Rmu_ssc0000135.1_g000034

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000135.1
OG0002962
Physical Location & Seq
Forward (+)
168565 .. 171772
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2256 bp
atggcctgcacttcattatctatacctagctctccggcttcaacgaggactatagacgtcttcaaggagcagagtggtcttcggcgatcgcaatccagcaaagacctttacagacgtgcaaatattcggaggtcttattccgataaccatctttgctaccgcattaggcctattcatgctgcgtcagcccaaccaaaactaaagaacagccggtccatggggtttttcccctttcaattttcgagctccattctaccaaactcggttagatcatttttgtttgatgcagacacgagtaagaatatgagcacggcagacagggaaatggaaatgatcgataactctagggagagcggtgaagaagaagaagatcatgagataaagagagccaattgggtggagaggcttgtggagatcagaagaaaatggagtaataggcagcaaaaagaagttggggatgaagacagtgtttgtcacgaaggtgatggtgattgtgaatgtggagagggtggttgtttagtggattacagtacagatgaagatgatggagagataacttcagaaagtttccaaaggttattggtcaaggtaccatggtctgataccaaagggtttccaagatcaaaagaaggtgggtgtggctcctttgagtctcacttaccctactactactgctgttcaagagggcaaccctatagatacgggcaacggaaagctctacgatttctgtttcttgatgaagatgatggagagataacttcagaaagtttccaaaggttattggtcaaggtaccatggtctgataccaaacaattttctcagctagccttcttgagcaacctggcatatgtaataccattgataaaggccgatgatttgaagagatattacggcctacagtttgtaacatcttcattaataaagaaagcagaggaagaggaactcaaagaagaaattgatcaggactctactcgtgtacctgtaactgacgaggaatccacttcagaggcagctgcagactctgagcagaagcattcaatccatccatctgttgcttacgaaattgctgcctcagcagcatcttatgttcgctcgcgtgacatggatggtggtgatgtagagaattcattcagaagtggagaccaacaacaacaacgagaggaaggagggagctccccccgaaattataagtcggaggtagctgtttccttaacagcatcaacgatgacagtcgtggttgcagcaggggaaaaacaaaagcaagaaacagcacaagaccttcggtcaccccaatctgcaccttgtgaatggtttgtttgtgatgacaacagtacatatactcgctgctttgtgattcagggttcggactcccttgcgtcctggcaggcaaacctcttctttgaacctacaaaatttgagggaacaaatgtacttgttcacagaggaatctatgaagcagcaaaggggatgtacgagcaattcatgccacagataatcgagcacttggatagatatggagagcgtgcaaagttacagttcactggtcattctcttggaggaagcctctctcttctagtccacttgatgttactgaccaggaactttgtcaagccctccactcttcggccagttgtgacatttggctcaccatttgtgttttgtggaggccagaaaatactagacgacctgggtgtcgatgagaaccaaatccattgcgtgatgatgcatagagatattgtcccaagagccttctcctgcaactaccctaaccatgtggctacgcttctcaagcgtttaaatggttcatttcgatcacacccttgtctgataaaaaacaaaatattgtactctcctatgggtaaaatattcattctgcaacctgatgaaagcttgtctccgccacaccctcttctccccttggggagtgccctctatactttggacaaaactcaacaagggtactcttcatgcgtccttagaaccttcttaaattccccgcatccactagaaaccctaagcgatcctacagcttatggttcggaaggtacaatcctacgggatcatgactcgagcaactatctgaaggctgtgaatggtgtccttaggctacacacaagaaaggttgttcgtatatctaggaaacagaggaatgtcttgtggccgctacttacttcaccatccccacacacatggaaccatgaaaataatctggagagcggcagattggtgacgaaggagattctcacaggagtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

751

Amino Acids

84.73

Weight (kDa)

6.01

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 450 - 587 1.9e-25 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1188
AasI GACNNNNNNGTC 1 cut(s) 1691
AatII GACGTC 1 cut(s) 60
Acc65I GGTACC 2 cut(s) 589, 790
AccB1I GGYRCC 2 cut(s) 589, 790
AccBSI CCGCTC 2 cut(s) 354, 2217
AccII CGCG 1 cut(s) 1096
AciI CCGC 6 cut(s) 160, 354, 1898, 1997, 2162, 2217
AclWI GGATC 2 cut(s) 2015, 2067
AcoI YGGCCR 2 cut(s) 1625, 2159
AcsI RAATTY 3 cut(s) 1123, 1412, 1990
AcuI CTGAAG 4 cut(s) 543, 744, 987, 2102
AcyI GRCGYC 1 cut(s) 57
AdeI CACNNNGTG 1 cut(s) 1304
AfiI CCNNNNNNNGG 2 cut(s) 217, 1623
AgsI TTSAA 7 cut(s) 42, 64, 236, 681, 880, 1038, 1403
AhdI GACNNNNNGTC 1 cut(s) 1282
AjiI CACGTC 1 cut(s) 116
AjnI CCWGG 4 cut(s) 840, 1379, 1595, 1686
AleI CACNNNNGTG 1 cut(s) 480
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 9 cut(s) 30, 246, 716, 823, 1013, 1173, 1202, 1890, 2030
AluI AGCT 9 cut(s) 30, 246, 716, 823, 1013, 1173, 1202, 1890, 2030
Alw21I GWGCWC 4 cut(s) 248, 311, 1175, 1503
Alw26I GTCTC 3 cut(s) 657, 1134, 1899
AlwI GGATC 2 cut(s) 2015, 2067
AlwNI CAGNNNCTG 1 cut(s) 1022
Ama87I CYCGRG 1 cut(s) 2068
AoxI GGCC 7 cut(s) 3, 167, 867, 892, 1625, 1666, 2159
ApeKI GCWGC 9 cut(s) 179, 439, 1010, 1013, 1067, 1076, 1241, 1344, 1457
ApoI RAATTY 3 cut(s) 1123, 1412, 1990
AseI ATTAAT 1 cut(s) 917
AsiSI GCGATCGC 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 1127
Asp718I GGTACC 2 cut(s) 589, 790
AspS9I GGNCC 1 cut(s) 213
AsuHPI GGTGA 8 cut(s) 368, 494, 500, 1124, 1278, 1638, 2166, 2239
AsuNHI GCTAGC 1 cut(s) 823
AvaI CYCGRG 1 cut(s) 2068
AvaII GGWCC 1 cut(s) 213
AxyI CCTNAGG 1 cut(s) 2102
BaeGI GKGCMC 1 cut(s) 1930
BaeI ACNNNNGTAYC 6 cut(s) 581, 614, 782, 815, 960, 993
BanI GGYRCC 2 cut(s) 589, 790
BanII GRGCYC 2 cut(s) 248, 1175
BarI GAAGNNNNNNTAC 2 cut(s) 872, 904
BauI CACGAG 2 cut(s) 292, 972
BbsI GAAGAC 3 cut(s) 52, 71, 468
Bbv12I GWGCWC 4 cut(s) 248, 311, 1175, 1503
BbvCI CCTCAGC 1 cut(s) 1072
BbvI GCAGC 9 cut(s) 166, 451, 1000, 1022, 1054, 1088, 1253, 1331, 1469
BccI CCATC 8 cut(s) 156, 479, 539, 740, 1050, 1054, 1100, 2185
BceAI ACGGC 2 cut(s) 327, 907
BcgI CGANNNNNNTGC 2 cut(s) 1049, 1083
BciT130I CCWGG 4 cut(s) 842, 1381, 1597, 1688
BclI TGATCA 1 cut(s) 958
BcoDI GTCTC 3 cut(s) 657, 1134, 1899
BfaI CTAG 7 cut(s) 27, 345, 824, 1574, 1679, 2006, 2136
BfmI CTRYAG 5 cut(s) 51, 694, 896, 1014, 2025
Bme1390I CCNGG 4 cut(s) 842, 1381, 1597, 1688
Bme18I GGWCC 1 cut(s) 213
BmeRI GACNNNNNGTC 1 cut(s) 1282
BmeT110I CYCGRG 1 cut(s) 2068
BmgBI CACGTC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 4 cut(s) 591, 643, 792, 2195
BmrFI CCNGG 4 cut(s) 842, 1381, 1597, 1688
BmsI GCATC 5 cut(s) 274, 1088, 1226, 1713, 2008
BmtI GCTAGC 1 cut(s) 827
BpiI GAAGAC 3 cut(s) 52, 71, 468
BplI GAGNNNNNCTC 2 cut(s) 1548, 1580
BpmI CTGGAG 1 cut(s) 2231
Bpu10I CCTNAGC 2 cut(s) 1072, 2015
BpuEI CTTGAG 2 cut(s) 853, 1772
Bsa29I ATCGAT 1 cut(s) 336
BsaBI GATNNNNATC 2 cut(s) 91, 147
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 1134
BsaJI CCNNGG 5 cut(s) 216, 593, 794, 1687, 1917
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 1623
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 2 cut(s) 1546, 1628
Bse21I CCTNAGG 1 cut(s) 2102
Bse3DI GCAATG 1 cut(s) 1711
Bse8I GATNNNNATC 2 cut(s) 91, 147
BseBI CCWGG 4 cut(s) 842, 1381, 1597, 1688
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 5 cut(s) 216, 593, 794, 1687, 1917
BseGI GGATG 6 cut(s) 463, 1042, 1111, 1473, 1999, 2177
BseJI GATNNNNATC 2 cut(s) 91, 147
BseLI CCNNNNNNNGG 2 cut(s) 217, 1623
BseMI GCAATG 1 cut(s) 1711
BseMII CTCAG 3 cut(s) 833, 1014, 1086
BseNI ACTGG 2 cut(s) 1546, 1628
BseSI GKGCMC 1 cut(s) 1930
BseXI GCAGC 9 cut(s) 166, 451, 1000, 1022, 1054, 1088, 1253, 1331, 1469
BsgI GTGCAG 1 cut(s) 1281
Bsh1236I CGCG 1 cut(s) 1096
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 7 cut(s) 5, 169, 869, 894, 1627, 1668, 2161
BshNI GGYRCC 2 cut(s) 589, 790
BshVI ATCGAT 1 cut(s) 336
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 4 cut(s) 248, 311, 1175, 1503
BsiHKCI CYCGRG 1 cut(s) 2068
BsiSI CCGG 2 cut(s) 35, 211
BslFI GGGAC 1 cut(s) 1724
BslI CCNNNNNNNGG 2 cut(s) 217, 1623
BsmAI GTCTC 3 cut(s) 657, 1134, 1899
BsmFI GGGAC 1 cut(s) 1724
BsmI GAATGC 1 cut(s) 1033
BsnI GGCC 7 cut(s) 5, 169, 869, 894, 1627, 1668, 2161
Bso31I GGTCTC 1 cut(s) 1134
BsoBI CYCGRG 1 cut(s) 2068
Bsp1286I GDGCHC 5 cut(s) 248, 311, 1175, 1503, 1930
Bsp19I CCATGG 3 cut(s) 216, 593, 794
BspACI CCGC 6 cut(s) 160, 354, 1898, 1997, 2162, 2217
BspANI GGCC 7 cut(s) 5, 169, 869, 894, 1627, 1668, 2161
BspCNI CTCAG 3 cut(s) 832, 1015, 1085
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 1 cut(s) 1096
BspHI TCATGA 2 cut(s) 373, 2062
BspLI GGNNCC 4 cut(s) 591, 643, 792, 2195
BspMAI CTGCAG 1 cut(s) 1018
BspOI GCTAGC 1 cut(s) 827
BspPI GGATC 2 cut(s) 2015, 2067
BspT107I GGYRCC 2 cut(s) 589, 790
BspTNI GGTCTC 1 cut(s) 1134
BsrBI CCGCTC 2 cut(s) 354, 2217
BsrDI GCAATG 1 cut(s) 1711
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 2 cut(s) 1546, 1628
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 5 cut(s) 216, 593, 794, 1687, 1917
BssNI GRCGYC 1 cut(s) 57
BssSI CACGAG 2 cut(s) 292, 972
BssT1I CCWWGG 4 cut(s) 216, 593, 794, 1917
Bst2BI CACGAG 2 cut(s) 292, 972
Bst2UI CCWGG 4 cut(s) 842, 1381, 1597, 1688
Bst4CI ACNGT 6 cut(s) 467, 530, 900, 1231, 1331, 1536
Bst6I CTCTTC 7 cut(s) 875, 930, 1400, 1575, 1626, 1914, 1969
BstACI GRCGYC 1 cut(s) 57
BstAPI GCANNNNNTGC 1 cut(s) 1483
BstC8I GCNNGC 5 cut(s) 7, 825, 1094, 1386, 1524
BstDEI CTNAG 6 cut(s) 819, 1023, 1072, 1976, 2015, 2102
BstDSI CCRYGG 3 cut(s) 216, 593, 794
BstEII GGTNACC 1 cut(s) 1284
BstF5I GGATG 6 cut(s) 463, 1042, 1111, 1473, 1999, 2177
BstFNI CGCG 1 cut(s) 1096
BstMAI GTCTC 3 cut(s) 657, 1134, 1899
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 5 cut(s) 185, 1073, 1076, 1483, 1789
BstNI CCWGG 4 cut(s) 842, 1381, 1597, 1688
BstPI GGTNACC 1 cut(s) 1284
BstSCI CCNGG 4 cut(s) 840, 1379, 1595, 1686
BstSFI CTRYAG 5 cut(s) 51, 694, 896, 1014, 2025
BstSLI GKGCMC 1 cut(s) 1930
BstUI CGCG 1 cut(s) 1096
BstV1I GCAGC 9 cut(s) 166, 451, 1000, 1022, 1054, 1088, 1253, 1331, 1469
BstV2I GAAGAC 3 cut(s) 52, 71, 468
BstXI CCANNNNNNTGG 2 cut(s) 397, 2190
Bsu15I ATCGAT 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 2102
BsuRI GGCC 7 cut(s) 5, 169, 869, 894, 1627, 1668, 2161
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 3 cut(s) 216, 593, 794
BtrI CACGTC 1 cut(s) 116
BtsCI GGATG 6 cut(s) 463, 1042, 1111, 1473, 1999, 2177
BtsIMutI CAGTG 2 cut(s) 472, 1539
Cac8I GCNNGC 5 cut(s) 7, 825, 1094, 1386, 1524
CaiI CAGNNNCTG 1 cut(s) 1022
CciI TCATGA 2 cut(s) 373, 2062
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 213
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 3 cut(s) 171, 1365, 1960
DdeI CTNAG 6 cut(s) 819, 1023, 1072, 1976, 2015, 2102
DraI TTTAAA 1 cut(s) 1797
DraIII CACNNNGTG 1 cut(s) 1304
DrdI GACNNNNNNGTC 1 cut(s) 1691
DriI GACNNNNNGTC 1 cut(s) 1282
DseDI GACNNNNNNGTC 1 cut(s) 1691
EaeI YGGCCR 2 cut(s) 1625, 2159
Eam1104I CTCTTC 7 cut(s) 875, 930, 1400, 1575, 1626, 1914, 1969
Eam1105I GACNNNNNGTC 1 cut(s) 1282
EarI CTCTTC 7 cut(s) 875, 930, 1400, 1575, 1626, 1914, 1969
EciI GGCGGA 1 cut(s) 1887
Ecl136II GAGCTC 2 cut(s) 246, 1173
Eco130I CCWWGG 4 cut(s) 216, 593, 794, 1917
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 2 cut(s) 248, 1175
Eco31I GGTCTC 1 cut(s) 1134
Eco47I GGWCC 1 cut(s) 213
Eco53kI GAGCTC 2 cut(s) 246, 1173
Eco57I CTGAAG 4 cut(s) 543, 744, 987, 2102
Eco81I CCTNAGG 1 cut(s) 2102
Eco88I CYCGRG 1 cut(s) 2068
Eco91I GGTNACC 1 cut(s) 1284
EcoICRI GAGCTC 2 cut(s) 246, 1173
EcoO65I GGTNACC 1 cut(s) 1284
EcoRI GAATTC 1 cut(s) 1123
EcoRII CCWGG 4 cut(s) 840, 1379, 1595, 1686
EcoT14I CCWWGG 4 cut(s) 216, 593, 794, 1917
EcoT22I ATGCAT 1 cut(s) 1728
EcoT38I GRGCYC 2 cut(s) 248, 1175
ErhI CCWWGG 4 cut(s) 216, 593, 794, 1917
FaqI GGGAC 1 cut(s) 1724
FauI CCCGC 1 cut(s) 2004
FauNDI CATATG 1 cut(s) 847
FbaI TGATCA 1 cut(s) 958
FokI GGATG 6 cut(s) 470, 1029, 1118, 1480, 1986, 2164
FriOI GRGCYC 2 cut(s) 248, 1175
FspBI CTAG 7 cut(s) 27, 345, 824, 1574, 1679, 2006, 2136
GsuI CTGGAG 1 cut(s) 2231
HaeIII GGCC 7 cut(s) 5, 169, 869, 894, 1627, 1668, 2161
HapII CCGG 2 cut(s) 35, 211
HgaI GACGC 3 cut(s) 171, 1365, 1960
Hin1I GRCGYC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 1888
HinfI GANTC 9 cut(s) 650, 965, 995, 1019, 1354, 1367, 1446, 2066, 2239
HpaII CCGG 2 cut(s) 35, 211
HphI GGTGA 8 cut(s) 368, 494, 500, 1124, 1278, 1638, 2166, 2239
Hpy166II GTNNAC 4 cut(s) 977, 1438, 1539, 1579
Hpy188III TCNNGA 8 cut(s) 374, 476, 681, 734, 832, 962, 2063, 2210
Hpy8I GTNNAC 4 cut(s) 977, 1438, 1539, 1579
HpyCH4III ACNGT 6 cut(s) 467, 530, 900, 1231, 1331, 1536
HpyCH4IV ACGT 2 cut(s) 57, 115
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 1073, 1076, 1483, 1789
HpyF3I CTNAG 6 cut(s) 819, 1023, 1072, 1976, 2015, 2102
HpySE526I ACGT 2 cut(s) 57, 115
Hsp92I GRCGYC 1 cut(s) 57
KpnI GGTACC 2 cut(s) 593, 794
Ksp22I TGATCA 1 cut(s) 958
LmnI GCTCC 5 cut(s) 67, 251, 647, 1170, 1178
Lsp1109I GCAGC 9 cut(s) 166, 451, 1000, 1022, 1054, 1088, 1253, 1331, 1469
LweI GCATC 5 cut(s) 274, 1088, 1226, 1713, 2008
MaeI CTAG 7 cut(s) 27, 345, 824, 1574, 1679, 2006, 2136
MaeII ACGT 2 cut(s) 57, 115
MaeIII GTNAC 9 cut(s) 473, 904, 982, 1097, 1284, 1530, 1587, 1633, 2227
MbiI CCGCTC 2 cut(s) 354, 2217
MfeI CAATTG 1 cut(s) 391
MhlI GDGCHC 5 cut(s) 248, 311, 1175, 1503, 1930
MlyI GAGTC 5 cut(s) 659, 959, 1013, 1361, 2060
MmeI TCCRAC 1 cut(s) 1173
Mph1103I ATGCAT 1 cut(s) 1728
MroXI GAANNNNTTC 1 cut(s) 1127
MseI TTAA 5 cut(s) 917, 1211, 1796, 1988, 2254
MslI CAYNNNNRTG 2 cut(s) 480, 2188
MspA1I CMGCKG 1 cut(s) 1013
MspI CCGG 2 cut(s) 35, 211
MspR9I CCNGG 4 cut(s) 842, 1381, 1597, 1688
MunI CAATTG 1 cut(s) 391
Mva1269I GAATGC 1 cut(s) 1033
MvaI CCWGG 4 cut(s) 842, 1381, 1597, 1688
MvnI CGCG 1 cut(s) 1096
MwoI GCNNNNNNNGC 5 cut(s) 185, 1073, 1076, 1483, 1789
NcoI CCATGG 3 cut(s) 216, 593, 794
NdeI CATATG 1 cut(s) 847
NheI GCTAGC 1 cut(s) 823
NlaIV GGNNCC 4 cut(s) 591, 643, 792, 2195
NmuCI GTSAC 5 cut(s) 473, 1097, 1284, 1633, 2227
NsiI ATGCAT 1 cut(s) 1728
OliI CACNNNNGTG 1 cut(s) 480
PaeR7I CTCGAG 1 cut(s) 2068
PagI TCATGA 2 cut(s) 373, 2062
PceI AGGCCT 1 cut(s) 169
PctI GAATGC 1 cut(s) 1033
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 4 cut(s) 995, 1354, 1446, 2239
Ple19I CGATCG 1 cut(s) 89
PleI GAGTC 5 cut(s) 658, 959, 1013, 1361, 2060
PpsI GAGTC 5 cut(s) 658, 959, 1013, 1361, 2060
PshBI ATTAAT 1 cut(s) 917
PsiI TTATAA 1 cut(s) 1188
Psp124BI GAGCTC 2 cut(s) 248, 1175
Psp6I CCWGG 4 cut(s) 840, 1379, 1595, 1686
PspEI GGTNACC 1 cut(s) 1284
PspGI CCWGG 4 cut(s) 840, 1379, 1595, 1686
PspN4I GGNNCC 4 cut(s) 591, 643, 792, 2195
PspPI GGNCC 1 cut(s) 213
PspXI VCTCGAGB 1 cut(s) 2068
PstI CTGCAG 1 cut(s) 1018
PstNI CAGNNNCTG 1 cut(s) 1022
PvuI CGATCG 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 1013
RgaI GCGATCGC 1 cut(s) 89
RseI CAYNNNNRTG 2 cut(s) 480, 2188
SacI GAGCTC 2 cut(s) 248, 1175
SaqAI TTAA 5 cut(s) 917, 1211, 1796, 1988, 2254
Sau96I GGNCC 1 cut(s) 213
SchI GAGTC 5 cut(s) 659, 959, 1013, 1361, 2060
ScrFI CCNGG 4 cut(s) 842, 1381, 1597, 1688
SduI GDGCHC 5 cut(s) 248, 311, 1175, 1503, 1930
SfaAI GCGATCGC 1 cut(s) 89
SfaNI GCATC 5 cut(s) 274, 1088, 1226, 1713, 2008
SfcI CTRYAG 5 cut(s) 51, 694, 896, 1014, 2025
Sfr274I CTCGAG 1 cut(s) 2068
SgfI GCGATCGC 1 cut(s) 89
SinI GGWCC 1 cut(s) 213
SlaI CTCGAG 1 cut(s) 2068
SmiMI CAYNNNNRTG 2 cut(s) 480, 2188
SmlI CTYRAG 3 cut(s) 832, 1787, 2068
SmoI CTYRAG 3 cut(s) 832, 1787, 2068
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 6 cut(s) 160, 354, 1898, 1997, 2162, 2217
SspI AATATT 3 cut(s) 124, 1842, 1866
SspMI CTAG 7 cut(s) 27, 345, 824, 1574, 1679, 2006, 2136
SstI GAGCTC 2 cut(s) 248, 1175
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 4 cut(s) 840, 1379, 1595, 1686
StyI CCWWGG 4 cut(s) 216, 593, 794, 1917
TaaI ACNGT 6 cut(s) 467, 530, 900, 1231, 1331, 1536
TaiI ACGT 2 cut(s) 60, 118
TaqI TCGA 6 cut(s) 242, 336, 1497, 1695, 1810, 2069
TaqII GACCGA 1 cut(s) 1272
TatI WGTACW 4 cut(s) 530, 1331, 1429, 1845
TauI GCSGC 2 cut(s) 2164, 2220
TfiI GAWTC 4 cut(s) 995, 1354, 1446, 2239
Tru1I TTAA 5 cut(s) 917, 1211, 1796, 1988, 2254
Tru9I TTAA 5 cut(s) 917, 1211, 1796, 1988, 2254
TscAI CASTG 2 cut(s) 472, 1546
TseFI GTSAC 5 cut(s) 473, 1097, 1284, 1633, 2227
TseI GCWGC 9 cut(s) 179, 439, 1010, 1013, 1067, 1076, 1241, 1344, 1457
Tsp45I GTSAC 5 cut(s) 473, 1097, 1284, 1633, 2227
TspGWI ACGGA 1 cut(s) 724
TspRI CASTG 2 cut(s) 472, 1546
VpaK11BI GGWCC 1 cut(s) 213
VspI ATTAAT 1 cut(s) 917
XapI RAATTY 3 cut(s) 1123, 1412, 1990
XhoI CTCGAG 1 cut(s) 2068
XmnI GAANNNNTTC 1 cut(s) 1127
XspI CTAG 7 cut(s) 27, 345, 824, 1574, 1679, 2006, 2136
ZraI GACGTC 1 cut(s) 58
Zsp2I ATGCAT 1 cut(s) 1728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.