Rmu_sc0003624.1_g000020

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003624.1
OG0003064
Physical Location & Seq
Forward (+)
75905 .. 77817
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 933 bp
atggaccctcatatatccatggagggatgcagttattacttggcaatggtactggtccagctagcatatggtggatcaaacgtcctcataaaactttctcttgcccaaggactcaatccaatcgtgtttgtggtttataggcatgttattgccatgctcttgctaggaccatttgcttatgttcttgagaggatggagaaattgcacattaaaagtaccagaagtcaagcaaaggttgttggtacacttctctgcatcggtggttctcttattttcaccttctggaaaggaggacacctattcaagggttttgttgagaggcccctgataactgcatttaacactgagttacggcatggtaagcaaaaccggatcaagggttctgctctaattctgactagtcatatagcatggtgttcatggctaattctccaggctgtggtctccaaagtctaccctgctccgttgtcactaaccaccatcatatgctttttcgcatcattgcaatcttcctttcttgccttgttctttgcaagaaatccagcttcgtggagacttgagtggaacctacagcttttaaccatcatctactctggagttgtgttgtcagcattagtctattacctgcaagcgtggtgcatcagttacaagggaccagtttttgcggctatgtttagccccctacaagtcattatagtggcaatgttctcggcagttgcttttgcagagcgacttcactttggcagcttgatcggagcatttctcattatcgttggcctttactgtgtgctatggggaaagaggcaagatggtcttgttgctgaacccccacagagtggacatggagttcttaacaatgataaagtgctcgcaatttccaagattgatattcaagtgatacatcctgtaactagagaaagtgcatgttcataa

Protein Analysis

310

Amino Acids

34.3

Weight (kDa)

9.41

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 126 - 264 2.4e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 633
AccB7I CCANNNNNTGG 2 cut(s) 439, 836
AccI GTMKAC 1 cut(s) 453
AciI CCGC 1 cut(s) 665
AclWI GGATC 2 cut(s) 82, 380
AdeI CACNNNGTG 1 cut(s) 836
AfaI GTAC 3 cut(s) 51, 217, 244
AfiI CCNNNNNNNGG 4 cut(s) 304, 376, 439, 836
AgsI TTSAA 2 cut(s) 304, 893
AhlI ACTAGT 1 cut(s) 398
AjnI CCWGG 1 cut(s) 432
AloI GAACNNNNNNTCC 4 cut(s) 364, 396, 831, 863
AluBI AGCT 4 cut(s) 61, 545, 574, 747
AluI AGCT 4 cut(s) 61, 545, 574, 747
Alw21I GWGCWC 1 cut(s) 870
Alw26I GTCTC 2 cut(s) 448, 547
AlwI GGATC 2 cut(s) 82, 380
AoxI GGCC 2 cut(s) 320, 775
ApeKI GCWGC 1 cut(s) 744
AspS9I GGNCC 5 cut(s) 4, 55, 167, 321, 653
AsuHPI GGTGA 1 cut(s) 268
AsuNHI GCTAGC 1 cut(s) 61
AvaII GGWCC 4 cut(s) 4, 55, 167, 653
Bbv12I GWGCWC 1 cut(s) 870
BbvI GCAGC 1 cut(s) 756
BccI CCATC 4 cut(s) 187, 488, 590, 803
BceAI ACGGC 1 cut(s) 368
BcgI CGANNNNNNTGC 4 cut(s) 690, 724, 733, 767
BciT130I CCWGG 1 cut(s) 434
BcoDI GTCTC 2 cut(s) 448, 547
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 4 cut(s) 62, 164, 399, 912
BfmI CTRYAG 1 cut(s) 569
BfuAI ACCTGC 1 cut(s) 633
BisI GCNGC 2 cut(s) 666, 745
BlsI GCNGC 2 cut(s) 667, 746
Bme1390I CCNGG 1 cut(s) 434
Bme18I GGWCC 4 cut(s) 4, 55, 167, 653
BmgT120I GGNCC 5 cut(s) 4, 55, 167, 321, 653
BmiI GGNNCC 4 cut(s) 6, 323, 566, 654
BmrFI CCNGG 1 cut(s) 434
BmsI GCATC 4 cut(s) 17, 264, 506, 648
BmtI GCTAGC 1 cut(s) 65
BplI GAGNNNNNCTC 2 cut(s) 747, 779
BpmI CTGGAG 2 cut(s) 416, 615
BpuEI CTTGAG 2 cut(s) 206, 578
BsaI GGTCTC 1 cut(s) 448
BsaJI CCNNGG 2 cut(s) 18, 106
BsaWI WCCGGW 1 cut(s) 369
Bsc4I CCNNNNNNNGG 4 cut(s) 304, 376, 439, 836
Bse1I ACTGG 2 cut(s) 57, 656
Bse3DI GCAATG 3 cut(s) 51, 500, 708
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 2 cut(s) 18, 106
BseGI GGATG 3 cut(s) 32, 198, 901
BseLI CCNNNNNNNGG 4 cut(s) 304, 376, 439, 836
BseMI GCAATG 3 cut(s) 51, 500, 708
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 2 cut(s) 57, 656
BseXI GCAGC 1 cut(s) 756
BshFI GGCC 2 cut(s) 322, 777
BsiHKAI GWGCWC 1 cut(s) 870
BsiSI CCGG 1 cut(s) 370
BslFI GGGAC 1 cut(s) 666
BslI CCNNNNNNNGG 4 cut(s) 304, 376, 439, 836
BsmAI GTCTC 2 cut(s) 448, 547
BsmFI GGGAC 1 cut(s) 666
BsnI GGCC 2 cut(s) 322, 777
Bso31I GGTCTC 1 cut(s) 448
Bsp1286I GDGCHC 1 cut(s) 870
Bsp143I GATC 3 cut(s) 74, 372, 750
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 1 cut(s) 665
BspANI GGCC 2 cut(s) 322, 777
BspCNI CTCAG 1 cut(s) 337
BspLI GGNNCC 4 cut(s) 6, 323, 566, 654
BspMI ACCTGC 1 cut(s) 633
BspOI GCTAGC 1 cut(s) 65
BspPI GGATC 2 cut(s) 82, 380
BspTNI GGTCTC 1 cut(s) 448
BsrDI GCAATG 3 cut(s) 51, 500, 708
BsrI ACTGG 2 cut(s) 57, 656
BssECI CCNNGG 2 cut(s) 18, 106
BssMI GATC 3 cut(s) 74, 372, 750
BssT1I CCWWGG 2 cut(s) 18, 106
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 1 cut(s) 785
BstC8I GCNNGC 3 cut(s) 63, 630, 870
BstDEI CTNAG 1 cut(s) 345
BstDSI CCRYGG 1 cut(s) 18
BstENI CCTNNNNNAGG 1 cut(s) 302
BstF5I GGATG 3 cut(s) 32, 198, 901
BstKTI GATC 3 cut(s) 77, 375, 753
BstMAI GTCTC 2 cut(s) 448, 547
BstMBI GATC 3 cut(s) 74, 372, 750
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 2 cut(s) 146, 927
BstSCI CCNGG 1 cut(s) 432
BstSFI CTRYAG 1 cut(s) 569
BstV1I GCAGC 1 cut(s) 756
BstXI CCANNNNNNTGG 1 cut(s) 549
BsuRI GGCC 2 cut(s) 322, 777
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 3 cut(s) 32, 198, 901
BtsIMutI CAGTG 1 cut(s) 342
BveI ACCTGC 1 cut(s) 633
Cac8I GCNNGC 3 cut(s) 63, 630, 870
Cfr13I GGNCC 5 cut(s) 4, 55, 167, 321, 653
Csp6I GTAC 3 cut(s) 50, 216, 243
CviAII CATG 8 cut(s) 19, 143, 154, 356, 411, 420, 842, 924
CviQI GTAC 3 cut(s) 50, 216, 243
DdeI CTNAG 1 cut(s) 345
DpnI GATC 3 cut(s) 76, 374, 752
DpnII GATC 3 cut(s) 74, 372, 750
DraIII CACNNNGTG 1 cut(s) 836
Eco130I CCWWGG 2 cut(s) 18, 106
Eco31I GGTCTC 1 cut(s) 448
Eco47I GGWCC 4 cut(s) 4, 55, 167, 653
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 432
EcoT14I CCWWGG 2 cut(s) 18, 106
ErhI CCWWGG 2 cut(s) 18, 106
FaeI CATG 8 cut(s) 22, 146, 157, 359, 414, 423, 845, 927
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FaqI GGGAC 1 cut(s) 666
FatI CATG 8 cut(s) 18, 142, 153, 355, 410, 419, 841, 923
FauNDI CATATG 2 cut(s) 67, 485
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 2 cut(s) 666, 745
FokI GGATG 3 cut(s) 39, 205, 888
Fsp4HI GCNGC 2 cut(s) 666, 745
FspBI CTAG 4 cut(s) 62, 164, 399, 912
GluI GCNGC 2 cut(s) 666, 745
GsuI CTGGAG 2 cut(s) 416, 615
HaeIII GGCC 2 cut(s) 322, 777
HapII CCGG 1 cut(s) 370
Hin1II CATG 8 cut(s) 22, 146, 157, 359, 414, 423, 845, 927
HinfI GANTC 1 cut(s) 111
HpaII CCGG 1 cut(s) 370
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 3 cut(s) 245, 454, 839
Hpy188I TCNGA 2 cut(s) 396, 755
Hpy188III TCNNGA 3 cut(s) 185, 283, 594
Hpy8I GTNNAC 3 cut(s) 245, 454, 839
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 1 cut(s) 785
HpyCH4IV ACGT 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 8 cut(s) 22, 146, 157, 359, 414, 423, 845, 927
Kzo9I GATC 3 cut(s) 74, 372, 750
LmnI GCTCC 2 cut(s) 466, 755
Lsp1109I GCAGC 1 cut(s) 756
LweI GCATC 4 cut(s) 17, 264, 506, 648
MaeI CTAG 4 cut(s) 62, 164, 399, 912
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 4 cut(s) 348, 468, 644, 907
MalI GATC 3 cut(s) 76, 374, 752
MboI GATC 3 cut(s) 74, 372, 750
MboII GAAGA 1 cut(s) 501
MhlI GDGCHC 1 cut(s) 870
MluCI AATT 4 cut(s) 200, 390, 426, 873
MlyI GAGTC 1 cut(s) 105
MnlI CCTC 7 cut(s) 16, 18, 95, 183, 284, 312, 795
MseI TTAA 4 cut(s) 210, 339, 578, 852
MslI CAYNNNNRTG 1 cut(s) 695
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 1 cut(s) 434
MvaI CCWGG 1 cut(s) 434
MwoI GCNNNNNNNGC 1 cut(s) 361
NcoI CCATGG 1 cut(s) 18
NdeI CATATG 2 cut(s) 67, 485
NdeII GATC 3 cut(s) 74, 372, 750
NheI GCTAGC 1 cut(s) 61
NlaIII CATG 8 cut(s) 22, 146, 157, 359, 414, 423, 845, 927
NlaIV GGNNCC 4 cut(s) 6, 323, 566, 654
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 1 cut(s) 468
NspI RCATGY 2 cut(s) 146, 927
PflMI CCANNNNNTGG 2 cut(s) 439, 836
PkrI GCNGC 2 cut(s) 667, 746
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 432
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 4 cut(s) 6, 323, 566, 654
PspPI GGNCC 5 cut(s) 4, 55, 167, 321, 653
RsaI GTAC 3 cut(s) 51, 217, 244
RsaNI GTAC 3 cut(s) 50, 216, 243
RseI CAYNNNNRTG 1 cut(s) 695
SaqAI TTAA 4 cut(s) 210, 339, 578, 852
SatI GCNGC 2 cut(s) 666, 745
Sau3AI GATC 3 cut(s) 74, 372, 750
Sau96I GGNCC 5 cut(s) 4, 55, 167, 321, 653
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 1 cut(s) 434
SduI GDGCHC 1 cut(s) 870
SfaNI GCATC 4 cut(s) 17, 264, 506, 648
SfcI CTRYAG 1 cut(s) 569
SinI GGWCC 4 cut(s) 4, 55, 167, 653
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 2 cut(s) 185, 557
SmoI CTYRAG 2 cut(s) 185, 557
SpeI ACTAGT 1 cut(s) 398
Sse9I AATT 4 cut(s) 200, 390, 426, 873
SsiI CCGC 1 cut(s) 665
SspMI CTAG 4 cut(s) 62, 164, 399, 912
StyD4I CCNGG 1 cut(s) 432
StyI CCWWGG 2 cut(s) 18, 106
TaaI ACNGT 1 cut(s) 785
TaiI ACGT 1 cut(s) 84
TasI AATT 4 cut(s) 200, 390, 426, 873
TauI GCSGC 1 cut(s) 668
Tru1I TTAA 4 cut(s) 210, 339, 578, 852
Tru9I TTAA 4 cut(s) 210, 339, 578, 852
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 1 cut(s) 468
TseI GCWGC 1 cut(s) 744
Tsp45I GTSAC 1 cut(s) 468
TspDTI ATGAA 2 cut(s) 408, 918
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 1 cut(s) 349
Van91I CCANNNNNTGG 2 cut(s) 439, 836
VpaK11BI GGWCC 4 cut(s) 4, 55, 167, 653
XagI CCTNNNNNAGG 1 cut(s) 302
XceI RCATGY 2 cut(s) 146, 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 453
XspI CTAG 4 cut(s) 62, 164, 399, 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.