Rmu_sc0005139.1_g000010

5'-3' exodeoxyribonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005139.1
OG0008863
Physical Location & Seq
Reverse (-)
36560 .. 38325
1766 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 891 bp
atgaagatggggataggaaagggaagcgaattgttgggtgactttggagacaacgcagtaataagaggcatgcgcctcgacagactgagcagtgagggcattacgagagtaggggttgatgctactccctggcttgtgaccgttcaggcgttcaccatacacttgaggatcaccaaaagaaaaattatggactgtatgcatgcaaaacttttcatggagtattgcggtaagaggattaatgtactaaagccgaatggacttataccgactatggtctttgatggaaatgctgcaccattaaagacccacataaaggagctgaggaaagcgattagtgatgaagacaacaacaacatcaatacgaatgagatacgggatcctggtagtgatatcagtgacagtgatagtgatgttaatgtgaacagtggtagtgatcccgtagacaataagtcaccctataagtggccaaaacatagatacattcctgaaagatccgaagtagtcaagtggttgaccgaggagatggatgtaaagttctttgtggctccttatgaagccgatgcccaattgacacacttggcaagcaagaaaataatagacgccgtcatcaccaatgactcagaccttatagcattcggatgtaaaaaagatgtcgtggtgagaaacataaccaggttgttaggaccacgtttgaaggacgaatctgttcataatattgcaatgggtaatagaccttcgaagctcgaggacattgatgtagataatcggcctgaacagtatcatggaaagacgcaaggctcgtttaccaagttttctcatcagattgcaatgccacgtgtcacaaatctggaggttcagttagcaaggttgttagaagaaatgtgtagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03430 map03430
Pfam Domains
Protein Families
XPG_I XPG_N

Protein Analysis

296

Amino Acids

33.29

Weight (kDa)

6.72

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XPG_I PF00867 177 - 228 4.6e-14 XPG I-region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 441
AciI CCGC 1 cut(s) 225
AclWI GGATC 5 cut(s) 176, 371, 384, 428, 486
AcoI YGGCCR 1 cut(s) 464
AcvI CACGTG 1 cut(s) 836
AcyI GRCGYC 1 cut(s) 600
AfaI GTAC 1 cut(s) 243
AfiI CCNNNNNNNGG 2 cut(s) 313, 462
AflIII ACRYGT 1 cut(s) 835
AgsI TTSAA 1 cut(s) 694
AhdI GACNNNNNGTC 1 cut(s) 448
AjnI CCWGG 3 cut(s) 128, 379, 671
AloI GAACNNNNNNTCC 2 cut(s) 518, 550
AluBI AGCT 3 cut(s) 319, 742, 888
AluI AGCT 3 cut(s) 319, 742, 888
Alw26I GTCTC 1 cut(s) 42
AlwI GGATC 5 cut(s) 176, 371, 384, 428, 486
Ama87I CYCGRG 1 cut(s) 743
AoxI GGCC 2 cut(s) 464, 767
ApeKI GCWGC 1 cut(s) 290
AseI ATTAAT 1 cut(s) 237
Asp700I GAANNNNTTC 1 cut(s) 705
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 1 cut(s) 683
AsuHPI GGTGA 6 cut(s) 50, 145, 163, 444, 601, 670
AsuII TTCGAA 1 cut(s) 737
AvaI CYCGRG 1 cut(s) 743
AvaII GGWCC 1 cut(s) 683
BalI TGGCCA 1 cut(s) 466
BamHI GGATCC 1 cut(s) 376
BarI GAAGNNNNNNTAC 2 cut(s) 718, 750
BbrPI CACGTG 1 cut(s) 836
BbsI GAAGAC 1 cut(s) 348
BbvCI CCTCAGC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 277
BccI CCATC 2 cut(s) 275, 517
BceAI ACGGC 1 cut(s) 587
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 3 cut(s) 130, 381, 673
BcoDI GTCTC 1 cut(s) 42
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 3 cut(s) 130, 381, 673
Bme18I GGWCC 1 cut(s) 683
BmeRI GACNNNNNGTC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 743
BmgT120I GGNCC 1 cut(s) 683
BmiI GGNNCC 2 cut(s) 378, 546
BmrFI CCNGG 3 cut(s) 130, 381, 673
BmsI GCATC 2 cut(s) 109, 550
BoxI GACNNNNGTC 1 cut(s) 272
BpiI GAAGAC 1 cut(s) 348
BpmI CTGGAG 1 cut(s) 869
Bpu10I CCTNAGC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 737
BpuEI CTTGAG 1 cut(s) 184
BsaAI YACGTR 1 cut(s) 836
BsaHI GRCGYC 1 cut(s) 600
BsaJI CCNNGG 2 cut(s) 128, 516
BsaXI ACNNNNNCTCC 2 cut(s) 842, 872
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 462
Bse3DI GCAATG 2 cut(s) 726, 834
BseBI CCWGG 3 cut(s) 130, 381, 673
BseDI CCNNGG 2 cut(s) 128, 516
BseGI GGATG 2 cut(s) 532, 644
BseLI CCNNNNNNNGG 2 cut(s) 313, 462
BseMI GCAATG 2 cut(s) 726, 834
BseMII CTCAG 3 cut(s) 77, 311, 633
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 1 cut(s) 277
BsgI GTGCAG 1 cut(s) 276
BshFI GGCC 2 cut(s) 466, 769
BsiHKCI CYCGRG 1 cut(s) 743
BslI CCNNNNNNNGG 2 cut(s) 313, 462
BsmAI GTCTC 1 cut(s) 42
BsmI GAATGC 1 cut(s) 632
BsnI GGCC 2 cut(s) 466, 769
BsoBI CYCGRG 1 cut(s) 743
Bsp119I TTCGAA 1 cut(s) 737
Bsp143I GATC 4 cut(s) 168, 376, 433, 491
BspACI CCGC 1 cut(s) 225
BspANI GGCC 2 cut(s) 466, 769
BspCNI CTCAG 3 cut(s) 78, 312, 632
BspLI GGNNCC 2 cut(s) 378, 546
BspPI GGATC 5 cut(s) 176, 371, 384, 428, 486
BspT104I TTCGAA 1 cut(s) 737
BsrDI GCAATG 2 cut(s) 726, 834
BssECI CCNNGG 2 cut(s) 128, 516
BssMI GATC 4 cut(s) 168, 376, 433, 491
BssNI GRCGYC 1 cut(s) 600
Bst2UI CCWGG 3 cut(s) 130, 381, 673
Bst4CI ACNGT 5 cut(s) 142, 194, 401, 425, 777
BstACI GRCGYC 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 836
BstBI TTCGAA 1 cut(s) 737
BstC8I GCNNGC 3 cut(s) 71, 201, 583
BstDEI CTNAG 3 cut(s) 86, 320, 619
BstF5I GGATG 2 cut(s) 532, 644
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 4 cut(s) 171, 379, 436, 494
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 4 cut(s) 168, 376, 433, 491
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstNI CCWGG 3 cut(s) 130, 381, 673
BstNSI RCATGY 2 cut(s) 73, 203
BstPAI GACNNNNGTC 1 cut(s) 272
BstSCI CCNGG 3 cut(s) 128, 379, 671
BstV1I GCAGC 1 cut(s) 277
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 2 cut(s) 376, 491
BstYI RGATCY 2 cut(s) 376, 491
BsuRI GGCC 2 cut(s) 466, 769
BtsCI GGATG 2 cut(s) 532, 644
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 4 cut(s) 97, 400, 406, 430
Cac8I GCNNGC 3 cut(s) 71, 201, 583
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 1 cut(s) 683
CseI GACGC 2 cut(s) 608, 799
CsiI ACCWGGT 1 cut(s) 671
Csp6I GTAC 1 cut(s) 242
CviAII CATG 4 cut(s) 70, 200, 214, 782
CviQI GTAC 1 cut(s) 242
DdeI CTNAG 3 cut(s) 86, 320, 619
DpnI GATC 4 cut(s) 170, 378, 435, 493
DpnII GATC 4 cut(s) 168, 376, 433, 491
DriI GACNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 1 cut(s) 464
Eam1105I GACNNNNNGTC 1 cut(s) 448
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 683
Eco72I CACGTG 1 cut(s) 836
Eco88I CYCGRG 1 cut(s) 743
EcoRII CCWGG 3 cut(s) 128, 379, 671
EcoRV GATATC 1 cut(s) 391
EcoT22I ATGCAT 1 cut(s) 201
FaeI CATG 4 cut(s) 73, 203, 217, 785
FatI CATG 4 cut(s) 69, 199, 213, 781
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 2 cut(s) 539, 651
Fsp4HI GCNGC 1 cut(s) 291
GlaI GCGC 1 cut(s) 74
GluI GCNGC 1 cut(s) 291
GsuI CTGGAG 1 cut(s) 869
HaeIII GGCC 2 cut(s) 466, 769
HgaI GACGC 2 cut(s) 608, 799
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 1 cut(s) 600
Hin1II CATG 4 cut(s) 73, 203, 217, 785
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HinfI GANTC 2 cut(s) 617, 701
HphI GGTGA 6 cut(s) 50, 145, 163, 444, 601, 670
Hpy166II GTNNAC 5 cut(s) 153, 421, 442, 513, 804
Hpy188I TCNGA 4 cut(s) 496, 622, 638, 822
Hpy188III TCNNGA 2 cut(s) 485, 848
Hpy8I GTNNAC 5 cut(s) 153, 421, 442, 513, 804
HpyAV CCTTC 2 cut(s) 688, 744
HpyCH4III ACNGT 5 cut(s) 142, 194, 401, 425, 777
HpyCH4IV ACGT 2 cut(s) 688, 835
HpyCH4V TGCA 5 cut(s) 199, 203, 293, 719, 827
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
HpyF3I CTNAG 3 cut(s) 86, 320, 619
HpySE526I ACGT 2 cut(s) 688, 835
Hsp92I GRCGYC 1 cut(s) 600
Hsp92II CATG 4 cut(s) 73, 203, 217, 785
HspAI GCGC 1 cut(s) 73
Kzo9I GATC 4 cut(s) 168, 376, 433, 491
LmnI GCTCC 2 cut(s) 316, 550
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 2 cut(s) 109, 550
MabI ACCWGGT 1 cut(s) 671
MaeII ACGT 2 cut(s) 688, 835
MaeIII GTNAC 5 cut(s) 38, 136, 395, 450, 838
MalI GATC 4 cut(s) 170, 378, 435, 493
MboI GATC 4 cut(s) 168, 376, 433, 491
MboII GAAGA 3 cut(s) 16, 353, 887
MfeI CAATTG 1 cut(s) 566
MflI RGATCY 2 cut(s) 376, 491
MlsI TGGCCA 1 cut(s) 466
MluCI AATT 3 cut(s) 29, 183, 566
MluNI TGGCCA 1 cut(s) 466
MlyI GAGTC 1 cut(s) 611
MnlI CCTC 9 cut(s) 59, 86, 88, 159, 225, 315, 511, 739, 844
Mox20I TGGCCA 1 cut(s) 466
Mph1103I ATGCAT 1 cut(s) 201
MroXI GAANNNNTTC 1 cut(s) 705
MscI TGGCCA 1 cut(s) 466
MseI TTAA 3 cut(s) 237, 299, 414
MslI CAYNNNNRTG 1 cut(s) 637
Msp20I TGGCCA 1 cut(s) 466
MspR9I CCNGG 3 cut(s) 130, 381, 673
MunI CAATTG 1 cut(s) 566
Mva1269I GAATGC 1 cut(s) 632
MvaI CCWGG 3 cut(s) 130, 381, 673
MwoI GCNNNNNNNGC 1 cut(s) 96
NdeII GATC 4 cut(s) 168, 376, 433, 491
NlaIII CATG 4 cut(s) 73, 203, 217, 785
NlaIV GGNNCC 2 cut(s) 378, 546
NmuCI GTSAC 5 cut(s) 38, 136, 395, 450, 838
NsiI ATGCAT 1 cut(s) 201
NspI RCATGY 2 cut(s) 73, 203
NspV TTCGAA 1 cut(s) 737
PaeI GCATGC 2 cut(s) 73, 203
PaeR7I CTCGAG 1 cut(s) 743
PcsI WCGNNNNNNNCGW 1 cut(s) 797
PctI GAATGC 1 cut(s) 632
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 1 cut(s) 701
PflFI GACNNNGTC 1 cut(s) 602
PkrI GCNGC 1 cut(s) 292
PleI GAGTC 1 cut(s) 611
PmaCI CACGTG 1 cut(s) 836
PmlI CACGTG 1 cut(s) 836
PpsI GAGTC 1 cut(s) 611
Ppu21I YACGTR 1 cut(s) 836
PshAI GACNNNNGTC 1 cut(s) 272
PshBI ATTAAT 1 cut(s) 237
Psp6I CCWGG 3 cut(s) 128, 379, 671
PspCI CACGTG 1 cut(s) 836
PspGI CCWGG 3 cut(s) 128, 379, 671
PspN4I GGNNCC 2 cut(s) 378, 546
PspPI GGNCC 1 cut(s) 683
PspXI VCTCGAGB 1 cut(s) 743
PsuI RGATCY 2 cut(s) 376, 491
PsyI GACNNNGTC 1 cut(s) 602
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
RseI CAYNNNNRTG 1 cut(s) 637
SaqAI TTAA 3 cut(s) 237, 299, 414
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 4 cut(s) 168, 376, 433, 491
Sau96I GGNCC 1 cut(s) 683
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 3 cut(s) 130, 381, 673
SexAI ACCWGGT 1 cut(s) 671
SfaNI GCATC 2 cut(s) 109, 550
Sfr274I CTCGAG 1 cut(s) 743
SfuI TTCGAA 1 cut(s) 737
SinI GGWCC 1 cut(s) 683
SlaI CTCGAG 1 cut(s) 743
SmiMI CAYNNNNRTG 1 cut(s) 637
SmlI CTYRAG 2 cut(s) 163, 743
SmoI CTYRAG 2 cut(s) 163, 743
SphI GCATGC 2 cut(s) 73, 203
Sse9I AATT 3 cut(s) 29, 183, 566
SsiI CCGC 1 cut(s) 225
SspI AATATT 1 cut(s) 715
StyD4I CCNGG 3 cut(s) 128, 379, 671
TaaI ACNGT 5 cut(s) 142, 194, 401, 425, 777
TaiI ACGT 2 cut(s) 691, 838
TaqI TCGA 3 cut(s) 78, 737, 744
TaqII GACCGA 1 cut(s) 530
TasI AATT 3 cut(s) 29, 183, 566
TatI WGTACW 1 cut(s) 241
TfiI GAWTC 1 cut(s) 701
Tru1I TTAA 3 cut(s) 237, 299, 414
Tru9I TTAA 3 cut(s) 237, 299, 414
TscAI CASTG 4 cut(s) 97, 400, 406, 430
TseFI GTSAC 5 cut(s) 38, 136, 395, 450, 838
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 5 cut(s) 38, 136, 395, 450, 838
TspDTI ATGAA 5 cut(s) 17, 202, 354, 567, 698
TspRI CASTG 4 cut(s) 97, 400, 406, 430
Tth111I GACNNNGTC 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 683
VspI ATTAAT 1 cut(s) 237
XceI RCATGY 2 cut(s) 73, 203
XhoI CTCGAG 1 cut(s) 743
XmiI GTMKAC 1 cut(s) 441
XmnI GAANNNNTTC 1 cut(s) 705
Zsp2I ATGCAT 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.