Rmu_sc0003233.1_g000014

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003233.1
OG0002969
Physical Location & Seq
Forward (+)
45393 .. 47337
1945 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1125 bp
atgtgtaccccgccacaatggactttggcggaactccgcgtcaactgtttctttggatttcttcgcctacatgtcatgtcagtgtgttgcgagtccagactctcagagagaagaagagggaaaacgactgaaagcgagaagaaagcggtttcggccttgggttcctggtttatttatttatttcttttcttttctcctttctgctccactctcgcctcttccccctctaacactgtccccaattcgctccactctaaaaccctaattcttcagccccaacttgctgccactcttgcctcccatccctccaaaatttcgctccaccgtttgagatcggccttaccgcacttccagaataattggctgtgcggtcctcgtttgaagttgcttcctttgagtctgtgttgttccttgtattcaacaatggctggtgaagtagtgactgtaaatggaaatggcaatggaaacacccagctgaatccacagaggacttaccaagttgttgtggctgcaactcgagaaatgggtattggaaaggatgggaaactaccttggaaacttccttctgatctcaagttctttaaggatgtcactgtaaccacatcagatcccggaaaaaagaatgcaattataatgggcagaaaaacatgggagagcattccccctgagcatcggcctctacctggacgccttaatgttgttctgactcgttctgggagttttgatatagctactgcagagaatgtcgtcatatgtgggagcatggcctctgctctggagctgttagctgcttctccttactgtctgtcaattgacaaagtgtttctcataggaggtggccagatattgagggaagctctcaatgggcctggctgtgatgcaattcacattacagaaatagagaccaacattgaatgtgatactttcatccctgcaattgattccactgtatttcagccctggtattcatcttttcccaaggtggagaatagcattcgttattccctcacgacttatgtgcgtgtgaggagttctgaagttggatcccttttgcaagtggtcgatcataaattctctacaaactctaagtttggagatctcaacaagctccaagcttggagataa

Protein Analysis

374

Amino Acids

41.35

Weight (kDa)

9.13

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHFR_1 PF00186 166 - 341 5.1e-37 Dihydrofolate reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 632
AccII CGCG 1 cut(s) 39
AciI CCGC 6 cut(s) 11, 29, 37, 146, 344, 369
AclWI GGATC 3 cut(s) 602, 1038, 1051
AcoI YGGCCR 1 cut(s) 838
AcsI RAATTY 2 cut(s) 312, 1070
AcuI CTGAAG 2 cut(s) 254, 1056
AcyI GRCGYC 1 cut(s) 688
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 683
AflIII ACRYGT 1 cut(s) 70
AgsI TTSAA 3 cut(s) 382, 420, 914
AjnI CCWGG 4 cut(s) 164, 682, 868, 959
AjuI GAANNNNNNNTTGG 2 cut(s) 513, 545
AluBI AGCT 7 cut(s) 475, 731, 781, 788, 857, 1108, 1115
AluI AGCT 7 cut(s) 475, 731, 781, 788, 857, 1108, 1115
Alw26I GTCTC 1 cut(s) 896
AlwI GGATC 3 cut(s) 602, 1038, 1051
Ama87I CYCGRG 1 cut(s) 516
AoxI GGCC 6 cut(s) 153, 336, 674, 765, 838, 866
ApeKI GCWGC 3 cut(s) 284, 509, 788
ApoI RAATTY 2 cut(s) 312, 1070
AspS9I GGNCC 2 cut(s) 371, 866
AsuC2I CCSGG 1 cut(s) 612
AsuHPI GGTGA 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 516
AvaII GGWCC 1 cut(s) 371
BalI TGGCCA 1 cut(s) 840
BamHI GGATCC 1 cut(s) 1043
BbvI GCAGC 3 cut(s) 271, 496, 775
BccI CCATC 2 cut(s) 309, 533
BcgI CGANNNNNNTGC 2 cut(s) 1000, 1034
BciT130I CCWGG 4 cut(s) 166, 684, 870, 961
BcnI CCSGG 1 cut(s) 612
BcoDI GTCTC 1 cut(s) 896
BfmI CTRYAG 1 cut(s) 735
BglII AGATCT 1 cut(s) 1096
BisI GCNGC 3 cut(s) 285, 510, 789
BlsI GCNGC 3 cut(s) 286, 511, 790
Bme1390I CCNGG 5 cut(s) 166, 612, 684, 870, 961
Bme18I GGWCC 1 cut(s) 371
BmeT110I CYCGRG 1 cut(s) 516
BmgT120I GGNCC 2 cut(s) 371, 866
BmiI GGNNCC 2 cut(s) 163, 1045
BmrFI CCNGG 5 cut(s) 166, 612, 684, 870, 961
BmsI GCATC 2 cut(s) 679, 868
BplI GAGNNNNNCTC 2 cut(s) 841, 873
BpmI CTGGAG 1 cut(s) 797
Bpu10I CCTNAGC 1 cut(s) 666
BpuEI CTTGAG 1 cut(s) 557
BpuMI CCSGG 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 688
BsaI GGTCTC 1 cut(s) 896
BsaJI CCNNGG 4 cut(s) 156, 551, 959, 978
Bsc4I CCNNNNNNNGG 1 cut(s) 683
Bse3DI GCAATG 1 cut(s) 466
BseBI CCWGG 4 cut(s) 166, 684, 870, 961
BseDI CCNNGG 4 cut(s) 156, 551, 959, 978
BseGI GGATG 4 cut(s) 301, 544, 592, 927
BseLI CCNNNNNNNGG 1 cut(s) 683
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 2 cut(s) 117, 657
BseRI GAGGAG 1 cut(s) 1042
BseXI GCAGC 3 cut(s) 271, 496, 775
BseYI CCCAGC 1 cut(s) 471
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 6 cut(s) 155, 338, 676, 767, 840, 868
BsiHKCI CYCGRG 1 cut(s) 516
BsiSI CCGG 1 cut(s) 612
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 683
BsmAI GTCTC 1 cut(s) 896
BsmFI GGGAC 1 cut(s) 221
BsmI GAATGC 3 cut(s) 628, 657, 993
BsnI GGCC 6 cut(s) 155, 338, 676, 767, 840, 868
Bso31I GGTCTC 1 cut(s) 896
BsoBI CYCGRG 1 cut(s) 516
Bsp143I GATC 6 cut(s) 332, 568, 607, 1043, 1063, 1096
BspACI CCGC 6 cut(s) 11, 29, 37, 146, 344, 369
BspANI GGCC 6 cut(s) 155, 338, 676, 767, 840, 868
BspCNI CTCAG 2 cut(s) 116, 658
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 163, 1045
BspMAI CTGCAG 1 cut(s) 739
BspPI GGATC 3 cut(s) 602, 1038, 1051
BspTNI GGTCTC 1 cut(s) 896
BsrDI GCAATG 1 cut(s) 466
BssECI CCNNGG 4 cut(s) 156, 551, 959, 978
BssMI GATC 6 cut(s) 332, 568, 607, 1043, 1063, 1096
BssNI GRCGYC 1 cut(s) 688
BssT1I CCWWGG 3 cut(s) 156, 551, 978
Bst2UI CCWGG 4 cut(s) 166, 684, 870, 961
Bst4CI ACNGT 7 cut(s) 47, 235, 326, 445, 595, 803, 949
Bst6I CTCTTC 2 cut(s) 109, 223
BstACI GRCGYC 1 cut(s) 688
BstDEI CTNAG 3 cut(s) 103, 666, 1086
BstF5I GGATG 4 cut(s) 301, 544, 592, 927
BstFNI CGCG 1 cut(s) 39
BstKTI GATC 6 cut(s) 335, 571, 610, 1046, 1066, 1099
BstMAI GTCTC 1 cut(s) 896
BstMBI GATC 6 cut(s) 332, 568, 607, 1043, 1063, 1096
BstMWI GCNNNNNNNGC 2 cut(s) 152, 293
BstNI CCWGG 4 cut(s) 166, 684, 870, 961
BstNSI RCATGY 1 cut(s) 74
BstSCI CCNGG 5 cut(s) 164, 610, 682, 868, 959
BstSFI CTRYAG 1 cut(s) 735
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 271, 496, 775
BstX2I RGATCY 3 cut(s) 607, 1043, 1096
BstYI RGATCY 3 cut(s) 607, 1043, 1096
BsuRI GGCC 6 cut(s) 155, 338, 676, 767, 840, 868
BtsCI GGATG 4 cut(s) 301, 544, 592, 927
BtsIMutI CAGTG 4 cut(s) 87, 231, 591, 945
Cfr13I GGNCC 2 cut(s) 371, 866
CseI GACGC 2 cut(s) 28, 696
Csp6I GTAC 1 cut(s) 6
CviAII CATG 4 cut(s) 71, 76, 648, 763
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 3 cut(s) 103, 666, 1086
DpnI GATC 6 cut(s) 334, 570, 609, 1045, 1065, 1098
DpnII GATC 6 cut(s) 332, 568, 607, 1043, 1063, 1096
EaeI YGGCCR 1 cut(s) 838
Eam1104I CTCTTC 2 cut(s) 109, 223
EarI CTCTTC 2 cut(s) 109, 223
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 3 cut(s) 156, 551, 978
Eco31I GGTCTC 1 cut(s) 896
Eco47I GGWCC 1 cut(s) 371
Eco57I CTGAAG 2 cut(s) 254, 1056
Eco88I CYCGRG 1 cut(s) 516
EcoRII CCWGG 4 cut(s) 164, 682, 868, 959
EcoT14I CCWWGG 3 cut(s) 156, 551, 978
ErhI CCWWGG 3 cut(s) 156, 551, 978
FaeI CATG 4 cut(s) 74, 79, 651, 766
FaqI GGGAC 1 cut(s) 221
FatI CATG 4 cut(s) 70, 75, 647, 762
FauI CCCGC 1 cut(s) 18
FauNDI CATATG 1 cut(s) 752
Fnu4HI GCNGC 3 cut(s) 285, 510, 789
FokI GGATG 4 cut(s) 288, 551, 599, 914
Fsp4HI GCNGC 3 cut(s) 285, 510, 789
GluI GCNGC 3 cut(s) 285, 510, 789
GsaI CCCAGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 797
HaeIII GGCC 6 cut(s) 155, 338, 676, 767, 840, 868
HapII CCGG 1 cut(s) 612
HgaI GACGC 2 cut(s) 28, 696
Hin1I GRCGYC 1 cut(s) 688
Hin1II CATG 4 cut(s) 74, 79, 651, 766
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 1113
HinfI GANTC 6 cut(s) 92, 99, 397, 478, 706, 941
HpaII CCGG 1 cut(s) 612
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 2 cut(s) 6, 43
Hpy188I TCNGA 5 cut(s) 106, 568, 607, 705, 1036
Hpy188III TCNNGA 5 cut(s) 96, 352, 518, 776, 1009
Hpy8I GTNNAC 2 cut(s) 6, 43
HpyAV CCTTC 1 cut(s) 573
HpyCH4III ACNGT 7 cut(s) 47, 235, 326, 445, 595, 803, 949
HpyCH4V TGCA 6 cut(s) 512, 626, 737, 881, 935, 1054
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 293
HpyF3I CTNAG 3 cut(s) 103, 666, 1086
Hsp92I GRCGYC 1 cut(s) 688
Hsp92II CATG 4 cut(s) 74, 79, 651, 766
Kzo9I GATC 6 cut(s) 332, 568, 607, 1043, 1063, 1096
LmnI GCTCC 6 cut(s) 209, 252, 324, 759, 778, 1113
Lsp1109I GCAGC 3 cut(s) 271, 496, 775
LweI GCATC 2 cut(s) 679, 868
MaeIII GTNAC 3 cut(s) 439, 589, 595
MalI GATC 6 cut(s) 334, 570, 609, 1045, 1065, 1098
MboI GATC 6 cut(s) 332, 568, 607, 1043, 1063, 1096
MboII GAAGA 6 cut(s) 53, 123, 126, 151, 210, 260
MfeI CAATTG 2 cut(s) 810, 936
MflI RGATCY 3 cut(s) 607, 1043, 1096
MlsI TGGCCA 1 cut(s) 840
MluCI AATT 9 cut(s) 241, 264, 312, 358, 627, 810, 882, 936, 1070
MluNI TGGCCA 1 cut(s) 840
MlyI GAGTC 4 cut(s) 93, 101, 406, 700
MmeI TCCRAC 1 cut(s) 1021
Mox20I TGGCCA 1 cut(s) 840
MscI TGGCCA 1 cut(s) 840
MseI TTAA 2 cut(s) 582, 693
MslI CAYNNNNRTG 1 cut(s) 80
Msp20I TGGCCA 1 cut(s) 840
MspA1I CMGCKG 1 cut(s) 475
MspI CCGG 1 cut(s) 612
MspR9I CCNGG 5 cut(s) 166, 612, 684, 870, 961
MunI CAATTG 2 cut(s) 810, 936
Mva1269I GAATGC 3 cut(s) 628, 657, 993
MvaI CCWGG 4 cut(s) 166, 684, 870, 961
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 2 cut(s) 152, 293
NciI CCSGG 1 cut(s) 612
NdeI CATATG 1 cut(s) 752
NdeII GATC 6 cut(s) 332, 568, 607, 1043, 1063, 1096
NlaIII CATG 4 cut(s) 74, 79, 651, 766
NlaIV GGNNCC 2 cut(s) 163, 1045
NmuCI GTSAC 2 cut(s) 439, 589
NspI RCATGY 1 cut(s) 74
PaeR7I CTCGAG 1 cut(s) 516
PciI ACATGT 1 cut(s) 70
PctI GAATGC 3 cut(s) 628, 657, 993
PfeI GAWTC 2 cut(s) 478, 941
PfoI TCCNGGA 1 cut(s) 610
PkrI GCNGC 3 cut(s) 286, 511, 790
PleI GAGTC 4 cut(s) 93, 100, 405, 700
PpsI GAGTC 4 cut(s) 93, 100, 405, 700
PscI ACATGT 1 cut(s) 70
PsiI TTATAA 1 cut(s) 632
Psp6I CCWGG 4 cut(s) 164, 682, 868, 959
PspFI CCCAGC 1 cut(s) 471
PspGI CCWGG 4 cut(s) 164, 682, 868, 959
PspN4I GGNNCC 2 cut(s) 163, 1045
PspPI GGNCC 2 cut(s) 371, 866
PstI CTGCAG 1 cut(s) 739
PsuI RGATCY 3 cut(s) 607, 1043, 1096
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 80
SaqAI TTAA 2 cut(s) 582, 693
SatI GCNGC 3 cut(s) 285, 510, 789
Sau3AI GATC 6 cut(s) 332, 568, 607, 1043, 1063, 1096
Sau96I GGNCC 2 cut(s) 371, 866
SchI GAGTC 4 cut(s) 93, 101, 406, 700
ScrFI CCNGG 5 cut(s) 166, 612, 684, 870, 961
SfaNI GCATC 2 cut(s) 679, 868
SfcI CTRYAG 1 cut(s) 735
Sfr274I CTCGAG 1 cut(s) 516
SinI GGWCC 1 cut(s) 371
SlaI CTCGAG 1 cut(s) 516
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 2 cut(s) 516, 572
SmoI CTYRAG 2 cut(s) 516, 572
Sse9I AATT 9 cut(s) 241, 264, 312, 358, 627, 810, 882, 936, 1070
SsiI CCGC 6 cut(s) 11, 29, 37, 146, 344, 369
StyD4I CCNGG 5 cut(s) 164, 610, 682, 868, 959
StyI CCWWGG 3 cut(s) 156, 551, 978
TaaI ACNGT 7 cut(s) 47, 235, 326, 445, 595, 803, 949
TaqI TCGA 2 cut(s) 517, 1062
TasI AATT 9 cut(s) 241, 264, 312, 358, 627, 810, 882, 936, 1070
TfiI GAWTC 2 cut(s) 478, 941
Tru1I TTAA 2 cut(s) 582, 693
Tru9I TTAA 2 cut(s) 582, 693
TscAI CASTG 4 cut(s) 87, 238, 598, 952
TseFI GTSAC 2 cut(s) 439, 589
TseI GCWGC 3 cut(s) 284, 509, 788
Tsp45I GTSAC 2 cut(s) 439, 589
TspDTI ATGAA 2 cut(s) 916, 957
TspRI CASTG 4 cut(s) 87, 238, 598, 952
VpaK11BI GGWCC 1 cut(s) 371
XapI RAATTY 2 cut(s) 312, 1070
XceI RCATGY 1 cut(s) 74
XhoI CTCGAG 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.