Rroxscaffold_5G00387150

exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008863
Physical Location & Seq
Forward (+)
65864230 .. 65878834
14605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387150.1

Sequence Viewer

Length: 714 bp
ATGACGAAGACCATACCGCGGGTTCCATCATGGGAAGCCACCAAGCCTTTGAATATGCAGTTACCATCGGCATCTTTGCTGGCATATCCAGCAATAGGAGTTCGGCAAGACCAATACAGATTGAAGATGGGGGTAAAAAGAGCAGACGCATTGTTCGACCAGTTGGGACCAACGGTTCTAAAAGACATCCGTCTCGACCAATTGGACTTGAGCGGTAAAAAGGTGGGGATTGATGCCACCTTATGGCTTGGGACCGTCAAAAGTATGCTCAAGGCCAACCAAAATAAATTATCCTCAAGTACTGAAAAGTTTATCGTCAAGGTCCAAGAGAAGACAAAGATGTTGAACAAAAAGATAAAGGAGGGCAGCAGCAAGAAGAAGGAGGTGAATGTTGATCCTGATAGTAACATTGATCGTGAGTGTGATAATGACCGCTATAATGAACGTAAGAAAGTGAACTATAATAAGCCAGAAAAAATGTACATTCCTGATAGGAAAAGGTGGTTAACTAAAGAAGGTGTGGAGTTTATCGTGGCTCCTTACGAAGCTGATGCTCAATTAACATACTTGACAATAAAGGGTAAAATAGATGCAGTTATCACGGAGGACTCGGACCTCATTGCATTTGGTTGTCCACATGGCATACTCCCTGGTCATGAAACAAAAGATGGCAGTCCCTGCATTTTCCTCGTAACTTCTCAAAGAAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03430 map03430
Pfam Domains
Protein Families
XPG_I XPG_N

Protein Analysis

237

Amino Acids

26.8

Weight (kDa)

9.41

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XPG_I PF00867 173 - 213 1.5e-14 XPG I-region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 243
AccBSI CCGCTC 1 cut(s) 213
AccII CGCG 1 cut(s) 19
AciI CCGC 4 cut(s) 17, 19, 213, 433
AclWI GGATC 1 cut(s) 389
AfaI GTAC 2 cut(s) 301, 482
AfiI CCNNNNNNNGG 3 cut(s) 18, 95, 243
AgsI TTSAA 3 cut(s) 52, 124, 346
AjnI CCWGG 1 cut(s) 649
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
AluBI AGCT 1 cut(s) 548
AluI AGCT 1 cut(s) 548
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 1 cut(s) 389
AlwNI CAGNNNCTG 1 cut(s) 678
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 2 cut(s) 366, 369
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
AspS9I GGNCC 4 cut(s) 167, 252, 322, 613
AsuHPI GGTGA 1 cut(s) 397
AvaII GGWCC 4 cut(s) 167, 252, 322, 613
BbsI GAAGAC 2 cut(s) 14, 338
BbvI GCAGC 2 cut(s) 378, 381
BccI CCATC 4 cut(s) 34, 73, 121, 662
BcgI CGANNNNNNTGC 4 cut(s) 533, 567, 670, 704
BciT130I CCWGG 1 cut(s) 651
BcoDI GTCTC 1 cut(s) 197
BisI GCNGC 2 cut(s) 367, 370
BlsI GCNGC 2 cut(s) 368, 371
BmcAI AGTACT 1 cut(s) 301
Bme1390I CCNGG 1 cut(s) 651
Bme18I GGWCC 4 cut(s) 167, 252, 322, 613
BmgT120I GGNCC 4 cut(s) 167, 252, 322, 613
BmiI GGNNCC 4 cut(s) 24, 168, 253, 537
BmrFI CCNGG 1 cut(s) 651
BmsI GCATC 4 cut(s) 80, 223, 541, 580
BoxI GACNNNNGTC 1 cut(s) 189
BpiI GAAGAC 2 cut(s) 14, 338
BpuEI CTTGAG 3 cut(s) 229, 254, 280
BsaJI CCNNGG 2 cut(s) 17, 649
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 95, 243
Bse1I ACTGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 618
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 2 cut(s) 17, 649
BseGI GGATG 1 cut(s) 186
BseLI CCNNNNNNNGG 3 cut(s) 18, 95, 243
BseMI GCAATG 1 cut(s) 618
BseNI ACTGG 1 cut(s) 160
BseXI GCAGC 2 cut(s) 378, 381
Bsh1236I CGCG 1 cut(s) 19
BshFI GGCC 1 cut(s) 275
BslFI GGGAC 3 cut(s) 180, 265, 660
BslI CCNNNNNNNGG 3 cut(s) 18, 95, 243
BsmAI GTCTC 1 cut(s) 197
BsmBI CGTCTC 1 cut(s) 197
BsmFI GGGAC 3 cut(s) 180, 265, 660
BsnI GGCC 1 cut(s) 275
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 2 cut(s) 394, 412
BspACI CCGC 4 cut(s) 17, 19, 213, 433
BspANI GGCC 1 cut(s) 275
BspFNI CGCG 1 cut(s) 19
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 4 cut(s) 24, 168, 253, 537
BspPI GGATC 1 cut(s) 389
BsrBI CCGCTC 1 cut(s) 213
BsrDI GCAATG 1 cut(s) 618
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 2 cut(s) 17, 649
BssMI GATC 2 cut(s) 394, 412
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 2 cut(s) 175, 256
BstAPI GCANNNNNTGC 1 cut(s) 678
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 1 cut(s) 81
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 1 cut(s) 186
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 2 cut(s) 397, 415
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 2 cut(s) 394, 412
BstMWI GCNNNNNNNGC 2 cut(s) 89, 678
BstNI CCWGG 1 cut(s) 651
BstPAI GACNNNNGTC 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 649
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 2 cut(s) 378, 381
BstV2I GAAGAC 2 cut(s) 14, 338
BsuRI GGCC 1 cut(s) 275
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 81
CaiI CAGNNNCTG 1 cut(s) 678
CciI TCATGA 1 cut(s) 655
Cfr13I GGNCC 4 cut(s) 167, 252, 322, 613
Cfr42I CCGCGG 1 cut(s) 20
CseI GACGC 1 cut(s) 155
Csp6I GTAC 2 cut(s) 300, 481
CviAII CATG 3 cut(s) 30, 638, 656
CviJI RGCY 7 cut(s) 38, 46, 247, 275, 469, 536, 548
CviKI_1 RGCY 7 cut(s) 38, 46, 247, 275, 469, 536, 548
CviQI GTAC 2 cut(s) 300, 481
DpnI GATC 2 cut(s) 396, 414
DpnII GATC 2 cut(s) 394, 412
Eco47I GGWCC 4 cut(s) 167, 252, 322, 613
EcoRII CCWGG 1 cut(s) 649
Esp3I CGTCTC 1 cut(s) 197
FaeI CATG 3 cut(s) 33, 641, 659
FaqI GGGAC 3 cut(s) 180, 265, 660
FatI CATG 3 cut(s) 29, 637, 655
FauI CCCGC 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 367, 370
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 2 cut(s) 367, 370
GluI GCNGC 2 cut(s) 367, 370
HaeIII GGCC 1 cut(s) 275
HgaI GACGC 1 cut(s) 155
Hin1II CATG 3 cut(s) 33, 641, 659
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HinfI GANTC 1 cut(s) 608
HpaI GTTAAC 1 cut(s) 507
HphI GGTGA 1 cut(s) 397
Hpy166II GTNNAC 3 cut(s) 457, 507, 635
Hpy188I TCNGA 1 cut(s) 613
Hpy188III TCNNGA 5 cut(s) 194, 398, 416, 488, 656
Hpy8I GTNNAC 3 cut(s) 457, 507, 635
HpyAV CCTTC 2 cut(s) 373, 509
HpyCH4III ACNGT 2 cut(s) 175, 256
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 4 cut(s) 58, 593, 623, 681
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 678
HpySE526I ACGT 1 cut(s) 445
Hsp92II CATG 3 cut(s) 33, 641, 659
KspAI GTTAAC 1 cut(s) 507
KspI CCGCGG 1 cut(s) 20
Kzo9I GATC 2 cut(s) 394, 412
LmnI GCTCC 1 cut(s) 541
LpnPI CCDG 9 cut(s) 65, 102, 173, 411, 483, 501, 636, 663, 691
Lsp1109I GCAGC 2 cut(s) 378, 381
LweI GCATC 4 cut(s) 80, 223, 541, 580
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 3 cut(s) 60, 404, 691
MalI GATC 2 cut(s) 396, 414
MbiI CCGCTC 1 cut(s) 213
MboI GATC 2 cut(s) 394, 412
MboII GAAGA 4 cut(s) 19, 136, 343, 388
MfeI CAATTG 1 cut(s) 200
MluCI AATT 3 cut(s) 200, 287, 557
MlyI GAGTC 1 cut(s) 602
MnlI CCTC 6 cut(s) 304, 355, 376, 598, 626, 698
MseI TTAA 2 cut(s) 506, 560
MspA1I CMGCKG 1 cut(s) 19
MspR9I CCNGG 1 cut(s) 651
MunI CAATTG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 2 cut(s) 89, 678
NdeII GATC 2 cut(s) 394, 412
NlaIII CATG 3 cut(s) 33, 641, 659
NlaIV GGNNCC 4 cut(s) 24, 168, 253, 537
PagI TCATGA 1 cut(s) 655
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PflMI CCANNNNNTGG 1 cut(s) 243
PkrI GCNGC 2 cut(s) 368, 371
PleI GAGTC 1 cut(s) 602
PpsI GAGTC 1 cut(s) 602
PshAI GACNNNNGTC 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 649
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 4 cut(s) 24, 168, 253, 537
PspPI GGNCC 4 cut(s) 167, 252, 322, 613
PstNI CAGNNNCTG 1 cut(s) 678
RsaI GTAC 2 cut(s) 301, 482
RsaNI GTAC 2 cut(s) 300, 481
SacII CCGCGG 1 cut(s) 20
SaqAI TTAA 2 cut(s) 506, 560
SatI GCNGC 2 cut(s) 367, 370
Sau3AI GATC 2 cut(s) 394, 412
Sau96I GGNCC 4 cut(s) 167, 252, 322, 613
ScaI AGTACT 1 cut(s) 301
SchI GAGTC 1 cut(s) 602
ScrFI CCNGG 1 cut(s) 651
SetI ASST 9 cut(s) 225, 242, 324, 387, 448, 503, 520, 550, 618
SfaNI GCATC 4 cut(s) 80, 223, 541, 580
Sfr303I CCGCGG 1 cut(s) 20
SgrBI CCGCGG 1 cut(s) 20
SinI GGWCC 4 cut(s) 167, 252, 322, 613
SmlI CTYRAG 3 cut(s) 208, 269, 295
SmoI CTYRAG 3 cut(s) 208, 269, 295
Sse9I AATT 3 cut(s) 200, 287, 557
SsiI CCGC 4 cut(s) 17, 19, 213, 433
StyD4I CCNGG 1 cut(s) 649
TaaI ACNGT 2 cut(s) 175, 256
TaiI ACGT 1 cut(s) 448
TaqI TCGA 2 cut(s) 156, 195
TasI AATT 3 cut(s) 200, 287, 557
TatI WGTACW 2 cut(s) 299, 480
Tru1I TTAA 2 cut(s) 506, 560
Tru9I TTAA 2 cut(s) 506, 560
TseI GCWGC 2 cut(s) 366, 369
TspDTI ATGAA 2 cut(s) 456, 672
TspGWI ACGGA 2 cut(s) 179, 617
Van91I CCANNNNNTGG 1 cut(s) 243
VpaK11BI GGWCC 4 cut(s) 167, 252, 322, 613
ZrmI AGTACT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.