Rmu_ssc0000100.1_g000024

HAUS augmin-like complex subunit 6 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000100.1
OG0002442
Physical Location & Seq
Forward (+)
96841 .. 98014
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ctgaacggcgattccaacacccagcaatggcctcctcccccggccattatcggactcggagcctctaactccactccccgggtcgggttcttccgccactccaaccccaaactcggcgtgcagctcctctacttcatcctctcttccctccgtggcccaattcaatccgccaaggatttcgataaggtctggccaatcttcgattttcgtaaggttgtgcaagggattattagcgagctcgaatcgcaaggggtgcttcccaggagcaattcgagggtttcatcacttgctacatgttgtggaccgaggtttgtagaacttctgtggcaactttctttgcatgctttgcgagaggttcataggcggacatttgcagctgatgtagcttctaaccctcttcccgcatcattgacagatgtggccttttcacatgcagccactttacttcctgttacaaaggtagtgttgaccacgcacgtaaaacttaaagcatgcatgatgtttgctcccaatagcgttttgaagttgtga

Protein Analysis

176

Amino Acids

19.29

Weight (kDa)

9.95

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS6_N PF14661 27 - 141 1.4e-20 HAUS augmin-like complex subunit 6 N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 94, 168, 364, 402
AcoI YGGCCR 2 cut(s) 42, 191
AfiI CCNNNNNNNGG 5 cut(s) 27, 78, 83, 84, 113
AflIII ACRYGT 1 cut(s) 293
AgsI TTSAA 2 cut(s) 164, 523
AjnI CCWGG 1 cut(s) 260
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 124, 238, 377, 386
AluI AGCT 4 cut(s) 124, 238, 377, 386
Alw21I GWGCWC 1 cut(s) 240
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 5 cut(s) 29, 42, 154, 191, 420
ApeKI GCWGC 3 cut(s) 121, 374, 434
AspS9I GGNCC 2 cut(s) 155, 302
AsuC2I CCSGG 3 cut(s) 41, 79, 80
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 193
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 3 cut(s) 133, 386, 446
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 262
BcnI CCSGG 3 cut(s) 41, 79, 80
BisI GCNGC 3 cut(s) 122, 375, 435
BlsI GCNGC 3 cut(s) 123, 376, 436
Bme1390I CCNGG 4 cut(s) 41, 79, 80, 262
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 2 cut(s) 155, 302
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 4 cut(s) 41, 79, 80, 262
BmsI GCATC 1 cut(s) 413
BpuMI CCSGG 3 cut(s) 41, 79, 80
BsaAI YACGTR 1 cut(s) 478
BsaJI CCNNGG 7 cut(s) 39, 77, 78, 151, 171, 260, 305
Bsc4I CCNNNNNNNGG 5 cut(s) 27, 78, 83, 84, 113
Bse3DI GCAATG 1 cut(s) 32
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 7 cut(s) 39, 77, 78, 151, 171, 260, 305
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 5 cut(s) 27, 78, 83, 84, 113
BseMI GCAATG 1 cut(s) 32
BseRI GAGGAG 2 cut(s) 24, 116
BseXI GCAGC 3 cut(s) 133, 386, 446
BseYI CCCAGC 1 cut(s) 21
BsgI GTGCAG 1 cut(s) 140
BshFI GGCC 5 cut(s) 31, 44, 156, 193, 422
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 2 cut(s) 41, 79
BslI CCNNNNNNNGG 5 cut(s) 27, 78, 83, 84, 113
BsnI GGCC 5 cut(s) 31, 44, 156, 193, 422
BsoBI CYCGRG 1 cut(s) 78
Bsp1286I GDGCHC 1 cut(s) 240
BspACI CCGC 4 cut(s) 94, 168, 364, 402
BspANI GGCC 5 cut(s) 31, 44, 156, 193, 422
BspLI GGNNCC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 32
BssECI CCNNGG 7 cut(s) 39, 77, 78, 151, 171, 260, 305
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 262
Bst6I CTCTTC 2 cut(s) 148, 402
BstAPI GCANNNNNTGC 2 cut(s) 253, 346
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 4 cut(s) 119, 236, 342, 493
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 1 cut(s) 135
BstMWI GCNNNNNNNGC 4 cut(s) 244, 253, 346, 383
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 4 cut(s) 297, 344, 434, 495
BstSCI CCNGG 4 cut(s) 39, 77, 78, 260
BstV1I GCAGC 3 cut(s) 133, 386, 446
BsuRI GGCC 5 cut(s) 31, 44, 156, 193, 422
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 135
Cac8I GCNNGC 4 cut(s) 119, 236, 342, 493
Cfr13I GGNCC 2 cut(s) 155, 302
Cfr9I CCCGGG 1 cut(s) 78
CviAII CATG 5 cut(s) 294, 341, 431, 492, 496
EaeI YGGCCR 2 cut(s) 42, 191
Eam1104I CTCTTC 2 cut(s) 148, 402
EarI CTCTTC 2 cut(s) 148, 402
EciI GGCGGA 3 cut(s) 83, 157, 379
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 302
Eco53kI GAGCTC 1 cut(s) 238
Eco88I CYCGRG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 171
EcoT22I ATGCAT 1 cut(s) 497
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 5 cut(s) 297, 344, 434, 495, 499
FaiI YATR 6 cut(s) 295, 342, 360, 432, 493, 497
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 5 cut(s) 293, 340, 430, 491, 495
FauI CCCGC 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 122, 375, 435
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 3 cut(s) 122, 375, 435
GluI GCNGC 3 cut(s) 122, 375, 435
GsaI CCCAGC 1 cut(s) 25
HaeIII GGCC 5 cut(s) 31, 44, 156, 193, 422
HapII CCGG 2 cut(s) 41, 79
Hin1II CATG 5 cut(s) 297, 344, 434, 495, 499
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HinfI GANTC 3 cut(s) 11, 54, 242
HpaII CCGG 2 cut(s) 41, 79
Hpy166II GTNNAC 2 cut(s) 302, 468
Hpy188I TCNGA 2 cut(s) 53, 59
Hpy8I GTNNAC 2 cut(s) 302, 468
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 6 cut(s) 121, 220, 340, 374, 434, 495
HpyF10VI GCNNNNNNNGC 4 cut(s) 244, 253, 346, 383
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 5 cut(s) 297, 344, 434, 495, 499
LmnI GCTCC 4 cut(s) 59, 129, 264, 511
LpnPI CCDG 7 cut(s) 35, 54, 92, 175, 247, 274, 462
Lsp1109I GCAGC 3 cut(s) 133, 386, 446
LweI GCATC 1 cut(s) 413
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 1 cut(s) 451
MboII GAAGA 4 cut(s) 82, 135, 190, 389
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 193
MluCI AATT 2 cut(s) 159, 268
MluNI TGGCCA 1 cut(s) 193
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 2 cut(s) 39, 126
Mox20I TGGCCA 1 cut(s) 193
Mph1103I ATGCAT 1 cut(s) 497
MscI TGGCCA 1 cut(s) 193
MseI TTAA 1 cut(s) 486
Msp20I TGGCCA 1 cut(s) 193
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 2 cut(s) 41, 79
MspR9I CCNGG 4 cut(s) 41, 79, 80, 262
MvaI CCWGG 1 cut(s) 262
MwoI GCNNNNNNNGC 4 cut(s) 244, 253, 346, 383
NciI CCSGG 3 cut(s) 41, 79, 80
NlaIII CATG 5 cut(s) 297, 344, 434, 495, 499
NlaIV GGNNCC 1 cut(s) 61
NmeAIII GCCGAG 1 cut(s) 93
NsiI ATGCAT 1 cut(s) 497
NspI RCATGY 4 cut(s) 297, 344, 434, 495
PaeI GCATGC 2 cut(s) 344, 495
PciI ACATGT 1 cut(s) 293
PfeI GAWTC 2 cut(s) 11, 242
PkrI GCNGC 3 cut(s) 123, 376, 436
PleI GAGTC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 48
Ppu21I YACGTR 1 cut(s) 478
PscI ACATGT 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 21
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 2 cut(s) 155, 302
PvuII CAGCTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 3 cut(s) 122, 375, 435
Sau96I GGNCC 2 cut(s) 155, 302
SchI GAGTC 1 cut(s) 48
ScrFI CCNGG 4 cut(s) 41, 79, 80, 262
SduI GDGCHC 1 cut(s) 240
SfaNI GCATC 1 cut(s) 413
SinI GGWCC 1 cut(s) 302
SmaI CCCGGG 1 cut(s) 80
SphI GCATGC 2 cut(s) 344, 495
Sse9I AATT 2 cut(s) 159, 268
SsiI CCGC 4 cut(s) 94, 168, 364, 402
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 4 cut(s) 39, 77, 78, 260
StyI CCWWGG 1 cut(s) 171
TaiI ACGT 1 cut(s) 480
TaqI TCGA 4 cut(s) 180, 201, 240, 272
TaqII GACCGA 1 cut(s) 319
TasI AATT 2 cut(s) 159, 268
TfiI GAWTC 2 cut(s) 11, 242
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TseI GCWGC 3 cut(s) 121, 374, 434
TspDTI ATGAA 3 cut(s) 124, 270, 347
TspGWI ACGGA 1 cut(s) 140
TspMI CCCGGG 1 cut(s) 78
VpaK11BI GGWCC 1 cut(s) 302
XceI RCATGY 4 cut(s) 297, 344, 434, 495
XmaI CCCGGG 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.