRYAG 1 cut(s) 308
BstV2I GAAGAC 1 cut(s) 290
BstX2I RGATCY 1 cut(s) 550
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 1 cut(s) 550
BstZ17I GTATAC 1 cut(s) 397
BsuRI GGCC 1 cut(s) 379
BtrI CACGTC 1 cut(s) 32
BtsCI GGATG 1 cut(s) 647
Cac8I GCNNGC 2 cut(s) 111, 672
CaiI CAGNNNCTG 1 cut(s) 151
Csp6I GTAC 4 cut(s) 121, 256, 305, 317
CviJI RGCY 7 cut(s) 248, 271, 379, 422, 430, 509, 523
CviKI_1 RGCY 7 cut(s) 248, 271, 379, 422, 430, 509, 523
CviQI GTAC 4 cut(s) 121, 256, 305, 317
DdeI CTNAG 3 cut(s) 11, 114, 471
DpnI GATC 3 cut(s) 49, 486, 552
DpnII GATC 3 cut(s) 47, 484, 550
Eam1104I CTCTTC 2 cut(s) 261, 411
EarI CTCTTC 2 cut(s) 261, 411
Ecl136II GAGCTC 1 cut(s) 271
Eco24I GRGCYC 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 2 cut(s) 173, 426
EcoICRI GAGCTC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 273
Esp3I CGTCTC 1 cut(s) 12
FauI CCCGC 1 cut(s) 663
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 1 cut(s) 634
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 1 cut(s) 507
GluI GCNGC 1 cut(s) 507
GsuI CTGGAG 2 cut(s) 246, 575
HaeIII GGCC 1 cut(s) 379
HapII CCGG 1 cut(s) 56
HinfI GANTC 3 cut(s) 59, 225, 543
HpaII CCGG 1 cut(s) 56
Hpy166II GTNNAC 3 cut(s) 121, 258, 397
Hpy188I TCNGA 2 cut(s) 12, 472
Hpy188III TCNNGA 6 cut(s) 83, 196, 263, 447, 482, 554
Hpy8I GTNNAC 3 cut(s) 121, 258, 397
HpyAV CCTTC 2 cut(s) 390, 580
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 3 cut(s) 31, 393, 439
HpyCH4V TGCA 4 cut(s) 310, 353, 361, 583
HpyF10VI GCNNNNNNNGC 2 cut(s) 612, 676
HpyF3I CTNAG 3 cut(s) 11, 114, 471
HpySE526I ACGT 3 cut(s) 31, 393, 439
Kzo9I GATC 3 cut(s) 47, 484, 550
LguI GCTCTTC 1 cut(s) 261
LmnI GCTCC 2 cut(s) 43, 520
MaeII ACGT 3 cut(s) 31, 393, 439
MaeIII GTNAC 1 cut(s) 435
MalI GATC 3 cut(s) 49, 486, 552
MboI GATC 3 cut(s) 47, 484, 550
MboII GAAGA 5 cut(s) 278, 295, 419, 428, 638
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 2 cut(s) 37, 273
MluCI AATT 3 cut(s) 71, 336, 590
MlyI GAGTC 2 cut(s) 234, 552
MmeI TCCRAC 1 cut(s) 668
MnlI CCTC 6 cut(s) 6, 57, 127, 232, 412, 443
MseI TTAA 2 cut(s) 204, 492
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 290
Mva1269I GAATGC 2 cut(s) 22, 361
MvaI CCWGG 1 cut(s) 290
MwoI GCNNNNNNNGC 2 cut(s) 612, 676
NdeII GATC 3 cut(s) 47, 484, 550
NmuCI GTSAC 1 cut(s) 435
NspV TTCGAA 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 619
PctI GAATGC 2 cut(s) 22, 361
PfeI GAWTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 553
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 2 cut(s) 233, 551
PpsI GAGTC 2 cut(s) 233, 551
Ppu21I YACGTR 2 cut(s) 394, 440
Psp124BI GAGCTC 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 288
PstI CTGCAG 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 550
RsaI GTAC 4 cut(s) 122, 257, 306, 318
RsaNI GTAC 4 cut(s) 121, 256, 305, 317
SacI GAGCTC 1 cut(s) 273
SapI GCTCTTC 1 cut(s) 261
SaqAI TTAA 2 cut(s) 204, 492
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 3 cut(s) 47, 484, 550
SchI GAGTC 2 cut(s) 234, 552
ScrFI CCNGG 1 cut(s) 290
SduI GDGCHC 2 cut(s) 37, 273
SetI ASST 8 cut(s) 34, 120, 273, 396, 432, 442, 525, 658
SfcI CTRYAG 1 cut(s) 308
SfuI TTCGAA 1 cut(s) 172
Sse9I AATT 3 cut(s) 71, 336, 590
SsiI CCGC 2 cut(s) 506, 670
SspI AATATT 2 cut(s) 459, 478
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 1 cut(s) 288
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 3 cut(s) 34, 396, 442
TaqI TCGA 2 cut(s) 62, 172
TaqII GACCGA 1 cut(s) 423
TasI AATT 3 cut(s) 71, 336, 590
TatI WGTACW 3 cut(s) 255, 304, 316
TauI GCSGC 1 cut(s) 509
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 2 cut(s) 204, 492
Tru9I TTAA 2 cut(s) 204, 492
TseFI GTSAC 1 cut(s) 435
Tsp45I GTSAC 1 cut(s) 435
TspDTI ATGAA 2 cut(s) 296, 501
TspGWI ACGGA 1 cut(s) 611
Van91I CCANNNNNTGG 1 cut(s) 553
XapI RAATTY 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.