Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0002558.1

Orthogroup ID

OG0010633

Physical Location & Seq

Genomic Coordinates Reverse (-)

28164 .. 30217

Gene Structure 2054 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1407 bp

atgcgatcgtctattcctccatatgagaccaaggttcccatcaaagttcgcattcagggtcaagattcggtatacacattcgtcaagagcattgtgtatcatcttgtacaaccagcttcaaaagcttctagttccagttcaatggaatgggttgttaaagtccgagacattgaacccgaaaatcgttgtcttttgttacatccactttccacctcgttgcctttagagccgaattcagagtcttttccagatgaattaaacgtcttggaatttcaggtatcggagttagcaagatgttatagtctgcgtaaatccaattccaatgaggccgaacacaaggtggtcgtatcctctgatcctcattggcatgcgcttatgtcttacactgtatccattgattcatccttgaagacgacacaggtcgcgtatccaacaacttgttctcgcagtaagaaattggtggagtcattgggggatctacttttagttgagaaacgtgcgaggcctttgaggcggagatgtcctcataatccaactcatcatggatgcaaggtttacaagttgaacgaacccgagaggcaatgggttgaaatgaagagcttgagtaaagatcgaagtttgtttgtgggtgactacaccagcttctctgcttctactcgagatattcccagatgcttggggaatcacatttttttctcatcagatccatacttagggtttcatcgatccaagatcaagttgtacgtgaggcttctcatggatgcggaggtggtagtggtggaggtgatagtcagcgaacacgatgaggtaggggatctcagggagcatctacaggtggcagacttccttccccctccacattatctctttatgcataaaatgtctgtgatgttcgagataattccgttcagatggcataacgttctcgaaggtgatactatagatgtagttccagtgccgcaagtgctggaacaagaaaaagatgtaaaggtgttggtgacgtccttccgtgaaagtaaaaattactttgaagaggatctcgacacggaggaaataagttgtctcttacccatttcctgcttacactgttatactagggtgttccatgaactaatgatatctagtggtccacgattcactcacttgcagaacgagaggaggtgttatacgtacactaacatacgcagcggtgacacagtacaaattgtttgtatttcaggaataaaatttaagaagttgaaggtgaatgtgattgtggattataagggaactttggtggatatacatccttttaaaaaggtgaaagagctaaggaaggagctgaaggagaagaagttacatctcccgcgacggtattacttcatcctgctcgtccaaaatgctcaatatatatcttatggacgatga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009378 GO:0009987 GO:0016043 GO:0016462 GO:0016567 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0032392 GO:0032446 GO:0032508 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070035 GO:0070647 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901363 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF295 F-box F-box-like

Protein Analysis

468

Amino Acids

54.28

Weight (kDa)

8.59

Isoelectric Point (pI)

47.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_KIB1-4	PF03478	140 - 242	1.2e-18	KIB1-4 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010633)

Species	Orthologous Gene IDs
rosa_laevigata	RLG00000027029 RLG00000027032 RLG00000027034
rosa_multiflora	Rmu_sc0002558.1_g000001 Rmu_sc0002558.1_g000006 Rmu_sc0002558.1_g000007
rosa_roxburghii	Rroxscaffold_4G00286470 Rroxscaffold_4G00286480
rosa_rugosa	Rorug01G0357500
rosa_samantha	Rh1AG365900 Rh1AG366000 Rh1AG366100 Rh1AG366500 Rh1AG366600 Rh1CG342000 Rh1CG342100 Rh1CG342800 Rh1CG343000 Rh1CG344300
rosa_wichuraiana	Rw1G032240 Rw1G032260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1263
AatII	GACGTC	1 cut(s)	1004
AccI	GTMKAC	1 cut(s)	72
AccII	CGCG	2 cut(s)	425, 1348
AciI	CCGC	5 cut(s)	514, 764, 959, 1188, 1346
AclI	AACGTT	1 cut(s)	921
AclWI	GGATC	6 cut(s)	350, 483, 698, 720, 822, 1044
AcsI	RAATTY	3 cut(s)	232, 269, 1226
AcuI	CTGAAG	1 cut(s)	1343
AcyI	GRCGYC	1 cut(s)	1001
AdeI	CACNNNGTG	1 cut(s)	340
AfaI	GTAC	4 cut(s)	108, 743, 1172, 1200
AfiI	CCNNNNNNNGG	1 cut(s)	713
AgsI	TTSAA	8 cut(s)	120, 141, 173, 409, 565, 590, 1031, 1240
AjuI	GAANNNNNNNTTGG	2 cut(s)	424, 456
AluBI	AGCT	6 cut(s)	116, 125, 600, 642, 1309, 1321
AluI	AGCT	6 cut(s)	116, 125, 600, 642, 1309, 1321
Alw26I	GTCTC	3 cut(s)	20, 159, 1067
AlwI	GGATC	6 cut(s)	350, 483, 698, 720, 822, 1044
Ama87I	CYCGRG	2 cut(s)	572, 657
AoxI	GGCC	2 cut(s)	327, 503
ApeKI	GCWGC	1 cut(s)	1185
ApoI	RAATTY	3 cut(s)	232, 269, 1226
ArsI	GACNNNNNNTTYG	2 cut(s)	224, 256
AspLEI	GCGC	1 cut(s)	373
AspS9I	GGNCC	1 cut(s)	1127
AsuHPI	GGTGA	7 cut(s)	641, 796, 944, 1009, 1202, 1255, 1312
AvaI	CYCGRG	2 cut(s)	572, 657
AvaII	GGWCC	1 cut(s)	1127
BbsI	GAAGAC	1 cut(s)	416
BbvI	GCAGC	1 cut(s)	1197
BccI	CCATC	2 cut(s)	47, 906
BciVI	GTATCC	3 cut(s)	358, 400, 438
BcoDI	GTCTC	3 cut(s)	20, 159, 1067
BfaI	CTAG	3 cut(s)	129, 1095, 1122
BfmI	CTRYAG	2 cut(s)	830, 939
BfuI	GTATCC	3 cut(s)	358, 400, 438
BglI	GCCNNNNNGGC	1 cut(s)	511
BisI	GCNGC	2 cut(s)	959, 1186
BlsI	GCNGC	2 cut(s)	960, 1187
Bme18I	GGWCC	1 cut(s)	1127
BmeT110I	CYCGRG	2 cut(s)	572, 657
BmgT120I	GGNCC	1 cut(s)	1127
BmiI	GGNNCC	1 cut(s)	36
BmsI	GCATC	4 cut(s)	536, 662, 751, 835
BoxI	GACNNNNGTC	1 cut(s)	419
BpiI	GAAGAC	1 cut(s)	416
BplI	GAGNNNNNCTC	2 cut(s)	508, 540
Bpu10I	CCTNAGC	1 cut(s)	1310
BpuEI	CTTGAG	1 cut(s)	622
Bsa29I	ATCGAT	1 cut(s)	724
BsaAI	YACGTR	2 cut(s)	745, 1170
BsaBI	GATNNNNATC	1 cut(s)	1284
BsaHI	GRCGYC	1 cut(s)	1001
BsaI	GGTCTC	1 cut(s)	20
BsaJI	CCNNGG	1 cut(s)	30
BsaXI	ACNNNNNCTCC	2 cut(s)	1319, 1349
Bsc4I	CCNNNNNNNGG	1 cut(s)	713
Bse1I	ACTGG	2 cut(s)	135, 953
Bse3DI	GCAATG	1 cut(s)	587
Bse8I	GATNNNNATC	1 cut(s)	1284
BseCI	ATCGAT	1 cut(s)	724
BseDI	CCNNGG	1 cut(s)	30
BseGI	GGATG	6 cut(s)	199, 401, 551, 766, 1285, 1362
BseJI	GATNNNNATC	1 cut(s)	1284
BseLI	CCNNNNNNNGG	1 cut(s)	713
BseMI	GCAATG	1 cut(s)	587
BseMII	CTCAG	1 cut(s)	832
BseNI	ACTGG	2 cut(s)	135, 953
BseRI	GAGGAG	1 cut(s)	1171
BseXI	GCAGC	1 cut(s)	1197
Bsh1236I	CGCG	2 cut(s)	425, 1348
Bsh1285I	CGRYCG	1 cut(s)	8
BshFI	GGCC	2 cut(s)	329, 505
BshVI	ATCGAT	1 cut(s)	724
BsiEI	CGRYCG	1 cut(s)	8
BsiHKCI	CYCGRG	2 cut(s)	572, 657
BslI	CCNNNNNNNGG	1 cut(s)	713
BsmAI	GTCTC	3 cut(s)	20, 159, 1067
BsmI	GAATGC	1 cut(s)	51
BsnI	GGCC	2 cut(s)	329, 505
Bso31I	GGTCTC	1 cut(s)	20
BsoBI	CYCGRG	2 cut(s)	572, 657
Bsp1407I	TGTACA	1 cut(s)	106
Bsp143I	GATC	9 cut(s)	5, 355, 475, 610, 703, 725, 732, 814, 1036
BspACI	CCGC	5 cut(s)	514, 764, 959, 1188, 1346
BspANI	GGCC	2 cut(s)	329, 505
BspCNI	CTCAG	1 cut(s)	831
BspDI	ATCGAT	1 cut(s)	724
BspFNI	CGCG	2 cut(s)	425, 1348
BspLI	GGNNCC	1 cut(s)	36
BspPI	GGATC	6 cut(s)	350, 483, 698, 720, 822, 1044
BspQI	GCTCTTC	1 cut(s)	590
BspTNI	GGTCTC	1 cut(s)	20
BsrDI	GCAATG	1 cut(s)	587
BsrGI	TGTACA	1 cut(s)	106
BsrI	ACTGG	2 cut(s)	135, 953
BssECI	CCNNGG	1 cut(s)	30
BssMI	GATC	9 cut(s)	5, 355, 475, 610, 703, 725, 732, 814, 1036
BssNAI	GTATAC	1 cut(s)	73
BssNI	GRCGYC	1 cut(s)	1001
BssT1I	CCWWGG	1 cut(s)	30
Bst1107I	GTATAC	1 cut(s)	73
Bst4CI	ACNGT	4 cut(s)	388, 1088, 1198, 1353
Bst6I	CTCTTC	2 cut(s)	590, 1026
BstACI	GRCGYC	1 cut(s)	1001
BstAUI	TGTACA	1 cut(s)	106
BstBAI	YACGTR	2 cut(s)	745, 1170
BstC8I	GCNNGC	1 cut(s)	369
BstDEI	CTNAG	3 cut(s)	712, 818, 1310
BstF5I	GGATG	6 cut(s)	199, 401, 551, 766, 1285, 1362
BstFNI	CGCG	2 cut(s)	425, 1348
BstHHI	GCGC	1 cut(s)	373
BstKTI	GATC	9 cut(s)	8, 358, 478, 613, 706, 728, 735, 817, 1039
BstMAI	GTCTC	3 cut(s)	20, 159, 1067
BstMBI	GATC	9 cut(s)	5, 355, 475, 610, 703, 725, 732, 814, 1036
BstMCI	CGRYCG	1 cut(s)	8
BstMWI	GCNNNNNNNGC	4 cut(s)	122, 226, 511, 964
BstNSI	RCATGY	1 cut(s)	371
BstPAI	GACNNNNGTC	1 cut(s)	419
BstSFI	CTRYAG	2 cut(s)	830, 939
BstSNI	TACGTA	1 cut(s)	1170
BstUI	CGCG	2 cut(s)	425, 1348
BstV1I	GCAGC	1 cut(s)	1197
BstV2I	GAAGAC	1 cut(s)	416
BstX2I	RGATCY	4 cut(s)	475, 703, 814, 1036
BstXI	CCANNNNNNTGG	2 cut(s)	142, 676
BstYI	RGATCY	4 cut(s)	475, 703, 814, 1036
BstZ17I	GTATAC	1 cut(s)	73
Bsu15I	ATCGAT	1 cut(s)	724
BsuI	GTATCC	3 cut(s)	358, 400, 438
BsuRI	GGCC	2 cut(s)	329, 505
BsuTUI	ATCGAT	1 cut(s)	724
BtsCI	GGATG	6 cut(s)	199, 401, 551, 766, 1285, 1362
BtsIMutI	CAGTG	3 cut(s)	384, 960, 1084
Cac8I	GCNNGC	1 cut(s)	369
CfoI	GCGC	1 cut(s)	373
Cfr13I	GGNCC	1 cut(s)	1127
ClaI	ATCGAT	1 cut(s)	724
Csp6I	GTAC	4 cut(s)	107, 742, 1171, 1199
CviAII	CATG	4 cut(s)	368, 542, 757, 1106
CviQI	GTAC	4 cut(s)	107, 742, 1171, 1199
DdeI	CTNAG	3 cut(s)	712, 818, 1310
DpnI	GATC	9 cut(s)	7, 357, 477, 612, 705, 727, 734, 816, 1038
DpnII	GATC	9 cut(s)	5, 355, 475, 610, 703, 725, 732, 814, 1036
DraI	TTTAAA	1 cut(s)	1294
DraIII	CACNNNGTG	1 cut(s)	340
Eam1104I	CTCTTC	2 cut(s)	590, 1026
EarI	CTCTTC	2 cut(s)	590, 1026
EciI	GGCGGA	1 cut(s)	529
Eco105I	TACGTA	1 cut(s)	1170
Eco130I	CCWWGG	1 cut(s)	30
Eco147I	AGGCCT	1 cut(s)	505
Eco31I	GGTCTC	1 cut(s)	20
Eco32I	GATATC	1 cut(s)	1119
Eco47I	GGWCC	1 cut(s)	1127
Eco57I	CTGAAG	1 cut(s)	1343
Eco88I	CYCGRG	2 cut(s)	572, 657
EcoRI	GAATTC	1 cut(s)	232
EcoRV	GATATC	1 cut(s)	1119
EcoT14I	CCWWGG	1 cut(s)	30
EcoT22I	ATGCAT	1 cut(s)	876
ErhI	CCWWGG	1 cut(s)	30
FaeI	CATG	4 cut(s)	371, 545, 760, 1109
FatI	CATG	4 cut(s)	367, 541, 756, 1105
FauI	CCCGC	1 cut(s)	1353
FauNDI	CATATG	1 cut(s)	22
FblI	GTMKAC	1 cut(s)	72
Fnu4HI	GCNGC	2 cut(s)	959, 1186
FokI	GGATG	6 cut(s)	186, 388, 558, 773, 1272, 1349
Fsp4HI	GCNGC	2 cut(s)	959, 1186
FspBI	CTAG	3 cut(s)	129, 1095, 1122
GlaI	GCGC	1 cut(s)	372
GluI	GCNGC	2 cut(s)	959, 1186
HaeIII	GGCC	2 cut(s)	329, 505
HhaI	GCGC	1 cut(s)	373
Hin1I	GRCGYC	1 cut(s)	1001
Hin1II	CATG	4 cut(s)	371, 545, 760, 1109
Hin6I	GCGC	1 cut(s)	371
HinP1I	GCGC	1 cut(s)	371
HindIII	AAGCTT	1 cut(s)	123
HinfI	GANTC	6 cut(s)	65, 239, 398, 464, 682, 1134
HphI	GGTGA	7 cut(s)	641, 796, 944, 1009, 1202, 1255, 1312
Hpy166II	GTNNAC	4 cut(s)	73, 556, 1130, 1173
Hpy188I	TCNGA	6 cut(s)	164, 238, 283, 355, 703, 911
Hpy188III	TCNNGA	8 cut(s)	62, 85, 248, 659, 895, 926, 1040, 1218
Hpy8I	GTNNAC	4 cut(s)	73, 556, 1130, 1173
Hpy99I	CGWCG	1 cut(s)	1353
HpyAV	CCTTC	6 cut(s)	857, 923, 1015, 1234, 1309, 1318
HpyCH4III	ACNGT	4 cut(s)	388, 1088, 1198, 1353
HpyCH4IV	ACGT	6 cut(s)	261, 496, 744, 921, 1001, 1169
HpyCH4V	TGCA	3 cut(s)	549, 874, 1147
HpyF10VI	GCNNNNNNNGC	4 cut(s)	122, 226, 511, 964
HpyF3I	CTNAG	3 cut(s)	712, 818, 1310
HpySE526I	ACGT	6 cut(s)	261, 496, 744, 921, 1001, 1169
Hsp92I	GRCGYC	1 cut(s)	1001
Hsp92II	CATG	4 cut(s)	371, 545, 760, 1109
HspAI	GCGC	1 cut(s)	371
Kzo9I	GATC	9 cut(s)	5, 355, 475, 610, 703, 725, 732, 814, 1036
LguI	GCTCTTC	1 cut(s)	590
LmnI	GCTCC	2 cut(s)	823, 1318
Lsp1109I	GCAGC	1 cut(s)	1197
LweI	GCATC	4 cut(s)	536, 662, 751, 835
MaeI	CTAG	3 cut(s)	129, 1095, 1122
MaeII	ACGT	6 cut(s)	261, 496, 744, 921, 1001, 1169
MaeIII	GTNAC	5 cut(s)	195, 629, 997, 1190, 1335
MalI	GATC	9 cut(s)	7, 357, 477, 612, 705, 727, 734, 816, 1038
MboI	GATC	9 cut(s)	5, 355, 475, 610, 703, 725, 732, 814, 1036
MboII	GAAGA	4 cut(s)	421, 607, 1043, 1342
MflI	RGATCY	4 cut(s)	475, 703, 814, 1036
MluCI	AATT	9 cut(s)	232, 254, 269, 316, 455, 900, 1021, 1203, 1226
MlyI	GAGTC	2 cut(s)	248, 473
MmeI	TCCRAC	2 cut(s)	455, 557
Mph1103I	ATGCAT	1 cut(s)	876
MseI	TTAA	4 cut(s)	156, 257, 1230, 1293
MslI	CAYNNNNRTG	1 cut(s)	366
MspA1I	CMGCKG	1 cut(s)	1188
Mva1269I	GAATGC	1 cut(s)	51
MvnI	CGCG	2 cut(s)	425, 1348
MwoI	GCNNNNNNNGC	4 cut(s)	122, 226, 511, 964
NdeI	CATATG	1 cut(s)	22
NdeII	GATC	9 cut(s)	5, 355, 475, 610, 703, 725, 732, 814, 1036
NlaIII	CATG	4 cut(s)	371, 545, 760, 1109
NlaIV	GGNNCC	1 cut(s)	36
NmuCI	GTSAC	3 cut(s)	629, 997, 1190
NsiI	ATGCAT	1 cut(s)	876
NspI	RCATGY	1 cut(s)	371
PaeI	GCATGC	1 cut(s)	371
PaeR7I	CTCGAG	1 cut(s)	657
PceI	AGGCCT	1 cut(s)	505
PciSI	GCTCTTC	1 cut(s)	590
PctI	GAATGC	1 cut(s)	51
PfeI	GAWTC	4 cut(s)	65, 398, 682, 1134
PkrI	GCNGC	2 cut(s)	960, 1187
Ple19I	CGATCG	1 cut(s)	8
PleI	GAGTC	2 cut(s)	247, 472
PpsI	GAGTC	2 cut(s)	247, 472
Ppu21I	YACGTR	2 cut(s)	745, 1170
PshAI	GACNNNNGTC	1 cut(s)	419
PsiI	TTATAA	1 cut(s)	1263
Psp1406I	AACGTT	1 cut(s)	921
PspN4I	GGNNCC	1 cut(s)	36
PspPI	GGNCC	1 cut(s)	1127
PsuI	RGATCY	4 cut(s)	475, 703, 814, 1036
PvuI	CGATCG	1 cut(s)	8
RsaI	GTAC	4 cut(s)	108, 743, 1172, 1200
RsaNI	GTAC	4 cut(s)	107, 742, 1171, 1199
RseI	CAYNNNNRTG	1 cut(s)	366
SapI	GCTCTTC	1 cut(s)	590
SaqAI	TTAA	4 cut(s)	156, 257, 1230, 1293
SatI	GCNGC	2 cut(s)	959, 1186
Sau3AI	GATC	9 cut(s)	5, 355, 475, 610, 703, 725, 732, 814, 1036
Sau96I	GGNCC	1 cut(s)	1127
SchI	GAGTC	2 cut(s)	248, 473
SfaNI	GCATC	4 cut(s)	536, 662, 751, 835
SfcI	CTRYAG	2 cut(s)	830, 939
Sfr274I	CTCGAG	1 cut(s)	657
SinI	GGWCC	1 cut(s)	1127
SlaI	CTCGAG	1 cut(s)	657
SmiMI	CAYNNNNRTG	1 cut(s)	366
SmlI	CTYRAG	2 cut(s)	601, 657
SmoI	CTYRAG	2 cut(s)	601, 657
SnaBI	TACGTA	1 cut(s)	1170
SphI	GCATGC	1 cut(s)	371
Sse9I	AATT	9 cut(s)	232, 254, 269, 316, 455, 900, 1021, 1203, 1226
SseBI	AGGCCT	1 cut(s)	505
SsiI	CCGC	5 cut(s)	514, 764, 959, 1188, 1346
SspMI	CTAG	3 cut(s)	129, 1095, 1122
StuI	AGGCCT	1 cut(s)	505
StyI	CCWWGG	1 cut(s)	30
TaaI	ACNGT	4 cut(s)	388, 1088, 1198, 1353
TaiI	ACGT	6 cut(s)	264, 499, 747, 924, 1004, 1172
TaqI	TCGA	6 cut(s)	613, 658, 724, 894, 927, 1041
TasI	AATT	9 cut(s)	232, 254, 269, 316, 455, 900, 1021, 1203, 1226
TatI	WGTACW	2 cut(s)	106, 1198
TauI	GCSGC	1 cut(s)	961
TfiI	GAWTC	4 cut(s)	65, 398, 682, 1134
Tru1I	TTAA	4 cut(s)	156, 257, 1230, 1293
Tru9I	TTAA	4 cut(s)	156, 257, 1230, 1293
TscAI	CASTG	3 cut(s)	391, 960, 1091
TseFI	GTSAC	3 cut(s)	629, 997, 1190
TseI	GCWGC	1 cut(s)	1185
Tsp45I	GTSAC	3 cut(s)	629, 997, 1190
TspDTI	ATGAA	6 cut(s)	267, 390, 608, 710, 1122, 1351
TspGWI	ACGGA	3 cut(s)	894, 998, 1061
TspRI	CASTG	3 cut(s)	391, 960, 1091
VpaK11BI	GGWCC	1 cut(s)	1127
XapI	RAATTY	3 cut(s)	232, 269, 1226
XceI	RCATGY	1 cut(s)	371
XhoI	CTCGAG	1 cut(s)	657
XmiI	GTMKAC	1 cut(s)	72
XspI	CTAG	3 cut(s)	129, 1095, 1122
ZraI	GACGTC	1 cut(s)	1002
Zsp2I	ATGCAT	1 cut(s)	876

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.