Rmu_sc0006282.1_g000033

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006282.1
OG0003425
Physical Location & Seq
Forward (+)
134056 .. 135478
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1254 bp
atggcagaaattggagaaagctctggtgcaaagccctctggtgaaatctgggccaagcttgttccttccgactcgagatttccggatgttgagatcagagatcttgttctgactcaagatacccctcagagtcaagatattgaattacgaggaacccccgatcagtcttctgccactatacaaaataagagttcaaatgcaatacttgttgatggagctgtgatccaatccgaagagagtactgttatcagatctggaagtgaaattattacaggtcttgagagagaagggtgtttaagctacagattcaatgtgttgcttggtcctgaaacttgtcagaagatgctgaagattagtgtggatgctgagcatgcaaatgtactctgtccccaatgtcgagcagttgtgcaatttgttggaagaaaccacttcctgcataacattgccgatgaaatactgaatgtggattcttcactgagacgctccaacgaagacactgcagttttagattcctatgcaacagtacgatcaaatctggtaataaagtcgggaaaggggctgcttgggaaaagggttcgttcagcagtgcaagatgaagctgatagtgtagagcttccctgccctcaatgcagtaatcaatttggtgggtttctctgcaaccgaagcactacccatctgcaatgtcaagcatgtggaggaatgatgccttcacgaaccaatacaagtgtacctcaacactgtatagggtgtgataggtcattttgcggtgcatattggcatgctcaaggcattgcacaaagtgcttctcaccacatatgcagtcgcgatactttcaaaccaatcacagaacgcaccatctctagaattcctacttcagcacatgaaaacaatcgccatgaacaagaaatcactatgaggtgtattgcacaaatgggaagaacattgcaggatgttattgccgaatggatggtaatgttcaataacagaacaattgatcgaactaacaggatgccactgaagcatgccgagctgataacttctgggacatatacatgcagtgattgttatgagaaactgatcgcattcctcttgtactggtttcgggtttcagtttctgcatctcatcttcccccagatgcatcaaaaagggaaaattgctggtatggatatgcatgtcgaacacagcaccataatgaagaccatgctcgtaaaaggaatcatgtatgccggccgactaggggagctaatgtgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000209 GO:0000278 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016605 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0036094 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045786 GO:0045862 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051726 GO:0060255 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1903047 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

417

Amino Acids

46.48

Weight (kDa)

6.95

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CRD PF17979 207 - 370 9.3e-48 Cysteine rich domain with multizinc binding regions
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 825
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 1 cut(s) 765
AclWI GGATC 1 cut(s) 217
AcoI YGGCCR 1 cut(s) 1229
AcsI RAATTY 1 cut(s) 864
AcuI CTGAAG 3 cut(s) 368, 858, 1037
AdeI CACNNNGTG 1 cut(s) 800
AfaI GTAC 5 cut(s) 241, 381, 525, 729, 1094
AfiI CCNNNNNNNGG 1 cut(s) 1238
AgsI TTSAA 5 cut(s) 143, 195, 310, 835, 979
AluBI AGCT 8 cut(s) 21, 58, 218, 300, 599, 613, 1030, 1244
AluI AGCT 8 cut(s) 21, 58, 218, 300, 599, 613, 1030, 1244
Alw26I GTCTC 1 cut(s) 472
AlwI GGATC 1 cut(s) 217
AlwNI CAGNNNCTG 1 cut(s) 1115
Ama87I CYCGRG 1 cut(s) 73
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 2 cut(s) 51, 1229
ApeKI GCWGC 1 cut(s) 559
ApoI RAATTY 1 cut(s) 864
AspS9I GGNCC 2 cut(s) 51, 323
AsuHPI GGTGA 2 cut(s) 53, 800
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BbsI GAAGAC 3 cut(s) 159, 498, 1203
BbvI GCAGC 1 cut(s) 546
BccI CCATC 4 cut(s) 206, 681, 863, 961
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BcoDI GTCTC 1 cut(s) 472
BfaI CTAG 2 cut(s) 861, 1236
BfmI CTRYAG 2 cut(s) 301, 498
BglII AGATCT 2 cut(s) 100, 251
BisI GCNGC 1 cut(s) 560
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 1 cut(s) 561
BmcAI AGTACT 1 cut(s) 241
Bme18I GGWCC 1 cut(s) 323
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 2 cut(s) 51, 323
BmiI GGNNCC 1 cut(s) 154
BmsI GCATC 7 cut(s) 333, 352, 693, 999, 1126, 1127, 1148
BpiI GAAGAC 3 cut(s) 159, 498, 1203
Bpu1102I GCTNAGC 1 cut(s) 366
BpuEI CTTGAG 3 cut(s) 99, 299, 768
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 1 cut(s) 1233
Bsc4I CCNNNNNNNGG 1 cut(s) 1238
Bse118I RCCGGY 1 cut(s) 1227
Bse1I ACTGG 1 cut(s) 1100
Bse3DI GCAATG 4 cut(s) 441, 686, 789, 941
BseAI TCCGGA 1 cut(s) 82
BseGI GGATG 5 cut(s) 91, 367, 955, 972, 1014
BseLI CCNNNNNNNGG 1 cut(s) 1238
BseMI GCAATG 4 cut(s) 441, 686, 789, 941
BseMII CTCAG 3 cut(s) 140, 357, 467
BseNI ACTGG 1 cut(s) 1100
BseX3I CGGCCG 1 cut(s) 1229
BseXI GCAGC 1 cut(s) 546
Bsh1236I CGCG 1 cut(s) 825
Bsh1285I CGRYCG 1 cut(s) 1232
BshFI GGCC 2 cut(s) 53, 1231
BsiEI CGRYCG 1 cut(s) 1232
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 2 cut(s) 83, 1228
BslFI GGGAC 2 cut(s) 372, 1057
BslI CCNNNNNNNGG 1 cut(s) 1238
BsmAI GTCTC 1 cut(s) 472
BsmBI CGTCTC 1 cut(s) 472
BsmFI GGGAC 2 cut(s) 372, 1057
BsmI GAATGC 1 cut(s) 1082
BsnI GGCC 2 cut(s) 53, 1231
BsoBI CYCGRG 1 cut(s) 73
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 8 cut(s) 93, 100, 160, 222, 251, 527, 994, 1077
Bsp1720I GCTNAGC 1 cut(s) 366
Bsp68I TCGCGA 1 cut(s) 825
BspACI CCGC 1 cut(s) 765
BspANI GGCC 2 cut(s) 53, 1231
BspCNI CTCAG 3 cut(s) 139, 358, 468
BspEI TCCGGA 1 cut(s) 82
BspFNI CGCG 1 cut(s) 825
BspLI GGNNCC 1 cut(s) 154
BspMAI CTGCAG 1 cut(s) 502
BspPI GGATC 1 cut(s) 217
BsrDI GCAATG 4 cut(s) 441, 686, 789, 941
BsrFI RCCGGY 1 cut(s) 1227
BsrI ACTGG 1 cut(s) 1100
BssAI RCCGGY 1 cut(s) 1227
BssMI GATC 8 cut(s) 93, 100, 160, 222, 251, 527, 994, 1077
Bst4CI ACNGT 3 cut(s) 244, 523, 740
Bst6I CTCTTC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 800
BstC8I GCNNGC 4 cut(s) 372, 780, 1023, 1229
BstDEI CTNAG 3 cut(s) 126, 366, 476
BstF5I GGATG 5 cut(s) 91, 367, 955, 972, 1014
BstFNI CGCG 1 cut(s) 825
BstKTI GATC 8 cut(s) 96, 103, 163, 225, 254, 530, 997, 1080
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 8 cut(s) 93, 100, 160, 222, 251, 527, 994, 1077
BstMCI CGRYCG 1 cut(s) 1232
BstMWI GCNNNNNNNGC 6 cut(s) 371, 627, 663, 800, 1018, 1027
BstNSI RCATGY 6 cut(s) 374, 693, 782, 1025, 1056, 1176
BstSFI CTRYAG 2 cut(s) 301, 498
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 1 cut(s) 546
BstV2I GAAGAC 3 cut(s) 159, 498, 1203
BstX2I RGATCY 2 cut(s) 100, 251
BstYI RGATCY 2 cut(s) 100, 251
BstZI CGGCCG 1 cut(s) 1229
BsuRI GGCC 2 cut(s) 53, 1231
BtsCI GGATG 5 cut(s) 91, 367, 955, 972, 1014
BtsI GCAGTG 3 cut(s) 495, 591, 1063
BtsIMutI CAGTG 6 cut(s) 473, 495, 591, 736, 1013, 1063
BtuMI TCGCGA 1 cut(s) 825
Cac8I GCNNGC 4 cut(s) 372, 780, 1023, 1229
CaiI CAGNNNCTG 1 cut(s) 1115
Cfr10I RCCGGY 1 cut(s) 1227
Cfr13I GGNCC 2 cut(s) 51, 323
CseI GACGC 1 cut(s) 489
Csp6I GTAC 5 cut(s) 240, 380, 524, 728, 1093
CviQI GTAC 5 cut(s) 240, 380, 524, 728, 1093
DdeI CTNAG 3 cut(s) 126, 366, 476
DpnI GATC 8 cut(s) 95, 102, 162, 224, 253, 529, 996, 1079
DpnII GATC 8 cut(s) 93, 100, 160, 222, 251, 527, 994, 1077
DraIII CACNNNGTG 1 cut(s) 800
EaeI YGGCCR 1 cut(s) 1229
EagI CGGCCG 1 cut(s) 1229
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
EclXI CGGCCG 1 cut(s) 1229
Eco47I GGWCC 1 cut(s) 323
Eco52I CGGCCG 1 cut(s) 1229
Eco57I CTGAAG 3 cut(s) 368, 858, 1037
Eco88I CYCGRG 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 864
EcoT22I ATGCAT 2 cut(s) 1141, 1174
Esp3I CGTCTC 1 cut(s) 472
FaqI GGGAC 2 cut(s) 372, 1057
FauNDI CATATG 1 cut(s) 815
Fnu4HI GCNGC 1 cut(s) 560
FokI GGATG 5 cut(s) 98, 374, 962, 979, 1021
Fsp4HI GCNGC 1 cut(s) 560
FspBI CTAG 2 cut(s) 861, 1236
GluI GCNGC 1 cut(s) 560
HaeIII GGCC 2 cut(s) 53, 1231
HapII CCGG 2 cut(s) 83, 1228
HgaI GACGC 1 cut(s) 489
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 7 cut(s) 71, 112, 130, 306, 467, 509, 1216
HpaII CCGG 2 cut(s) 83, 1228
HphI GGTGA 2 cut(s) 53, 800
Hpy166II GTNNAC 1 cut(s) 728
Hpy188I TCNGA 7 cut(s) 70, 98, 111, 129, 232, 251, 339
Hpy8I GTNNAC 1 cut(s) 728
HpyAV CCTTC 3 cut(s) 75, 281, 717
HpyCH4III ACNGT 3 cut(s) 244, 523, 740
HpyF10VI GCNNNNNNNGC 6 cut(s) 371, 627, 663, 800, 1018, 1027
HpyF3I CTNAG 3 cut(s) 126, 366, 476
Kpn2I TCCGGA 1 cut(s) 82
KroI GCCGGC 1 cut(s) 1227
KroNI GCCGGC 1 cut(s) 1229
Kzo9I GATC 8 cut(s) 93, 100, 160, 222, 251, 527, 994, 1077
LmnI GCTCC 3 cut(s) 215, 488, 1241
Lsp1109I GCAGC 1 cut(s) 546
LweI GCATC 7 cut(s) 333, 352, 693, 999, 1126, 1127, 1148
MaeI CTAG 2 cut(s) 861, 1236
MalI GATC 8 cut(s) 95, 102, 162, 224, 253, 529, 996, 1079
MboI GATC 8 cut(s) 93, 100, 160, 222, 251, 527, 994, 1077
MfeI CAATTG 1 cut(s) 990
MflI RGATCY 2 cut(s) 100, 251
MluCI AATT 8 cut(s) 9, 143, 264, 410, 638, 864, 990, 1153
MlyI GAGTC 3 cut(s) 65, 106, 139
MmeI TCCRAC 3 cut(s) 93, 397, 510
MnlI CCTC 8 cut(s) 46, 135, 143, 633, 689, 741, 909, 1097
Mph1103I ATGCAT 2 cut(s) 1141, 1174
MroI TCCGGA 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 1227
MseI TTAA 1 cut(s) 296
MslI CAYNNNNRTG 3 cut(s) 375, 1051, 1191
MspI CCGG 2 cut(s) 83, 1228
MunI CAATTG 1 cut(s) 990
Mva1269I GAATGC 1 cut(s) 1082
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 6 cut(s) 371, 627, 663, 800, 1018, 1027
NaeI GCCGGC 1 cut(s) 1229
NdeI CATATG 1 cut(s) 815
NdeII GATC 8 cut(s) 93, 100, 160, 222, 251, 527, 994, 1077
NgoMIV GCCGGC 1 cut(s) 1227
NlaIV GGNNCC 1 cut(s) 154
NmeAIII GCCGAG 1 cut(s) 1051
NruI TCGCGA 1 cut(s) 825
NsiI ATGCAT 2 cut(s) 1141, 1174
NspI RCATGY 6 cut(s) 374, 693, 782, 1025, 1056, 1176
PaeI GCATGC 3 cut(s) 374, 782, 1025
PaeR7I CTCGAG 1 cut(s) 73
PctI GAATGC 1 cut(s) 1082
PdiI GCCGGC 1 cut(s) 1229
PfeI GAWTC 4 cut(s) 306, 467, 509, 1216
PkrI GCNGC 1 cut(s) 561
PleI GAGTC 3 cut(s) 65, 106, 138
PpsI GAGTC 3 cut(s) 65, 106, 138
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 2 cut(s) 51, 323
PstI CTGCAG 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 1115
PsuI RGATCY 2 cut(s) 100, 251
RruI TCGCGA 1 cut(s) 825
RsaI GTAC 5 cut(s) 241, 381, 525, 729, 1094
RsaNI GTAC 5 cut(s) 240, 380, 524, 728, 1093
RseI CAYNNNNRTG 3 cut(s) 375, 1051, 1191
SaqAI TTAA 1 cut(s) 296
SatI GCNGC 1 cut(s) 560
Sau3AI GATC 8 cut(s) 93, 100, 160, 222, 251, 527, 994, 1077
Sau96I GGNCC 2 cut(s) 51, 323
ScaI AGTACT 1 cut(s) 241
SchI GAGTC 3 cut(s) 65, 106, 139
SfaNI GCATC 7 cut(s) 333, 352, 693, 999, 1126, 1127, 1148
SfcI CTRYAG 2 cut(s) 301, 498
Sfr274I CTCGAG 1 cut(s) 73
SinI GGWCC 1 cut(s) 323
SlaI CTCGAG 1 cut(s) 73
SmiMI CAYNNNNRTG 3 cut(s) 375, 1051, 1191
SmlI CTYRAG 4 cut(s) 73, 114, 278, 783
SmoI CTYRAG 4 cut(s) 73, 114, 278, 783
SphI GCATGC 3 cut(s) 374, 782, 1025
Sse9I AATT 8 cut(s) 9, 143, 264, 410, 638, 864, 990, 1153
SsiI CCGC 1 cut(s) 765
SspMI CTAG 2 cut(s) 861, 1236
TaaI ACNGT 3 cut(s) 244, 523, 740
TaqI TCGA 4 cut(s) 74, 397, 997, 1177
TasI AATT 8 cut(s) 9, 143, 264, 410, 638, 864, 990, 1153
TatI WGTACW 3 cut(s) 239, 379, 1092
TfiI GAWTC 4 cut(s) 306, 467, 509, 1216
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TscAI CASTG 6 cut(s) 480, 502, 591, 743, 1020, 1063
TseI GCWGC 1 cut(s) 559
TspDTI ATGAA 5 cut(s) 465, 609, 897, 912, 1209
TspRI CASTG 6 cut(s) 480, 502, 591, 743, 1020, 1063
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 864
XbaI TCTAGA 1 cut(s) 860
XceI RCATGY 6 cut(s) 374, 693, 782, 1025, 1056, 1176
XhoI CTCGAG 1 cut(s) 73
XspI CTAG 2 cut(s) 861, 1236
ZrmI AGTACT 1 cut(s) 241
Zsp2I ATGCAT 2 cut(s) 1141, 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.