Rmu_sc0003120.1_g000003

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003120.1
OG0003789
Physical Location & Seq
Reverse (-)
10443 .. 15757
5315 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2265 bp
atggccggcgatgagagatggtgcgtcgtcaccggaggccgaggcttcgccgctcgccatttggttctcatgctcatccgccaaaacgtcttccgcgtccgaatcgccgacctcggccccaccgtcgatctcgaaccttccgagtccaacggagtcctcgccgatgctttgaactccggccgcgcccagtacatttccatcgacctccgccgtaaggatcaagttcttcaagcatgtcaaggtgctgaggctgtgttccacatggcggctcccaattcgtcgatcaacaattaccagctccaccattcggtcaatgtggaaggaactaagaatgtgattgatgcttgtgttgagcagaaagtgaagaggctcatatacactagctctcctagtgttgtgtttgatggggtccatggcattattgatggggatgagtcattgccatatccacctaagcacaatgatacttattcggccactaaagcgcaaggagaagagttggtaatcaaggcaaacggtgttaaagggcttctaacctgctgcatacggcctagcagcattttcggacccggtgataggttacttgttccatctttaattgttgccgccagggcagggaaatccaagttcattatcggagatggcaataacatttatgacttcacgtatgttgaaaacgtggcccattcccatatatgtgcagaacgagctctagcgtctgaagggacagttgcagagaaagctgcaggacaggcatattttataaccaatatggaacctatcaagttttgggagtttatgtcacttattctagaaggtcttggctatgaaagaccaagaataaaaatccctgcagctgttatgatgccaattgcacatgtggtggagtggacatttaagctttttggtccatatgggatgaaggttccacagttgacgccttcaagaattagacttctcctagtgttgtgtttgatggggtccatggcattattgatggggatgactttgattttggattgtccttattggtttcagtacaatgatacttattcggccactaaagcacaaggagaagagttggtaatcaaggcaaacggtgttaaagggcttctaacctgctgcatacggcctagcagcattttcggacccggtgataggttacttgttccatctttaattgctgccgccagggcagggaaatccaagttcattatcggagatggcaataacatttatgacttcacgtatgttgaaaacgtggcccattcccatatatgtgcagaacgagctctagcgtctgaagggacagttgcagagaaagctgcaggacaggcatattttataaccaatatggaacctatcaagttttgggagtttatgtcacttattctagaaggtcttggctatgaaagaccaagaataaaaatccctgcagctgttatgatgccaattgcacatgtggtggagtggacatttaagctttttggtccatatgggatgaaggttccacagttgacgccttcaagaattagacttctctcttgcaccagatcttttaaatcttcaaaagcacaagatcgaattggttacacgcccatcatttcacttcaggagggtcttaagaggacaatcgagtcatacccacacttgagggctgaacatcgacccaaaagagaagggccatctaaagcttctgtgtatcttggaagtgggagggttgctgatgcattactgtggaaggacaaaaaacagacattcaaggcactattattttttattgcaatctactacaacttcattgcatctcaagctacagttattaccgtcatttcaaagagtctcatgttcacatcaattttcgtgttcatctctggcaatttaccagaaaagatattaggatataaactcgagaagcttcagtcatcacattttaacttatcagaagagatgtcacaacaaattgctgggtcggtggcctcatcatggaatatatctgtgaaaactttaaagtctcttgggaaaggaaatgactggatattgtttcttaaggtggttctatccttactggttcttagcttccttggagccatttcacttcagcactcatttattataggagtttccactgcctttctagccttctatgtgtacgaaaagcaggaagaaaaaatcgatgccatggtcctagaagctttctctcgtggatgcaaattgaagtccaatatgctccgaaaattctgtacatccaaaaagattgagtga

Protein Analysis

754

Amino Acids

83.46

Weight (kDa)

9.13

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 9 - 283 9.1e-77 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 9 - 237 5.4e-33 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 129 5.3e-07 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 10 - 232 6.4e-14 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 57 - 133 2.3e-08 NAD(P)H-binding
RmlD_sub_bind PF04321 67 - 282 3.7e-07 RmlD substrate binding domain
NAD_binding_4 PF07993 76 - 227 8.3e-13 Male sterility protein
3Beta_HSD PF01073 344 - 477 5.2e-38 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 346 - 431 3.7e-13 NAD dependent epimerase/dehydratase family
Reticulon PF02453 574 - 729 5.1e-31 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 764, 1346
AasI GACNNNNNNGTC 1 cut(s) 1636
Acc36I ACCTGC 2 cut(s) 545, 1127
AccBSI CCGCTC 1 cut(s) 53
AccII CGCG 2 cut(s) 96, 183
AciI CCGC 8 cut(s) 51, 79, 94, 181, 208, 266, 606, 1188
AclWI GGATC 1 cut(s) 225
AcoI YGGCCR 4 cut(s) 3, 178, 474, 1056
AcsI RAATTY 1 cut(s) 2237
AcuI CTGAAG 5 cut(s) 741, 1323, 1595, 1904, 2084
AcyI GRCGYC 2 cut(s) 938, 1520
AfaI GTAC 4 cut(s) 191, 1040, 2153, 2245
AfiI CCNNNNNNNGG 8 cut(s) 214, 265, 307, 576, 615, 1158, 1197, 1986
AflII CTTAAG 2 cut(s) 1622, 2048
AflIII ACRYGT 2 cut(s) 877, 1459
AjnI CCWGG 2 cut(s) 608, 1190
Alw21I GWGCWC 2 cut(s) 712, 1294
Alw26I GTCTC 2 cut(s) 1847, 2019
AlwI GGATC 1 cut(s) 225
Ama87I CYCGRG 1 cut(s) 1910
ApeKI GCWGC 9 cut(s) 540, 555, 743, 854, 1122, 1137, 1184, 1325, 1436
ApoI RAATTY 1 cut(s) 2237
ArsI GACNNNNNNTTYG 2 cut(s) 997, 1029
AspLEI GCGC 2 cut(s) 185, 487
AsuC2I CCSGG 2 cut(s) 570, 1152
AsuHPI GGTGA 3 cut(s) 22, 584, 1166
AvaI CYCGRG 1 cut(s) 1910
AvaII GGWCC 7 cut(s) 409, 566, 908, 981, 1148, 1490, 2185
BanII GRGCYC 2 cut(s) 712, 1294
BauI CACGAG 1 cut(s) 2202
BbsI GAAGAC 1 cut(s) 82
Bbv12I GWGCWC 2 cut(s) 712, 1294
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 9 cut(s) 527, 567, 730, 866, 1109, 1149, 1171, 1312, 1448
BceAI ACGGC 3 cut(s) 195, 563, 1145
BcgI CGANNNNNNTGC 4 cut(s) 544, 578, 1126, 1160
BciT130I CCWGG 2 cut(s) 610, 1192
BcnI CCSGG 2 cut(s) 570, 1152
BcoDI GTCTC 2 cut(s) 1847, 2019
BfmI CTRYAG 5 cut(s) 744, 852, 1326, 1434, 1815
BfrI CTTAAG 2 cut(s) 1622, 2048
BfuAI ACCTGC 2 cut(s) 545, 1127
BglI GCCNNNNNGGC 2 cut(s) 611, 1193
BglII AGATCT 1 cut(s) 1553
Bme1390I CCNGG 4 cut(s) 570, 610, 1152, 1192
Bme18I GGWCC 7 cut(s) 409, 566, 908, 981, 1148, 1490, 2185
BmeT110I CYCGRG 1 cut(s) 1910
BmrFI CCNGG 4 cut(s) 570, 610, 1152, 1192
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 8 cut(s) 154, 331, 855, 1437, 1717, 1814, 2167, 2198
BmuI ACTGGG 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 82
Bpu10I CCTNAGC 2 cut(s) 246, 453
BpuEI CTTGAG 2 cut(s) 1672, 1794
BpuMI CCSGG 2 cut(s) 570, 1152
Bsa29I ATCGAT 1 cut(s) 2175
BsaAI YACGTR 2 cut(s) 666, 1248
BsaHI GRCGYC 2 cut(s) 938, 1520
BsaJI CCNNGG 8 cut(s) 40, 112, 412, 609, 984, 1191, 2083, 2181
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 6 cut(s) 370, 400, 785, 815, 1367, 1397
Bsc4I CCNNNNNNNGG 8 cut(s) 214, 265, 307, 576, 615, 1158, 1197, 1986
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 3 cut(s) 187, 2039, 2073
Bse3DI GCAATG 2 cut(s) 437, 1800
BseBI CCWGG 2 cut(s) 610, 1192
BseCI ATCGAT 1 cut(s) 2175
BseDI CCNNGG 8 cut(s) 40, 112, 412, 609, 984, 1191, 2083, 2181
BseGI GGATG 7 cut(s) 75, 436, 924, 1008, 1506, 2213, 2246
BseLI CCNNNNNNNGG 8 cut(s) 214, 265, 307, 576, 615, 1158, 1197, 1986
BseMI GCAATG 2 cut(s) 437, 1800
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 187, 2039, 2073
BseX3I CGGCCG 1 cut(s) 178
BseXI GCAGC 9 cut(s) 527, 567, 730, 866, 1109, 1149, 1171, 1312, 1448
BseYI CCCAGC 1 cut(s) 1967
BsgI GTGCAG 2 cut(s) 720, 1302
Bsh1236I CGCG 2 cut(s) 96, 183
Bsh1285I CGRYCG 1 cut(s) 181
BshVI ATCGAT 1 cut(s) 2175
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 2 cut(s) 712, 1294
BsiHKCI CYCGRG 1 cut(s) 1910
BsiSI CCGG 5 cut(s) 6, 33, 177, 570, 1152
BslFI GGGAC 2 cut(s) 739, 1321
BslI CCNNNNNNNGG 8 cut(s) 214, 265, 307, 576, 615, 1158, 1197, 1986
BsmAI GTCTC 2 cut(s) 1847, 2019
BsmFI GGGAC 2 cut(s) 739, 1321
BsoBI CYCGRG 1 cut(s) 1910
Bsp1286I GDGCHC 2 cut(s) 712, 1294
Bsp1407I TGTACA 1 cut(s) 2243
Bsp143I GATC 5 cut(s) 127, 217, 282, 1553, 1579
Bsp19I CCATGG 3 cut(s) 412, 984, 2181
BspACI CCGC 8 cut(s) 51, 79, 94, 181, 208, 266, 606, 1188
BspCNI CTCAG 1 cut(s) 238
BspDI ATCGAT 1 cut(s) 2175
BspFNI CGCG 2 cut(s) 96, 183
BspMAI CTGCAG 4 cut(s) 748, 856, 1330, 1438
BspMI ACCTGC 2 cut(s) 545, 1127
BspPI GGATC 1 cut(s) 225
BspTI CTTAAG 2 cut(s) 1622, 2048
BsrBI CCGCTC 1 cut(s) 53
BsrDI GCAATG 2 cut(s) 437, 1800
BsrFI RCCGGY 1 cut(s) 5
BsrGI TGTACA 1 cut(s) 2243
BsrI ACTGG 3 cut(s) 187, 2039, 2073
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 8 cut(s) 40, 112, 412, 609, 984, 1191, 2083, 2181
BssMI GATC 5 cut(s) 127, 217, 282, 1553, 1579
BssNI GRCGYC 2 cut(s) 938, 1520
BssSI CACGAG 1 cut(s) 2202
BssT1I CCWWGG 4 cut(s) 412, 984, 2083, 2181
Bst2BI CACGAG 1 cut(s) 2202
Bst2UI CCWGG 2 cut(s) 610, 1192
Bst6I CTCTTC 4 cut(s) 359, 489, 1071, 1941
BstACI GRCGYC 2 cut(s) 938, 1520
BstAFI CTTAAG 2 cut(s) 1622, 2048
BstAUI TGTACA 1 cut(s) 2243
BstBAI YACGTR 2 cut(s) 666, 1248
BstC8I GCNNGC 2 cut(s) 7, 55
BstDEI CTNAG 4 cut(s) 246, 327, 453, 2075
BstDSI CCRYGG 3 cut(s) 412, 984, 2181
BstF5I GGATG 7 cut(s) 75, 436, 924, 1008, 1506, 2213, 2246
BstFNI CGCG 2 cut(s) 96, 183
BstHHI GCGC 2 cut(s) 185, 487
BstKTI GATC 5 cut(s) 130, 220, 285, 1556, 1582
BstMAI GTCTC 2 cut(s) 1847, 2019
BstMBI GATC 5 cut(s) 127, 217, 282, 1553, 1579
BstMCI CGRYCG 1 cut(s) 181
BstNI CCWGG 2 cut(s) 610, 1192
BstNSI RCATGY 3 cut(s) 237, 881, 1463
BstSCI CCNGG 4 cut(s) 568, 608, 1150, 1190
BstSFI CTRYAG 5 cut(s) 744, 852, 1326, 1434, 1815
BstUI CGCG 2 cut(s) 96, 183
BstV1I GCAGC 9 cut(s) 527, 567, 730, 866, 1109, 1149, 1171, 1312, 1448
BstV2I GAAGAC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 1553
BstYI RGATCY 1 cut(s) 1553
BstZI CGGCCG 1 cut(s) 178
Bsu15I ATCGAT 1 cut(s) 2175
BsuTUI ATCGAT 1 cut(s) 2175
BtgI CCRYGG 3 cut(s) 412, 984, 2181
BtgZI GCGATG 1 cut(s) 24
BtsCI GGATG 7 cut(s) 75, 436, 924, 1008, 1506, 2213, 2246
BtsI GCAGTG 1 cut(s) 2127
BtsIMutI CAGTG 1 cut(s) 2127
BveI ACCTGC 2 cut(s) 545, 1127
Cac8I GCNNGC 2 cut(s) 7, 55
CfoI GCGC 2 cut(s) 185, 487
Cfr10I RCCGGY 1 cut(s) 5
ClaI ATCGAT 1 cut(s) 2175
CseI GACGC 6 cut(s) 13, 85, 705, 946, 1287, 1528
Csp6I GTAC 4 cut(s) 190, 1039, 2152, 2244
CviQI GTAC 4 cut(s) 190, 1039, 2152, 2244
DdeI CTNAG 4 cut(s) 246, 327, 453, 2075
DpnI GATC 5 cut(s) 129, 219, 284, 1555, 1581
DpnII GATC 5 cut(s) 127, 217, 282, 1553, 1579
DraI TTTAAA 2 cut(s) 1561, 2010
DrdI GACNNNNNNGTC 1 cut(s) 1636
DseDI GACNNNNNNGTC 1 cut(s) 1636
EaeI YGGCCR 4 cut(s) 3, 178, 474, 1056
EagI CGGCCG 1 cut(s) 178
Eam1104I CTCTTC 4 cut(s) 359, 489, 1071, 1941
EarI CTCTTC 4 cut(s) 359, 489, 1071, 1941
EciI GGCGGA 2 cut(s) 68, 197
Ecl136II GAGCTC 2 cut(s) 710, 1292
EclXI CGGCCG 1 cut(s) 178
Eco130I CCWWGG 4 cut(s) 412, 984, 2083, 2181
Eco24I GRGCYC 2 cut(s) 712, 1294
Eco47I GGWCC 7 cut(s) 409, 566, 908, 981, 1148, 1490, 2185
Eco52I CGGCCG 1 cut(s) 178
Eco53kI GAGCTC 2 cut(s) 710, 1292
Eco57I CTGAAG 5 cut(s) 741, 1323, 1595, 1904, 2084
Eco88I CYCGRG 1 cut(s) 1910
EcoICRI GAGCTC 2 cut(s) 710, 1292
EcoRII CCWGG 2 cut(s) 608, 1190
EcoT14I CCWWGG 4 cut(s) 412, 984, 2083, 2181
EcoT22I ATGCAT 1 cut(s) 1732
EcoT38I GRGCYC 2 cut(s) 712, 1294
ErhI CCWWGG 4 cut(s) 412, 984, 2083, 2181
FaqI GGGAC 2 cut(s) 739, 1321
FauNDI CATATG 2 cut(s) 913, 1495
FokI GGATG 7 cut(s) 62, 443, 931, 1015, 1513, 2220, 2233
FriOI GRGCYC 2 cut(s) 712, 1294
GlaI GCGC 2 cut(s) 184, 486
GsaI CCCAGC 1 cut(s) 1971
HapII CCGG 5 cut(s) 6, 33, 177, 570, 1152
HgaI GACGC 6 cut(s) 13, 85, 705, 946, 1287, 1528
HhaI GCGC 2 cut(s) 185, 487
Hin1I GRCGYC 2 cut(s) 938, 1520
Hin6I GCGC 2 cut(s) 183, 485
HinP1I GCGC 2 cut(s) 183, 485
HincII GTYRAC 2 cut(s) 936, 1518
HindII GTYRAC 2 cut(s) 936, 1518
HindIII AAGCTT 5 cut(s) 899, 1481, 1692, 1916, 2193
HinfI GANTC 6 cut(s) 102, 143, 153, 434, 1637, 1840
HpaII CCGG 5 cut(s) 6, 33, 177, 570, 1152
HphI GGTGA 3 cut(s) 22, 584, 1166
Hpy166II GTNNAC 6 cut(s) 891, 936, 1473, 1518, 1851, 2152
Hpy188III TCNNGA 7 cut(s) 131, 812, 945, 1394, 1527, 1613, 1912
Hpy8I GTNNAC 6 cut(s) 891, 936, 1473, 1518, 1851, 2152
Hpy99I CGWCG 3 cut(s) 29, 128, 283
HpyCH4IV ACGT 5 cut(s) 87, 665, 678, 1247, 1260
HpyF3I CTNAG 4 cut(s) 246, 327, 453, 2075
HpySE526I ACGT 5 cut(s) 87, 665, 678, 1247, 1260
Hsp92I GRCGYC 2 cut(s) 938, 1520
HspAI GCGC 2 cut(s) 183, 485
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 5 cut(s) 127, 217, 282, 1553, 1579
LmnI GCTCC 4 cut(s) 274, 303, 2087, 2235
Lsp1109I GCAGC 9 cut(s) 527, 567, 730, 866, 1109, 1149, 1171, 1312, 1448
LweI GCATC 8 cut(s) 154, 331, 855, 1437, 1717, 1814, 2167, 2198
MaeII ACGT 5 cut(s) 87, 665, 678, 1247, 1260
MaeIII GTNAC 7 cut(s) 28, 579, 801, 1161, 1383, 1589, 1953
MalI GATC 5 cut(s) 129, 219, 284, 1555, 1581
MbiI CCGCTC 1 cut(s) 53
MboI GATC 5 cut(s) 127, 217, 282, 1553, 1579
MboII GAAGA 8 cut(s) 82, 218, 376, 506, 1088, 1557, 1958, 2177
MfeI CAATTG 2 cut(s) 870, 1452
MflI RGATCY 1 cut(s) 1553
MhlI GDGCHC 2 cut(s) 712, 1294
MlyI GAGTC 5 cut(s) 152, 162, 443, 1646, 1849
MmeI TCCRAC 1 cut(s) 171
Mph1103I ATGCAT 1 cut(s) 1732
MroNI GCCGGC 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 696, 1278, 1735
MspA1I CMGCKG 2 cut(s) 857, 1439
MspCI CTTAAG 2 cut(s) 1622, 2048
MspI CCGG 5 cut(s) 6, 33, 177, 570, 1152
MspR9I CCNGG 4 cut(s) 570, 610, 1152, 1192
MunI CAATTG 2 cut(s) 870, 1452
MvaI CCWGG 2 cut(s) 610, 1192
MvnI CGCG 2 cut(s) 96, 183
NaeI GCCGGC 1 cut(s) 7
NciI CCSGG 2 cut(s) 570, 1152
NcoI CCATGG 3 cut(s) 412, 984, 2181
NdeI CATATG 2 cut(s) 913, 1495
NdeII GATC 5 cut(s) 127, 217, 282, 1553, 1579
NgoMIV GCCGGC 1 cut(s) 5
NmeAIII GCCGAG 2 cut(s) 65, 93
NmuCI GTSAC 4 cut(s) 28, 801, 1383, 1953
NsiI ATGCAT 1 cut(s) 1732
NspI RCATGY 3 cut(s) 237, 881, 1463
PaeR7I CTCGAG 1 cut(s) 1910
PciI ACATGT 2 cut(s) 877, 1459
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 120, 138
PdiI GCCGGC 1 cut(s) 7
PfeI GAWTC 1 cut(s) 102
PleI GAGTC 5 cut(s) 151, 161, 442, 1645, 1848
PpsI GAGTC 5 cut(s) 151, 161, 442, 1645, 1848
Ppu21I YACGTR 2 cut(s) 666, 1248
PscI ACATGT 2 cut(s) 877, 1459
PsiI TTATAA 2 cut(s) 764, 1346
Psp124BI GAGCTC 2 cut(s) 712, 1294
Psp6I CCWGG 2 cut(s) 608, 1190
PspFI CCCAGC 1 cut(s) 1967
PspGI CCWGG 2 cut(s) 608, 1190
PstI CTGCAG 4 cut(s) 748, 856, 1330, 1438
PsuI RGATCY 1 cut(s) 1553
PvuII CAGCTG 2 cut(s) 857, 1439
RsaI GTAC 4 cut(s) 191, 1040, 2153, 2245
RsaNI GTAC 4 cut(s) 190, 1039, 2152, 2244
RseI CAYNNNNRTG 3 cut(s) 696, 1278, 1735
SacI GAGCTC 2 cut(s) 712, 1294
Sau3AI GATC 5 cut(s) 127, 217, 282, 1553, 1579
SchI GAGTC 5 cut(s) 152, 162, 443, 1646, 1849
ScrFI CCNGG 4 cut(s) 570, 610, 1152, 1192
SduI GDGCHC 2 cut(s) 712, 1294
SfaNI GCATC 8 cut(s) 154, 331, 855, 1437, 1717, 1814, 2167, 2198
SfcI CTRYAG 5 cut(s) 744, 852, 1326, 1434, 1815
Sfr274I CTCGAG 1 cut(s) 1910
SinI GGWCC 7 cut(s) 409, 566, 908, 981, 1148, 1490, 2185
SlaI CTCGAG 1 cut(s) 1910
SmiMI CAYNNNNRTG 3 cut(s) 696, 1278, 1735
SmlI CTYRAG 5 cut(s) 1622, 1651, 1809, 1910, 2048
SmoI CTYRAG 5 cut(s) 1622, 1651, 1809, 1910, 2048
SsiI CCGC 8 cut(s) 51, 79, 94, 181, 208, 266, 606, 1188
SstI GAGCTC 2 cut(s) 712, 1294
StyD4I CCNGG 4 cut(s) 568, 608, 1150, 1190
StyI CCWWGG 4 cut(s) 412, 984, 2083, 2181
TaiI ACGT 5 cut(s) 90, 668, 681, 1250, 1263
TaqI TCGA 9 cut(s) 126, 132, 201, 281, 1582, 1635, 1666, 1911, 2175
TaqII GACCGA 1 cut(s) 298
TatI WGTACW 3 cut(s) 189, 1038, 2243
TauI GCSGC 5 cut(s) 53, 183, 269, 608, 1190
TfiI GAWTC 1 cut(s) 102
TscAI CASTG 1 cut(s) 2134
TseFI GTSAC 4 cut(s) 28, 801, 1383, 1953
TseI GCWGC 9 cut(s) 540, 555, 743, 854, 1122, 1137, 1184, 1325, 1436
Tsp45I GTSAC 4 cut(s) 28, 801, 1383, 1953
TspDTI ATGAA 8 cut(s) 619, 843, 935, 1201, 1425, 1517, 1789, 1858
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 2134
Vha464I CTTAAG 2 cut(s) 1622, 2048
VpaK11BI GGWCC 7 cut(s) 409, 566, 908, 981, 1148, 1490, 2185
XapI RAATTY 1 cut(s) 2237
XbaI TCTAGA 2 cut(s) 811, 1393
XceI RCATGY 3 cut(s) 237, 881, 1463
XhoI CTCGAG 1 cut(s) 1910
Zsp2I ATGCAT 1 cut(s) 1732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.