Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004467.1

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Reverse (-)

95431 .. 101550

Gene Structure 6120 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5163 bp

atgaggggatcaaagccggccggaatgtcgtggccgcaggctgagcagcgacgattttatccgcaaccagggtcccacccacgcgccgccgtcccgcgacggccggtgcctcgaattcagcggccgaatttcatcgtttatcttctgtccgacaaacgggaccaccgggggtcggacatcgaggcggtgatcaaccagtgcagggcgaagccggagaatttccgggtgagtccggagaacaacacagtcgcgtcgatgttctacgcgcagtggtacgacgcgctcgaagcgatggtctggctgtgggagtcgcggctcgaccgggttcacaatttcacgcccaagcttgacgccaacgactccgtctccacgccgtccgatagcgtggagctggaggaccgcctgagaaccttgttcgccggccgaattaagcagttaatcgacggcgacgaggtgaagaagtgtgaagcaaagcgccaaattttggccagagagtacgaccgagttcgtaagctggccaagaagcctcagaggaattgggaggacttaccggagaaggagaagaggtgcagagttgagcttgaattggttgagagtaggatcagagagttcaggtctgctatgaactgcttgcttgctcgtgttgaagggaaagagttggaggagtatggtgaagaaggcgtgaagctgtttaaattcggcgaaatttggcattggagtcgaattcagaggttgatggtgagggagtgtcgccgactcgatgagggattgcccatttatgctcataggcggcaaattcttgagcagattactaatcagcaggtcatggtgttgattggagagactggttcggggaagagtacacaattggttcagtttcttgctgattctggaatcgctgctgataagtccattgtatgcactcagcctcgcaagattgctgccaattcattggcaaagagagtcaaggaagaaagtagtgggtgttactatggagaaaacacagttaccagttatcagacatcgtctggactgcagttgacttctaaggtaacatatatgacagaccactgtttactgcagtactacatgaaagatagaaatatgaccatgatttcttgcatcatagttgatgaggctcatgaaaggaccttgagtacagatctccttttagcattgatcaaagatttactgggtcgaaggcatcagttaaggcttataataatgtctgcaacagctgatgcagaagtgctttcacattattttttcaactgtaaaatctttcatgtagtggggaggaactttccagttgatgtaagatatgctccctctttcactgacggaactgccagtaatgttgcttcttatgtttctgatgtgatgagggtagcaagagagatccacaagaatgagaaagaagggacaattcttgcatttttgacttctcagatggaagtcgaatgggtctgcgagaagtttataacacctggtgcaattgcattgccattgcatgggaaactttcttttgaagaacaatctaatgtttttcaaaatcaccctgggaaaagaaaaattatattcgccacaaatctggcggagacatccctgacaattcctggggtaaagtatgtgattgattctggcatggtcaaagaaagcaaatttgaacctggaagtggtatgaatgttctcagagtttgcaggatcagccagagttctgctaagcaaagaactgggcgtgctggaagaacggaacctggaatatgttatagactctactcagaatatgattttcaggcgatgcctccatgtcaggagcccgagatccgtagagttcatcttggtgtagcagttctgaggattctggctttgggtgtcaagaatttaaaagagtttgaattcattgatgcaccttgttctgaagctatagacatggcaatgaggaatcttgttcagttaggagctgttaagcaaaacaatgatgtctatgaactaactcaagagggtcgcgacttggttaaattgggggttgagcctaggcttggtaaattaattcttggctgctatcaccataacttgcgtaaggagggccttgttcttgcggctgtaatggcaaatgcaagtagcatattttgcagagttggtaatgacgaggagaaacttaggtcagactgcttcaaggtgcaattttgccatcgtgatggtgacctctttacccttctatcagtgtacaagatatgggaagctctgcctcgagagaggagaaacacatggtgttgggaaaacagcatcaatgccaaaactatgaggagatgccaggacactgtatcagaattggagttttgccttaaacatgagctcaataggataattccttgtagctggcgttggaatgcgcatgtttcaactgattgtgatatatacttgaaaaaggttatactttcttccctggcagaaaatgtagcaatgttctcaggatatgatcaagttggttatgaggtggcattaactgggcagcatgttcggctgcacccttcttgttcattgctggtattcggccagaaacctagttgggttgtttttggtgaactcctctcaagttctaatcaatatttggcctgtgtcactaccattgattttaactctttgtctacccttgatccccctcctgtgtttgatgtgtccaagatggaaggtcgaaagttgcaactgaaagtgttgactggttttggcagttgtttgcttaaaagattttgtgggaagggcaacaattatctgcatcatcttgtatctcgtgtcaggacaatttgcaatgatgaacttatcagcattaaagtcgattattgtcagaatgagattatggtgtttgctaccccacataacatggatagggttataaaccttgtatccgatgcattggagtgtgaaaaaagatggctgcgtaatgaatgcttggagaaatgcttgtatcatggttctggtggcttgcctcctgtagcattgtttggggctggtgcagagatcaagcatctggagcttcataaaaggtgtttgactgttgatgtatttcattcaaaactggatgacatggatgataaggagtttcttactgagcttgaagagtctgcctctggtagtatctgtggtcatcataagttccccggcactgggcaggaaaatgttgacaaaggaaagggggccaggttgacatttcttactcctgatgctgctcagaaagctgttgaattaaatgattctgagtttaatggttctatactgaaggttgttccttcacaggttggaggtgatcataaggtctatccactccttgctgttagagctaaaattttatggcctcgcaggcaaagccatgggtttgcaattgttaaatgtgacatggatgatatttaccccatgcttgctgatttttctaaccttgtagttggaggaagaaatattcgttgtcagcctagcaaaagatacacgtccgactctgttgtgattagtggactaaacagagatctttctgagaaagaaatccttgatgtgttaaggactgctactagtaggacaatcctggatttttttctggtgagaggggatgctgtagaaaatccgccatgtggtgcatgtgaagaagcactgctaaaagaaatatcaccctatatgcctaaacggtactcccatagtagctgcactgttcaggttttccagcctgaaccaaagaatcatttcatgagagctttgataacatttgacggaagattacacttggaggcagcaaaggctttagaacaattggaagggaaagtattgcccggcttccttccatggcaaaagatggagtgtcagcagttatttcatagctctctgtcctgccctggccctgtttatcgtgtaataaagaagcagctagatcctttacttgaaagttttacgcatctaaaaggtgtagaatgtaatctcgaggagtatcctaatggttcctgcagagttaagatatcagccaatgctacaaaaacgattgctgatctgaggagacgtgtggaggagcttgtgagggggaagactatagaccacccaagcctcactgcaactgttttgcagcttctgttttccagagatggcaccagtctaatgaactcgttacagcgagagacaggaacatatatcatttttgacaggcacaaactcaatgtacaggtctttggtgaagcacataaagttgacatggtcaagcagaaattggttgaatcccttttaaacctccatgaaagcaaggtgcttgagatccgccttcaaggtaatgctttacctcctgagttgatgaaagaggttgttagaaggtttgggccagacctccgggggctcaaagagaaggtaccaggggctgattttactctaaatgtacgccgtcaatccattctcatctacggaagcaaggagttgaagcaaaaagttgaagagattattgatgatactgcacatatggctggtagtttgactaagaggataaatagggaagctgattgtccaatctgcttgtgtgatgttgaagacggttaccggctggaggactgtggtcacttgttttgtcgttcatgtttggtggagcagtgcgagtctgcaatcaataatcatgatagctttccattatgctgcactcatgagggttgcaggtctccaattttgatcacagatctcagatctcttttatcaattgagaagctggaagatctctttagggcttcggtggggtcatatgttgcttcaagttgtggcacctacaggttttgcccatctcccgactgcgcttcagtctatcaggtagcggctcctggaacagaaggtgaaccatttgtttgtggtgcatgttatgcggagacgtgtaccatgtgccatttagaacatcatccttacatgtcatgcaagcagtacaagaatttcaaggaagatccggattcttcattgaaggagtggtgcaaaggaaaagaacatgtgaagagctgcccggtttgcagatatacaattgagaagatagatgggtgcaaccatatagaatgtcggtgcgggaagcatatttgctgggtttgtttggcctattatggaagcagcgatgaatgttatggccacttgagaagtgttcacctggcaatcatttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1720

Amino Acids

194.94

Weight (kDa)

7.92

Isoelectric Point (pI)

47.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	263 - 416	5.8e-10	DEAD/DEAH box helicase
AAA_19	PF13245	264 - 406	3.7e-06	AAA domain
AAA_22	PF13401	272 - 409	5.4e-06	AAA domain
Helicase_C	PF00271	464 - 584	1.4e-14	Helicase conserved C-terminal domain
OB_NTP_bind	PF07717	809 - 883	5.4e-10	Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11	PF24475	985 - 1099	1.1e-48	DEAH11-like, RNA-binding domain
RRM_DEAH11	PF24637	1119 - 1200	1.8e-35	DEAH11 RRM domain
KH_DEAH11_1st	PF24638	1289 - 1353	4e-31	DEAH11 eukaryotic type KH-domain
KH_DEAH11_2nd	PF24641	1354 - 1427	8.1e-36	DEAH11 eukaryotic type KH-domain
KH_DEAH11	PF24471	1429 - 1496	6.5e-29	DEAH11-like, KH-domain
IBR	PF01485	1587 - 1643	3.7e-07	IBR domain, a half RING-finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1220, 1481, 2897
Acc16I	TGCGCA	1 cut(s)	2397
Acc36I	ACCTGC	2 cut(s)	811, 4668
Acc65I	GGTACC	1 cut(s)	4381
AccB1I	GGYRCC	4 cut(s)	106, 4127, 4381, 4781
AccB7I	CCANNNNNTGG	2 cut(s)	484, 1511
AccI	GTMKAC	1 cut(s)	2651
AccII	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
AccIII	TCCGGA	2 cut(s)	232, 4957
AclWI	GGATC	9 cut(s)	16, 608, 1393, 1712, 1819, 2654, 3911, 4285, 4949
AcoI	YGGCCR	9 cut(s)	18, 32, 101, 122, 421, 486, 516, 2557, 5128
AcuI	CTGAAG	3 cut(s)	1941, 3299, 4800
AcyI	GRCGYC	1 cut(s)	351
AdeI	CACNNNGTG	2 cut(s)	1490, 2274
AflIII	ACRYGT	5 cut(s)	3483, 4042, 4886, 4920, 4996
AhlI	ACTAGT	1 cut(s)	3563
AjiI	CACGTC	3 cut(s)	3486, 4043, 4887
Alw21I	GWGCWC	1 cut(s)	2361
Alw26I	GTCTC	7 cut(s)	370, 836, 1592, 4033, 4151, 4686, 4877
AlwI	GGATC	9 cut(s)	16, 608, 1393, 1712, 1819, 2654, 3911, 4285, 4949
AlwNI	CAGNNNCTG	2 cut(s)	4111, 4391
Ama87I	CYCGRG	3 cut(s)	1820, 2253, 3965
Aor13HI	TCCGGA	2 cut(s)	232, 4957
ArsI	GACNNNNNNTTYG	2 cut(s)	279, 311
AseI	ATTAAT	1 cut(s)	2051
Asp700I	GAANNNNTTC	1 cut(s)	3731
Asp718I	GGTACC	1 cut(s)	4381
AspA2I	CCTAGG	1 cut(s)	2036
AspLEI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4814
AspS9I	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
AsuC2I	CCSGG	7 cut(s)	167, 224, 323, 3162, 3819, 4364, 5012
AvaI	CYCGRG	3 cut(s)	1820, 2253, 3965
AvaII	GGWCC	4 cut(s)	72, 160, 397, 1149
AvrII	CCTAGG	1 cut(s)	2036
BalI	TGGCCA	3 cut(s)	488, 518, 5130
BanI	GGYRCC	4 cut(s)	106, 4127, 4381, 4781
BanII	GRGCYC	3 cut(s)	1821, 2361, 4371
BarI	GAAGNNNNNNTAC	2 cut(s)	1345, 1377
BauI	CACGAG	2 cut(s)	639, 2793
BbsI	GAAGAC	2 cut(s)	4073, 4563
Bbv12I	GWGCWC	1 cut(s)	2361
BceAI	ACGGC	5 cut(s)	74, 116, 358, 460, 4398
BcgI	CGANNNNNNTGC	6 cut(s)	701, 735, 2504, 2538, 2818, 2852
BciVI	GTATCC	2 cut(s)	2917, 3984
BclI	TGATCA	5 cut(s)	189, 1179, 2482, 3307, 4692
BcnI	CCSGG	7 cut(s)	167, 224, 323, 3162, 3819, 4364, 5012
BcoDI	GTCTC	7 cut(s)	370, 836, 1592, 4033, 4151, 4686, 4877
BcuI	ACTAGT	1 cut(s)	3563
BfaI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
BfmI	CTRYAG	8 cut(s)	1034, 1079, 1926, 2994, 3606, 3988, 4071, 4786
BfoI	RGCGCY	1 cut(s)	478
BfuAI	ACCTGC	2 cut(s)	811, 4668
BfuI	GTATCC	2 cut(s)	2917, 3984
BglI	GCCNNNNNGGC	1 cut(s)	3361
BglII	AGATCT	5 cut(s)	1162, 3520, 4699, 4706, 4735
BlnI	CCTAGG	1 cut(s)	2036
BlpI	GCTNAGC	2 cut(s)	42, 1722
BmcAI	AGTACT	1 cut(s)	1085
Bme18I	GGWCC	4 cut(s)	72, 160, 397, 1149
BmeT110I	CYCGRG	3 cut(s)	1820, 2253, 3965
BmgBI	CACGTC	3 cut(s)	3486, 4043, 4887
BmgT120I	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
BmrI	ACTGGG	4 cut(s)	1202, 1743, 2520, 3177
BmuI	ACTGGG	4 cut(s)	1202, 1743, 2520, 3177
BoxI	GACNNNNGTC	1 cut(s)	4581
BpiI	GAAGAC	2 cut(s)	4073, 4563
BplI	GAGNNNNNCTC	2 cut(s)	3113, 3145
BpmI	CTGGAG	3 cut(s)	413, 3053, 4592
Bpu1102I	GCTNAGC	2 cut(s)	42, 1722
BpuEI	CTTGAG	6 cut(s)	821, 1174, 1983, 2581, 4307, 5155
BpuMI	CCSGG	7 cut(s)	167, 224, 323, 3162, 3819, 4364, 5012
BsaHI	GRCGYC	1 cut(s)	351
BsaI	GGTCTC	1 cut(s)	4686
BsaWI	WCCGGW	3 cut(s)	232, 550, 4957
BsaXI	ACNNNNNCTCC	4 cut(s)	350, 380, 551, 581
Bse118I	RCCGGY	4 cut(s)	16, 103, 419, 4566
Bse3DI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BseAI	TCCGGA	2 cut(s)	232, 4957
BseGI	GGATG	6 cut(s)	1601, 3088, 3097, 3406, 3607, 4912
BseMI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BseRI	GAGGAG	8 cut(s)	677, 2168, 2275, 2323, 2582, 3983, 4051, 4064
BseX3I	CGGCCG	4 cut(s)	18, 101, 122, 421
BseYI	CCCAGC	1 cut(s)	5085
BsgI	GTGCAG	7 cut(s)	220, 589, 2513, 3036, 3679, 4467, 4645
Bsh1236I	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
Bsh1285I	CGRYCG	6 cut(s)	21, 104, 125, 322, 424, 502
BshNI	GGYRCC	4 cut(s)	106, 4127, 4381, 4781
BsiEI	CGRYCG	6 cut(s)	21, 104, 125, 322, 424, 502
BsiHKAI	GWGCWC	1 cut(s)	2361
BsiHKCI	CYCGRG	3 cut(s)	1820, 2253, 3965
BslFI	GGGAC	4 cut(s)	58, 77, 173, 1435
BsmAI	GTCTC	7 cut(s)	370, 836, 1592, 4033, 4151, 4686, 4877
BsmBI	CGTCTC	3 cut(s)	370, 4033, 4877
BsmFI	GGGAC	4 cut(s)	58, 77, 173, 1435
BsmI	GAATGC	2 cut(s)	2398, 2954
Bso31I	GGTCTC	1 cut(s)	4686
BsoBI	CYCGRG	3 cut(s)	1820, 2253, 3965
Bsp1286I	GDGCHC	3 cut(s)	1821, 2361, 4371
Bsp13I	TCCGGA	2 cut(s)	232, 4957
Bsp1407I	TGTACA	2 cut(s)	2229, 4198
Bsp1720I	GCTNAGC	2 cut(s)	42, 1722
Bsp19I	CCATGG	2 cut(s)	3370, 3830
Bsp68I	TCGCGA	1 cut(s)	2010
BspEI	TCCGGA	2 cut(s)	232, 4957
BspFNI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
BspHI	TCATGA	4 cut(s)	1141, 3735, 4639, 4666
BspMAI	CTGCAG	3 cut(s)	1038, 1083, 3992
BspMI	ACCTGC	2 cut(s)	811, 4668
BspPI	GGATC	9 cut(s)	16, 608, 1393, 1712, 1819, 2654, 3911, 4285, 4949
BspQI	GCTCTTC	1 cut(s)	4997
BspT107I	GGYRCC	4 cut(s)	106, 4127, 4381, 4781
BspTNI	GGTCTC	1 cut(s)	4686
BsrDI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BsrFI	RCCGGY	4 cut(s)	16, 103, 419, 4566
BsrGI	TGTACA	2 cut(s)	2229, 4198
BssAI	RCCGGY	4 cut(s)	16, 103, 419, 4566
BssNI	GRCGYC	1 cut(s)	351
BssSI	CACGAG	2 cut(s)	639, 2793
BssT1I	CCWWGG	3 cut(s)	2036, 3370, 3830
Bst2BI	CACGAG	2 cut(s)	639, 2793
Bst6I	CTCTTC	5 cut(s)	557, 851, 3114, 4458, 4997
BstACI	GRCGYC	1 cut(s)	351
BstAPI	GCANNNNNTGC	2 cut(s)	2133, 4877
BstAUI	TGTACA	2 cut(s)	2229, 4198
BstDSI	CCRYGG	2 cut(s)	3370, 3830
BstEII	GGTNACC	2 cut(s)	2204, 4562
BstF5I	GGATG	6 cut(s)	1601, 3088, 3097, 3406, 3607, 4912
BstFNI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
BstH2I	RGCGCY	1 cut(s)	478
BstHHI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4814
BstMAI	GTCTC	7 cut(s)	370, 836, 1592, 4033, 4151, 4686, 4877
BstMCI	CGRYCG	6 cut(s)	21, 104, 125, 322, 424, 502
BstNSI	RCATGY	6 cut(s)	2402, 2522, 3633, 4875, 4924, 5000
BstPAI	GACNNNNGTC	1 cut(s)	4581
BstPI	GGTNACC	2 cut(s)	2204, 4562
BstSFI	CTRYAG	8 cut(s)	1034, 1079, 1926, 2994, 3606, 3988, 4071, 4786
BstUI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
BstV2I	GAAGAC	2 cut(s)	4073, 4563
BstXI	CCANNNNNNTGG	3 cut(s)	952, 1591, 2201
BstZI	CGGCCG	4 cut(s)	18, 101, 122, 421
BsuI	GTATCC	2 cut(s)	2917, 3984
BtgI	CCRYGG	2 cut(s)	3370, 3830
BtgZI	GCGATG	3 cut(s)	305, 1814, 5130
BtrI	CACGTC	3 cut(s)	3486, 4043, 4887
BtsCI	GGATG	6 cut(s)	1601, 3088, 3097, 3406, 3607, 4912
BtsI	GCAGTG	4 cut(s)	275, 3641, 4089, 4622
BtuMI	TCGCGA	1 cut(s)	2010
BveI	ACCTGC	2 cut(s)	811, 4668
CaiI	CAGNNNCTG	2 cut(s)	4111, 4391
CciI	TCATGA	4 cut(s)	1141, 3735, 4639, 4666
CfoI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4814
Cfr10I	RCCGGY	4 cut(s)	16, 103, 419, 4566
Cfr13I	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
CseI	GACGC	3 cut(s)	240, 287, 359
CsiI	ACCWGGT	1 cut(s)	1486
DraI	TTTAAA	3 cut(s)	694, 1887, 4263
DraIII	CACNNNGTG	2 cut(s)	1490, 2274
EaeI	YGGCCR	9 cut(s)	18, 32, 101, 122, 421, 486, 516, 2557, 5128
EagI	CGGCCG	4 cut(s)	18, 101, 122, 421
Eam1104I	CTCTTC	5 cut(s)	557, 851, 3114, 4458, 4997
EarI	CTCTTC	5 cut(s)	557, 851, 3114, 4458, 4997
EciI	GGCGGA	3 cut(s)	1610, 3606, 4283
Ecl136II	GAGCTC	1 cut(s)	2359
EclXI	CGGCCG	4 cut(s)	18, 101, 122, 421
Eco130I	CCWWGG	3 cut(s)	2036, 3370, 3830
Eco24I	GRGCYC	3 cut(s)	1821, 2361, 4371
Eco31I	GGTCTC	1 cut(s)	4686
Eco32I	GATATC	1 cut(s)	4002
Eco47I	GGWCC	4 cut(s)	72, 160, 397, 1149
Eco52I	CGGCCG	4 cut(s)	18, 101, 122, 421
Eco53kI	GAGCTC	1 cut(s)	2359
Eco57I	CTGAAG	3 cut(s)	1941, 3299, 4800
Eco88I	CYCGRG	3 cut(s)	1820, 2253, 3965
Eco91I	GGTNACC	2 cut(s)	2204, 4562
EcoICRI	GAGCTC	1 cut(s)	2359
EcoO109I	RGGNCCY	3 cut(s)	72, 1149, 2089
EcoO65I	GGTNACC	2 cut(s)	2204, 4562
EcoRI	GAATTC	3 cut(s)	114, 723, 1898
EcoRV	GATATC	1 cut(s)	4002
EcoT14I	CCWWGG	3 cut(s)	2036, 3370, 3830
EcoT22I	ATGCAT	1 cut(s)	2917
EcoT38I	GRGCYC	3 cut(s)	1821, 2361, 4371
ErhI	CCWWGG	3 cut(s)	2036, 3370, 3830
Esp3I	CGTCTC	3 cut(s)	370, 4033, 4877
FalI	AAGNNNNNCTT	2 cut(s)	3525, 3557
FaqI	GGGAC	4 cut(s)	58, 77, 173, 1435
FauI	CCCGC	2 cut(s)	102, 5063
FauNDI	CATATG	2 cut(s)	4488, 4762
FbaI	TGATCA	5 cut(s)	189, 1179, 2482, 3307, 4692
FblI	GTMKAC	1 cut(s)	2651
FokI	GGATG	6 cut(s)	1588, 3095, 3104, 3413, 3614, 4899
FriOI	GRGCYC	3 cut(s)	1821, 2361, 4371
FspAI	RTGCGCAY	1 cut(s)	2397
FspBI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
FspI	TGCGCA	1 cut(s)	2397
GlaI	GCGC	6 cut(s)	85, 267, 282, 476, 2397, 4813
GsaI	CCCAGC	1 cut(s)	5089
GsuI	CTGGAG	3 cut(s)	413, 3053, 4592
HaeII	RGCGCY	1 cut(s)	478
HgaI	GACGC	3 cut(s)	240, 287, 359
HhaI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4814
Hin1I	GRCGYC	1 cut(s)	351
Hin6I	GCGC	6 cut(s)	84, 266, 281, 475, 2396, 4812
HinP1I	GCGC	6 cut(s)	84, 266, 281, 475, 2396, 4812
HincII	GTYRAC	5 cut(s)	1041, 2721, 3184, 3207, 4228
HindII	GTYRAC	5 cut(s)	1041, 2721, 3184, 3207, 4228
HindIII	AAGCTT	1 cut(s)	344
Hpy99I	CGWCG	6 cut(s)	54, 102, 256, 281, 446, 452
HpyCH4IV	ACGT	3 cut(s)	3485, 4042, 4886
HpySE526I	ACGT	3 cut(s)	3485, 4042, 4886
Hsp92I	GRCGYC	1 cut(s)	351
HspAI	GCGC	6 cut(s)	84, 266, 281, 475, 2396, 4812
KflI	GGGWCCC	1 cut(s)	72
Kpn2I	TCCGGA	2 cut(s)	232, 4957
KpnI	GGTACC	1 cut(s)	4385
KroI	GCCGGC	2 cut(s)	16, 419
KroNI	GCCGGC	2 cut(s)	18, 421
Ksp22I	TGATCA	5 cut(s)	189, 1179, 2482, 3307, 4692
LguI	GCTCTTC	1 cut(s)	4997
LmnI	GCTCC	8 cut(s)	388, 1330, 1816, 1961, 3034, 4051, 4612, 4840
MabI	ACCWGGT	1 cut(s)	1486
MaeI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
MaeII	ACGT	3 cut(s)	3485, 4042, 4886
MaeIII	GTNAC	9 cut(s)	986, 1006, 1051, 2204, 2623, 3392, 4146, 4562, 4583
MfeI	CAATTG	6 cut(s)	866, 1494, 3381, 3797, 4719, 5028
MhlI	GDGCHC	3 cut(s)	1821, 2361, 4371
MlsI	TGGCCA	3 cut(s)	488, 518, 5130
MluNI	TGGCCA	3 cut(s)	488, 518, 5130
MmeI	TCCRAC	7 cut(s)	153, 174, 639, 2369, 3280, 3424, 3513
Mox20I	TGGCCA	3 cut(s)	488, 518, 5130
Mph1103I	ATGCAT	1 cut(s)	2917
MroI	TCCGGA	2 cut(s)	232, 4957
MroNI	GCCGGC	2 cut(s)	16, 419
MroXI	GAANNNNTTC	1 cut(s)	3731
MscI	TGGCCA	3 cut(s)	488, 518, 5130
MslI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
Msp20I	TGGCCA	3 cut(s)	488, 518, 5130
MspA1I	CMGCKG	2 cut(s)	121, 1238
MunI	CAATTG	6 cut(s)	866, 1494, 3381, 3797, 4719, 5028
Mva1269I	GAATGC	2 cut(s)	2398, 2954
MvnI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
NaeI	GCCGGC	2 cut(s)	18, 421
NciI	CCSGG	7 cut(s)	167, 224, 323, 3162, 3819, 4364, 5012
NcoI	CCATGG	2 cut(s)	3370, 3830
NdeI	CATATG	2 cut(s)	4488, 4762
NgoMIV	GCCGGC	2 cut(s)	16, 419
NmuCI	GTSAC	4 cut(s)	2204, 2623, 3392, 4583
NruI	TCGCGA	1 cut(s)	2010
NsbI	TGCGCA	1 cut(s)	2397
NsiI	ATGCAT	1 cut(s)	2917
NspI	RCATGY	6 cut(s)	2402, 2522, 3633, 4875, 4924, 5000
PaeR7I	CTCGAG	2 cut(s)	2253, 3965
PagI	TCATGA	4 cut(s)	1141, 3735, 4639, 4666
PasI	CCCWGGG	1 cut(s)	1559
PciI	ACATGT	2 cut(s)	4920, 4996
PciSI	GCTCTTC	1 cut(s)	4997
PcsI	WCGNNNNNNNCGW	2 cut(s)	282, 447
PctI	GAATGC	2 cut(s)	2398, 2954
PdiI	GCCGGC	2 cut(s)	18, 421
PdmI	GAANNNNTTC	1 cut(s)	3731
PfeI	GAWTC	9 cut(s)	887, 894, 1637, 1861, 1945, 3254, 3727, 4253, 4961
PflFI	GACNNNGTC	3 cut(s)	362, 1024, 4232
PflMI	CCANNNNNTGG	2 cut(s)	484, 1511
PfoI	TCCNGGA	2 cut(s)	3576, 4837
PpuMI	RGGWCCY	2 cut(s)	72, 1149
PscI	ACATGT	2 cut(s)	4920, 4996
PshAI	GACNNNNGTC	1 cut(s)	4581
PshBI	ATTAAT	1 cut(s)	2051
PsiI	TTATAA	3 cut(s)	1220, 1481, 2897
Psp124BI	GAGCTC	1 cut(s)	2361
Psp5II	RGGWCCY	2 cut(s)	72, 1149
PspEI	GGTNACC	2 cut(s)	2204, 4562
PspFI	CCCAGC	1 cut(s)	5085
PspPI	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
PspPPI	RGGWCCY	2 cut(s)	72, 1149
PstI	CTGCAG	3 cut(s)	1038, 1083, 3992
PstNI	CAGNNNCTG	2 cut(s)	4111, 4391
PsyI	GACNNNGTC	3 cut(s)	362, 1024, 4232
PvuII	CAGCTG	1 cut(s)	1238
RruI	TCGCGA	1 cut(s)	2010
RseI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
SacI	GAGCTC	1 cut(s)	2361
SapI	GCTCTTC	1 cut(s)	4997
Sau96I	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
ScaI	AGTACT	1 cut(s)	1085
SduI	GDGCHC	3 cut(s)	1821, 2361, 4371
SexAI	ACCWGGT	1 cut(s)	1486
SfcI	CTRYAG	8 cut(s)	1034, 1079, 1926, 2994, 3606, 3988, 4071, 4786
Sfr274I	CTCGAG	2 cut(s)	2253, 3965
SinI	GGWCC	4 cut(s)	72, 160, 397, 1149
SlaI	CTCGAG	2 cut(s)	2253, 3965
SmiMI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
SmlI	CTYRAG	8 cut(s)	800, 1153, 1998, 2253, 2596, 3965, 4286, 5134
SmoI	CTYRAG	8 cut(s)	800, 1153, 1998, 2253, 2596, 3965, 4286, 5134
SpeI	ACTAGT	1 cut(s)	3563
SspI	AATATT	2 cut(s)	2612, 3457
SspMI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
SstI	GAGCTC	1 cut(s)	2361
StyI	CCWWGG	3 cut(s)	2036, 3370, 3830
TaiI	ACGT	3 cut(s)	3488, 4045, 4889
TaqII	GACCGA	1 cut(s)	516
TatI	WGTACW	6 cut(s)	860, 1083, 1157, 2229, 4198, 4935
TauI	GCSGC	7 cut(s)	37, 89, 124, 316, 793, 2105, 4835
TfiI	GAWTC	9 cut(s)	887, 894, 1637, 1861, 1945, 3254, 3727, 4253, 4961
TseFI	GTSAC	4 cut(s)	2204, 2623, 3392, 4583
Tsp45I	GTSAC	4 cut(s)	2204, 2623, 3392, 4583
TspGWI	ACGGA	6 cut(s)	352, 1356, 1766, 1817, 3774, 4449
Tth111I	GACNNNGTC	3 cut(s)	362, 1024, 4232
Van91I	CCANNNNNTGG	2 cut(s)	484, 1511
VpaK11BI	GGWCC	4 cut(s)	72, 160, 397, 1149
VspI	ATTAAT	1 cut(s)	2051
XceI	RCATGY	6 cut(s)	2402, 2522, 3633, 4875, 4924, 5000
XhoI	CTCGAG	2 cut(s)	2253, 3965
XmaJI	CCTAGG	1 cut(s)	2036
XmiI	GTMKAC	1 cut(s)	2651
XmnI	GAANNNNTTC	1 cut(s)	3731
XspI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
ZrmI	AGTACT	1 cut(s)	1085
Zsp2I	ATGCAT	1 cut(s)	2917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.