Rmu_sc0004220.1_g000001

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004220.1
OG0001719
Physical Location & Seq
Reverse (-)
1 .. 1114
1114 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 928 bp
atgatttctctcagtctctgtaaatcccggtcatctctcctctgtctgtcctggctatgcaccaattgctcaagaactcatggactcgcctcgtttgcactcagtcctcgctctaacttcatcactctcactcattgttaccgtggatactgctcttactcttctgggattaatagtaacaactctgagcttgaagagttatggagttcagaggagggtttagttcggtgctccggaaatcacgcccctttatctccaataggattcttggagcgagctgcaaaggcctacagggacagaacctctgtaatctatggctctctgaaatacacttgggctcaaacccatcaacgctgccttaaattagcttccgctctcactcggttgggaatttctcgcggtgatgtggtgagtgctccaatggctggggcaattctttgtacactaaatgctcggcttgattcagctatgatatctgttcttctttgtcattctgaagctaaaatcgtctttgtcgatcaccaattacttggaattgttgatggagcacttgaattaattgccgaaaaagctgtttcaaaaccgcctattgtagttttaattgctgattctgatgggtcatcatctccaaacagctctacttcagaaactgaaccaccttatgagtatgaaagtcttttagaaagtggaaatagtggatttgagattaggagaccaaaaagtgaatgggatcctatcagtgtaaattacacctctggcacaacatcaaggccgaaaggggttgtctataatcaccgcggcgcttatctcaattcactggccacagttttgctttgcggaataggctcaatgccggttaatctatggactgcgcctgtgtttcattgccatggatggtctctcatttggggtttgcctgctcagggtg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

309

Amino Acids

33.48

Weight (kDa)

7.54

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 90 - 304 5e-31 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 425
AccBSI CCGCTC 1 cut(s) 374
AccII CGCG 2 cut(s) 399, 798
AccIII TCCGGA 1 cut(s) 233
AciI CCGC 6 cut(s) 372, 399, 584, 796, 798, 837
AclWI GGATC 2 cut(s) 725, 738
AcoI YGGCCR 1 cut(s) 819
AcsI RAATTY 1 cut(s) 390
AcuI CTGAAG 2 cut(s) 516, 627
AfaI GTAC 1 cut(s) 442
AfiI CCNNNNNNNGG 2 cut(s) 425, 923
AgsI TTSAA 3 cut(s) 194, 554, 579
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 7 cut(s) 190, 278, 368, 467, 500, 572, 636
AluI AGCT 7 cut(s) 190, 278, 368, 467, 500, 572, 636
Alw21I GWGCWC 3 cut(s) 233, 418, 550
Alw26I GTCTC 3 cut(s) 20, 706, 903
AlwI GGATC 2 cut(s) 725, 738
AlwNI CAGNNNCTG 2 cut(s) 18, 650
Aor13HI TCCGGA 1 cut(s) 233
AoxI GGCC 3 cut(s) 285, 770, 819
ApeKI GCWGC 2 cut(s) 278, 354
ApoI RAATTY 1 cut(s) 390
AseI ATTAAT 2 cut(s) 171, 557
AspLEI GCGC 2 cut(s) 803, 874
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 4 cut(s) 413, 421, 512, 785
BalI TGGCCA 1 cut(s) 821
BamHI GGATCC 1 cut(s) 730
BanII GRGCYC 1 cut(s) 340
Bbv12I GWGCWC 3 cut(s) 233, 418, 550
BbvI GCAGC 2 cut(s) 265, 341
BccI CCATC 4 cut(s) 354, 536, 608, 888
BciT130I CCWGG 1 cut(s) 52
BciVI GTATCC 1 cut(s) 140
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 20, 706, 903
BfmI CTRYAG 1 cut(s) 289
BfoI RGCGCY 1 cut(s) 804
BfuI GTATCC 1 cut(s) 140
BisI GCNGC 3 cut(s) 279, 355, 799
BlsI GCNGC 3 cut(s) 280, 356, 800
Bme1390I CCNGG 2 cut(s) 28, 52
BmiI GGNNCC 1 cut(s) 732
BmrFI CCNGG 2 cut(s) 28, 52
Bpu10I CCTNAGC 1 cut(s) 921
BpuEI CTTGAG 1 cut(s) 55
BpuMI CCSGG 1 cut(s) 28
BsaI GGTCTC 2 cut(s) 706, 903
BsaJI CCNNGG 3 cut(s) 142, 796, 889
BsaWI WCCGGW 1 cut(s) 233
Bsc4I CCNNNNNNNGG 2 cut(s) 425, 923
Bse118I RCCGGY 1 cut(s) 853
Bse1I ACTGG 1 cut(s) 822
Bse3DI GCAATG 1 cut(s) 883
BseAI TCCGGA 1 cut(s) 233
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 3 cut(s) 142, 796, 889
BseGI GGATG 1 cut(s) 899
BseLI CCNNNNNNNGG 2 cut(s) 425, 923
BseMI GCAATG 1 cut(s) 883
BseMII CTCAG 3 cut(s) 25, 115, 177
BseNI ACTGG 1 cut(s) 822
BseRI GAGGAG 2 cut(s) 29, 227
BseXI GCAGC 2 cut(s) 265, 341
BseYI CCCAGC 1 cut(s) 425
Bsh1236I CGCG 2 cut(s) 399, 798
BshFI GGCC 3 cut(s) 287, 772, 821
BsiHKAI GWGCWC 3 cut(s) 233, 418, 550
BsiSI CCGG 3 cut(s) 28, 234, 854
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 2 cut(s) 425, 923
BsmAI GTCTC 3 cut(s) 20, 706, 903
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 3 cut(s) 287, 772, 821
Bso31I GGTCTC 2 cut(s) 706, 903
Bsp1286I GDGCHC 4 cut(s) 233, 340, 418, 550
Bsp13I TCCGGA 1 cut(s) 233
Bsp1407I TGTACA 1 cut(s) 440
Bsp143I GATC 2 cut(s) 517, 730
Bsp19I CCATGG 1 cut(s) 889
BspACI CCGC 6 cut(s) 372, 399, 584, 796, 798, 837
BspANI GGCC 3 cut(s) 287, 772, 821
BspCNI CTCAG 3 cut(s) 24, 114, 178
BspEI TCCGGA 1 cut(s) 233
BspFNI CGCG 2 cut(s) 399, 798
BspLI GGNNCC 1 cut(s) 732
BspPI GGATC 2 cut(s) 725, 738
BspTNI GGTCTC 2 cut(s) 706, 903
BsrBI CCGCTC 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 883
BsrFI RCCGGY 1 cut(s) 853
BsrGI TGTACA 1 cut(s) 440
BsrI ACTGG 1 cut(s) 822
BssAI RCCGGY 1 cut(s) 853
BssECI CCNNGG 3 cut(s) 142, 796, 889
BssMI GATC 2 cut(s) 517, 730
BssT1I CCWWGG 1 cut(s) 889
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 2 cut(s) 143, 826
Bst6I CTCTTC 2 cut(s) 166, 189
BstAPI GCANNNNNTGC 1 cut(s) 66
BstAUI TGTACA 1 cut(s) 440
BstC8I GCNNGC 2 cut(s) 276, 918
BstDEI CTNAG 4 cut(s) 11, 101, 186, 921
BstDSI CCRYGG 3 cut(s) 142, 796, 889
BstF5I GGATG 1 cut(s) 899
BstFNI CGCG 2 cut(s) 399, 798
BstH2I RGCGCY 1 cut(s) 804
BstHHI GCGC 2 cut(s) 803, 874
BstKTI GATC 2 cut(s) 520, 733
BstMAI GTCTC 3 cut(s) 20, 706, 903
BstMBI GATC 2 cut(s) 517, 730
BstMWI GCNNNNNNNGC 6 cut(s) 66, 95, 284, 422, 569, 843
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 26, 50
BstSFI CTRYAG 1 cut(s) 289
BstUI CGCG 2 cut(s) 399, 798
BstV1I GCAGC 2 cut(s) 265, 341
BstX2I RGATCY 1 cut(s) 730
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 1 cut(s) 730
BsuI GTATCC 1 cut(s) 140
BsuRI GGCC 3 cut(s) 287, 772, 821
BtgI CCRYGG 3 cut(s) 142, 796, 889
BtsCI GGATG 1 cut(s) 899
BtsIMutI CAGTG 2 cut(s) 745, 815
Cac8I GCNNGC 2 cut(s) 276, 918
CaiI CAGNNNCTG 2 cut(s) 18, 650
CfoI GCGC 2 cut(s) 803, 874
Cfr10I RCCGGY 1 cut(s) 853
Cfr42I CCGCGG 1 cut(s) 799
Csp6I GTAC 1 cut(s) 441
CviAII CATG 2 cut(s) 80, 890
CviQI GTAC 1 cut(s) 441
DdeI CTNAG 4 cut(s) 11, 101, 186, 921
DpnI GATC 2 cut(s) 519, 732
DpnII GATC 2 cut(s) 517, 730
EaeI YGGCCR 1 cut(s) 819
Eam1104I CTCTTC 2 cut(s) 166, 189
EarI CTCTTC 2 cut(s) 166, 189
Eco130I CCWWGG 1 cut(s) 889
Eco147I AGGCCT 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 340
Eco31I GGTCTC 2 cut(s) 706, 903
Eco32I GATATC 1 cut(s) 474
Eco57I CTGAAG 2 cut(s) 516, 627
EcoRII CCWGG 1 cut(s) 50
EcoRV GATATC 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 889
EcoT38I GRGCYC 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 889
FaeI CATG 2 cut(s) 83, 893
FaqI GGGAC 1 cut(s) 308
FatI CATG 2 cut(s) 79, 889
Fnu4HI GCNGC 3 cut(s) 279, 355, 799
FokI GGATG 1 cut(s) 906
FriOI GRGCYC 1 cut(s) 340
Fsp4HI GCNGC 3 cut(s) 279, 355, 799
GlaI GCGC 2 cut(s) 802, 873
GluI GCNGC 3 cut(s) 279, 355, 799
GsaI CCCAGC 1 cut(s) 429
HaeII RGCGCY 1 cut(s) 804
HaeIII GGCC 3 cut(s) 287, 772, 821
HapII CCGG 3 cut(s) 28, 234, 854
HhaI GCGC 2 cut(s) 803, 874
Hin1II CATG 2 cut(s) 83, 893
Hin6I GCGC 2 cut(s) 801, 872
HinP1I GCGC 2 cut(s) 801, 872
HinfI GANTC 4 cut(s) 84, 264, 461, 608
HpaII CCGG 3 cut(s) 28, 234, 854
HphI GGTGA 4 cut(s) 413, 421, 512, 785
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 6 cut(s) 187, 211, 324, 496, 613, 646
Hpy188III TCNNGA 2 cut(s) 72, 234
Hpy8I GTNNAC 1 cut(s) 443
HpyCH4III ACNGT 2 cut(s) 143, 826
HpyCH4V TGCA 3 cut(s) 60, 98, 281
HpyF10VI GCNNNNNNNGC 6 cut(s) 66, 95, 284, 422, 569, 843
HpyF3I CTNAG 4 cut(s) 11, 101, 186, 921
Hsp92II CATG 2 cut(s) 83, 893
HspAI GCGC 2 cut(s) 801, 872
Kpn2I TCCGGA 1 cut(s) 233
KspI CCGCGG 1 cut(s) 799
Kzo9I GATC 2 cut(s) 517, 730
LmnI GCTCC 4 cut(s) 236, 271, 421, 545
Lsp1109I GCAGC 2 cut(s) 265, 341
MaeIII GTNAC 2 cut(s) 137, 176
MalI GATC 2 cut(s) 519, 732
MbiI CCGCTC 1 cut(s) 374
MboI GATC 2 cut(s) 517, 730
MboII GAAGA 3 cut(s) 153, 206, 473
MfeI CAATTG 1 cut(s) 64
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 4 cut(s) 233, 340, 418, 550
MlsI TGGCCA 1 cut(s) 821
MluNI TGGCCA 1 cut(s) 821
MlyI GAGTC 1 cut(s) 78
MnlI CCTC 7 cut(s) 50, 100, 117, 205, 208, 313, 763
Mox20I TGGCCA 1 cut(s) 821
MroI TCCGGA 1 cut(s) 233
MscI TGGCCA 1 cut(s) 821
MseI TTAA 5 cut(s) 171, 360, 557, 599, 858
MslI CAYNNNNRTG 1 cut(s) 888
Msp20I TGGCCA 1 cut(s) 821
MspA1I CMGCKG 1 cut(s) 798
MspI CCGG 3 cut(s) 28, 234, 854
MspR9I CCNGG 2 cut(s) 28, 52
MunI CAATTG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 52
MvnI CGCG 2 cut(s) 399, 798
MwoI GCNNNNNNNGC 6 cut(s) 66, 95, 284, 422, 569, 843
NciI CCSGG 1 cut(s) 28
NcoI CCATGG 1 cut(s) 889
NdeII GATC 2 cut(s) 517, 730
NlaIII CATG 2 cut(s) 83, 893
NlaIV GGNNCC 1 cut(s) 732
NmeAIII GCCGAG 1 cut(s) 433
PceI AGGCCT 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 513
PfeI GAWTC 3 cut(s) 264, 461, 608
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 3 cut(s) 280, 356, 800
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PshBI ATTAAT 2 cut(s) 171, 557
Psp6I CCWGG 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 425
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 732
PstNI CAGNNNCTG 2 cut(s) 18, 650
PsuI RGATCY 1 cut(s) 730
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
RseI CAYNNNNRTG 1 cut(s) 888
SacII CCGCGG 1 cut(s) 799
SaqAI TTAA 5 cut(s) 171, 360, 557, 599, 858
SatI GCNGC 3 cut(s) 279, 355, 799
Sau3AI GATC 2 cut(s) 517, 730
SchI GAGTC 1 cut(s) 78
ScrFI CCNGG 2 cut(s) 28, 52
SduI GDGCHC 4 cut(s) 233, 340, 418, 550
SfcI CTRYAG 1 cut(s) 289
Sfr303I CCGCGG 1 cut(s) 799
SgrBI CCGCGG 1 cut(s) 799
SmiMI CAYNNNNRTG 1 cut(s) 888
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
SseBI AGGCCT 1 cut(s) 287
SsiI CCGC 6 cut(s) 372, 399, 584, 796, 798, 837
StuI AGGCCT 1 cut(s) 287
StyD4I CCNGG 2 cut(s) 26, 50
StyI CCWWGG 1 cut(s) 889
TaaI ACNGT 2 cut(s) 143, 826
TaqI TCGA 1 cut(s) 516
TatI WGTACW 1 cut(s) 440
TauI GCSGC 1 cut(s) 801
TfiI GAWTC 3 cut(s) 264, 461, 608
Tru1I TTAA 5 cut(s) 171, 360, 557, 599, 858
Tru9I TTAA 5 cut(s) 171, 360, 557, 599, 858
TscAI CASTG 2 cut(s) 745, 822
TseI GCWGC 2 cut(s) 278, 354
TspDTI ATGAA 3 cut(s) 109, 684, 872
TspRI CASTG 2 cut(s) 745, 822
Van91I CCANNNNNTGG 1 cut(s) 425
VspI ATTAAT 2 cut(s) 171, 557
XapI RAATTY 1 cut(s) 390
XcmI CCANNNNNNNNNTGG 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.