Rmu_sc0004082.1_g000025

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004082.1
OG0009711
Physical Location & Seq
Reverse (-)
108254 .. 113249
4996 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1290 bp
atgagcagctgggttggagcttcttcttctccttctccatggcctctcaggacctcttcctcctcctatccttccaaattcttcaaattccattcatgtcctcctcctccttctccgcctcctctgtttcgtcctctctgcctcgcccaatccaaccacgtcggcagcgacctcaaattcgtgctccacgacgctctcgactcttctggaatcctcccctctcacgccagagatgctagggagggattctgctcgcaaatcggcaaaatgtctgatatagagaggcaaaccagcattagcatcaaccgccgcgttgatttagggaaaactgctctttatattgcagcagaggatgactccctcgtgtcgcattcgtccgttccgcttccagtggatgcttttatcagtagatttgatgatctttctatggactattgttctcactacaactcttcattgaaatcgtcgccggagaggttgttggagagtttggaaaattatttgtacatacacaagggattccgaagaaccaacgcaaggcatctcttagaaacacgagctctatatctccattcggttttgacacatcgttcaggatcaactgcaatgctatcactaatatactcagaaattctgaaaatgcttcgcttatggggacttttggattttgatgtggagatattctttcctcacgatcctcatagtcttcccagaggctatcccaaaaagaaaagcaaggaatctgatcatgcacacataatgacatcacaagcccttttggttgagattctaagcaatttgaaggatgctttctggccttttcagcatgatcaatcagggaatttattcttaagggctgcacgggctgctaacttcattgatgcatcaaatgttgatgaagacagtggctttcagcttgcatctgctaaggctgctcaacataggctagatcgtggtgtttggaccagtgttaattttggggatatgaggcgtgcattatctgcttgcgaacgtcttattctcctcgaagctgattcaaaggaattgagagattatagcattcttctctaccattgtgggttttatgagcaatcacttgaatttttaaagttgtatcaggacacaaagagtcgcccacaacttagacgatcatctaattcatcaaccaatctagaggaagatgctgtagagaagttgatgatacgccttaaactaattttgatggaggatggttggagcaggccttcatatgtaagaaactttcttggtaacagctctgatccatggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transglut_core2

Protein Analysis

429

Amino Acids

48.67

Weight (kDa)

7.0

Isoelectric Point (pI)

59.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transglut_core2 PF13369 140 - 216 5e-07 Transglutaminase-like superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 312
AciI CCGC 4 cut(s) 116, 307, 310, 383
AclWI GGATC 3 cut(s) 604, 689, 1274
AcsI RAATTY 6 cut(s) 77, 86, 176, 630, 841, 1100
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 1 cut(s) 537
AflII CTTAAG 1 cut(s) 850
AgsI TTSAA 5 cut(s) 85, 460, 802, 1038, 1100
AjiI CACGTC 1 cut(s) 160
AluBI AGCT 6 cut(s) 9, 20, 560, 916, 1031, 1275
AluI AGCT 6 cut(s) 9, 20, 560, 916, 1031, 1275
Alw21I GWGCWC 2 cut(s) 186, 562
AlwI GGATC 3 cut(s) 604, 689, 1274
AoxI GGCC 3 cut(s) 41, 815, 1241
ApeKI GCWGC 6 cut(s) 6, 165, 344, 857, 866, 932
ApoI RAATTY 6 cut(s) 77, 86, 176, 630, 841, 1100
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
Asp700I GAANNNNTTC 1 cut(s) 845
AspS9I GGNCC 2 cut(s) 51, 963
AvaII GGWCC 2 cut(s) 51, 963
BanII GRGCYC 1 cut(s) 562
BauI CACGAG 2 cut(s) 362, 555
BbsI GAAGAC 2 cut(s) 698, 906
Bbv12I GWGCWC 2 cut(s) 186, 562
BbvI GCAGC 6 cut(s) 18, 177, 356, 844, 853, 919
BccI CCATC 2 cut(s) 1216, 1223
BclI TGATCA 2 cut(s) 745, 829
BfaI CTAG 3 cut(s) 237, 947, 1172
BfmI CTRYAG 1 cut(s) 1185
BfrI CTTAAG 1 cut(s) 850
BisI GCNGC 7 cut(s) 7, 166, 310, 345, 858, 867, 933
BlsI GCNGC 7 cut(s) 8, 167, 311, 346, 859, 868, 934
Bme18I GGWCC 2 cut(s) 51, 963
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 51, 963
BmsI GCATC 9 cut(s) 223, 309, 385, 550, 796, 871, 893, 929, 1171
BpiI GAAGAC 2 cut(s) 698, 906
BplI GAGNNNNNCTC 2 cut(s) 341, 373
Bpu10I CCTNAGC 1 cut(s) 927
BsaJI CCNNGG 2 cut(s) 38, 1283
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 1 cut(s) 537
Bse1I ACTGG 2 cut(s) 389, 966
Bse3DI GCAATG 1 cut(s) 612
BseDI CCNNGG 2 cut(s) 38, 1283
BseGI GGATG 4 cut(s) 358, 400, 811, 1234
BseLI CCNNNNNNNGG 1 cut(s) 537
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 2 cut(s) 61, 639
BseNI ACTGG 2 cut(s) 389, 966
BseRI GAGGAG 5 cut(s) 52, 93, 96, 111, 1013
BseXI GCAGC 6 cut(s) 18, 177, 356, 844, 853, 919
BseYI CCCAGC 1 cut(s) 9
BsgI GTGCAG 1 cut(s) 843
Bsh1236I CGCG 1 cut(s) 312
BshFI GGCC 3 cut(s) 43, 817, 1243
BsiHKAI GWGCWC 2 cut(s) 186, 562
BsiSI CCGG 1 cut(s) 470
BslFI GGGAC 1 cut(s) 669
BslI CCNNNNNNNGG 1 cut(s) 537
BsmFI GGGAC 1 cut(s) 669
BsmI GAATGC 2 cut(s) 370, 1059
BsnI GGCC 3 cut(s) 43, 817, 1243
Bsp1286I GDGCHC 2 cut(s) 186, 562
Bsp1407I TGTACA 1 cut(s) 504
Bsp143I GATC 8 cut(s) 418, 596, 694, 745, 829, 949, 1148, 1279
Bsp19I CCATGG 2 cut(s) 38, 1283
BspACI CCGC 4 cut(s) 116, 307, 310, 383
BspANI GGCC 3 cut(s) 43, 817, 1243
BspCNI CTCAG 2 cut(s) 60, 638
BspFNI CGCG 1 cut(s) 312
BspPI GGATC 3 cut(s) 604, 689, 1274
BspTI CTTAAG 1 cut(s) 850
BsrDI GCAATG 1 cut(s) 612
BsrGI TGTACA 1 cut(s) 504
BsrI ACTGG 2 cut(s) 389, 966
BssECI CCNNGG 2 cut(s) 38, 1283
BssMI GATC 8 cut(s) 418, 596, 694, 745, 829, 949, 1148, 1279
BssSI CACGAG 2 cut(s) 362, 555
BssT1I CCWWGG 2 cut(s) 38, 1283
Bst2BI CACGAG 2 cut(s) 362, 555
Bst4CI ACNGT 1 cut(s) 905
Bst6I CTCTTC 3 cut(s) 61, 208, 457
BstAFI CTTAAG 1 cut(s) 850
BstAPI GCANNNNNTGC 2 cut(s) 866, 1001
BstAUI TGTACA 1 cut(s) 504
BstC8I GCNNGC 5 cut(s) 254, 918, 993, 1006, 1241
BstDEI CTNAG 6 cut(s) 47, 547, 625, 791, 927, 1142
BstDSI CCRYGG 2 cut(s) 38, 1283
BstF5I GGATG 4 cut(s) 358, 400, 811, 1234
BstFNI CGCG 1 cut(s) 312
BstKTI GATC 8 cut(s) 421, 599, 697, 748, 832, 952, 1151, 1282
BstMBI GATC 8 cut(s) 418, 596, 694, 745, 829, 949, 1148, 1279
BstMWI GCNNNNNNNGC 7 cut(s) 233, 306, 823, 863, 866, 932, 1001
BstSFI CTRYAG 1 cut(s) 1185
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 6 cut(s) 18, 177, 356, 844, 853, 919
BstV2I GAAGAC 2 cut(s) 698, 906
BsuRI GGCC 3 cut(s) 43, 817, 1243
BtgI CCRYGG 2 cut(s) 38, 1283
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 4 cut(s) 358, 400, 811, 1234
BtsIMutI CAGTG 3 cut(s) 396, 910, 973
Cac8I GCNNGC 5 cut(s) 254, 918, 993, 1006, 1241
Cfr13I GGNCC 2 cut(s) 51, 963
CseI GACGC 1 cut(s) 200
Csp6I GTAC 1 cut(s) 505
CviAII CATG 5 cut(s) 39, 96, 749, 827, 1284
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 6 cut(s) 47, 547, 625, 791, 927, 1142
DpnI GATC 8 cut(s) 420, 598, 696, 747, 831, 951, 1150, 1281
DpnII GATC 8 cut(s) 418, 596, 694, 745, 829, 949, 1148, 1279
DraI TTTAAA 1 cut(s) 1107
Eam1104I CTCTTC 3 cut(s) 61, 208, 457
EarI CTCTTC 3 cut(s) 61, 208, 457
EciI GGCGGA 1 cut(s) 105
Ecl136II GAGCTC 1 cut(s) 560
Eco130I CCWWGG 2 cut(s) 38, 1283
Eco147I AGGCCT 1 cut(s) 1243
Eco24I GRGCYC 1 cut(s) 562
Eco47I GGWCC 2 cut(s) 51, 963
Eco53kI GAGCTC 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 51
EcoT14I CCWWGG 2 cut(s) 38, 1283
EcoT22I ATGCAT 1 cut(s) 886
EcoT38I GRGCYC 1 cut(s) 562
ErhI CCWWGG 2 cut(s) 38, 1283
FaeI CATG 5 cut(s) 42, 99, 752, 830, 1287
FaqI GGGAC 1 cut(s) 669
FatI CATG 5 cut(s) 38, 95, 748, 826, 1283
FauNDI CATATG 1 cut(s) 1249
FbaI TGATCA 2 cut(s) 745, 829
Fnu4HI GCNGC 7 cut(s) 7, 166, 310, 345, 858, 867, 933
FokI GGATG 4 cut(s) 365, 407, 818, 1241
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 7 cut(s) 7, 166, 310, 345, 858, 867, 933
FspBI CTAG 3 cut(s) 237, 947, 1172
GluI GCNGC 7 cut(s) 7, 166, 310, 345, 858, 867, 933
GsaI CCCAGC 1 cut(s) 13
HaeIII GGCC 3 cut(s) 43, 817, 1243
HapII CCGG 1 cut(s) 470
HgaI GACGC 1 cut(s) 200
Hin1II CATG 5 cut(s) 42, 99, 752, 830, 1287
HinfI GANTC 9 cut(s) 200, 210, 246, 356, 519, 740, 787, 1034, 1129
HpaII CCGG 1 cut(s) 470
Hpy188I TCNGA 6 cut(s) 274, 524, 628, 636, 745, 1279
Hpy188III TCNNGA 7 cut(s) 49, 197, 207, 594, 692, 1118, 1172
Hpy99I CGWCG 3 cut(s) 164, 194, 469
HpyAV CCTTC 5 cut(s) 42, 81, 120, 796, 1254
HpyCH4III ACNGT 1 cut(s) 905
HpyCH4IV ACGT 2 cut(s) 159, 1012
HpyCH4V TGCA 7 cut(s) 344, 605, 752, 860, 884, 920, 995
HpyF10VI GCNNNNNNNGC 7 cut(s) 233, 306, 823, 863, 866, 932, 1001
HpyF3I CTNAG 6 cut(s) 47, 547, 625, 791, 927, 1142
HpySE526I ACGT 2 cut(s) 159, 1012
Hsp92II CATG 5 cut(s) 42, 99, 752, 830, 1287
Ksp22I TGATCA 2 cut(s) 745, 829
Kzo9I GATC 8 cut(s) 418, 596, 694, 745, 829, 949, 1148, 1279
LmnI GCTCC 3 cut(s) 17, 189, 1236
Lsp1109I GCAGC 6 cut(s) 18, 177, 356, 844, 853, 919
LweI GCATC 9 cut(s) 223, 309, 385, 550, 796, 871, 893, 929, 1171
MaeI CTAG 3 cut(s) 237, 947, 1172
MaeII ACGT 2 cut(s) 159, 1012
MaeIII GTNAC 1 cut(s) 1268
MalI GATC 8 cut(s) 420, 598, 696, 747, 831, 951, 1150, 1281
MboI GATC 8 cut(s) 418, 596, 694, 745, 829, 949, 1148, 1279
MhlI GDGCHC 2 cut(s) 186, 562
MlyI GAGTC 3 cut(s) 194, 350, 1138
MmeI TCCRAC 3 cut(s) 177, 462, 1214
Mph1103I ATGCAT 1 cut(s) 886
MroXI GAANNNNTTC 1 cut(s) 845
MseI TTAA 4 cut(s) 851, 972, 1106, 1209
MspA1I CMGCKG 1 cut(s) 9
MspCI CTTAAG 1 cut(s) 850
MspI CCGG 1 cut(s) 470
Mva1269I GAATGC 2 cut(s) 370, 1059
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 7 cut(s) 233, 306, 823, 863, 866, 932, 1001
NcoI CCATGG 2 cut(s) 38, 1283
NdeI CATATG 1 cut(s) 1249
NdeII GATC 8 cut(s) 418, 596, 694, 745, 829, 949, 1148, 1279
NlaIII CATG 5 cut(s) 42, 99, 752, 830, 1287
NsiI ATGCAT 1 cut(s) 886
PceI AGGCCT 1 cut(s) 1243
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 186, 195
PctI GAATGC 2 cut(s) 370, 1059
PdmI GAANNNNTTC 1 cut(s) 845
PfeI GAWTC 6 cut(s) 210, 246, 519, 740, 787, 1034
PkrI GCNGC 7 cut(s) 8, 167, 311, 346, 859, 868, 934
PleI GAGTC 3 cut(s) 194, 350, 1137
PpsI GAGTC 3 cut(s) 194, 350, 1137
PpuMI RGGWCCY 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 562
Psp5II RGGWCCY 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 9
PspPI GGNCC 2 cut(s) 51, 963
PspPPI RGGWCCY 1 cut(s) 51
PvuII CAGCTG 1 cut(s) 9
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacI GAGCTC 1 cut(s) 562
SaqAI TTAA 4 cut(s) 851, 972, 1106, 1209
SatI GCNGC 7 cut(s) 7, 166, 310, 345, 858, 867, 933
Sau3AI GATC 8 cut(s) 418, 596, 694, 745, 829, 949, 1148, 1279
Sau96I GGNCC 2 cut(s) 51, 963
SchI GAGTC 3 cut(s) 194, 350, 1138
SduI GDGCHC 2 cut(s) 186, 562
SfaNI GCATC 9 cut(s) 223, 309, 385, 550, 796, 871, 893, 929, 1171
SfcI CTRYAG 1 cut(s) 1185
SinI GGWCC 2 cut(s) 51, 963
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
SseBI AGGCCT 1 cut(s) 1243
SsiI CCGC 4 cut(s) 116, 307, 310, 383
SspMI CTAG 3 cut(s) 237, 947, 1172
SstI GAGCTC 1 cut(s) 562
StuI AGGCCT 1 cut(s) 1243
StyI CCWWGG 2 cut(s) 38, 1283
TaaI ACNGT 1 cut(s) 905
TaiI ACGT 2 cut(s) 162, 1015
TaqI TCGA 2 cut(s) 198, 1026
TatI WGTACW 1 cut(s) 504
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 6 cut(s) 210, 246, 519, 740, 787, 1034
Tru1I TTAA 4 cut(s) 851, 972, 1106, 1209
Tru9I TTAA 4 cut(s) 851, 972, 1106, 1209
TscAI CASTG 3 cut(s) 396, 910, 973
TseI GCWGC 6 cut(s) 6, 165, 344, 857, 866, 932
TspDTI ATGAA 6 cut(s) 84, 444, 865, 912, 1149, 1236
TspGWI ACGGA 1 cut(s) 367
TspRI CASTG 3 cut(s) 396, 910, 973
Vha464I CTTAAG 1 cut(s) 850
VpaK11BI GGWCC 2 cut(s) 51, 963
XapI RAATTY 6 cut(s) 77, 86, 176, 630, 841, 1100
XbaI TCTAGA 1 cut(s) 1171
XmnI GAANNNNTTC 1 cut(s) 845
XspI CTAG 3 cut(s) 237, 947, 1172
Zsp2I ATGCAT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.