Rmu_sc0002785.1_g000008

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002785.1
OG0006285
Physical Location & Seq
Reverse (-)
35016 .. 39578
4563 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1545 bp
atgctcacaaggctccacaatctcaagctccattcaactgcaaaaacccacatcaatgccctgcgttacttcttaaaccctctatgcaccacaaccgaggtcgccgagtcaccggaccttccaagctgggtaaagttcttcgacgccaaccacccaaaacacgcagattcttcaacttcaaaaacccacatcaatgccctgcgttacttcttaaaccctctatgcaccacaaccgaggtcgccgagtcaccggaccttccaagctgggtaaagttcttcgacaccaaccacccaaaacacgcagctttcgacgacgacgacttcgtcatcccttcactggccaattgggtcgaaacccacaacctaaaccaccccaccaaacctcccaagccctcaacttccgataccgacgacctcgaaagcatcagtagaatcttgaaaatccggtacccattgccggaaaatgcagctgaagcgttggaagggtacgattttgagctgtcgccgactttcgacgacgacgacttcgtcatcccttcactggccaattgggtcgaaacccacaacctaaaccaccccaccaaacctcccaagccctcaacttccgataccgacgacctcgaaagcatcagtagaatcttaaaagtccggtacccatcgccggaaaatgcagctgaatcgttggaaaggtataattttgagttatcaaacggtttggtgcaacgggttttgatgaggtttagcaatgattgggtcccggcgttggggtttttcacttgggcaaaacgtcgaacaggttataggcatcttcctgaggcctataactctatgaacaatgtattgggaaagtccaagaattttgaccttatgtgggattttgttgaggaaatggatcaaattggagatgggtatgtgacattggagacaatgactaaggtgtttaggagacttgcaaaggctggtaagcaccaagaggcaatcgaggcgtttcggggcatggagcgattcggattgagcaaagatgttgtggcattgaacactctactgaatgcattggtgaaggagagaagcgttgagcttgcccaagaagctttcttggagttcaaggaatctatacctgtgaattctcacagctttaatgttttgatacatggctggtgtaagaataggaagttggatattgctaggaacacaatggaggagatggagagacacgggtttggcccggatgttttctcttatacttgtttggttgaggcgtattgtagtgataaggattttcgcaaagttgatgaagttcttagtgagatgaagggcaaggcttgcgagccgaatgctgtgacatacactatcgtaatgcatgctttggggaaggcgagagagataaccaaggctttggaggtttatgagttgatgaaagggaatggggttgttcctgatgtttcgttttacagctctttgatttacattcttggtaaagccggcaggcttaaggatgttcaagaagtggcatatgaggattttgggagatccaaaaatctgtaa

Protein Analysis

514

Amino Acids

58.57

Weight (kDa)

5.68

Isoelectric Point (pI)

33.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 369 - 501 2.7e-11 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 375 - 422 1.3e-12 PPR repeat family
PPR_3 PF13812 399 - 458 2.2e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 409 - 458 1.1e-14 PPR repeat family
PPR PF01535 447 - 477 6.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 323, 527
Acc65I GGTACC 2 cut(s) 447, 651
AccB1I GGYRCC 2 cut(s) 447, 651
AclWI GGATC 2 cut(s) 900, 1524
AcoI YGGCCR 2 cut(s) 339, 543
AcsI RAATTY 2 cut(s) 856, 1123
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 3 cut(s) 449, 488, 653
AfiI CCNNNNNNNGG 7 cut(s) 337, 457, 541, 661, 763, 764, 871
AflII CTTAAG 1 cut(s) 1490
AgsI TTSAA 7 cut(s) 36, 174, 180, 439, 1036, 1105, 1502
AjuI GAANNNNNNNTTGG 4 cut(s) 824, 856, 1157, 1189
Alw26I GTCTC 3 cut(s) 917, 940, 1204
AlwI GGATC 2 cut(s) 900, 1524
AoxI GGCC 4 cut(s) 339, 543, 816, 1222
ApeKI GCWGC 3 cut(s) 302, 467, 671
ApoI RAATTY 2 cut(s) 856, 1123
ArsI GACNNNNNNTTYG 4 cut(s) 305, 337, 509, 541
Asp718I GGTACC 2 cut(s) 447, 651
AspS9I GGNCC 4 cut(s) 115, 253, 754, 1223
AsuC2I CCSGG 2 cut(s) 758, 1226
AsuHPI GGTGA 3 cut(s) 102, 240, 1069
AvaII GGWCC 3 cut(s) 115, 253, 754
AxyI CCTNAGG 1 cut(s) 813
BalI TGGCCA 2 cut(s) 341, 545
BanI GGYRCC 2 cut(s) 447, 651
BbvI GCAGC 3 cut(s) 314, 479, 683
BccI CCATC 3 cut(s) 664, 899, 1198
BcgI CGANNNNNNTGC 4 cut(s) 660, 694, 1316, 1350
BcnI CCSGG 2 cut(s) 758, 1226
BcoDI GTCTC 3 cut(s) 917, 940, 1204
BfaI CTAG 1 cut(s) 1185
BfrI CTTAAG 1 cut(s) 1490
BisI GCNGC 3 cut(s) 303, 468, 672
BlsI GCNGC 3 cut(s) 304, 469, 673
Bme1390I CCNGG 2 cut(s) 758, 1226
Bme18I GGWCC 3 cut(s) 115, 253, 754
BmgT120I GGNCC 4 cut(s) 115, 253, 754, 1223
BmiI GGNNCC 5 cut(s) 14, 449, 653, 755, 756
BmrFI CCNGG 2 cut(s) 758, 1226
BmsI GCATC 3 cut(s) 432, 636, 814
BpuEI CTTGAG 1 cut(s) 8
BpuMI CCSGG 2 cut(s) 758, 1226
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 96, 234, 1389
BsaWI WCCGGW 4 cut(s) 112, 250, 444, 648
BsaXI ACNNNNNCTCC 4 cut(s) 367, 397, 571, 601
Bsc4I CCNNNNNNNGG 7 cut(s) 337, 457, 541, 661, 763, 764, 871
Bse118I RCCGGY 1 cut(s) 1481
Bse1I ACTGG 2 cut(s) 342, 546
Bse21I CCTNAGG 1 cut(s) 813
Bse3DI GCAATG 2 cut(s) 452, 751
BseDI CCNNGG 3 cut(s) 96, 234, 1389
BseGI GGATG 4 cut(s) 327, 531, 1234, 1501
BseLI CCNNNNNNNGG 7 cut(s) 337, 457, 541, 661, 763, 764, 871
BseMI GCAATG 2 cut(s) 452, 751
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 2 cut(s) 342, 546
BseRI GAGGAG 1 cut(s) 1214
BseXI GCAGC 3 cut(s) 314, 479, 683
BseYI CCCAGC 2 cut(s) 126, 264
BshFI GGCC 4 cut(s) 341, 545, 818, 1224
BshNI GGYRCC 2 cut(s) 447, 651
BsiSI CCGG 9 cut(s) 113, 251, 445, 458, 649, 662, 758, 1226, 1482
BslFI GGGAC 1 cut(s) 740
BslI CCNNNNNNNGG 7 cut(s) 337, 457, 541, 661, 763, 764, 871
BsmAI GTCTC 3 cut(s) 917, 940, 1204
BsmFI GGGAC 1 cut(s) 740
BsmI GAATGC 2 cut(s) 1054, 1339
BsnI GGCC 4 cut(s) 341, 545, 818, 1224
Bsp143I GATC 2 cut(s) 892, 1529
BspANI GGCC 4 cut(s) 341, 545, 818, 1224
BspCNI CTCAG 1 cut(s) 805
BspLI GGNNCC 5 cut(s) 14, 449, 653, 755, 756
BspPI GGATC 2 cut(s) 900, 1524
BspT107I GGYRCC 2 cut(s) 447, 651
BspTI CTTAAG 1 cut(s) 1490
BsrDI GCAATG 2 cut(s) 452, 751
BsrFI RCCGGY 1 cut(s) 1481
BsrI ACTGG 2 cut(s) 342, 546
BssAI RCCGGY 1 cut(s) 1481
BssECI CCNNGG 3 cut(s) 96, 234, 1389
BssMI GATC 2 cut(s) 892, 1529
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 1389
Bst4CI ACNGT 1 cut(s) 713
BstACI GRCGYC 1 cut(s) 144
BstAFI CTTAAG 1 cut(s) 1490
BstAPI GCANNNNNTGC 1 cut(s) 1323
BstC8I GCNNGC 6 cut(s) 1080, 1324, 1328, 1362, 1483, 1487
BstDEI CTNAG 3 cut(s) 813, 933, 1301
BstF5I GGATG 4 cut(s) 327, 531, 1234, 1501
BstKTI GATC 2 cut(s) 895, 1532
BstMAI GTCTC 3 cut(s) 917, 940, 1204
BstMBI GATC 2 cut(s) 892, 1529
BstMWI GCNNNNNNNGC 5 cut(s) 10, 473, 983, 1088, 1323
BstNSI RCATGY 1 cut(s) 1364
BstSCI CCNGG 2 cut(s) 756, 1224
BstV1I GCAGC 3 cut(s) 314, 479, 683
BstX2I RGATCY 1 cut(s) 1529
BstXI CCANNNNNNTGG 1 cut(s) 1396
BstYI RGATCY 1 cut(s) 1529
Bsu36I CCTNAGG 1 cut(s) 813
BsuRI GGCC 4 cut(s) 341, 545, 818, 1224
BtgZI GCGATG 1 cut(s) 642
BtsCI GGATG 4 cut(s) 327, 531, 1234, 1501
BtsIMutI CAGTG 2 cut(s) 335, 539
Cac8I GCNNGC 6 cut(s) 1080, 1324, 1328, 1362, 1483, 1487
Cfr10I RCCGGY 1 cut(s) 1481
Cfr13I GGNCC 4 cut(s) 115, 253, 754, 1223
CseI GACGC 1 cut(s) 152
Csp6I GTAC 3 cut(s) 448, 487, 652
CviAII CATG 3 cut(s) 997, 1151, 1361
CviQI GTAC 3 cut(s) 448, 487, 652
DdeI CTNAG 3 cut(s) 813, 933, 1301
DpnI GATC 2 cut(s) 894, 1531
DpnII GATC 2 cut(s) 892, 1529
DrdI GACNNNNNNGTC 2 cut(s) 323, 527
DseDI GACNNNNNNGTC 2 cut(s) 323, 527
EaeI YGGCCR 2 cut(s) 339, 543
Eco130I CCWWGG 1 cut(s) 1389
Eco147I AGGCCT 1 cut(s) 818
Eco47I GGWCC 3 cut(s) 115, 253, 754
Eco57I CTGAAG 1 cut(s) 492
Eco81I CCTNAGG 1 cut(s) 813
EcoO109I RGGNCCY 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 1123
EcoT14I CCWWGG 1 cut(s) 1389
EcoT22I ATGCAT 2 cut(s) 1054, 1362
ErhI CCWWGG 1 cut(s) 1389
FaeI CATG 3 cut(s) 1000, 1154, 1364
FaqI GGGAC 1 cut(s) 740
FatI CATG 3 cut(s) 996, 1150, 1360
FauNDI CATATG 1 cut(s) 1513
Fnu4HI GCNGC 3 cut(s) 303, 468, 672
FokI GGATG 4 cut(s) 314, 518, 1241, 1508
Fsp4HI GCNGC 3 cut(s) 303, 468, 672
FspBI CTAG 1 cut(s) 1185
GluI GCNGC 3 cut(s) 303, 468, 672
GsaI CCCAGC 2 cut(s) 130, 268
HaeIII GGCC 4 cut(s) 341, 545, 818, 1224
HapII CCGG 9 cut(s) 113, 251, 445, 458, 649, 662, 758, 1226, 1482
HgaI GACGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 3 cut(s) 1000, 1154, 1364
HindIII AAGCTT 1 cut(s) 1089
HinfI GANTC 8 cut(s) 107, 167, 245, 432, 636, 677, 1005, 1109
HpaII CCGG 9 cut(s) 113, 251, 445, 458, 649, 662, 758, 1226, 1482
HphI GGTGA 3 cut(s) 102, 240, 1069
Hpy188I TCNGA 3 cut(s) 403, 607, 1010
Hpy188III TCNNGA 4 cut(s) 436, 812, 1436, 1502
HpyAV CCTTC 8 cut(s) 128, 266, 342, 476, 546, 1054, 1306, 1366
HpyCH4III ACNGT 1 cut(s) 713
HpyCH4IV ACGT 1 cut(s) 787
HpyCH4V TGCA 9 cut(s) 41, 87, 225, 467, 671, 721, 953, 1052, 1360
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 473, 983, 1088, 1323
HpyF3I CTNAG 3 cut(s) 813, 933, 1301
HpySE526I ACGT 1 cut(s) 787
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 3 cut(s) 1000, 1154, 1364
KflI GGGWCCC 1 cut(s) 754
KpnI GGTACC 2 cut(s) 451, 655
KroI GCCGGC 1 cut(s) 1481
KroNI GCCGGC 1 cut(s) 1483
Kzo9I GATC 2 cut(s) 892, 1529
LmnI GCTCC 3 cut(s) 18, 33, 1000
Lsp1109I GCAGC 3 cut(s) 314, 479, 683
LweI GCATC 3 cut(s) 432, 636, 814
MaeI CTAG 1 cut(s) 1185
MaeII ACGT 1 cut(s) 787
MaeIII GTNAC 6 cut(s) 65, 108, 203, 246, 913, 1339
MalI GATC 2 cut(s) 894, 1531
MboI GATC 2 cut(s) 892, 1529
MboII GAAGA 4 cut(s) 130, 162, 268, 800
MfeI CAATTG 2 cut(s) 343, 547
MflI RGATCY 1 cut(s) 1529
MlsI TGGCCA 2 cut(s) 341, 545
MluCI AATT 6 cut(s) 343, 547, 694, 856, 897, 1123
MluNI TGGCCA 2 cut(s) 341, 545
MlyI GAGTC 2 cut(s) 116, 254
MmeI TCCRAC 3 cut(s) 459, 663, 1155
Mox20I TGGCCA 2 cut(s) 341, 545
Mph1103I ATGCAT 2 cut(s) 1054, 1362
MroNI GCCGGC 1 cut(s) 1481
MscI TGGCCA 2 cut(s) 341, 545
MseI TTAA 5 cut(s) 74, 212, 641, 1137, 1491
MslI CAYNNNNRTG 2 cut(s) 54, 192
Msp20I TGGCCA 2 cut(s) 341, 545
MspA1I CMGCKG 2 cut(s) 470, 674
MspCI CTTAAG 1 cut(s) 1490
MspI CCGG 9 cut(s) 113, 251, 445, 458, 649, 662, 758, 1226, 1482
MspR9I CCNGG 2 cut(s) 758, 1226
MunI CAATTG 2 cut(s) 343, 547
Mva1269I GAATGC 2 cut(s) 1054, 1339
MwoI GCNNNNNNNGC 5 cut(s) 10, 473, 983, 1088, 1323
NaeI GCCGGC 1 cut(s) 1483
NciI CCSGG 2 cut(s) 758, 1226
NdeI CATATG 1 cut(s) 1513
NdeII GATC 2 cut(s) 892, 1529
NgoMIV GCCGGC 1 cut(s) 1481
NlaIII CATG 3 cut(s) 1000, 1154, 1364
NlaIV GGNNCC 5 cut(s) 14, 449, 653, 755, 756
NmeAIII GCCGAG 2 cut(s) 130, 268
NmuCI GTSAC 4 cut(s) 108, 246, 913, 1339
NsiI ATGCAT 2 cut(s) 1054, 1362
NspI RCATGY 1 cut(s) 1364
PaeI GCATGC 1 cut(s) 1364
PceI AGGCCT 1 cut(s) 818
PcsI WCGNNNNNNNCGW 5 cut(s) 306, 315, 321, 519, 525
PctI GAATGC 2 cut(s) 1054, 1339
PdiI GCCGGC 1 cut(s) 1483
PfeI GAWTC 6 cut(s) 167, 432, 636, 677, 1005, 1109
PflFI GACNNNGTC 2 cut(s) 323, 527
PkrI GCNGC 3 cut(s) 304, 469, 673
PleI GAGTC 2 cut(s) 115, 253
PpsI GAGTC 2 cut(s) 115, 253
PpuMI RGGWCCY 1 cut(s) 754
Psp5II RGGWCCY 1 cut(s) 754
PspFI CCCAGC 2 cut(s) 126, 264
PspN4I GGNNCC 5 cut(s) 14, 449, 653, 755, 756
PspPI GGNCC 4 cut(s) 115, 253, 754, 1223
PspPPI RGGWCCY 1 cut(s) 754
PsuI RGATCY 1 cut(s) 1529
PsyI GACNNNGTC 2 cut(s) 323, 527
PvuII CAGCTG 2 cut(s) 470, 674
RsaI GTAC 3 cut(s) 449, 488, 653
RsaNI GTAC 3 cut(s) 448, 487, 652
RseI CAYNNNNRTG 2 cut(s) 54, 192
SaqAI TTAA 5 cut(s) 74, 212, 641, 1137, 1491
SatI GCNGC 3 cut(s) 303, 468, 672
Sau3AI GATC 2 cut(s) 892, 1529
Sau96I GGNCC 4 cut(s) 115, 253, 754, 1223
SchI GAGTC 2 cut(s) 116, 254
ScrFI CCNGG 2 cut(s) 758, 1226
SfaNI GCATC 3 cut(s) 432, 636, 814
SinI GGWCC 3 cut(s) 115, 253, 754
SmiMI CAYNNNNRTG 2 cut(s) 54, 192
SmlI CTYRAG 2 cut(s) 23, 1490
SmoI CTYRAG 2 cut(s) 23, 1490
SphI GCATGC 1 cut(s) 1364
Sse9I AATT 6 cut(s) 343, 547, 694, 856, 897, 1123
SseBI AGGCCT 1 cut(s) 818
SspMI CTAG 1 cut(s) 1185
StuI AGGCCT 1 cut(s) 818
StyD4I CCNGG 2 cut(s) 756, 1224
StyI CCWWGG 1 cut(s) 1389
TaaI ACNGT 1 cut(s) 713
TaiI ACGT 1 cut(s) 790
TasI AATT 6 cut(s) 343, 547, 694, 856, 897, 1123
TfiI GAWTC 6 cut(s) 167, 432, 636, 677, 1005, 1109
Tru1I TTAA 5 cut(s) 74, 212, 641, 1137, 1491
Tru9I TTAA 5 cut(s) 74, 212, 641, 1137, 1491
TscAI CASTG 2 cut(s) 342, 546
TseFI GTSAC 4 cut(s) 108, 246, 913, 1339
TseI GCWGC 3 cut(s) 302, 467, 671
Tsp45I GTSAC 4 cut(s) 108, 246, 913, 1339
TspDTI ATGAA 4 cut(s) 845, 1308, 1325, 1430
TspRI CASTG 2 cut(s) 342, 546
Tth111I GACNNNGTC 2 cut(s) 323, 527
Vha464I CTTAAG 1 cut(s) 1490
VpaK11BI GGWCC 3 cut(s) 115, 253, 754
XapI RAATTY 2 cut(s) 856, 1123
XceI RCATGY 1 cut(s) 1364
XspI CTAG 1 cut(s) 1185
Zsp2I ATGCAT 2 cut(s) 1054, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.