Rmu_sc0004171.1_g000005

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004171.1
OG0004631
Physical Location & Seq
Reverse (-)
22351 .. 23917
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
atgatggcatcccagtttctggcagcggcggcggagaggatagctccggcggcgaggcgtcaagccgtcatcttaacagacgctgcggcatctagagtacgacggctcctagagcagcgccagcggtcgtatctcaagctcggcgtcaagtcccgaggctgcaacggcttgtcgtacactctcaattacgcagatgataaaggcaagttcgacgagttgatcgaagacaagggtgtgaagatattgatcgatccaaaggctctgatgcatgttattgggaccaagatggattttgtagatgataagctcaggtccgagtttgtctttatcaatcccaactccaaaggacaatgtggttgtggtgaatctttcatgacaacaagcagtagtggagctactaaacagagtgatggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

138

Amino Acids

15.1

Weight (kDa)

9.24

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fe-S_biosyn PF01521 24 - 122 1.2e-18 Iron-sulphur cluster biosynthesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 19
AciI CCGC 6 cut(s) 26, 29, 32, 50, 86, 124
AclWI GGATC 1 cut(s) 245
AcyI GRCGYC 2 cut(s) 58, 144
AfaI GTAC 2 cut(s) 99, 176
AfiI CCNNNNNNNGG 1 cut(s) 19
AluBI AGCT 4 cut(s) 44, 139, 307, 395
AluI AGCT 4 cut(s) 44, 139, 307, 395
AlwI GGATC 1 cut(s) 245
AlwNI CAGNNNCTG 2 cut(s) 19, 83
Ama87I CYCGRG 1 cut(s) 153
ApeKI GCWGC 4 cut(s) 23, 83, 115, 159
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 2 cut(s) 279, 312
AsuHPI GGTGA 1 cut(s) 374
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 279, 312
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 35, 70, 127, 146
BccI CCATC 2 cut(s) 280, 404
BceAI ACGGC 3 cut(s) 50, 119, 181
BfaI CTAG 2 cut(s) 93, 110
BfoI RGCGCY 1 cut(s) 121
BisI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
BlsI GCNGC 8 cut(s) 25, 28, 31, 52, 85, 88, 117, 161
Bme18I GGWCC 2 cut(s) 279, 312
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 2 cut(s) 279, 312
BmiI GGNNCC 2 cut(s) 107, 280
BmrI ACTGGG 1 cut(s) 7
BmsI GCATC 3 cut(s) 17, 98, 255
BmuI ACTGGG 1 cut(s) 7
BpiI GAAGAC 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 28, 60
Bpu10I CCTNAGC 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 249
BsaBI GATNNNNATC 1 cut(s) 245
BsaHI GRCGYC 2 cut(s) 58, 144
BsaJI CCNNGG 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 13
Bse8I GATNNNNATC 1 cut(s) 245
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 1 cut(s) 8
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 1 cut(s) 19
BseMII CTCAG 1 cut(s) 322
BseNI ACTGG 1 cut(s) 13
BseXI GCAGC 4 cut(s) 35, 70, 127, 146
Bsh1285I CGRYCG 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 2 cut(s) 136, 292
BslI CCNNNNNNNGG 1 cut(s) 19
BsmFI GGGAC 2 cut(s) 136, 292
BsoBI CYCGRG 1 cut(s) 153
Bsp143I GATC 3 cut(s) 219, 246, 250
BspACI CCGC 6 cut(s) 26, 29, 32, 50, 86, 124
BspCNI CTCAG 1 cut(s) 321
BspDI ATCGAT 1 cut(s) 249
BspHI TCATGA 1 cut(s) 372
BspLI GGNNCC 2 cut(s) 107, 280
BspPI GGATC 1 cut(s) 245
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 3 cut(s) 219, 246, 250
BssNI GRCGYC 2 cut(s) 58, 144
BstACI GRCGYC 2 cut(s) 58, 144
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 1 cut(s) 308
BstF5I GGATG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 3 cut(s) 222, 249, 253
BstMBI GATC 3 cut(s) 219, 246, 250
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 5 cut(s) 29, 50, 112, 121, 165
BstNSI RCATGY 1 cut(s) 272
BstV1I GCAGC 4 cut(s) 35, 70, 127, 146
BstV2I GAAGAC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 249
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 1 cut(s) 8
Cac8I GCNNGC 1 cut(s) 122
CaiI CAGNNNCTG 2 cut(s) 19, 83
CciI TCATGA 1 cut(s) 372
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 2 cut(s) 279, 312
ClaI ATCGAT 1 cut(s) 249
CseI GACGC 3 cut(s) 47, 89, 133
Csp6I GTAC 2 cut(s) 98, 175
CviAII CATG 2 cut(s) 269, 373
CviJI RGCY 9 cut(s) 44, 65, 106, 139, 159, 168, 260, 307, 395
CviKI_1 RGCY 9 cut(s) 44, 65, 106, 139, 159, 168, 260, 307, 395
CviQI GTAC 2 cut(s) 98, 175
DdeI CTNAG 1 cut(s) 308
DpnI GATC 3 cut(s) 221, 248, 252
DpnII GATC 3 cut(s) 219, 246, 250
EciI GGCGGA 1 cut(s) 47
Eco47I GGWCC 2 cut(s) 279, 312
Eco88I CYCGRG 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 270
FaeI CATG 2 cut(s) 272, 376
FaiI YATR 2 cut(s) 270, 374
FaqI GGGAC 2 cut(s) 136, 292
FatI CATG 2 cut(s) 268, 372
Fnu4HI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
Fsp4HI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
FspBI CTAG 2 cut(s) 93, 110
GlaI GCGC 1 cut(s) 119
GluI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
HaeII RGCGCY 1 cut(s) 121
HapII CCGG 1 cut(s) 47
HgaI GACGC 3 cut(s) 47, 89, 133
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 2 cut(s) 58, 144
Hin1II CATG 2 cut(s) 272, 376
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 1 cut(s) 365
HpaII CCGG 1 cut(s) 47
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 2 cut(s) 264, 316
Hpy188III TCNNGA 3 cut(s) 93, 153, 373
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 2 cut(s) 105, 215
HpyCH4V TGCA 2 cut(s) 162, 268
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 50, 112, 121, 165
HpyF3I CTNAG 1 cut(s) 308
Hsp92I GRCGYC 2 cut(s) 58, 144
Hsp92II CATG 2 cut(s) 272, 376
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 3 cut(s) 219, 246, 250
LmnI GCTCC 3 cut(s) 49, 111, 392
LpnPI CCDG 5 cut(s) 5, 26, 60, 134, 295
Lsp1109I GCAGC 4 cut(s) 35, 70, 127, 146
LweI GCATC 3 cut(s) 17, 98, 255
MaeI CTAG 2 cut(s) 93, 110
MalI GATC 3 cut(s) 221, 248, 252
MboI GATC 3 cut(s) 219, 246, 250
MboII GAAGA 2 cut(s) 236, 250
MluCI AATT 1 cut(s) 184
MnlI CCTC 3 cut(s) 30, 48, 149
Mph1103I ATGCAT 1 cut(s) 270
MseI TTAA 2 cut(s) 74, 415
MspA1I CMGCKG 2 cut(s) 26, 124
MspI CCGG 1 cut(s) 47
MwoI GCNNNNNNNGC 5 cut(s) 29, 50, 112, 121, 165
NdeII GATC 3 cut(s) 219, 246, 250
NlaIII CATG 2 cut(s) 272, 376
NlaIV GGNNCC 2 cut(s) 107, 280
NmeAIII GCCGAG 1 cut(s) 120
NsiI ATGCAT 1 cut(s) 270
NspI RCATGY 1 cut(s) 272
PagI TCATGA 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 19
PkrI GCNGC 8 cut(s) 25, 28, 31, 52, 85, 88, 117, 161
PspN4I GGNNCC 2 cut(s) 107, 280
PspPI GGNCC 2 cut(s) 279, 312
PstNI CAGNNNCTG 2 cut(s) 19, 83
RsaI GTAC 2 cut(s) 99, 176
RsaNI GTAC 2 cut(s) 98, 175
SaqAI TTAA 2 cut(s) 74, 415
SatI GCNGC 8 cut(s) 24, 27, 30, 51, 84, 87, 116, 160
Sau3AI GATC 3 cut(s) 219, 246, 250
Sau96I GGNCC 2 cut(s) 279, 312
SetI ASST 5 cut(s) 46, 141, 309, 314, 397
SfaNI GCATC 3 cut(s) 17, 98, 255
SinI GGWCC 2 cut(s) 279, 312
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 1 cut(s) 184
SsiI CCGC 6 cut(s) 26, 29, 32, 50, 86, 124
SspMI CTAG 2 cut(s) 93, 110
TaqI TCGA 3 cut(s) 210, 222, 249
TasI AATT 1 cut(s) 184
TauI GCSGC 4 cut(s) 29, 32, 53, 89
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 2 cut(s) 74, 415
Tru9I TTAA 2 cut(s) 74, 415
TseI GCWGC 4 cut(s) 23, 83, 115, 159
TspDTI ATGAA 1 cut(s) 361
Van91I CCANNNNNTGG 1 cut(s) 19
VpaK11BI GGWCC 2 cut(s) 279, 312
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 1 cut(s) 272
XspI CTAG 2 cut(s) 93, 110
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.