Rmu_ssc0000124.1_g000015

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000124.1
OG0007094
Physical Location & Seq
Reverse (-)
62889 .. 66360
3472 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1725 bp
atgttaactgcattggaggatgagtcagaaactgctgttgatgaaccctcgagtgtggtgaagcctagtgctgatgtggtttcgacatccagtcaaaaatccaagaagaagaagaagaagaaaggcaaagagggcccttcttctaatgcagctgcaccgagagttaaaaagtcgccggatgacgttctggaagaattctatgagtatgcgcatgaaagacaagccgagacgagggctagtggtgatgcggttaagcattcgtatgaaaagtatgtgcccttcatattgcaagtggatcccaagtacttgaatgctgacaatgagatgcgaagaatctttggggctaaggtggtcaagtcgtttgagaagaatcaacagagtggtagctctagaatggcgcgtggtggaagacgcggcgcggttcataggaagtccttccttgtcactccgttggagcattggcctcgctgggacggtgctatgaacatggaactccttcactcccccaatggatactatgattaccgatatacctattcatcagcctaccatcaagctcagagtgcatttgaatctgccaaagctatacatgatctcaatgccattgcaagtgttttattatactatccttaccatttggattcgctactaaccatggcagagtactttaagtttgtgggtgagcatcaaatgtcagcagatgctatctcgaagtgcttatatgctttggaatgtgcatggtctccaatgttcaccccctttaagggtgattgccaagtgaaatacagccatgaagcaaacaaacctctgttcgttgcactttttactcacatgaaaagtatggacagacgtggctgtcatcgatctgctttagaagtttgcaagtttttactgtcactagatactgacgatccaatgggggccatgttctgtattgactattttgcggtgagggcagaggagtatgcatggctagaacgtttctctgaagaatataaaagtgataacaccctatggttgtttccaaatttttcatattcccttgccgtatgccgattgtatcttgagaaagcagaatcttccaaagatccagctatggaaaccaccaaggctacttcaactgatcttatgaagcaggcattgatgcttcatcccacagtcctcaagaaactagtggcaaaagtacctttgaaggaacgggtgtggacggatatactcaaaaatgcattcttccaagcagatgaagtaggaatcccatccttggaccacctgattaacatatacgttgagagtaatcatattatatggaggcttccagaattgcagaaattgctgcgggaagcagcacagctggtgatcgagactctaagacagaatagtagcgaaaagaatgattgggcatgtgtgagaaaagaagcattctcatctgacaagaatgagtattcccacttgttggtttcagacttctcttatacattgcctgctgccccacctgaaaatttgcaacattttatggccaatgcaaggatggaggatgttgtgcataatgagcagcatcttgtcaatctaaatgagggtggccatgctccccgtgatgttgcaaatcgaaatccattagcggtcttgtttgagtcggtgttaccatgggttcattatggggaaggagcgtttgttgaggaggaagaccaacaaaatggccatggtccgggcaatcaagaacattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tcf25

Protein Analysis

574

Amino Acids

65.43

Weight (kDa)

6.05

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tcf25 PF04910 175 - 499 9.1e-69 Transcriptional repressor TCF25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 855
Acc16I TGCGCA 1 cut(s) 210
AccB7I CCANNNNNTGG 1 cut(s) 1453
AccII CGCG 3 cut(s) 400, 414, 419
AciI CCGC 6 cut(s) 248, 414, 419, 947, 1336, 1619
AclI AACGTT 1 cut(s) 979
AclWI GGATC 4 cut(s) 290, 303, 905, 1082
AcoI YGGCCR 3 cut(s) 1515, 1579, 1696
AcsI RAATTY 3 cut(s) 194, 1027, 1498
AcuI CTGAAG 1 cut(s) 1008
AfaI GTAC 3 cut(s) 305, 665, 1185
AfiI CCNNNNNNNGG 7 cut(s) 231, 763, 764, 1261, 1453, 1524, 1705
AgsI TTSAA 4 cut(s) 310, 572, 1119, 1192
AhdI GACNNNNNGTC 1 cut(s) 90
AhlI ACTAGT 1 cut(s) 1171
AjiI CACGTC 1 cut(s) 851
AluBI AGCT 6 cut(s) 152, 387, 557, 584, 1094, 1351
AluI AGCT 6 cut(s) 152, 387, 557, 584, 1094, 1351
Alw26I GTCTC 3 cut(s) 221, 747, 1355
AlwI GGATC 4 cut(s) 290, 303, 905, 1082
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 6 cut(s) 133, 461, 921, 1515, 1579, 1696
ApaI GGGCCC 1 cut(s) 137
ApeKI GCWGC 6 cut(s) 149, 152, 1333, 1343, 1484, 1552
ApoI RAATTY 3 cut(s) 194, 1027, 1498
Asp700I GAANNNNTTC 2 cut(s) 434, 495
AspLEI GCGC 3 cut(s) 211, 400, 419
AspS9I GGNCC 5 cut(s) 133, 134, 921, 1264, 1703
AsuC2I CCSGG 1 cut(s) 1707
AsuHPI GGTGA 7 cut(s) 70, 254, 692, 745, 779, 961, 1366
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 2 cut(s) 1264, 1703
BaeGI GKGCMC 2 cut(s) 137, 279
BalI TGGCCA 3 cut(s) 1517, 1581, 1698
BamHI GGATCC 1 cut(s) 295
BanII GRGCYC 1 cut(s) 137
BarI GAAGNNNNNNTAC 2 cut(s) 263, 295
BbsI GAAGAC 2 cut(s) 415, 1689
BbvI GCAGC 6 cut(s) 139, 161, 1320, 1355, 1471, 1564
BccI CCATC 3 cut(s) 558, 1264, 1522
BceAI ACGGC 1 cut(s) 1031
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciVI GTATCC 1 cut(s) 506
BcnI CCSGG 1 cut(s) 1707
BcoDI GTCTC 3 cut(s) 221, 747, 1355
BcuI ACTAGT 1 cut(s) 1171
BfaI CTAG 6 cut(s) 66, 237, 390, 899, 974, 1172
BfuI GTATCC 1 cut(s) 506
BisI GCNGC 7 cut(s) 150, 153, 415, 1334, 1344, 1485, 1553
BlsI GCNGC 7 cut(s) 151, 154, 416, 1335, 1345, 1486, 1554
BmcAI AGTACT 2 cut(s) 305, 665
Bme1390I CCNGG 1 cut(s) 1707
Bme18I GGWCC 2 cut(s) 1264, 1703
BmeRI GACNNNNNGTC 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 49
BmgBI CACGTC 1 cut(s) 851
BmgT120I GGNCC 5 cut(s) 133, 134, 921, 1264, 1703
BmiI GGNNCC 3 cut(s) 135, 297, 922
BmrFI CCNGG 1 cut(s) 1707
BmsI GCATC 6 cut(s) 235, 315, 691, 694, 1134, 1564
BpiI GAAGAC 2 cut(s) 415, 1689
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 2 cut(s) 1085, 1148
BpuMI CCSGG 1 cut(s) 1707
Bsa29I ATCGAT 1 cut(s) 862
BsaI GGTCTC 1 cut(s) 747
BsaJI CCNNGG 5 cut(s) 654, 1107, 1260, 1643, 1699
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 1656, 1686
Bsc4I CCNNNNNNNGG 7 cut(s) 231, 763, 764, 1261, 1453, 1524, 1705
Bse1I ACTGG 1 cut(s) 90
Bse3DI GCAATG 2 cut(s) 603, 1475
BseCI ATCGAT 1 cut(s) 862
BseDI CCNNGG 5 cut(s) 654, 1107, 1260, 1643, 1699
BseGI GGATG 7 cut(s) 25, 86, 184, 1150, 1256, 1533, 1540
BseLI CCNNNNNNNGG 7 cut(s) 231, 763, 764, 1261, 1453, 1524, 1705
BseMI GCAATG 2 cut(s) 603, 1475
BseMII CTCAG 1 cut(s) 572
BseNI ACTGG 1 cut(s) 90
BseRI GAGGAG 2 cut(s) 974, 1691
BseSI GKGCMC 2 cut(s) 137, 279
BseXI GCAGC 6 cut(s) 139, 161, 1320, 1355, 1471, 1564
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 1 cut(s) 138
Bsh1236I CGCG 3 cut(s) 400, 414, 419
BshFI GGCC 6 cut(s) 135, 463, 923, 1517, 1581, 1698
BshVI ATCGAT 1 cut(s) 862
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 2 cut(s) 176, 1706
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 7 cut(s) 231, 763, 764, 1261, 1453, 1524, 1705
BsmAI GTCTC 3 cut(s) 221, 747, 1355
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 485
BsmI GAATGC 4 cut(s) 256, 316, 1226, 1418
BsnI GGCC 6 cut(s) 135, 463, 923, 1517, 1581, 1698
Bso31I GGTCTC 1 cut(s) 747
BsoBI CYCGRG 1 cut(s) 49
Bsp120I GGGCCC 1 cut(s) 133
Bsp1286I GDGCHC 2 cut(s) 137, 279
Bsp143I GATC 7 cut(s) 295, 592, 863, 910, 1087, 1123, 1356
Bsp19I CCATGG 3 cut(s) 654, 1643, 1699
BspACI CCGC 6 cut(s) 248, 414, 419, 947, 1336, 1619
BspANI GGCC 6 cut(s) 135, 463, 923, 1517, 1581, 1698
BspCNI CTCAG 1 cut(s) 571
BspDI ATCGAT 1 cut(s) 862
BspFNI CGCG 3 cut(s) 400, 414, 419
BspLI GGNNCC 3 cut(s) 135, 297, 922
BspPI GGATC 4 cut(s) 290, 303, 905, 1082
BspTNI GGTCTC 1 cut(s) 747
BsrDI GCAATG 2 cut(s) 603, 1475
BsrI ACTGG 1 cut(s) 90
BssECI CCNNGG 5 cut(s) 654, 1107, 1260, 1643, 1699
BssMI GATC 7 cut(s) 295, 592, 863, 910, 1087, 1123, 1356
BssT1I CCWWGG 5 cut(s) 654, 1107, 1260, 1643, 1699
Bst4CI ACNGT 3 cut(s) 476, 894, 1159
BstAPI GCANNNNNTGC 1 cut(s) 1330
BstC8I GCNNGC 2 cut(s) 1137, 1482
BstDEI CTNAG 3 cut(s) 345, 558, 1367
BstDSI CCRYGG 3 cut(s) 654, 1643, 1699
BstF5I GGATG 7 cut(s) 25, 86, 184, 1150, 1256, 1533, 1540
BstFNI CGCG 3 cut(s) 400, 414, 419
BstHHI GCGC 3 cut(s) 211, 400, 419
BstKTI GATC 7 cut(s) 298, 595, 866, 913, 1090, 1126, 1359
BstMAI GTCTC 3 cut(s) 221, 747, 1355
BstMBI GATC 7 cut(s) 295, 592, 863, 910, 1087, 1123, 1356
BstMWI GCNNNNNNNGC 5 cut(s) 132, 563, 953, 1330, 1549
BstNSI RCATGY 1 cut(s) 1404
BstSCI CCNGG 1 cut(s) 1705
BstSLI GKGCMC 2 cut(s) 137, 279
BstUI CGCG 3 cut(s) 400, 414, 419
BstV1I GCAGC 6 cut(s) 139, 161, 1320, 1355, 1471, 1564
BstV2I GAAGAC 2 cut(s) 415, 1689
BstX2I RGATCY 2 cut(s) 295, 1087
BstXI CCANNNNNNTGG 1 cut(s) 1694
BstYI RGATCY 2 cut(s) 295, 1087
Bsu15I ATCGAT 1 cut(s) 862
BsuI GTATCC 1 cut(s) 506
BsuRI GGCC 6 cut(s) 135, 463, 923, 1517, 1581, 1698
BsuTUI ATCGAT 1 cut(s) 862
BtgI CCRYGG 3 cut(s) 654, 1643, 1699
BtrI CACGTC 1 cut(s) 851
BtsCI GGATG 7 cut(s) 25, 86, 184, 1150, 1256, 1533, 1540
Cac8I GCNNGC 2 cut(s) 1137, 1482
CaiI CAGNNNCTG 1 cut(s) 32
CfoI GCGC 3 cut(s) 211, 400, 419
Cfr13I GGNCC 5 cut(s) 133, 134, 921, 1264, 1703
ClaI ATCGAT 1 cut(s) 862
CseI GACGC 1 cut(s) 420
Csp6I GTAC 3 cut(s) 304, 664, 1184
CviQI GTAC 3 cut(s) 304, 664, 1184
DdeI CTNAG 3 cut(s) 345, 558, 1367
DpnI GATC 7 cut(s) 297, 594, 865, 912, 1089, 1125, 1358
DpnII GATC 7 cut(s) 295, 592, 863, 910, 1087, 1123, 1356
DrdI GACNNNNNNGTC 1 cut(s) 855
DriI GACNNNNNGTC 1 cut(s) 90
DseDI GACNNNNNNGTC 1 cut(s) 855
EaeI YGGCCR 3 cut(s) 1515, 1579, 1696
Eam1105I GACNNNNNGTC 1 cut(s) 90
Eco130I CCWWGG 5 cut(s) 654, 1107, 1260, 1643, 1699
Eco24I GRGCYC 1 cut(s) 137
Eco31I GGTCTC 1 cut(s) 747
Eco47I GGWCC 2 cut(s) 1264, 1703
Eco57I CTGAAG 1 cut(s) 1008
Eco88I CYCGRG 1 cut(s) 49
EcoO109I RGGNCCY 2 cut(s) 133, 134
EcoRI GAATTC 1 cut(s) 194
EcoT14I CCWWGG 5 cut(s) 654, 1107, 1260, 1643, 1699
EcoT22I ATGCAT 2 cut(s) 970, 1228
EcoT38I GRGCYC 1 cut(s) 137
ErhI CCWWGG 5 cut(s) 654, 1107, 1260, 1643, 1699
Esp3I CGTCTC 1 cut(s) 221
FaqI GGGAC 1 cut(s) 485
FauI CCCGC 1 cut(s) 1329
Fnu4HI GCNGC 7 cut(s) 150, 153, 415, 1334, 1344, 1485, 1553
FokI GGATG 7 cut(s) 32, 73, 191, 1137, 1243, 1540, 1547
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 7 cut(s) 150, 153, 415, 1334, 1344, 1485, 1553
FspAI RTGCGCAY 1 cut(s) 210
FspBI CTAG 6 cut(s) 66, 237, 390, 899, 974, 1172
FspI TGCGCA 1 cut(s) 210
GlaI GCGC 3 cut(s) 210, 399, 418
GluI GCNGC 7 cut(s) 150, 153, 415, 1334, 1344, 1485, 1553
GsaI CCCAGC 1 cut(s) 472
HaeIII GGCC 6 cut(s) 135, 463, 923, 1517, 1581, 1698
HapII CCGG 2 cut(s) 176, 1706
HgaI GACGC 1 cut(s) 420
HhaI GCGC 3 cut(s) 211, 400, 419
Hin6I GCGC 3 cut(s) 209, 398, 417
HinP1I GCGC 3 cut(s) 209, 398, 417
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 9 cut(s) 23, 333, 370, 572, 641, 1076, 1251, 1363, 1631
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 2 cut(s) 176, 1706
HphI GGTGA 7 cut(s) 70, 254, 692, 745, 779, 961, 1366
Hpy166II GTNNAC 3 cut(s) 6, 753, 1206
Hpy188I TCNGA 5 cut(s) 28, 561, 988, 1429, 1462
Hpy188III TCNNGA 8 cut(s) 188, 390, 709, 1064, 1165, 1316, 1360, 1715
Hpy8I GTNNAC 3 cut(s) 6, 753, 1206
HpyAV CCTTC 6 cut(s) 147, 289, 445, 506, 1186, 1655
HpyCH4III ACNGT 3 cut(s) 476, 894, 1159
HpyCH4IV ACGT 4 cut(s) 183, 850, 979, 1284
HpyF10VI GCNNNNNNNGC 5 cut(s) 132, 563, 953, 1330, 1549
HpyF3I CTNAG 3 cut(s) 345, 558, 1367
HpySE526I ACGT 4 cut(s) 183, 850, 979, 1284
HspAI GCGC 3 cut(s) 209, 398, 417
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 7 cut(s) 295, 592, 863, 910, 1087, 1123, 1356
LmnI GCTCC 3 cut(s) 454, 1591, 1664
Lsp1109I GCAGC 6 cut(s) 139, 161, 1320, 1355, 1471, 1564
LweI GCATC 6 cut(s) 235, 315, 691, 694, 1134, 1564
MaeI CTAG 6 cut(s) 66, 237, 390, 899, 974, 1172
MaeII ACGT 4 cut(s) 183, 850, 979, 1284
MaeIII GTNAC 3 cut(s) 442, 894, 1638
MalI GATC 7 cut(s) 297, 594, 865, 912, 1089, 1125, 1358
MboI GATC 7 cut(s) 295, 592, 863, 910, 1087, 1123, 1356
MflI RGATCY 2 cut(s) 295, 1087
MhlI GDGCHC 2 cut(s) 137, 279
MlsI TGGCCA 3 cut(s) 1517, 1581, 1698
MluCI AATT 5 cut(s) 194, 1027, 1319, 1328, 1498
MluNI TGGCCA 3 cut(s) 1517, 1581, 1698
MlyI GAGTC 3 cut(s) 32, 1357, 1640
MmeI TCCRAC 1 cut(s) 432
Mox20I TGGCCA 3 cut(s) 1517, 1581, 1698
Mph1103I ATGCAT 2 cut(s) 970, 1228
MroXI GAANNNNTTC 2 cut(s) 434, 495
MscI TGGCCA 3 cut(s) 1517, 1581, 1698
MseI TTAA 6 cut(s) 5, 165, 252, 669, 762, 1275
MslI CAYNNNNRTG 1 cut(s) 261
Msp20I TGGCCA 3 cut(s) 1517, 1581, 1698
MspA1I CMGCKG 2 cut(s) 152, 1351
MspI CCGG 2 cut(s) 176, 1706
MspR9I CCNGG 1 cut(s) 1707
Mva1269I GAATGC 4 cut(s) 256, 316, 1226, 1418
MvnI CGCG 3 cut(s) 400, 414, 419
MwoI GCNNNNNNNGC 5 cut(s) 132, 563, 953, 1330, 1549
NciI CCSGG 1 cut(s) 1707
NcoI CCATGG 3 cut(s) 654, 1643, 1699
NdeII GATC 7 cut(s) 295, 592, 863, 910, 1087, 1123, 1356
NlaIV GGNNCC 3 cut(s) 135, 297, 922
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 2 cut(s) 442, 894
NsbI TGCGCA 1 cut(s) 210
NsiI ATGCAT 2 cut(s) 970, 1228
NspI RCATGY 1 cut(s) 1404
PaeR7I CTCGAG 1 cut(s) 49
PctI GAATGC 4 cut(s) 256, 316, 1226, 1418
PdmI GAANNNNTTC 2 cut(s) 434, 495
PfeI GAWTC 6 cut(s) 333, 370, 572, 641, 1076, 1251
PflMI CCANNNNNTGG 1 cut(s) 1453
PkrI GCNGC 7 cut(s) 151, 154, 416, 1335, 1345, 1486, 1554
PleI GAGTC 3 cut(s) 31, 1357, 1639
PpsI GAGTC 3 cut(s) 31, 1357, 1639
Psp1406I AACGTT 1 cut(s) 979
PspFI CCCAGC 1 cut(s) 468
PspN4I GGNNCC 3 cut(s) 135, 297, 922
PspOMI GGGCCC 1 cut(s) 133
PspPI GGNCC 5 cut(s) 133, 134, 921, 1264, 1703
PspXI VCTCGAGB 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 2 cut(s) 295, 1087
PvuII CAGCTG 2 cut(s) 152, 1351
RsaI GTAC 3 cut(s) 305, 665, 1185
RsaNI GTAC 3 cut(s) 304, 664, 1184
RseI CAYNNNNRTG 1 cut(s) 261
SaqAI TTAA 6 cut(s) 5, 165, 252, 669, 762, 1275
SatI GCNGC 7 cut(s) 150, 153, 415, 1334, 1344, 1485, 1553
Sau3AI GATC 7 cut(s) 295, 592, 863, 910, 1087, 1123, 1356
Sau96I GGNCC 5 cut(s) 133, 134, 921, 1264, 1703
ScaI AGTACT 2 cut(s) 305, 665
SchI GAGTC 3 cut(s) 32, 1357, 1640
ScrFI CCNGG 1 cut(s) 1707
SduI GDGCHC 2 cut(s) 137, 279
SfaNI GCATC 6 cut(s) 235, 315, 691, 694, 1134, 1564
Sfr274I CTCGAG 1 cut(s) 49
SinI GGWCC 2 cut(s) 1264, 1703
SlaI CTCGAG 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 3 cut(s) 49, 1064, 1163
SmoI CTYRAG 3 cut(s) 49, 1064, 1163
SpeI ACTAGT 1 cut(s) 1171
Sse9I AATT 5 cut(s) 194, 1027, 1319, 1328, 1498
SsiI CCGC 6 cut(s) 248, 414, 419, 947, 1336, 1619
SspMI CTAG 6 cut(s) 66, 237, 390, 899, 974, 1172
StyD4I CCNGG 1 cut(s) 1705
StyI CCWWGG 5 cut(s) 654, 1107, 1260, 1643, 1699
TaaI ACNGT 3 cut(s) 476, 894, 1159
TaiI ACGT 4 cut(s) 186, 853, 982, 1287
TaqI TCGA 6 cut(s) 50, 83, 710, 862, 1359, 1606
TasI AATT 5 cut(s) 194, 1027, 1319, 1328, 1498
TatI WGTACW 2 cut(s) 303, 663
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 6 cut(s) 333, 370, 572, 641, 1076, 1251
Tru1I TTAA 6 cut(s) 5, 165, 252, 669, 762, 1275
Tru9I TTAA 6 cut(s) 5, 165, 252, 669, 762, 1275
TseFI GTSAC 2 cut(s) 442, 894
TseI GCWGC 6 cut(s) 149, 152, 1333, 1343, 1484, 1552
Tsp45I GTSAC 2 cut(s) 442, 894
TspGWI ACGGA 2 cut(s) 438, 1223
Van91I CCANNNNNTGG 1 cut(s) 1453
VpaK11BI GGWCC 2 cut(s) 1264, 1703
XapI RAATTY 3 cut(s) 194, 1027, 1498
XbaI TCTAGA 1 cut(s) 389
XceI RCATGY 1 cut(s) 1404
XcmI CCANNNNNNNNNTGG 1 cut(s) 1525
XhoI CTCGAG 1 cut(s) 49
XmnI GAANNNNTTC 2 cut(s) 434, 495
XspI CTAG 6 cut(s) 66, 237, 390, 899, 974, 1172
ZrmI AGTACT 2 cut(s) 305, 665
Zsp2I ATGCAT 2 cut(s) 970, 1228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.